Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,579,564 G→A 17.7% intergenic (‑222/+69) yddA ← / ← ydeM putative multidrug ABC transporter permease/ATPase/putative YdeN‑specific sulfatase‑maturating enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,579,5640GA17.7% 245.7 / 40.8 107intergenic (‑222/+69)yddA/ydeMputative multidrug ABC transporter permease/ATPase/putative YdeN‑specific sulfatase‑maturating enzyme
Reads supporting (aligned to +/- strand):  ref base G (37/51);  new base A (8/11);  total (45/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

Only 100 of 112 total aligned reads displayed.

TATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCC  >  NC_000913/1579465‑1579663
                                                                                                   |                                                                                                   
tatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAgg                                                                                                     >  2:2340205/1‑100 (MQ=255)
tatgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAgg                                                                                                     <  2:284606/100‑1 (MQ=255)
  tgCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGaa                                                                                                   >  1:1788019/1‑100 (MQ=255)
    cTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGTAAGATGATGATGACGAAACACCCGACAGGaagt                                                                                                 >  2:1927018/1‑98 (MQ=255)
      aTTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACGCCCGACAGGAATTTa                                                                                               >  1:2520104/1‑100 (MQ=255)
      aTTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGGAAGCCAGGATTTTCGAAAGGGAAGATGATGATGACGAAACACCCGACAGGAAttt                                                                                                <  2:1980945/99‑1 (MQ=255)
       ttGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTAt                                                                                              <  1:876083/100‑1 (MQ=255)
        tGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATg                                                                                             >  2:1253369/1‑100 (MQ=255)
        tGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATg                                                                                             <  2:2214751/100‑1 (MQ=255)
         gATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGt                                                                                            >  2:1675281/1‑100 (MQ=255)
         gATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGt                                                                                            >  2:22322/1‑100 (MQ=255)
           tAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCg                                                                                          <  1:1060245/100‑1 (MQ=255)
            aaTCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCgg                                                                                         >  1:1617816/1‑100 (MQ=255)
             aTCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACCCCCGACAGGAATTTATGTCggg                                                                                        <  2:1914097/100‑1 (MQ=255)
              tCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGt                                                                                       <  2:1924648/100‑1 (MQ=255)
              tCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGt                                                                                       <  2:528287/100‑1 (MQ=255)
              tCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGt                                                                                       <  1:2287845/100‑1 (MQ=255)
              tCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGt                                                                                       <  1:2198689/100‑1 (MQ=255)
              tCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGt                                                                                       <  1:2141916/100‑1 (MQ=255)
              tCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGt                                                                                       <  2:490565/100‑1 (MQ=255)
              tCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGt                                                                                       <  1:745820/100‑1 (MQ=255)
              tCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGt                                                                                       <  1:2218127/100‑1 (MQ=255)
               cAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATTTCGGGTg                                                                                      <  1:1350305/100‑1 (MQ=255)
               cAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTg                                                                                      <  1:1843814/100‑1 (MQ=255)
                aaGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGTgt                                                                                     <  1:2345974/100‑2 (MQ=38)
                aaGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGTgt                                                                                     <  1:2345961/100‑2 (MQ=38)
                aaGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGc                                                                                     >  2:659872/1‑100 (MQ=255)
                     tatTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGt                                                                                <  1:765410/100‑1 (MQ=255)
                     tatTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGt                                                                                >  1:202952/1‑100 (MQ=255)
                     tatTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGt                                                                                <  2:804566/100‑1 (MQ=255)
                       ttGCTAATGCCCTGATGCATACCATGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACATAAATTCCTGTCGGGTgtttcgt                                                                                <  2:1115471/98‑7 (MQ=25)
                       ttGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGtt                                                                               >  2:2216678/1‑99 (MQ=255)
                        tGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTaa                                                                             >  1:1968000/1‑100 (MQ=255)
                         gCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAg                                                                            >  1:1964984/1‑100 (MQ=255)
                          cTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAgg                                                                           >  2:1640492/1‑100 (MQ=255)
                          cTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAgg                                                                           <  1:1611693/100‑1 (MQ=255)
                          cTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAgg                                                                           <  2:378384/100‑1 (MQ=255)
                           tAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGt                                                                          <  1:898051/100‑1 (MQ=255)
                           tAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGt                                                                          <  1:39030/100‑1 (MQ=255)
                              tGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCAt                                                                       >  2:1512682/1‑100 (MQ=255)
                                cccTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATaa                                                                     <  2:133761/100‑1 (MQ=255)
                                cccTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATaa                                                                     >  1:1871889/1‑100 (MQ=255)
                                 ccTGATGCATACCACGTAAGCCAGGATTTTCGCCAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATaag                                                                    >  2:1595778/1‑100 (MQ=255)
                                 ccTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATaa                                                                     >  1:841815/1‑99 (MQ=255)
                                    gATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATaagaag                                                                 >  2:1278213/1‑100 (MQ=255)
                                    gATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATaagaag                                                                 <  1:1540485/100‑1 (MQ=255)
                                    gATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATaagaa                                                                  >  2:67114/1‑99 (MQ=255)
                                    gATGCATACCACGTAAGCCAGGATTTTCACAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATaaga                                                                   <  2:510274/98‑1 (MQ=255)
                                      tGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAagga                                                               >  1:1934574/1‑100 (MQ=255)
                                       gCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAaggag                                                              <  1:969098/100‑1 (MQ=255)
                                        cATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAaggagg                                                             >  2:599054/1‑100 (MQ=255)
                                         aTACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGc                                                            <  2:1301681/100‑1 (MQ=255)
                                         aTACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGc                                                            <  2:649327/100‑1 (MQ=255)
                                         aTACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGc                                                            <  1:920167/100‑1 (MQ=255)
                                         aTACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGc                                                            <  1:22323/100‑1 (MQ=255)
                                           aCCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGc                                                            <  1:2179193/98‑1 (MQ=255)
                                            ccACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTaa                                                         >  2:497116/1‑100 (MQ=255)
                                             cACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAg                                                        <  1:2085922/100‑1 (MQ=255)
                                              aCGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGa                                                       >  1:1140854/1‑100 (MQ=255)
                                                  aaGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATgg                                                   <  1:2214384/100‑1 (MQ=255)
                                                    gCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAg                                                 >  2:2547556/1‑100 (MQ=255)
                                                    gCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAg                                                 <  2:1779916/100‑1 (MQ=255)
                                                    gCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAg                                                 <  1:1085031/100‑1 (MQ=255)
                                                    gCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAg                                                 <  1:1912399/100‑1 (MQ=255)
                                                     ccAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGt                                                >  1:2157531/1‑100 (MQ=255)
                                                         gATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATCAGAAGGAGGCTAAGAATGGAGTTaaa                                            <  1:1561415/100‑1 (MQ=255)
                                                          aTTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCGTGTTAAGGTCACAAGAAGGAGGCTAAGAATGGAGTTaaaa                                           <  2:515883/100‑1 (MQ=255)
                                                               cGCAAGGGAAGATGATGATGACGAAAAACCAGACAGGAATTTATGTAGGGTGCCTAGTTAAGGTCATGAGAAGGTGGATAAGAATGGAATTAAAagagag                                      >  2:226237/1‑100 (MQ=255)
                                                                 cAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCg                                    >  1:1753031/1‑100 (MQ=255)
                                                                   atggAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGTTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGtt                                  <  2:2507624/98‑1 (MQ=255)
                                                                    gggAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGtta                                 <  2:2097408/100‑1 (MQ=255)
                                                                      gAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGttatt                               <  1:196701/100‑1 (MQ=255)
                                                                           tgatgatgaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTattaatta                          >  1:519389/1‑100 (MQ=255)
                                                                                atgaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATtctc                     >  1:24886/1‑100 (MQ=255)
                                                                                 tgaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATtctcn                    <  1:1275631/100‑2 (MQ=255)
                                                                                 tgaCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTcc                    >  2:1245328/1‑100 (MQ=255)
                                                                                   acaaggcacCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCAt                  >  2:453148/7‑100 (MQ=37)
                                                                                   aCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCAt                  <  2:2157517/100‑1 (MQ=255)
                                                                                   aCGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCAt                  <  2:1033793/100‑1 (MQ=255)
                                                                                    cGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCAtt                 >  2:322701/1‑100 (MQ=255)
                                                                                      aaaCACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTg               <  1:1245336/100‑1 (MQ=255)
                                                                                      aaaCACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTg               >  1:1806636/1‑100 (MQ=255)
                                                                                        gcacCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGtt             <  1:453152/99‑1 (MQ=38)
                                                                                        gcacCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGtt             >  2:2275948/2‑100 (MQ=38)
                                                                                        gcacCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGtt             >  2:2503660/2‑100 (MQ=38)
                                                                                        gcacCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGtt             >  2:2549832/2‑100 (MQ=38)
                                                                                         nacCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGCCAAAAGAAGGCGGCTAAGAATGGAGTTAAAAGAGAGCTTTATTACTTATTCTACATTTGttt            >  2:790471/2‑100 (MQ=255)
                                                                                         cacCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGttt            >  2:1442208/1‑100 (MQ=38)
                                                                                         cacCCGACATAAATTCCTGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGttt            >  1:1987630/1‑100 (MQ=38)
                                                                                          acCCTACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGTATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGtttt           <  2:883278/100‑1 (MQ=255)
                                                                                           cccGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATTGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGtttt           >  2:16082/1‑99 (MQ=255)
                                                                                            ccGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGc         <  2:800481/100‑1 (MQ=255)
                                                                                             cGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCa        >  2:2333834/1‑100 (MQ=255)
                                                                                              gACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCaa       <  1:1150400/100‑1 (MQ=255)
                                                                                              gACAGGAATTTATGTCAGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAAGTATTCTCCATTTGTTTTGCaa       <  2:1317107/100‑1 (MQ=255)
                                                                                               aCAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCaa       <  2:2463681/99‑1 (MQ=255)
                                                                                                 aGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACAt    <  1:1978670/100‑1 (MQ=255)
                                                                                                  ggAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACAt    >  2:257271/1‑99 (MQ=255)
                                                                                                  ggAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATc   <  1:1601794/100‑1 (MQ=255)
                                                                                                   gAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATcc  <  1:497122/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
TATGCTATTGATAATCAAGGATATTGCTAATGCCCTGATGCATACCACGTAAGCCAGGATTTTCGCAAGGGAAGATGATGATGACGAAACACCCGACAGGAATTTATGTCGGGTGCCTTGTTAAGGTCATAAGAAGGAGGCTAAGAATGGAGTTAAAAGAGAGCGTTATTAATTATTCTCCATTTGTTTTGCAACATCC  >  NC_000913/1579465‑1579663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: