Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 162,973 G→A 100% C290Y (TGT→TAT)  hrpB → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913162,9730GA98.2% 369.2 / ‑2.6 113C290Y (TGT→TAT) hrpBputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (48/63);  total (49/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01

Only 100 of 114 total aligned reads displayed.

CGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCG  >  NC_000913/162874‑163068
                                                                                                   |                                                                                               
cGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATtg                                                                                                 <  1:1900680/100‑1 (MQ=255)
    cctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGcc                                                                                             <  1:252935/100‑1 (MQ=255)
      tccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCa                                                                                           <  2:34512/100‑1 (MQ=255)
      tccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCa                                                                                           <  2:1084505/100‑1 (MQ=255)
       cccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAg                                                                                          <  1:1161425/100‑1 (MQ=255)
        ccGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAgg                                                                                         <  2:421082/100‑1 (MQ=255)
            cACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCg                                                                                     <  1:1925322/100‑1 (MQ=255)
             aCCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgt                                                                                    <  1:1846100/100‑1 (MQ=255)
              ccGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgtg                                                                                   >  2:1898888/1‑100 (MQ=255)
              ccGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCgtg                                                                                   >  2:1916668/1‑100 (MQ=255)
                gCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGtgtg                                                                                 <  1:2162763/100‑1 (MQ=255)
                 cAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTgg                                                                                <  1:1756440/100‑1 (MQ=255)
                  aaGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgc                                                                               <  2:113520/100‑1 (MQ=255)
                  aaGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgc                                                                               <  1:172844/100‑1 (MQ=255)
                   aGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgc                                                                               <  2:1703872/99‑1 (MQ=255)
                    gggATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgcgc                                                                             >  1:98494/1‑100 (MQ=255)
                    gggATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGgcgc                                                                             >  1:1334455/1‑100 (MQ=255)
                      gATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGt                                                                           >  1:1935500/1‑100 (MQ=255)
                      gATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGGGGCGCGt                                                                           <  1:1344958/100‑1 (MQ=255)
                       aTGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtt                                                                          >  1:1664962/1‑100 (MQ=255)
                        tGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGttt                                                                         >  1:1658181/1‑100 (MQ=255)
                        tGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGttt                                                                         <  2:41555/100‑1 (MQ=255)
                        tGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGttt                                                                         <  1:1092613/100‑1 (MQ=255)
                         gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                        <  1:1482317/100‑1 (MQ=255)
                         gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                        <  2:232963/100‑1 (MQ=255)
                         gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                        <  1:2490253/100‑1 (MQ=255)
                         gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                        <  1:2237726/100‑1 (MQ=255)
                         gcgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGtttt                                                                        >  2:264606/1‑100 (MQ=255)
                          cgcAAAGTGGTGCTGGCGACCTATATTGCTGAAGCTAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTGTTg                                                                       <  2:244007/100‑1 (MQ=255)
                          cgcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTCGCGCGTTTTg                                                                       >  2:2315386/1‑100 (MQ=255)
                           gcAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGa                                                                      <  2:774679/100‑1 (MQ=255)
                             aaaGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATc                                                                    <  1:29022/100‑1 (MQ=255)
                               cgatctgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcg                                                                  <  1:764136/95‑1 (MQ=255)
                               aGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcg                                                                  <  1:696972/100‑1 (MQ=255)
                                 tggtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcgcg                                                                <  1:2283598/100‑1 (MQ=255)
                                 tggtgCTGGAGACCAATATTTTTTATACCAGTTTAACCATTGAAGGTAGTCGTCTGGTGGTGGATTATGCTCAGGAGCGTGTGGCGCGTTTTGATCcgcg                                                                <  2:927729/100‑1 (MQ=255)
                                  ggtgCTGGAGAGCAATATTGCTGAATCTAGTTTAACCATTGGCGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGGTTTGATCcgcg                                                                <  2:2136069/99‑1 (MQ=255)
                                   gtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                              >  1:172872/1‑100 (MQ=255)
                                   gtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                              >  1:84478/1‑100 (MQ=255)
                                   gtgCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATCCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCa                                                              >  1:773338/1‑100 (MQ=255)
                                     gCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCcgagatcg                                                            >  2:764129/1‑94 (MQ=255)
                                     gCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTCGATTATGCCCTGGAGCGTGTGGCGCGTTTTGATCCGCGCaag                                                            <  1:1731542/100‑3 (MQ=255)
                                      cTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACgg                                                           >  1:1145911/1‑100 (MQ=255)
                                       tGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACggg                                                          >  1:534888/1‑100 (MQ=255)
                                         gCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGc                                                        <  1:1123376/100‑1 (MQ=255)
                                         gCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGc                                                        <  1:1931618/100‑1 (MQ=255)
                                          cGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCt                                                       >  2:1058377/1‑100 (MQ=255)
                                           gACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCtt                                                      <  2:1156679/100‑1 (MQ=255)
                                            aCCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTa                                                     <  1:1354638/100‑1 (MQ=255)
                                            aCCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTa                                                     <  1:1775557/100‑1 (MQ=255)
                                            aCCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTa                                                     >  1:2036045/1‑100 (MQ=255)
                                             ccAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAc                                                    <  2:929908/100‑1 (MQ=255)
                                              cAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTAcg                                                   >  2:1010862/1‑100 (MQ=255)
                                                  atTGCTGAAACCAGTTTAACCATTTAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGAc                                               <  1:941262/100‑1 (MQ=255)
                                                    tGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTg                                             <  1:2374638/100‑1 (MQ=255)
                                                     gCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGa                                            >  2:1945700/1‑100 (MQ=255)
                                                     gCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGa                                            <  1:1499163/100‑1 (MQ=255)
                                                     gCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGa                                            >  2:1139106/1‑100 (MQ=255)
                                                       tGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGAtt                                          <  1:1984217/100‑1 (MQ=255)
                                                         aaaCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTAc                                        <  1:2304840/100‑1 (MQ=255)
                                                           aCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGTCGCGTTTTGATCCGCGCACGGGGCTTACACGACTGATTAcac                                      <  1:1733847/100‑3 (MQ=255)
                                                           aCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTc                                      <  2:2374992/100‑1 (MQ=255)
                                                           aCCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTc                                      <  2:309964/100‑1 (MQ=255)
                                                            ccAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCa                                     >  2:1776095/1‑100 (MQ=255)
                                                              aGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAc                                   <  1:924807/100‑1 (MQ=255)
                                                               gTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCaa                                    >  2:917531/1‑98 (MQ=255)
                                                               gTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcg                                  >  2:917881/1‑100 (MQ=255)
                                                                tttAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcgc                                 <  2:1456382/100‑1 (MQ=255)
                                                                 ttAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAAcgcg                                <  1:38242/100‑1 (MQ=255)
                                                                  tAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGTAGCGTGTGGCGCGTTTTGATCCGCGAACGGGGCTTACGCGACTGATTACTCAACGCGt                               >  2:1799725/1‑100 (MQ=255)
                                                                  tAACCATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGt                               >  2:1764994/1‑100 (MQ=255)
                                                                   aaCCATTGAAGGTATTCGTCTGGTGGTGGATAATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGtt                              >  1:1730333/1‑100 (MQ=255)
                                                                   aaCCAGTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGtt                              <  2:879065/100‑1 (MQ=255)
                                                                     ccATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGTGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAg                            <  2:1799363/100‑1 (MQ=255)
                                                                     ccATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAg                            >  2:2401275/1‑100 (MQ=255)
                                                                     ccATTGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAg                            >  1:1432448/1‑100 (MQ=255)
                                                                         tGAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAg                        >  1:1245063/1‑100 (MQ=255)
                                                                          gAAGGTATTCGTCTGGTGGTGGATTATGCCGAGGAGGGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAGCGCGTTAGCCAg                        <  2:1248856/99‑1 (MQ=255)
                                                                          gAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                       >  2:309444/1‑100 (MQ=255)
                                                                          gAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                       <  1:1433723/100‑1 (MQ=255)
                                                                          gAAGGTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAgg                       >  1:1318484/1‑100 (MQ=255)
                                                                             ggTATTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCAt                    <  1:2401289/100‑1 (MQ=255)
                                                                               tATTCGTCTGGTGGTGGATTATGCCCATGAGCTTGTGGCGCGTTTTGACCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATcc                  <  2:1947039/100‑1 (MQ=255)
                                                                                aTTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCa                 <  1:1345437/100‑1 (MQ=255)
                                                                                aTTCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCa                 <  2:1354461/100‑1 (MQ=255)
                                                                                 ttCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCAt                >  1:1746764/1‑100 (MQ=255)
                                                                                 ttCGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCAt                <  1:863357/100‑1 (MQ=255)
                                                                                   cGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGa              <  2:1649653/100‑1 (MQ=255)
                                                                                   cGTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTGTGATCCGCGCACGGGGCTTACGCGACTGATAACTCAACGCGTTAGCCAGGCATCCATGa              >  1:58375/1‑100 (MQ=255)
                                                                                    gTCTGGTGGTGGATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGAc             <  1:2400142/100‑1 (MQ=255)
                                                                                       tggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGAAGCa          >  2:1698815/1‑100 (MQ=255)
                                                                                        ggtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAg         >  2:1474290/1‑100 (MQ=255)
                                                                                         gtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGc        >  1:10582/1‑100 (MQ=255)
                                                                                         gtggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGc        >  2:281930/1‑100 (MQ=255)
                                                                                          tggtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCg       <  2:2521808/100‑1 (MQ=255)
                                                                                            gtggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTg     >  1:1745837/1‑100 (MQ=255)
                                                                                             tggATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGc    >  1:2267138/1‑100 (MQ=255)
                                                                                              ggATTATGCCCAGGAGCGTGTGGCGCGTTTTGAGCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGcc   <  2:2004123/100‑1 (MQ=255)
                                                                                               gATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCg  >  1:1254246/1‑100 (MQ=255)
                                                                                               gATTATGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCg  <  1:1853663/100‑1 (MQ=255)
                                                                                                   |                                                                                               
CGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGCGTGTGGCGCGTTTTGATCCGCGCACGGGGCTTACGCGACTGATTACTCAACGCGTTAGCCAGGCATCCATGACGCAGCGTGCCG  >  NC_000913/162874‑163068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: