Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,658,592 | C→T | 100% | A175V (GCC→GTC) | ynfE → | putative selenate reductase, periplasmic |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,658,592 | 0 | C | T | 100.0% | 267.3 / ‑8.1 | 88 | A175V (GCC→GTC) | ynfE | putative selenate reductase, periplasmic |
Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (30/55); minor base A (0/2); total (30/58) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.43e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
CTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACAC > NC_000913/1658495‑1658688 | cTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCt > 2:551253/1‑100 (MQ=255) cTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCt > 1:593681/1‑100 (MQ=255) gaagaaAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTcg < 2:289970/100‑1 (MQ=255) agaaAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTcggc < 1:2497758/100‑1 (MQ=255) aaaaCCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTcggcgg > 1:1798818/1‑100 (MQ=255) aaaCCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGt < 1:2498869/100‑1 (MQ=255) acccGTCGAACAATATGGCAATGAGGCTGTATATACTCAGTACTCTTCGGGAATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTcggcgg < 2:1216164/96‑1 (MQ=255) gTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAAc > 2:533271/1‑100 (MQ=255) gTCGAACAATATGGCAATGAGGCGGTAAATATTCAGGCCTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAAc < 2:593674/100‑1 (MQ=255) cGAGCAGTATGGAGATGAGGGTGTAAATATACAGCACTGTTGGTGTATCTATTGCGGCAATATGACCCGCTGTTCGCCATCAGTCTCGGCGGTCaaa < 2:100159/97‑1 (MQ=255) aaCAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCt > 2:542265/1‑100 (MQ=255) aaTATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCtgat > 2:1452346/1‑100 (MQ=255) atatGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCtgatg > 2:849261/1‑100 (MQ=255) atatGGAGATGAGGCAGAACATATTCACAACTCTTCGGCGATCCTTTGCGGCTACAGGACCCGCTCTTCGCCGTCAGTCTCGGCGGTCAAACGCCtga < 2:768588/98‑1 (MQ=255) atGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCATTGGCGGCAATATGACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGaa < 2:1416541/100‑1 (MQ=255) ggCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACt > 2:71906/1‑100 (MQ=255) ggCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACt > 1:1562208/1‑100 (MQ=255) gCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTg > 1:583254/1‑100 (MQ=255) gCAATGAGGCGGTATACATCCAATACTCTTCCGGGATCGTTGGCGGCAATAAGACCCGTTCTTCGCCATCAGTCTCGGCGGTCAAACGCCtgat < 2:202187/94‑1 (MQ=255) gCAATGAGGCGATATATGTTCATTACTCTTCCGATATCGTTGGCGGCAATAAGACCCGTTCTTCGCCATCAGTCTCGGCGGTCAAACGCCtgat < 2:632835/94‑1 (MQ=255) cAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGc < 1:962440/100‑1 (MQ=255) cAATGAGCATCTATATATTCAGCACTCTCCGGGGATCCTTGGCGGCAATATGACCCGCTCTTCGCCGTCAGTCTCGGCGGTCAAACGCCTGATGAACTGc < 2:466074/100‑1 (MQ=255) aaggAGGCTGAATATATTCATCCATCATTGTGGAACGGTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAAc < 2:1771438/93‑1 (MQ=255) aTGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTa < 1:1605747/100‑1 (MQ=255) ggCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTAcggc < 1:1336009/100‑1 (MQ=255) gTATATATTCAGTACTCTTCGGGGATCGTGGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTAcggcgg < 2:1075068/98‑1 (MQ=255) tatatcTTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTc < 1:1014895/100‑1 (MQ=255) atatatCTAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTc < 2:1562197/99‑1 (MQ=255) tatatTCAGTACTCTTCGTGGATCGTTGGCGGCAATATGACCCGCTTTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGATACGACGGTTCAc > 2:958607/1‑100 (MQ=255) atatTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACt < 1:1427418/100‑1 (MQ=255) tCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAAc < 2:1448459/100‑1 (MQ=255) acagccctctTCTGAGCTCGGTAGCGGCAATAGGACCCGCTCTTCGCCATCAGTCTCGCCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCAc < 2:709930/89‑1 (MQ=255) cAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAAcc < 1:2075579/100‑1 (MQ=255) cAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAAcc < 1:1561604/100‑1 (MQ=255) gTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAg > 2:625551/1‑100 (MQ=255) tACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGt < 1:579318/100‑1 (MQ=255) ctctTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTa < 2:1510134/99‑1 (MQ=255) ctctTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTAt > 1:633388/1‑100 (MQ=255) tctTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATg < 2:1313371/100‑1 (MQ=255) tctTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATg < 1:405231/100‑1 (MQ=255) tctTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATg < 2:1313277/100‑1 (MQ=255) tcgtCGGGGATCGTTAGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATg < 2:2058950/97‑1 (MQ=255) ctTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATgg < 1:1081662/100‑1 (MQ=255) ctTCGGGGATCGTTAGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATgg < 2:436011/100‑1 (MQ=255) cGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTc > 1:237692/1‑100 (MQ=255) ggggATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTcc > 2:1337793/1‑100 (MQ=255) ggggATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTcc < 2:1606853/100‑1 (MQ=255) gcggATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTcc < 2:1552964/98‑1 (MQ=255) gATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTAc < 1:1252119/100‑1 (MQ=255) tCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAg < 1:399800/100‑1 (MQ=255) cGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGc < 2:2427317/100‑1 (MQ=255) gTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCa > 2:170881/1‑100 (MQ=255) gTTGGCGGCAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCa < 1:542272/100‑1 (MQ=255) ttGGCGGCAATATGACCCGCTCTTCGCCATCAGTCGCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCAc < 2:1890960/100‑1 (MQ=255) gggggcAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACa < 2:1097669/91‑1 (MQ=255) ggcggcAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTg > 1:1100276/1‑100 (MQ=255) gcggcAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTgc > 2:2281697/1‑100 (MQ=255) gcggcAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTgc < 1:170883/100‑1 (MQ=255) gcAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCa > 1:1205207/1‑100 (MQ=255) gcAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCa < 1:1539320/100‑1 (MQ=255) cAATATGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCaa > 2:551694/1‑100 (MQ=255) atatGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAAt > 1:1920209/1‑100 (MQ=255) tatGACGCGCCCTGAGCCAACCGACCCGGCGGTCAAACGCTTGATGCGCTGCTACGGCGGTTCACTCAACGAGTATGGCTGCTACAGCACTGCGCAAAtt < 2:176247/100‑1 (MQ=255) tatGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAAtt > 1:1066276/1‑100 (MQ=255) tGACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTc < 1:1360695/100‑1 (MQ=255) tGACACGCTTTTCGCAATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCaaa < 2:841528/96‑1 (MQ=255) gACCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTcc > 1:566282/1‑100 (MQ=255) aCCCGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCt > 2:2213563/1‑100 (MQ=255) cccGCTCTTCGCCAACAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCt < 2:2071645/99‑1 (MQ=255) cGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCtgtg < 1:134146/100‑1 (MQ=255) cGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCtgtg < 1:1429132/100‑1 (MQ=255) cGCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCtgt > 1:1954442/1‑99 (MQ=255) gcgctTCGACATTAGACTCGGCGGTCAAACGCCTGAGGAACTGCTACGGCTGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGc < 2:2364437/97‑1 (MQ=255) gCTCTTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCTTACAGCACTGCGCAAATTTCCTGTGc < 1:1556270/100‑1 (MQ=255) ctctTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGcc < 1:1321553/100‑1 (MQ=255) tctTCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCa < 1:20085/100‑1 (MQ=255) tCGCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGc < 2:79292/100‑1 (MQ=255) tCGCCATCAGTCTCGGCGGGCAAACGCCTGATGAACTGCTTCGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGCGCCATGc < 2:129794/100‑1 (MQ=255) tCGCCATCAGTCTAGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGc > 2:1588986/1‑100 (MQ=255) gCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGccc < 2:583247/100‑1 (MQ=255) gCCATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTAAAGCACTGCGCAAATTTCCTGTACCATGccc > 2:536920/1‑100 (MQ=255) cATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTa > 1:209324/1‑100 (MQ=255) cATCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTa > 1:1384767/1‑100 (MQ=255) tCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTaca < 1:2087043/100‑1 (MQ=255) tCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTaca < 1:2306631/100‑1 (MQ=255) tCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTaca < 1:529956/100‑1 (MQ=255) tCAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTaca > 2:1820464/1‑100 (MQ=255) cAGTCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTGACTCAACCAGTGTGGCTCCTACAGCACTGCTCATATTTCCAGTGCCATGCTCTacac < 2:95357/100‑1 (MQ=255) | CTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACAC > NC_000913/1658495‑1658688 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |