Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,865,682 G→C 15.3% intergenic (‑46/+44) yeaE ← / ← mipA aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,865,6820GC15.3% 285.6 / 38.0 105intergenic (‑46/+44)yeaE/mipAaldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
Reads supporting (aligned to +/- strand):  ref base G (42/46);  new base C (7/9);  total (49/56)
Fisher's exact test for biased strand distribution p-value = 7.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 105 total aligned reads displayed.

CCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACC  >  NC_000913/1865584‑1865780
                                                                                                  |                                                                                                  
 cTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACTGGGCGTGAc                                                                                                  >  2:2230487/1‑100 (MQ=255)
 cTACGGCTGGCAGTGAGACATCGCCACTAAATCGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGAc                                                                                                  <  1:1310381/100‑1 (MQ=255)
   aCGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGc                                                                                                <  2:1847474/100‑1 (MQ=255)
     ggCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGccc                                                                                              <  2:1117588/100‑1 (MQ=255)
     ggCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGcc                                                                                               <  2:199182/99‑1 (MQ=255)
      gCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGc                                                                                                >  2:1761686/1‑97 (MQ=255)
      gCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCt                                                                                             >  2:441711/1‑100 (MQ=255)
       cTGGCAGTGAGACATCGCCACTAAATTGAATCATTATTGGTTGCATTGTTTCCTCCCAGGTATTGCAAAAAAAAAAAAAAAAACAGGGCGTGACGccc                                                                                              <  2:371512/98‑1 (MQ=255)
        tGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACcccc                                                                                              >  2:2213134/1‑97 (MQ=255)
         ggCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTg                                                                                            >  2:2320374/1‑98 (MQ=255)
         ggCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGtt                                                                                          >  1:2095096/1‑100 (MQ=255)
          gCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGttt                                                                                         <  2:887528/100‑1 (MQ=255)
          gCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttt                                                                                         <  2:1292577/100‑1 (MQ=255)
          gCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttt                                                                                         <  1:1458900/100‑1 (MQ=255)
           cAGTGAGACATCGCCACTAAATTGAATGATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtttt                                                                                        <  1:629393/100‑1 (MQ=255)
            aGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGttttt                                                                                       <  1:2320388/100‑1 (MQ=255)
              tGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTAt                                                                                     <  2:2004961/100‑1 (MQ=255)
              tGAGACATCGCCACTAAATTGAATCATTTTTTGTAGCATTGTTTCCTCCCAGGTATTGCACCACCGCAATGCAAAACAGGGCGTCACGCCCTGttttgca                                                                                     <  2:1348446/100‑4 (MQ=38)
              gatctcATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGAAAAACAGGGCGTCACGCCCTGttttgca                                                                                     <  2:1929482/95‑4 (MQ=38)
               gagaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATg                                                                                    <  2:2194672/100‑1 (MQ=255)
               gagaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgcat                                                                                    <  1:474048/100‑5 (MQ=255)
               gagaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgcat                                                                                    <  1:2476678/100‑5 (MQ=255)
                agaCATCGTCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCTCCACCGTAATGCAAAACAGGGCGTGACGTCCTGTTTTTATGc                                                                                   <  2:825128/100‑1 (MQ=255)
                agaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACTACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATg                                                                                    <  2:221272/99‑1 (MQ=255)
                agaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCGCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgcat                                                                                    <  2:786294/99‑5 (MQ=255)
                 gaCATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATACAAAACAGAGCGTGACGACCTCTTTTTATGca                                                                                  >  2:921825/1‑100 (MQ=255)
                   cATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGcaca                                                                                <  1:1773584/100‑1 (MQ=255)
                   cATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGAAAAACAGGGCGTCACGCCCTGttttgcaagatc                                                                                >  1:1929496/1‑92 (MQ=38)
                    aTCGCCACTAAATTGAATCATTTTTTGTTGCATTTTTTCCTCCCATGTATTCCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGcac                                                                                 >  2:1509647/1‑98 (MQ=255)
                    aTCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGcaca                                                                                >  2:1328531/1‑99 (MQ=255)
                     tCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaa                                                                              <  1:2410676/100‑1 (MQ=255)
                         cACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTCCACCACCCTACTGCAAAACAGGGAGTGCCGCCCTGTTTTTCTGcaca                                                                                >  2:1290560/1‑94 (MQ=255)
                          aCTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGcc                                                                         <  1:177625/100‑1 (MQ=255)
                           cTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGccc                                                                        >  1:336337/1‑100 (MQ=255)
                             aaaTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTg                                                                      >  1:1476477/1‑100 (MQ=255)
                                  gAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAg                                                                 >  1:1834266/1‑100 (MQ=255)
                                   aaTCATTCTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGa                                                                >  1:1343984/1‑100 (MQ=255)
                                     tCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATg                                                              <  1:1830107/100‑1 (MQ=255)
                                       aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAACGCCAAACAGGGAGTAACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCa                                                            <  2:1761395/100‑1 (MQ=255)
                                         tttttGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCAtt                                                          <  2:797388/100‑1 (MQ=255)
                                         tttttGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCAtt                                                          <  2:2537607/100‑1 (MQ=255)
                                            ttgttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATc                                                       <  2:1407577/100‑1 (MQ=255)
                                            ttgttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATc                                                       <  2:934613/100‑1 (MQ=255)
                                             tgttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAACGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCa                                                      <  2:790169/100‑1 (MQ=255)
                                              gttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAg                                                     <  1:441715/100‑1 (MQ=255)
                                              gttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAg                                                     <  1:618149/100‑1 (MQ=255)
                                              gttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAg                                                     >  1:2323193/1‑100 (MQ=255)
                                              gttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAg                                                     <  1:2496209/100‑1 (MQ=255)
                                              gttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAg                                                     >  2:2403750/1‑100 (MQ=255)
                                                tgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGaa                                                   >  1:1449594/1‑100 (MQ=255)
                                                tgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGACGCATTATCAGaa                                                   >  1:955684/1‑100 (MQ=255)
                                                 gCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGaa                                                   >  1:2421419/1‑99 (MQ=255)
                                                   aTTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAttt                                                >  2:498383/1‑100 (MQ=255)
                                                    ttGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTg                                               <  2:659661/100‑1 (MQ=255)
                                                      gTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTa                                             <  1:1270769/100‑1 (MQ=255)
                                                      gTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTa                                             <  2:1267641/100‑1 (MQ=255)
                                                       tttCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAg                                            >  2:1052920/1‑100 (MQ=255)
                                                        ttCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAgg                                           <  1:1568463/100‑1 (MQ=255)
                                                           ctccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGa                                        >  2:562249/1‑100 (MQ=255)
                                                            tccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAt                                       >  1:1574371/1‑100 (MQ=255)
                                                               cAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATccc                                    >  1:1361097/1‑100 (MQ=255)
                                                                    aTTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAg                               <  2:904260/100‑1 (MQ=255)
                                                                    aTTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAg                               <  2:2364900/100‑1 (MQ=255)
                                                                     ttGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGa                              <  2:1692888/100‑1 (MQ=255)
                                                                      tGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaa                             >  1:668392/1‑100 (MQ=255)
                                                                       gCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaaa                            >  2:1621599/1‑100 (MQ=255)
                                                                       gCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaaa                            <  2:1361088/100‑1 (MQ=255)
                                                                       gCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaaa                            >  1:2010053/1‑100 (MQ=255)
                                                                          ccaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCa                         >  2:1607631/1‑100 (MQ=255)
                                                                           caccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAg                        <  1:364412/100‑1 (MQ=255)
                                                                           caccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAg                        <  1:874464/100‑1 (MQ=255)
                                                                            tttgttcataaaaaCATGGCGTGACTCCCTGTTTTTATGCTTAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAgg                       <  1:312372/90‑1 (MQ=255)
                                                                            accGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAgg                       <  1:1161574/100‑1 (MQ=255)
                                                                             ccGTAATGCAAAACAGGGCGTGACGCTCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                      <  2:755206/100‑1 (MQ=255)
                                                                             ccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                      >  1:2220055/1‑100 (MQ=255)
                                                                             ccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                      >  2:875822/1‑100 (MQ=255)
                                                                             ccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGc                      <  1:979054/100‑1 (MQ=255)
                                                                              cGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGcc                     <  1:1101172/100‑1 (MQ=255)
                                                                              cGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGcc                     <  1:419759/100‑1 (MQ=255)
                                                                               gTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCa                    >  1:2538511/1‑100 (MQ=255)
                                                                                tAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAg                   <  1:2349733/100‑1 (MQ=255)
                                                                                  cataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTc                 >  2:1254591/5‑100 (MQ=255)
                                                                                  cataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTc                 >  2:1785644/5‑100 (MQ=255)
                                                                                   tGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTcc                >  1:928435/1‑100 (MQ=255)
                                                                                   tGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTcc                >  2:1447765/1‑100 (MQ=255)
                                                                                     cAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAg              >  1:9481/1‑100 (MQ=255)
                                                                                     cAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAg              >  2:138260/1‑100 (MQ=255)
                                                                                     cAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATCTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAg              <  2:1038647/100‑1 (MQ=255)
                                                                                      aaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAgg             >  2:2254565/1‑100 (MQ=255)
                                                                                      aaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAgg             >  1:1289895/1‑100 (MQ=255)
                                                                                      aaaaCAGGGCATCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGACTTTGTAGGTGATCCCGGTAGAAGTCAGGCCAGTGCAgg             >  2:675729/1‑100 (MQ=255)
                                                                                       aaaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGa            >  1:2395952/1‑100 (MQ=255)
                                                                                       aaaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGa            >  2:1292325/1‑100 (MQ=255)
                                                                                       aaaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGa            >  2:2505061/1‑100 (MQ=255)
                                                                                        aaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAt           <  1:1318153/100‑1 (MQ=255)
                                                                                         aCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAtt          >  2:2402785/1‑100 (MQ=255)
                                                                                            gggCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTAt       <  2:344640/100‑1 (MQ=255)
                                                                                            gggCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTAt       >  1:95305/1‑100 (MQ=255)
                                                                                              gCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATcc     <  2:29243/100‑1 (MQ=255)
                                                                                                 tGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccacc  <  2:1673400/100‑1 (MQ=255)
                                                                                                  |                                                                                                  
CCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACC  >  NC_000913/1865584‑1865780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: