| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,880,851 | T→A | 16.3% | intergenic (‑92/+35) | leuE ← / ← dmlR | leucine efflux protein/transcriptional activator of dmlA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,880,851 | 0 | T | A | 16.3% | 181.1 / 27.7 | 79 | intergenic (‑92/+35) | leuE/dmlR | leucine efflux protein/transcriptional activator of dmlA |
| Reads supporting (aligned to +/- strand): ref base T (33/33); new base A (7/6); total (40/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.86e-01 | |||||||||||
GCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTAC > NC_000913/1880752‑1880948 | gCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGttt < 2:186883/100‑1 (MQ=255) gAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTa < 2:666333/100‑1 (MQ=255) cacaTTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAAcc < 1:2206345/100‑1 (MQ=255) tcaCCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGata < 1:2364296/98‑1 (MQ=255) tAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGatt < 1:499706/100‑2 (MQ=255) tAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGatt < 1:2379978/100‑2 (MQ=255) aaCCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGatat > 1:1275056/1‑100 (MQ=255) aaCCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGattt < 2:2502174/100‑3 (MQ=255) ctctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCaa > 2:512192/1‑100 (MQ=255) ctctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCaa < 2:2275991/100‑1 (MQ=255) ctctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGattttct < 1:594611/100‑6 (MQ=255) ctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAAcc < 1:1570351/100‑1 (MQ=255) ctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAAcc > 2:2320559/1‑100 (MQ=255) ctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATACTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGattttctcn < 2:829916/100‑8 (MQ=255) tttAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAAccc < 1:512198/100‑1 (MQ=255) ttAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGattttctcctt < 1:1822960/100‑10 (MQ=38) tAATTATCTTTCAATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGa < 2:1132550/100‑1 (MQ=255) aaTTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGAt > 1:1680019/1‑100 (MQ=255) aTTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATa < 1:1712282/100‑1 (MQ=255) tATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAAt < 1:1396603/100‑1 (MQ=255) tCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGAGATCAACCCGATAATTg < 2:2088393/100‑1 (MQ=255) tttCGATCATGCGCGATTAAAGGTGAATATGCTAACAAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTGTTAACCTGATATCAACCCGATAATTGaa < 2:2515516/100‑1 (MQ=255) ttCGATCATGCGCGATTAAAGGGGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGCGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAAt < 2:1400104/100‑1 (MQ=255) tCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAAtn < 1:640754/100‑2 (MQ=255) aTCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCAtt < 1:550939/100‑1 (MQ=255) tGCGCGATGAAAGGTGAATATGCTAAACAATCTGTCGCGGCTTAGAAAGGAGAAAATCAGTTTTTAATCTGATATCAACCCGATAATTGAATCATTAATc > 2:79206/1‑100 (MQ=255) cgggATTATAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCGGATAATTGAATCATTAATCa > 2:77093/4‑99 (MQ=255) cgcgATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAg > 1:894292/1‑100 (MQ=255) cgcgATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAg > 1:659960/1‑100 (MQ=255) nttAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCAt > 2:233862/2‑100 (MQ=255) ttAAAGGTGAATATGCTAACCAATCTATAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATg > 1:1562932/1‑100 (MQ=255) tAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGc > 1:1009279/1‑100 (MQ=255) aaGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTc > 2:1207967/1‑100 (MQ=255) ggTGAATATGCTAACCAATCTGTAGCGGCTTAGATAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTcc > 2:807368/1‑99 (MQ=255) gAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTg < 2:2348371/100‑1 (MQ=255) aaTATGCTAACCAATCTGTTGCGGCTTAAAAAGGAGAAAAGCAGGTTTTAACCTGGTATCAACCCGATAATTGAATCATTAATCAGGCATGATACAGTGa > 2:1322425/1‑100 (MQ=255) aaTATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGa < 1:2168667/100‑1 (MQ=255) tatGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGaaa < 1:2363797/100‑1 (MQ=255) atGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGaaaa < 1:1520401/100‑1 (MQ=255) aaCCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCg > 2:1103811/1‑100 (MQ=255) aCCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCgg < 1:1255918/100‑1 (MQ=255) aCCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCgg < 2:1381523/100‑1 (MQ=255) aCCAATCGGTACCGGCTTAGAAAGGAGAAAATCAAGTTTTAACCTGATATAAACCCGATAATTGAGTCATTAATCAGGCATGCTCCAGTGaaagcatcgg > 2:1342202/1‑93 (MQ=255) ccAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCggg < 1:1153289/100‑1 (MQ=255) ccAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCggg > 1:1283013/1‑100 (MQ=255) cAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCggg < 2:624932/99‑1 (MQ=255) tGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGc < 1:2381504/100‑1 (MQ=255) gTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCt > 1:1114127/1‑100 (MQ=255) cGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGc > 2:729314/1‑100 (MQ=255) ggCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCa > 1:763396/1‑100 (MQ=255) ggCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCa > 2:2509147/1‑100 (MQ=255) cTTAGAAAGGAGAAAATCAGGCTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCaaa < 2:142389/100‑1 (MQ=255) tAGGAAGGAGAAACTCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCCCGCCAAAAt > 2:1978550/1‑100 (MQ=255) tAGAAAGGGGACAGTCAGGAGTTAAACTGATATCAACCCGATAATTGAATCATTTATCAGGCATGCTCCAGAGAAAAATCCGATCACTGCTGGGCAAAAt > 2:569064/1‑100 (MQ=255) tAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAAt > 2:882173/1‑100 (MQ=255) gAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCAGGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATAc > 2:1391928/1‑100 (MQ=255) aaGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTg < 2:258502/100‑1 (MQ=255) aaGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTg < 1:2404555/100‑1 (MQ=255) aaGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGAGAAAAATTCGGGTAGTGCTCGGCAAAATACTg > 2:539639/1‑100 (MQ=255) aGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTgg < 1:434461/100‑1 (MQ=255) aGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTgg < 1:1225748/100‑1 (MQ=255) aGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTgg < 1:474143/100‑1 (MQ=255) ggttgataTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGc > 1:165591/8‑100 (MQ=255) ggttgataTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGc > 1:1399507/8‑100 (MQ=255) ggttgataTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGc > 2:2064806/8‑100 (MQ=255) ggAGCAAAACAGGGTTTACCCTGATATCAACCCGATCAGTGATTTATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATCCTgg < 2:2559623/99‑1 (MQ=255) ggAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTTCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGc > 2:1399887/1‑100 (MQ=255) ggAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGc > 1:1969584/1‑100 (MQ=255) nagaAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCg > 1:915420/2‑100 (MQ=255) ttgataTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAAGCATGCCCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGc > 2:771318/6‑98 (MQ=255) agaAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGt > 1:2038184/1‑100 (MQ=255) agaAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGATCCAGTGAAAAATTCGGGTAGCGCTCGGCAAAATACTGGc < 2:95967/98‑1 (MQ=255) tgataTCAGGTTAAAACCTGAGATCAACCCGATAATTGTATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGATAGTGCTCGGCAAAATACt > 2:2040656/5‑94 (MQ=255) gaAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTCATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTa > 1:1479602/1‑100 (MQ=255) ataTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTaaa > 1:1821453/3‑100 (MQ=255) ataTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTaaa > 1:1248740/3‑100 (MQ=255) aaTCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTaaaa < 2:2479047/100‑1 (MQ=255) aaTCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTaaaa > 2:1868758/1‑100 (MQ=255) aTCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTaaaaa < 1:2351568/100‑1 (MQ=255) ggTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCt > 1:1834962/1‑100 (MQ=255) ttttAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTAc < 2:546917/100‑1 (MQ=255) | GCGAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTAC > NC_000913/1880752‑1880948 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |