Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,880,852 T→A 16.1% intergenic (‑93/+34) leuE ← / ← dmlR leucine efflux protein/transcriptional activator of dmlA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,880,8520TA16.1% 191.7 / 25.2 79intergenic (‑93/+34)leuE/dmlRleucine efflux protein/transcriptional activator of dmlA
Reads supporting (aligned to +/- strand):  ref base T (33/33);  new base A (7/6);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

GAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGT  >  NC_000913/1880754‑1880951
                                                                                                  |                                                                                                   
gAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTa                                                                                                    <  2:666333/100‑1 (MQ=255)
   cacaTTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAAcc                                                                                                 <  1:2206345/100‑1 (MQ=255)
        tcaCCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGata                                                                                            <  1:2364296/98‑1 (MQ=255)
        tAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGatt                                                                                            <  1:2379978/100‑2 (MQ=255)
        tAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGatt                                                                                            <  1:499706/100‑2 (MQ=255)
         aaCCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGatat                                                                                           >  1:1275056/1‑100 (MQ=255)
         aaCCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGattt                                                                                           <  2:2502174/100‑3 (MQ=255)
            ctctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCaa                                                                                        >  2:512192/1‑100 (MQ=255)
            ctctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCaa                                                                                        <  2:2275991/100‑1 (MQ=255)
            ctctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGattttct                                                                                        <  1:594611/100‑6 (MQ=255)
              ctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAAcc                                                                                      <  1:1570351/100‑1 (MQ=255)
              ctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAAcc                                                                                      >  2:2320559/1‑100 (MQ=255)
              ctTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATACTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGattttctcn                                                                                      <  2:829916/100‑8 (MQ=255)
               tttAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAAccc                                                                                     <  1:512198/100‑1 (MQ=255)
                ttAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTAAAACCTGattttctcctt                                                                                    <  1:1822960/100‑10 (MQ=38)
                 tAATTATCTTTCAATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGa                                                                                   <  2:1132550/100‑1 (MQ=255)
                  aaTTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGAt                                                                                  >  1:1680019/1‑100 (MQ=255)
                   aTTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATa                                                                                 <  1:1712282/100‑1 (MQ=255)
                     tATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAAt                                                                               <  1:1396603/100‑1 (MQ=255)
                       tCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGAGATCAACCCGATAATTg                                                                             <  2:2088393/100‑1 (MQ=255)
                         tttCGATCATGCGCGATTAAAGGTGAATATGCTAACAAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTGTTAACCTGATATCAACCCGATAATTGaa                                                                           <  2:2515516/100‑1 (MQ=255)
                          ttCGATCATGCGCGATTAAAGGGGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGCGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAAt                                                                          <  2:1400104/100‑1 (MQ=255)
                           tCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAAtn                                                                         <  1:640754/100‑2 (MQ=255)
                              aTCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCAtt                                                                      <  1:550939/100‑1 (MQ=255)
                                  tGCGCGATGAAAGGTGAATATGCTAAACAATCTGTCGCGGCTTAGAAAGGAGAAAATCAGTTTTTAATCTGATATCAACCCGATAATTGAATCATTAATc                                                                  >  2:79206/1‑100 (MQ=255)
                                    cgggATTATAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCGGATAATTGAATCATTAATCa                                                                 >  2:77093/4‑99 (MQ=255)
                                    cgcgATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAg                                                                >  1:894292/1‑100 (MQ=255)
                                    cgcgATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAg                                                                >  1:659960/1‑100 (MQ=255)
                                        nttAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCAt                                                            >  2:233862/2‑100 (MQ=255)
                                         ttAAAGGTGAATATGCTAACCAATCTATAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATg                                                           >  1:1562932/1‑100 (MQ=255)
                                          tAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGc                                                          >  1:1009279/1‑100 (MQ=255)
                                            aaGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTc                                                        >  2:1207967/1‑100 (MQ=255)
                                              ggTGAATATGCTAACCAATCTGTAGCGGCTTAGATAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTcc                                                       >  2:807368/1‑99 (MQ=255)
                                                 gAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTg                                                   <  2:2348371/100‑1 (MQ=255)
                                                  aaTATGCTAACCAATCTGTTGCGGCTTAAAAAGGAGAAAAGCAGGTTTTAACCTGGTATCAACCCGATAATTGAATCATTAATCAGGCATGATACAGTGa                                                  >  2:1322425/1‑100 (MQ=255)
                                                  aaTATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGa                                                  <  1:2168667/100‑1 (MQ=255)
                                                    tatGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGaaa                                                <  1:2363797/100‑1 (MQ=255)
                                                     atGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGaaaa                                               <  1:1520401/100‑1 (MQ=255)
                                                          aaCCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCg                                          >  2:1103811/1‑100 (MQ=255)
                                                           aCCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCgg                                         <  2:1381523/100‑1 (MQ=255)
                                                           aCCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCgg                                         <  1:1255918/100‑1 (MQ=255)
                                                           aCCAATCGGTACCGGCTTAGAAAGGAGAAAATCAAGTTTTAACCTGATATAAACCCGATAATTGAGTCATTAATCAGGCATGCTCCAGTGaaagcatcgg                                         >  2:1342202/1‑93 (MQ=255)
                                                            ccAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCggg                                        <  1:1153289/100‑1 (MQ=255)
                                                            ccAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCggg                                        >  1:1283013/1‑100 (MQ=255)
                                                             cAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCggg                                        <  2:624932/99‑1 (MQ=255)
                                                                  tGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGc                                  <  1:2381504/100‑1 (MQ=255)
                                                                   gTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCt                                 >  1:1114127/1‑100 (MQ=255)
                                                                       cGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGc                             >  2:729314/1‑100 (MQ=255)
                                                                        ggCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCa                            >  2:2509147/1‑100 (MQ=255)
                                                                        ggCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCa                            >  1:763396/1‑100 (MQ=255)
                                                                          cTTAGAAAGGAGAAAATCAGGCTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCaaa                          <  2:142389/100‑1 (MQ=255)
                                                                            tAGGAAGGAGAAACTCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCCCGCCAAAAt                        >  2:1978550/1‑100 (MQ=255)
                                                                            tAGAAAGGGGACAGTCAGGAGTTAAACTGATATCAACCCGATAATTGAATCATTTATCAGGCATGCTCCAGAGAAAAATCCGATCACTGCTGGGCAAAAt                        >  2:569064/1‑100 (MQ=255)
                                                                            tAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAAt                        >  2:882173/1‑100 (MQ=255)
                                                                              gAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCAGGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATAc                      >  2:1391928/1‑100 (MQ=255)
                                                                                aaGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTg                    <  1:2404555/100‑1 (MQ=255)
                                                                                aaGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTg                    <  2:258502/100‑1 (MQ=255)
                                                                                aaGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGAGAAAAATTCGGGTAGTGCTCGGCAAAATACTg                    >  2:539639/1‑100 (MQ=255)
                                                                                 aGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTgg                   <  1:474143/100‑1 (MQ=255)
                                                                                 aGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTgg                   <  1:434461/100‑1 (MQ=255)
                                                                                 aGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTgg                   <  1:1225748/100‑1 (MQ=255)
                                                                                  ggttgataTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGc                  >  1:1399507/8‑100 (MQ=255)
                                                                                  ggttgataTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGc                  >  2:2064806/8‑100 (MQ=255)
                                                                                  ggttgataTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGc                  >  1:165591/8‑100 (MQ=255)
                                                                                  ggAGCAAAACAGGGTTTACCCTGATATCAACCCGATCAGTGATTTATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATCCTgg                   <  2:2559623/99‑1 (MQ=255)
                                                                                  ggAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTTCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGc                  >  2:1399887/1‑100 (MQ=255)
                                                                                  ggAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGc                  >  1:1969584/1‑100 (MQ=255)
                                                                                   nagaAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCg                 >  1:915420/2‑100 (MQ=255)
                                                                                    ttgataTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAAGCATGCCCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGc                  >  2:771318/6‑98 (MQ=255)
                                                                                    agaAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGt                >  1:2038184/1‑100 (MQ=255)
                                                                                    agaAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGATCCAGTGAAAAATTCGGGTAGCGCTCGGCAAAATACTGGc                  <  2:95967/98‑1 (MQ=255)
                                                                                     tgataTCAGGTTAAAACCTGAGATCAACCCGATAATTGTATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGATAGTGCTCGGCAAAATACt                     >  2:2040656/5‑94 (MQ=255)
                                                                                     gaAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTCATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTa               >  1:1479602/1‑100 (MQ=255)
                                                                                       ataTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTaaa             >  1:1821453/3‑100 (MQ=255)
                                                                                       ataTCAGGTTAAAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTaaa             >  1:1248740/3‑100 (MQ=255)
                                                                                        aaTCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTaaaa            <  2:2479047/100‑1 (MQ=255)
                                                                                        aaTCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTaaaa            >  2:1868758/1‑100 (MQ=255)
                                                                                         aTCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTaaaaa           <  1:2351568/100‑1 (MQ=255)
                                                                                             ggTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCt       >  1:1834962/1‑100 (MQ=255)
                                                                                               ttttAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTAc     <  2:546917/100‑1 (MQ=255)
                                                                                                  tAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGt  <  2:1680007/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GAACACATTAACCTCTTTAATTATCTTTCGATCATGCGCGATTAAAGGTGAATATGCTAACCAATCTGTAGCGGCTTAGAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATCATTAATCAGGCATGCTCCAGTGAAAAATTCGGGTAGTGCTCGGCAAAATACTGGCGTAAAAATTCTACCGT  >  NC_000913/1880754‑1880951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: