Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,971,761	T→C	100%	T311A (ACC→GCC)	tar ←	methyl‑accepting chemotaxis protein II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,971,761	0	T	C	100.0%	312.0 / ‑6.7	89	T311A (ACC→GCC)	tar	methyl‑accepting chemotaxis protein II
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (44/44);  minor base G (1/0);  total (45/44)

CCACCACTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGG  >  NC_000913/1971665‑1971856                                                                                                 |                                                                                                ccaccaCTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAg                                                                                              >  1:133661/1‑100 (MQ=255)  caccacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGGGAg                                                                                              >  2:1055499/1‑99 (MQ=255)  caccacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgc                                                                                             <  1:695947/100‑1 (MQ=255)   accacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgct                                                                                            <  1:922904/100‑1 (MQ=255)   accacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgct                                                                                            >  2:2178762/1‑100 (MQ=255)    ccacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgctg                                                                                           >  1:105942/1‑100 (MQ=255)    ccacTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAgctg                                                                                           >  1:1476245/1‑100 (MQ=255)        tttGGCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTcc                                                                                       <  2:1261601/100‑1 (MQ=255)          tGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGGGCTGCTcc                                                                                       >  2:1470359/1‑98 (MQ=255)          tGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCAt                                                                                     >  1:2358994/1‑100 (MQ=255)           gccgccGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATg                                                                                    <  2:98791/100‑1 (MQ=255)            cggccgTGGTGGGCGGTGTCGGAGGCACTGTGCGCCAGTTGCGAGGTCTGGCGGGCGTTATCGGCGTTTTGCGTCACTGTCGCGGCGAGCTGCTCCATGc                                                                                   <  2:2317400/98‑1 (MQ=255)            ccgccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGc                                                                                   >  1:439843/1‑100 (MQ=255)            ccgccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGc                                                                                   >  2:1596164/1‑100 (MQ=255)             cgccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCt                                                                                  >  2:1987182/1‑100 (MQ=255)              gccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTg                                                                                 >  2:986424/1‑100 (MQ=255)               ccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTgg                                                                                <  1:2192224/100‑1 (MQ=255)               ccgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTgg                                                                                <  1:2190609/100‑1 (MQ=255)                cgTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTggc                                                                               <  1:1483727/100‑1 (MQ=255)                   gatGGGCGGTGTCAGAGGCACTTTGCGCCAGATGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTggcggc                                                                            >  1:1727713/3‑100 (MQ=255)                   gCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTggcggc                                                                            >  2:1600494/1‑100 (MQ=255)                     tGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAg                                                                          <  2:505896/100‑1 (MQ=255)                      gggCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGt                                                                         >  1:2392658/1‑100 (MQ=255)                       gtcGGTGTCGTAGTCATTTTGCTCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTCTTGCTTCACTGTCGCGGCGAGCTTCTCCATGCTggcggc                                                                            <  2:133637/94‑1 (MQ=255)                        gCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGttt                                                                       >  1:1452691/1‑100 (MQ=255)                        gCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGttt                                                                       <  2:982807/100‑1 (MQ=255)                           gtgtCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTttctt                                                                    >  1:893540/1‑100 (MQ=255)                             gtCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTcc                                                                  <  1:1648440/100‑1 (MQ=255)                              tCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCa                                                                 >  2:105234/1‑100 (MQ=255)                                ggAGGCACTTTGCGCCAGTTGCTAGGCCTGGTGGGCGCTATCGGCGTCTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAgc                                                               <  2:1729175/100‑1 (MQ=255)                                ggAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAgc                                                               >  1:718673/1‑100 (MQ=255)                                ggAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAgc                                                               <  2:2392644/100‑1 (MQ=255)                                 gAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAgcg                                                              >  2:2351732/1‑100 (MQ=255)                                   ggCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGcgcg                                                            >  2:1998633/1‑100 (MQ=255)                                   ggCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGcgcg                                                            >  2:1308606/1‑100 (MQ=255)                                    gCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCgg                                                           <  1:1355055/100‑1 (MQ=255)                                     cACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGa                                                          <  2:1601659/100‑1 (MQ=255)                                     cACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGa                                                          >  1:1253000/1‑100 (MQ=255)                                      aCTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGAt                                                         >  1:2015356/1‑100 (MQ=255)                                         ttGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGcc                                                      <  1:2351747/100‑1 (MQ=255)                                            cgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgc                                                   >  1:395405/1‑100 (MQ=255)                                            cgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgc                                                   <  1:412111/100‑1 (MQ=255)                                            cgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgc                                                   <  1:2378829/100‑1 (MQ=255)                                            cgcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgc                                                   >  1:2155457/1‑100 (MQ=255)                                             gcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgct                                                  >  2:1576674/1‑100 (MQ=255)                                             gcCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgc                                                   <  2:999909/99‑1 (MQ=255)                                              ccAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgctg                                                 <  1:105236/100‑1 (MQ=255)                                              ccAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgctg                                                 <  2:937131/100‑1 (MQ=255)                                              ccAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCctgctg                                                 <  1:901679/100‑1 (MQ=255)                                                aGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGt                                                >  2:2331439/1‑99 (MQ=255)                                                 gTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTc                                              <  2:2015342/100‑1 (MQ=255)                                                   tGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAg                                            >  1:487316/1‑100 (MQ=255)                                                   tGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAg                                            <  1:1316768/100‑1 (MQ=255)                                                   tGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAg                                            <  1:2224660/100‑1 (MQ=255)                                                    gCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAg                                            <  2:1177634/99‑1 (MQ=255)                                                    gCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGt                                           >  1:1697448/1‑100 (MQ=255)                                                     cGAGGCCTGTCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAg                                            >  2:1597985/1‑98 (MQ=255)                                                        ggCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACgg                                       >  1:1496255/1‑100 (MQ=255)                                                          ccTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGgga                                     >  1:928080/1‑100 (MQ=255)                                                          ccTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTATTCCAGCGCGGATGCCTGCTGTTCAGTACGgga                                     >  2:1256199/1‑100 (MQ=255)                                                            tGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGggagg                                   <  2:1308592/100‑1 (MQ=255)                                                             ggcgggcgTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGACGCCTGCTGTTCAGTACGggagga                                  >  1:2220007/1‑100 (MQ=255)                                                                  gcgTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGAt                             >  1:1540471/1‑100 (MQ=255)                                                                   cgTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATc                            <  1:51012/100‑1 (MQ=255)                                                                   cgTTATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATc                            <  1:890576/100‑1 (MQ=255)                                                                     ttATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCgg                          <  1:2557879/100‑1 (MQ=255)                                                                      tATCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCCGCTGTTCAGTACGGGAGGAAAGATCGgt                         <  1:838094/100‑1 (MQ=255)                                                                        tCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGgtgt                       >  1:2075502/1‑100 (MQ=255)                                                                        tCGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGgtgt                       <  2:475730/100‑1 (MQ=255)                                                                         cGGCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGtt                      >  1:1921086/1‑100 (MQ=255)                                                                           gCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGc                    <  1:1600505/100‑1 (MQ=255)                                                                           gCGTTTTGCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGc                    >  2:2536787/1‑100 (MQ=255)                                                                                  gCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCcgccgc             >  1:1633545/1‑100 (MQ=255)                                                                                  gCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCcgccgc             <  1:1822236/100‑1 (MQ=255)                                                                                  gCTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCcgccgc             >  2:2522988/1‑100 (MQ=255)                                                                                   cTTCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCa            >  1:1238809/1‑100 (MQ=255)                                                                                     tCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAAt          >  1:1414107/1‑100 (MQ=255)                                                                                     tCACTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGACGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAAt          <  2:2219993/100‑1 (MQ=255)                                                                                       aCTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAAttt        >  2:833400/1‑100 (MQ=255)                                                                                        cTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTc       <  2:1892701/100‑1 (MQ=255)                                                                                        cTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTc       <  1:1799149/100‑1 (MQ=255)                                                                                        cTGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTc       >  2:2055477/1‑100 (MQ=255)                                                                                         tGTCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCa      <  2:491317/100‑1 (MQ=255)                                                                                           tCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACg    <  2:365779/100‑1 (MQ=255)                                                                                           tCGCGGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACg    <  1:895605/100‑1 (MQ=255)                                                                                            cgcgGCGTGCTGCTCCATGCTGGCGGCAGGTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACg    >  2:1425474/1‑99 (MQ=255)                                                                                            cgcgGCGAGCTGCTCCATGCTGGCGTCAGTTTCTTCCGGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACgg   <  1:1386717/100‑1 (MQ=255)                                                                                            cgcgGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACgg   <  2:85023/100‑1 (MQ=255)                                                                                            cgcgGCGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACgg   <  2:602652/100‑1 (MQ=255)                                                                                             gcggcgAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACggg  >  2:145561/1‑100 (MQ=255)                                                                                                 |                                                                                                CCACCACTTTGCCGCCGTGCTGGGCGGTGTCGGAGGCACTTTGCGCCAGTTGCGAGGCCTGGCGGGCGTTATCGGCGTTTTGCTTCACTGTCGCGGTGAGCTGCTCCATGCTGGCGGCAGTTTCTTCCAGCGCGGATGCCTGCTGTTCAGTACGGGAGGAAAGATCGGTGTTGCCCGCCGCAATTTCACGGG  >  NC_000913/1971665‑1971856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑