Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,983,761	C→T	100%	E437K (GAA→AAA)	araG ←	L‑arabinose ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,983,761	0	C	T	100.0%	280.3 / ‑6.7	82	E437K (GAA→AAA)	araG	L‑arabinose ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (39/42);  minor base G (0/1);  total (39/43)

TCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCT  >  NC_000913/1983665‑1983856                                                                                                 |                                                                                                tCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACATCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTtgtg                                                                                              <  2:2399997/100‑1 (MQ=255)  ccGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGc                                                                                             >  2:408908/1‑100 (MQ=255)  ccGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGc                                                                                             <  2:1035744/100‑1 (MQ=255)    ggTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCtt                                                                                           >  2:1281664/1‑100 (MQ=255)     gTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTa                                                                                          <  1:742350/100‑1 (MQ=255)       cGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAg                                                                                         <  2:280983/99‑1 (MQ=255)        ggCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAgcg                                                                                       >  1:899048/1‑100 (MQ=255)         gCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAgcgc                                                                                      >  1:850392/1‑100 (MQ=255)         gCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAgcgc                                                                                      >  1:1413700/1‑100 (MQ=255)         gCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAgcgc                                                                                      >  1:1465628/1‑100 (MQ=255)          cAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGcc                                                                                     <  2:1392896/100‑1 (MQ=255)             cGCCGAGGACTTCAGGTAAGTTGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAAc                                                                                  <  1:1057386/100‑1 (MQ=255)             cGCCGAGGACTTCAGGTAAGTTGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAAc                                                                                  <  1:1244675/100‑1 (MQ=255)             cGCCGAGGACTTCAGGTAAGTCGCTGGAGGTAAACACCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCaa                                                                                   <  2:1728075/99‑1 (MQ=255)             cGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAAc                                                                                  <  2:1717054/100‑1 (MQ=255)              tccGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACa                                                                                 <  1:378622/99‑1 (MQ=255)              gCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACa                                                                                 <  1:896365/100‑1 (MQ=255)               ccGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACaa                                                                                >  2:378618/1‑99 (MQ=255)                 gAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCa                                                                              >  1:288642/1‑100 (MQ=255)                  aGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCaa                                                                             <  1:1119933/100‑1 (MQ=255)                     aCTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGc                                                                          >  1:305827/1‑100 (MQ=255)                          aGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgcgc                                                                     <  1:602021/100‑1 (MQ=255)                           ggTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCcgcgcg                                                                    >  1:309388/1‑100 (MQ=255)                             tAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTa                                                                  <  1:448581/100‑1 (MQ=255)                             tAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTa                                                                  <  2:303878/100‑1 (MQ=255)                               aGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAgg                                                                >  1:843409/1‑100 (MQ=255)                               aGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAgg                                                                <  2:1449361/100‑1 (MQ=255)                                gTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGt                                                               <  2:185136/100‑1 (MQ=255)                                 tCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATCTACTTTATGCTTAGCGCCAACATCAATGCCGCGCGTAgg                                                                >  2:39257/1‑98 (MQ=255)                                 tCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGtt                                                              <  2:954026/100‑1 (MQ=255)                                   gCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCa                                                            >  1:1830097/1‑100 (MQ=255)                                   gCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCa                                                            <  1:1817337/100‑1 (MQ=255)                                      ggcggCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATcc                                                         >  1:31967/4‑100 (MQ=255)                                       gAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCa                                                        >  2:832552/1‑100 (MQ=255)                                        aGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAg                                                       >  1:1593733/1‑100 (MQ=255)                                         ggCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGc                                                      >  1:347190/1‑100 (MQ=255)                                          gCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCa                                                     >  1:1100659/1‑100 (MQ=255)                                          gCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCa                                                     >  2:718589/1‑100 (MQ=255)                                           cAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaa                                                    >  1:1983276/1‑100 (MQ=255)                                           cAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaa                                                    >  1:804781/1‑100 (MQ=255)                                           cAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaa                                                    >  2:537967/1‑100 (MQ=255)                                           cAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCaa                                                    >  2:1508653/1‑100 (MQ=255)                                               cAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATg                                                <  2:2264477/100‑1 (MQ=255)                                               cAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATg                                                >  1:11692/1‑100 (MQ=255)                                                aGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGa                                               >  1:1206993/1‑100 (MQ=255)                                                  cACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGAcc                                             <  1:2496897/100‑1 (MQ=255)                                                  cACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGAcc                                             <  1:648643/100‑1 (MQ=255)                                                   ttccgatctcccTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCt                                            <  1:383724/91‑1 (MQ=255)                                                    ccGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCtt                                           <  1:993785/100‑1 (MQ=255)                                                      gCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCa                                         >  1:2274768/1‑100 (MQ=255)                                                            ttatGCGCTGGCGGCGCATAAATTACGTTATATATTTTGTGCTTAGGGCTAACATCAATGTCACGAGAAGGTTCGTCGCGCACAATGACCTTCAt                                        >  2:25922/4‑95 (MQ=255)                                                            cccTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCtagatcggaa                                   >  2:383720/1‑91 (MQ=255)                                                            cccTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCtt                                   >  1:779624/1‑100 (MQ=255)                                                             ccTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTc                                  >  2:1412807/1‑100 (MQ=255)                                                             ccTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTc                                  >  1:631725/1‑100 (MQ=255)                                                              cTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTcc                                 <  1:671727/100‑1 (MQ=255)                                                              cTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTcc                                 <  2:1792177/100‑1 (MQ=255)                                                              cTGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTcc                                 <  2:850385/100‑1 (MQ=255)                                                               tGCGCCGCCAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTcc                                 <  2:2373943/99‑1 (MQ=255)                                                                    cgccAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAAc                           <  1:2379290/100‑1 (MQ=255)                                                                    cgccAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAAc                           >  2:514042/1‑100 (MQ=255)                                                                    cgccAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAAc                           <  1:144804/100‑1 (MQ=255)                                                                     gccAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAAcc                          <  1:1452038/100‑1 (MQ=255)                                                                       cAGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAg                        <  1:1254207/100‑1 (MQ=255)                                                                        aGCGCATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGc                       >  1:1043020/1‑100 (MQ=255)                                                                         tctcATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCTATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCg                      <  2:1634305/97‑1 (MQ=255)                                                                           gcATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGc                    <  1:353210/100‑1 (MQ=255)                                                                           gcATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGc                    <  1:1138417/100‑1 (MQ=255)                                                                            cATAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCgaga                   >  1:1634317/1‑97 (MQ=255)                                                                             aTAAATTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTATGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGccc                  >  2:2104663/1‑100 (MQ=255)                                                                               aaaTTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAg                <  2:276664/100‑1 (MQ=255)                                                                               aaaTTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAg                <  2:276679/100‑1 (MQ=255)                                                                                 aTTACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGaa              >  2:1668193/1‑100 (MQ=255)                                                                                  ttACGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAAt             <  2:265273/100‑1 (MQ=255)                                                                                    aCGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATgn           <  2:2322106/100‑2 (MQ=255)                                                                                    aCGTTATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATgg           <  2:355637/100‑1 (MQ=255)                                                                                       ttATATATTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCtt        <  2:597606/100‑1 (MQ=255)                                                                                        tatataTTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCttt       >  1:2100300/1‑100 (MQ=255)                                                                                            tatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGc   <  2:2072216/100‑1 (MQ=255)                                                                                            tatTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGc   <  1:2208889/100‑1 (MQ=255)                                                                                            tatTTGGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAACTGACCTTCATCTCCTCCGATAACCAGCGGCCCAGAATGGCTTTTTGc   <  2:2426896/100‑1 (MQ=255)                                                                                             atTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCt  >  1:175212/1‑100 (MQ=255)                                                                                             atTTTGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCt  >  2:2187785/1‑100 (MQ=255)                                                                                                 |                                                                                                TCCGGTCGGCAACGCCGAGGACTTCAGGTAAGTCGCTGGAGGCAAACAGCACCGCCACGCCCTGCGCCGCCAGCGCATAAATTACGTTATATATTTCGTGCTTAGCGCCAACATCAATGCCGCGCGTAGGTTCATCCAGCAAAATGACCTTCATCTCTTCCGATAACCAGCGGCCCAGAATGGCTTTTTGCT  >  NC_000913/1983665‑1983856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑