Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,089,854 A→T 17.4% intergenic (‑141/‑142) yefM ← / → hisL antitoxin of the YoeB‑YefM toxin‑antitoxin system/his operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,089,8540AT17.4% 258.1 / 42.3 108intergenic (‑141/‑142)yefM/hisLantitoxin of the YoeB‑YefM toxin‑antitoxin system/his operon leader peptide
Reads supporting (aligned to +/- strand):  ref base A (46/43);  new base T (8/11);  total (54/54)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

Only 100 of 109 total aligned reads displayed.

GTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTA  >  NC_000913/2089756‑2089953
                                                                                                  |                                                                                                   
gTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAg                                                                                                    <  2:2487063/100‑1 (MQ=255)
      taaCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCctct                                                                                              <  2:982927/100‑1 (MQ=255)
      taaCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCctct                                                                                              >  1:811248/1‑100 (MQ=255)
       aaCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCtt                                                                                             >  1:114371/1‑100 (MQ=255)
        aCTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTa                                                                                            >  2:1081912/1‑100 (MQ=255)
         cTATTTATTAATTAGTTTGTAGATCAAGGTATTGTGAGTGAGACGAAAATCTAGACTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCATCTTa                                                                                            >  2:1821585/1‑99 (MQ=255)
          tatttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGGGCCGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGc                                                                                          >  1:1208507/1‑100 (MQ=255)
          tatttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGCCACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACTGTCCTCTTAGc                                                                                          <  2:2487314/100‑1 (MQ=255)
          tatttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACTGTCCTCTTAGc                                                                                          <  1:688938/100‑1 (MQ=255)
          tatttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACTGTCCTCTTAGc                                                                                          <  1:1519575/100‑1 (MQ=255)
          tatttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACTGTCCTCTTAGc                                                                                          <  1:1521178/100‑1 (MQ=255)
          tatttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGc                                                                                          >  1:39801/1‑100 (MQ=255)
           atttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACTGTCCTCTTAGct                                                                                         <  1:1636508/100‑2 (MQ=255)
           atttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGcc                                                                                         <  1:1998/100‑1 (MQ=255)
           atttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGcc                                                                                         <  1:1960896/100‑1 (MQ=255)
            tttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACTGTCCTCTTAGctg                                                                                        <  1:315618/100‑3 (MQ=255)
            tttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACTGTCCTCTTAGctg                                                                                        <  1:905626/100‑3 (MQ=255)
            tttattAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACTGTCCTCTCAGctg                                                                                        <  1:1299985/100‑3 (MQ=255)
             ttattaATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGcccc                                                                                       <  1:1282631/100‑1 (MQ=255)
              tattaATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACTGTCCTCTTAGctgat                                                                                      <  1:1297010/100‑5 (MQ=255)
              tattaATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACTGTCCTCTTAGctgat                                                                                      <  1:1117130/100‑5 (MQ=255)
              tattaATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGccccc                                                                                      >  2:1543825/1‑100 (MQ=255)
              tattaATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGccccc                                                                                      <  1:641921/100‑1 (MQ=255)
              atctaattaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACTGTCCTCTTAGctgat                                                                                      <  1:1055969/97‑5 (MQ=39)
               attaattaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCcct                                                                                     >  2:1110155/1‑100 (MQ=255)
               attaattaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCcct                                                                                     >  1:1949531/1‑100 (MQ=255)
                ttaattaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCcctc                                                                                    <  1:1634859/100‑1 (MQ=255)
                 taattaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCcctcc                                                                                   >  1:1993645/1‑100 (MQ=255)
                  aattaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACTGTCCTCTTAGctgatagat                                                                                  >  2:1055961/1‑92 (MQ=39)
                   attaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCtt                                                                                 >  1:595006/1‑100 (MQ=255)
                   attaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCtt                                                                                 >  2:475497/1‑100 (MQ=255)
                   attaGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCtt                                                                                 >  1:1823351/1‑100 (MQ=255)
                      aGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTcc                                                                              >  1:1219433/1‑100 (MQ=255)
                      aGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTcc                                                                              <  2:1823337/100‑1 (MQ=255)
                      aGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTcc                                                                              >  1:786730/1‑100 (MQ=255)
                           gTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCt                                                                         <  1:2098945/100‑1 (MQ=255)
                           gTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCt                                                                         <  1:2552809/100‑1 (MQ=255)
                           gTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCt                                                                         <  1:1677496/100‑1 (MQ=255)
                             aGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTTTTGTTTTTTTCAACGAGGACAGTCCTATTAGCTCCCTCCTTTc                                                                               >  2:1692439/1‑92 (MQ=255)
                             aGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtca                                                                       <  1:1375443/100‑1 (MQ=255)
                              gATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtcat                                                                      >  1:1090877/1‑100 (MQ=255)
                              gATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtcat                                                                      >  1:1306372/1‑100 (MQ=255)
                              gATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtcat                                                                      >  2:1973600/1‑100 (MQ=255)
                              gATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtcat                                                                      >  1:1806215/1‑100 (MQ=255)
                                tCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCtcattc                                                                    >  1:1575663/1‑100 (MQ=255)
                                    ggTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTa                                                                <  1:20946/100‑1 (MQ=255)
                                       aTTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAAc                                                             <  1:443178/100‑1 (MQ=255)
                                       aTTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAAc                                                             >  2:977650/1‑100 (MQ=255)
                                              gTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCa                                                      >  2:2040049/1‑100 (MQ=255)
                                                  gaCGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGc                                                  >  2:815254/1‑100 (MQ=255)
                                                  gaCGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGc                                                  >  1:1421644/1‑100 (MQ=255)
                                                  gaCGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGc                                                  <  2:1993631/100‑1 (MQ=255)
                                                  gaCGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCGCCTTTCCCCGCTCATTCATTAAACAAATCCATTGc                                                  <  2:1353928/100‑1 (MQ=255)
                                                  gaCGAAAAGCCAGGCTTCGCTATTTTTGCTGCCATCAGCTAAGAGGACAGTCCTCTCAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGc                                                  <  2:1557046/100‑1 (MQ=255)
                                                     gAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCAt                                               <  1:1543836/100‑1 (MQ=255)
                                                         aTCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCACGCTCATTCATTAAACAAATCCATTGCCATaaaa                                           >  2:1352259/1‑100 (MQ=255)
                                                          tCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAat                                          >  1:28458/1‑100 (MQ=255)
                                                           ccAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCAATTGCCACAAAata                                         >  2:701709/1‑100 (MQ=255)
                                                             aGGCTTCGCTATTTTTGGTGCTATCAGCTAAGAGGACTGTCCTCTTAGCCCCCTCCTTTCCCCGCCCATTCATTAAACAAATCCATTGCCATAACatct                                        >  2:1498101/1‑97 (MQ=255)
                                                             aGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAatata                                       <  1:1074899/100‑1 (MQ=255)
                                                               gCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAAtatata                                     <  1:424332/100‑1 (MQ=255)
                                                                 ttCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATaaa                                   <  1:1744357/100‑1 (MQ=255)
                                                                  tCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCTGCTCATTCATTAAACAAATCCATTGCCATAAAATATATaaaa                                  >  2:1574374/1‑100 (MQ=255)
                                                                    gCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATaaaaaa                                >  1:2140615/1‑100 (MQ=255)
                                                                    gCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATaaaaaa                                >  2:2198421/1‑100 (MQ=255)
                                                                     cTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAg                               >  1:2455888/1‑100 (MQ=255)
                                                                     cTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAg                               <  1:7084/100‑1 (MQ=255)
                                                                      tATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGc                              >  1:205104/1‑100 (MQ=255)
                                                                       tcttttGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGcc                             <  1:447483/98‑1 (MQ=255)
                                                                       aTTTTTGGTGCCATCAGCTAAGAGGACAGTTCTCTGAGCCCCGTCCTTTCCCAGCACATTCATTAAACAGATCCATTGCCATAAAATATATAAAAAAgn                              <  2:117571/99‑2 (MQ=255)
                                                                         ttttGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCt                           >  2:2312791/1‑100 (MQ=255)
                                                                          tttGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGccaga                          >  2:447479/1‑97 (MQ=255)
                                                                            tGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGc                        >  2:1455646/1‑100 (MQ=255)
                                                                               tGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCttt                     <  2:2194167/100‑1 (MQ=255)
                                                                                gCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTc                    <  1:917527/100‑1 (MQ=255)
                                                                                 ccATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCt                   >  2:1793231/1‑100 (MQ=255)
                                                                                  cATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTa                  <  2:1949517/100‑1 (MQ=255)
                                                                                   aTCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTaa                 <  1:731005/100‑1 (MQ=255)
                                                                                     gggCTAAGAGGACTGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACg               >  2:528525/3‑100 (MQ=255)
                                                                                     cAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACg               <  1:7294/100‑1 (MQ=255)
                                                                                      ggCTAAGAGGACTGTCCTCTTAGCTCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGt              >  2:859643/2‑100 (MQ=255)
                                                                                      aGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACg               >  2:2509509/1‑99 (MQ=255)
                                                                                      aGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGt              >  1:1907212/1‑100 (MQ=255)
                                                                                       gCTAAGAGGACTGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTg             >  1:2339678/1‑100 (MQ=255)
                                                                                       gCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTg             >  1:29664/1‑100 (MQ=255)
                                                                                       gCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTg             >  1:438296/1‑100 (MQ=255)
                                                                                       gCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTACCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTg             <  2:90597/100‑1 (MQ=255)
                                                                                       gCTAAGAGGACAGTCCTCGTAGACCCCTCCTTTCCCAGCTCATTCATTAAACAAATCAATTGCCATAAAATATATAAAAAAGCCCTTGCTGTCTAATGt              >  2:663651/1‑99 (MQ=255)
                                                                                        cTAAGAGGACTGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTGa            >  2:1452359/1‑100 (MQ=255)
                                                                                        cTAAGAGGACTGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTGa            >  2:1262492/1‑100 (MQ=255)
                                                                                         tAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTGaa           <  2:2011254/100‑1 (MQ=255)
                                                                                         tAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTGaa           <  2:1635725/100‑1 (MQ=255)
                                                                                         tAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTGa            >  2:2286734/1‑99 (MQ=255)
                                                                                          aaGAGGACTGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTGaaa          >  1:399197/1‑100 (MQ=255)
                                                                                          aaGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTGtaa          >  2:967117/1‑100 (MQ=255)
                                                                                           agagGACAGACCTCTTCGCCCCCTCCTTACACCGCTCATTCATTAAACAAATCCATTTCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTGAAAg         <  2:2274395/100‑1 (MQ=255)
                                                                                              ggACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTgg      <  2:696595/100‑1 (MQ=255)
                                                                                               gACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCACTTGCTTTCTAACGTGAAAGTGGt     <  2:65535/100‑1 (MQ=255)
                                                                                                 cAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGttt   <  2:2380997/100‑1 (MQ=255)
                                                                                                  aGTCCTCTTAGCACCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTa  <  1:2185554/100‑1 (MQ=255)
                                                                                                  |                                                                                                   
GTTAATTAACTATTTATTAATTAGTTTGTAGATCAAGGTATTGTCAGTGAGACGAAAATCCAGGCTTCGCTATTTTTGGTGCCATCAGCTAAGAGGACAGTCCTCTTAGCCCCCTCCTTTCCCCGCTCATTCATTAAACAAATCCATTGCCATAAAATATATAAAAAAGCCCTTGCTTTCTAACGTGAAAGTGGTTTA  >  NC_000913/2089756‑2089953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: