| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,250,210 | T→A | 100% | V165E (GTG→GAG) | yeiH → | UPF0324 family inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,250,210 | 0 | T | A | 95.4% | 242.4 / 1.2 | 80 | V165E (GTG→GAG) | yeiH | UPF0324 family inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (1/3); new base A (36/39); total (37/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTT > NC_000913/2250117‑2250306 | gCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGACGCTg > 2:1109690/1‑99 (MQ=255) atcttGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTg < 2:1701567/97‑1 (MQ=255) ggTTGATCGTTGGCGGTAGCACTAGCTGTGTTGCTGCCGCGGTACTGGTGACTAAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTg < 2:1377162/96‑1 (MQ=255) gTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCa > 2:996949/1‑100 (MQ=255) gTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCa > 2:1827081/1‑100 (MQ=255) tGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTgagat > 1:1701579/1‑96 (MQ=255) gATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAAcc > 2:1465239/1‑100 (MQ=255) aTCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCg > 1:1528143/1‑100 (MQ=255) tCGGGGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGTCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCgt < 2:1706546/100‑1 (MQ=255) ggTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCgttg < 1:1840651/100‑1 (MQ=255) ggTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCgttg < 1:422430/100‑1 (MQ=255) gTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCgttgt < 1:672363/100‑1 (MQ=255) gTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCgttgt < 2:1754907/100‑1 (MQ=255) gTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCgttgt < 1:2076999/100‑1 (MQ=255) gTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCgttgt < 1:972417/100‑1 (MQ=255) tGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCgttgtt > 2:623258/1‑100 (MQ=255) tGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCgttgtt < 2:2517489/100‑1 (MQ=255) gCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTa < 1:858635/100‑1 (MQ=255) gCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTa > 2:1760500/1‑100 (MQ=255) gCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTa < 1:2280790/100‑1 (MQ=255) aGCAGAATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCg > 1:1777383/1‑100 (MQ=255) cAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCggg > 1:2489291/1‑100 (MQ=255) gtggtgCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTcgc < 1:13212/100‑1 (MQ=255) tggtgCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCTGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTcgcg < 1:1688489/100‑1 (MQ=255) gtgCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGAt < 1:73627/100‑1 (MQ=255) gtgCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGAt < 1:2475565/100‑1 (MQ=255) tgctgcCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGAtt > 1:1676876/1‑100 (MQ=255) gcCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTcc < 1:2353928/100‑1 (MQ=255) gcCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTcc < 1:2099608/100‑1 (MQ=255) gcCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTcc > 2:1937841/1‑100 (MQ=255) cgcgGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAGGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCctc < 2:2493832/100‑1 (MQ=255) cgcgGTACTGGCCACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCctc > 1:1681479/1‑100 (MQ=255) gTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTAcc > 1:120838/1‑100 (MQ=255) gTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTAcc > 1:120858/1‑100 (MQ=255) tACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTAccc < 1:1220366/100‑1 (MQ=255) cTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCgg > 1:1026266/1‑100 (MQ=255) cTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCgg < 2:698626/100‑1 (MQ=255) ggCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCg < 2:456561/100‑1 (MQ=255) ggCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCg < 1:730188/100‑1 (MQ=255) ggCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCg > 1:2059063/1‑100 (MQ=255) ggCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTCCGGGACCGTCGCGATTTTCCTCTACCCcgcg > 2:735651/1‑100 (MQ=255) cGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGat < 1:550260/100‑1 (MQ=255) cGACTGAGCCCGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTCCGTGACCGTCGCGATTTTCCTCTACCCGGCGat < 2:1681467/100‑1 (MQ=255) gACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGata < 1:1959474/100‑1 (MQ=255) gACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGata > 2:1789819/1‑100 (MQ=255) cTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGatata > 2:507537/1‑100 (MQ=255) cTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGatata > 2:2449388/1‑100 (MQ=255) cTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAACTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGatata < 2:1798571/100‑1 (MQ=255) aGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATcc > 1:823401/1‑100 (MQ=255) gCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCg > 1:2358219/1‑100 (MQ=255) gCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCg > 1:1668039/1‑100 (MQ=255) cggtgaGTTAAGGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGGTATATTCGGGACCTTCGCGATTTTCCTTTACCCGGCGATATATCCg < 2:107371/94‑1 (MQ=255) ccGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGc < 1:2449402/100‑1 (MQ=255) ccGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCACGATTTTCCTCTACCCGGCGATATATCCGc < 1:1410179/100‑1 (MQ=255) gTAGTGAAAGCGGAAGCCAGTAAAGAAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGa < 2:2107560/100‑1 (MQ=255) tAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCt < 2:447965/97‑1 (MQ=255) gTGAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGt < 2:1528132/100‑1 (MQ=255) gAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtct > 2:594072/1‑100 (MQ=255) gAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtct > 2:1031142/1‑100 (MQ=255) gAAAGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtct > 2:2207514/1‑100 (MQ=255) aaaGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtctc > 2:134349/1‑100 (MQ=255) aaaGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGtctc < 2:2512760/100‑1 (MQ=255) aGCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCaa > 2:1547890/1‑100 (MQ=255) gCGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAAt > 2:869906/1‑100 (MQ=255) cGGAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATg > 2:1332180/1‑100 (MQ=255) gaaaGCCAGTACATTAACCGAAGCTGTTGCAACCGTAGTTATCGTCAGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATgg < 2:1517/98‑1 (MQ=255) gaatcgcggaaagtgaTCGGGGCTGTTTCAACGCTTGTTATTTTCGGGACCGGCGCGGTTTTCCTCTGCCCGGCGATATATCCGCTGATGTCTCACTgg < 2:1503/90‑1 (MQ=255) gAAGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGt > 2:123553/1‑100 (MQ=255) aaGCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGtt < 2:1664738/100‑1 (MQ=255) gCCAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGttt < 1:1833658/99‑1 (MQ=255) ccAGTAAAGTAACCGAGGCTGTTGCAACCGTTGTTAGCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAg < 2:1413094/100‑1 (MQ=255) gtaaagtaaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTcc > 1:1257174/1‑100 (MQ=255) gtaaagtaaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTcc < 1:1357824/100‑1 (MQ=255) aaagtaaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCgg < 1:875040/100‑1 (MQ=255) aaagtaaCCGAGGCTGTTGCAACCGTTGTTATATTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATCTCCGCTGATGTCTCAATGGTTTAGTc > 2:478442/1‑97 (MQ=255) aagtaaCCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCAGGa > 1:2487363/1‑100 (MQ=255) aagtaaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGTACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGa < 2:2547394/100‑1 (MQ=255) gtaaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGaaa > 1:2469275/1‑100 (MQ=255) gtaaCCGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGaaa < 1:869914/100‑1 (MQ=255) ccGAGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACttt > 2:104796/1‑100 (MQ=255) | GCTGGTTGATCGGTGCCGGTAGCAGTATCTGTGGTGCTGCCGCGGTACTGGCGACTGAGCCGGTAGTGAAAGCGGAAGCCAGTAAAGTAACCGTGGCTGTTGCAACCGTTGTTATCTTCGGGACCGTCGCGATTTTCCTCTACCCGGCGATATATCCGCTGATGTCTCAATGGTTTAGTCCGGAAACTTT > NC_000913/2250117‑2250306 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |