Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,278,298	C→T	100%	intergenic (‑61/+272)	yejG ← / ← bcr	uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,278,298	0	C	T	98.9%	289.9 / ‑4.5	90	intergenic (‑61/+272)	yejG/bcr	uncharacterized protein/bicyclomycin/cysteine/sulfonamide efflux transporter
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (48/41);  total (48/42)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

CAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTT  >  NC_000913/2278202‑2278394                                                                                                 |                                                                                                 cAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaac                                                                                               <  2:1722969/100‑1 (MQ=255)   gTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATtaacaa                                                                                             >  1:1609893/1‑100 (MQ=255)       aTGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCg                                                                                         <  1:162032/100‑1 (MQ=255)        tGGACGATTGAGAGTTGTAACGAAGTCACTTATTTTCCCCGGAAGAACATTCACATCACAATTTGTTTACATCAATTTTAACCCATAATTAACACTCAGt                                                                                        >  2:2082024/1‑100 (MQ=255)         ggACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTc                                                                                       >  1:670083/1‑100 (MQ=255)          gACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCt                                                                                      >  1:2018720/1‑100 (MQ=255)          gACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCt                                                                                      <  1:642747/100‑1 (MQ=255)           aCGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCtt                                                                                     >  2:583188/1‑100 (MQ=255)           aCGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCtt                                                                                     <  1:1939002/100‑1 (MQ=255)             gATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTta                                                                                   <  1:1505864/100‑1 (MQ=255)             gATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTta                                                                                   <  1:1865390/100‑1 (MQ=255)              aTTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAAGAATCCGTCTTtat                                                                                  <  1:1742027/100‑1 (MQ=255)              aTTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTtat                                                                                  >  1:168700/1‑100 (MQ=255)                tGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATAc                                                                                >  1:687843/1‑100 (MQ=255)                 gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                               >  2:34207/1‑100 (MQ=255)                 gagaGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACa                                                                               >  1:213683/1‑100 (MQ=255)                  agagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAg                                                                              >  2:1045015/1‑100 (MQ=255)                   gagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGa                                                                             >  1:869240/1‑100 (MQ=255)                   gagTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGa                                                                             >  2:950051/1‑100 (MQ=255)                     gTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATc                                                                           >  1:1152920/1‑100 (MQ=255)                     gTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATc                                                                           >  1:2350575/1‑100 (MQ=255)                     gTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATc                                                                           >  1:758575/1‑100 (MQ=255)                      ttGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCg                                                                          >  2:2087806/1‑100 (MQ=255)                      ttGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCg                                                                          <  1:1298673/100‑1 (MQ=255)                       tGTAATGAAGTCACTTATTTTCCCCGGAAGCCCATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGt                                                                         >  2:522909/1‑100 (MQ=255)                           aTGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAg                                                                     <  1:1045023/100‑1 (MQ=255)                             gAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAt                                                                   <  1:833473/100‑1 (MQ=255)                             gAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGAt                                                                   <  2:2130198/100‑1 (MQ=255)                                gTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGa                                                                <  2:187047/100‑1 (MQ=255)                                  cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCCATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGAt                                                               >  2:128291/1‑99 (MQ=255)                                  cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              <  2:911622/100‑1 (MQ=255)                                  cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              >  1:510161/1‑100 (MQ=255)                                  cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              >  1:901547/1‑100 (MQ=255)                                  cACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGATTTCATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATg                                                              <  2:1797036/100‑1 (MQ=255)                                    cTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGcc                                                            >  1:382978/1‑100 (MQ=255)                                    cTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGcc                                                            >  1:2125964/1‑100 (MQ=255)                                     ttATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCt                                                           >  1:1509886/1‑100 (MQ=255)                                      tATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTc                                                          >  2:1098516/1‑100 (MQ=255)                                        ttttCCCCTGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGc                                                        <  2:2031931/100‑1 (MQ=255)                                        ttttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGc                                                        <  2:2193482/100‑1 (MQ=255)                                          ttCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCt                                                      >  1:2414107/1‑100 (MQ=255)                                             cccGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGc                                                    >  2:589389/1‑99 (MQ=255)                                              ccGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGt                                                  >  1:1952401/1‑100 (MQ=255)                                               cGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTg                                                 <  2:329723/100‑1 (MQ=255)                                                ggAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTgg                                                >  2:376245/1‑100 (MQ=255)                                                 gAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGc                                               <  2:1234499/100‑1 (MQ=255)                                                 gAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGc                                               <  1:1069586/100‑1 (MQ=255)                                                    gCACATTCCCTTCACAATGTGTTTACCTCAATTTTAACACATCATTAACAATCCGTCTTTACACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGcc                                              <  2:1530761/98‑1 (MQ=255)                                                    gCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCctc                                            <  2:1601466/100‑1 (MQ=255)                                                        aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                        <  1:79194/100‑1 (MQ=255)                                                        aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                        >  1:1191993/1‑100 (MQ=255)                                                        aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                        >  2:794001/1‑100 (MQ=255)                                                        aTTCACTTCACAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATg                                        <  1:583195/100‑1 (MQ=255)                                                          tcacttcacAATTTGTTTACATCAATTTGAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGAGGCCTCTATGGc                                      <  2:1077850/100‑1 (MQ=255)                                                            acttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                    >  1:2542765/1‑100 (MQ=255)                                                            acttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                    <  1:2437329/100‑1 (MQ=255)                                                            acttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                    <  2:1294823/100‑1 (MQ=255)                                                            acttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctc                                    <  1:1550693/100‑1 (MQ=255)                                                             cttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctct                                   >  2:1678000/1‑100 (MQ=255)                                                             cttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGctct                                   >  1:1882812/1‑100 (MQ=255)                                                              ttcacAATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGACCGTAAAGATTCATGCCTCGCCTGCGTGGCCTCTATGGctcag                                  <  1:1732057/100‑3 (MQ=255)                                                                cacaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAt                                >  2:2330486/1‑100 (MQ=255)                                                                cacaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAt                                <  1:2210298/100‑1 (MQ=255)                                                                cacaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAt                                <  1:380197/100‑1 (MQ=255)                                                                 acaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAtt                               <  1:1191848/100‑1 (MQ=255)                                                                 acaATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAtt                               <  1:2161353/100‑1 (MQ=255)                                                                  caATTTGTTTACATCGAGTTTAATACATGGTTAACGATCTGCCTGTATACAGCTCGTATAGATTGATGCCTCGCCTGCGTGGTCCCCATGGCTCTGa                                 <  2:630022/97‑1 (MQ=255)                                                                  caATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAttt                              >  2:2183939/1‑100 (MQ=255)                                                                  caATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAttt                              >  1:769235/1‑100 (MQ=255)                                                                  caATTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGAttt                              >  2:1164597/1‑100 (MQ=255)                                                                   aaTTTGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTa                             <  1:2000166/100‑1 (MQ=255)                                                                     tttGTTTACATCAATTTTAACACATCATTAACAATCCGTCTGTATACAGTTCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGTTCTGATTTAAg                           <  2:1966535/100‑1 (MQ=255)                                                                      ttGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGt                          <  1:1678012/100‑1 (MQ=255)                                                                       tGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTa                         >  1:697125/1‑100 (MQ=255)                                                                       tGTTTACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTa                         <  1:509822/100‑1 (MQ=255)                                                                         tttACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAat                       >  1:415582/1‑100 (MQ=255)                                                                         tttACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAat                       <  1:442934/100‑1 (MQ=255)                                                                         tttACATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAat                       <  2:2542749/100‑1 (MQ=255)                                                                          ttACCTTAAGTTGAACGCATCATTACCAATCCGTCTGTATAGCGATCGTAAAGATTGATGAGTCGCTTGCGTGGCCTCTATGGCTCTGATTTAAg                           <  2:2259230/95‑1 (MQ=255)                                                                             cATCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCt                   >  1:1430546/1‑100 (MQ=255)                                                                              aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTg                  >  2:1285296/1‑100 (MQ=255)                                                                              aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTg                  >  2:1285419/1‑100 (MQ=255)                                                                              aTCAATTTTAACACATCATTAACAATCCGTCTTTATACAAATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGCTTTAAGTAATATCTg                  >  2:2226295/1‑100 (MQ=255)                                                                                  aTTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCggg              >  1:625528/1‑100 (MQ=255)                                                                                  aTTTTAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCggg              <  1:1581817/100‑1 (MQ=255)                                                                                   ttttAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGc             >  2:1916058/1‑100 (MQ=255)                                                                                     ttAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGt           <  1:822501/100‑1 (MQ=255)                                                                                      tAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGc             >  2:1459328/1‑97 (MQ=255)                                                                                      tAACACATCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTg          >  2:893621/1‑100 (MQ=255)                                                                                         cacaTCATTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTc       >  1:1399221/1‑100 (MQ=255)                                                                                          acaTCATTTACATTCCGTCTTTATACAGATCTTAAATATTTATGCCTTGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTgg         <  2:2381617/97‑1 (MQ=255)                                                                                           catcatTAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTGAAGTAATATCTGCGAGCGTGGTCAc     >  2:2286498/1‑100 (MQ=255)                                                                                            atcattAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACg    <  1:1645762/100‑1 (MQ=255)                                                                                             tcattaACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGt   <  1:680741/100‑1 (MQ=255)                                                                                              cattaACAATCCGTCTTTATACAGATCGTAACGATTGATACCACGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGtt  >  2:837404/1‑100 (MQ=255)                                                                                              cattaACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGtt  >  1:647264/1‑100 (MQ=255)                                                                                                 |                                                                                                 CAGTCGATGGACGATTGAGAGTTGTAATGAAGTCACTTATTTTCCCCGGAAGCACATTCACTTCACAATTTGTTTACATCAATTTTAACACATCATCAACAATCCGTCTTTATACAGATCGTAAAGATTGATGCCTCGCCTGCGTGGCCTCTATGGCTCTGATTTAAGTAATATCTGCGGGCGTGGTCACGTT  >  NC_000913/2278202‑2278394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑