Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,323,291	T→A	20.7%	intergenic (+40/‑156)	atoC → / → atoD	fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,323,291	0	T	A	20.7%	209.1 / 44.0	91	intergenic (+40/‑156)	atoC/atoD	fused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (33/39);  new base A (9/10);  total (42/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCA  >  NC_000913/2323192‑2323390                                                                                                    |                                                                                                    aGTCGCCGTGCATTGACGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGAATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAAt                                                                                                     >  2:537150/1‑100 (MQ=255)  gTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAAtt                                                                                                    <  2:2380240/100‑1 (MQ=255)  gTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAAtt                                                                                                    <  2:2378735/100‑1 (MQ=255)     gCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTc                                                                                                 <  2:857864/100‑1 (MQ=255)     gCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTc                                                                                                 <  1:84609/100‑1 (MQ=255)       cGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTc                                                                                                 >  2:703306/1‑98 (MQ=255)       cGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTg                                                                                               <  1:431079/100‑1 (MQ=255)       cGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTg                                                                                               <  1:1714492/100‑1 (MQ=255)       cGTGCAGTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTg                                                                                               <  2:2080169/100‑1 (MQ=37)        gTGCATTGATGTATAAACTGCAGGAATGCGGTATCGATCCGGCGGATGTATAACACCAAATCTTGCTATGCAGAAAATTGCGCACCATGCAAATTTCTGc                                                                                              <  2:1913009/100‑1 (MQ=25)        gTGCATTGATGTATAAACTCCAGGAATACGGTATCGACCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGc                                                                                              <  1:944325/100‑1 (MQ=25)         tgtaTTGATGTATAAACTCCAGGAATACGTTATCGATCCGGCCGATCTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCa                                                                                             <  2:2042589/97‑1 (MQ=21)          gCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCAt                                                                                            >  1:1677712/1‑100 (MQ=255)          gCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCAt                                                                                            <  1:1383972/100‑1 (MQ=25)            aTTGATGTATAGACTCCAGGAATACTGTAGTGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTg                                                                                               <  2:2361232/95‑1 (MQ=255)            aTTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAg                                                                                          >  1:1213710/1‑100 (MQ=255)            aTTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAg                                                                                          <  2:1411995/100‑1 (MQ=25)             ttGATGTATAAACTCCCGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGc                                                                                         <  2:58045/100‑1 (MQ=25)             ttGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGc                                                                                         >  1:2246474/1‑100 (MQ=255)             ttGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGc                                                                                         <  2:1985954/100‑1 (MQ=25)              tGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGca                                                                                        <  1:1432926/100‑2 (MQ=25)              tGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGca                                                                                        <  1:2275302/100‑2 (MQ=25)                 tGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCt                                                                                     <  1:103457/100‑1 (MQ=255)                  gTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTc                                                                                    >  1:1448569/1‑100 (MQ=255)                   tataAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCa                                                                                   >  2:1666920/1‑100 (MQ=255)                   tataAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCa                                                                                   >  2:1245885/1‑100 (MQ=255)                     taAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCAtt                                                                                 >  1:1033562/1‑100 (MQ=255)                       aaCTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATtct                                                                               <  1:2443312/100‑1 (MQ=255)                         cTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTcc                                                                             >  2:2207790/1‑100 (MQ=255)                         cTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTcc                                                                             >  2:2208563/1‑100 (MQ=255)                         cTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTcc                                                                             >  2:2370399/1‑100 (MQ=255)                         cTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTcc                                                                             <  1:2355449/100‑1 (MQ=255)                              ggAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtata                                                                        >  1:1041753/1‑100 (MQ=255)                              ggAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtata                                                                        <  2:1677700/100‑1 (MQ=255)                              ggAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtata                                                                        >  2:1324473/1‑100 (MQ=255)                               gAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTtata                                                                        <  2:1582022/99‑1 (MQ=255)                                 aTACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAAt                                                                     <  1:2039588/100‑1 (MQ=255)                                    cGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATccc                                                                  <  1:1952509/100‑1 (MQ=255)                                       tATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCAt                                                               >  2:724947/1‑100 (MQ=255)                                       tATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCAt                                                               >  2:1952351/1‑100 (MQ=255)                                           gATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCaa                                                           >  1:1305466/1‑100 (MQ=255)                                            aTCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAAt                                                          <  1:1538714/100‑1 (MQ=255)                                               cggcggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTa                                                       <  2:1793178/100‑1 (MQ=255)                                                 gcggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATc                                                     <  1:2276560/100‑1 (MQ=255)                                                   ggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATccc                                                   >  2:1157077/1‑100 (MQ=255)                                                        tataACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCat                                              >  1:1868044/1‑100 (MQ=255)                                                        tataACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCat                                              >  2:1880719/1‑100 (MQ=255)                                                         ataACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCata                                             >  1:1748378/1‑100 (MQ=255)                                                         ataACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCata                                             >  1:961892/1‑100 (MQ=255)                                                         ataACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCata                                             <  2:290270/100‑1 (MQ=255)                                                           aaCACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATAtt                                           <  1:740043/100‑1 (MQ=255)                                                             cacCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCa                                         <  1:1666932/100‑1 (MQ=255)                                                             cacCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCa                                         <  2:2558191/100‑1 (MQ=255)                                                             cacCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCa                                         <  1:112927/100‑1 (MQ=255)                                                              acCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCaa                                        >  1:2267541/1‑100 (MQ=255)                                                                 aaaaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAAtt                                      >  1:2159452/1‑99 (MQ=255)                                                                  aaaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAg                                    >  2:2134820/1‑100 (MQ=255)                                                                   aaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGt                                   <  1:1596437/100‑1 (MQ=255)                                                                   aaCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGt                                   <  1:1447460/100‑1 (MQ=255)                                                                    agcggCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCATATAAATCCGCATCCAATTTATCCCTTCATATTCAATTAGtt                                  >  2:1796200/5‑100 (MQ=18)                                                                    aCTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGtt                                  <  1:789108/100‑1 (MQ=255)                                                                     cTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGttn                                 <  1:2346379/100‑2 (MQ=255)                                                                     cTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGtt                                  <  2:616831/99‑1 (MQ=255)                                                                      cggCTATGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTaa                                >  1:1667362/3‑100 (MQ=25)                                                                       tGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTaaa                               <  1:2315929/100‑1 (MQ=255)                                                                         cTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATa                             <  2:209660/100‑1 (MQ=255)                                                                           aTGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAAc                           >  1:535550/1‑100 (MQ=25)                                                                            tGCAGACAATTGCGCCCTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAACTCCCCATCCAATTTGTACCTTCATATTCAATTAGTTACATAACt                          >  2:1575830/1‑100 (MQ=21)                                                                            tGCAGAAATTTGCACAGTGCGCAATTTTCTGCATATCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACt                          <  2:1550084/100‑1 (MQ=255)                                                                            tGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACt                          >  1:1225476/1‑100 (MQ=255)                                                                            tGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACt                          <  1:1469462/100‑1 (MQ=255)                                                                            tGCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACt                          >  2:737747/1‑100 (MQ=37)                                                                             gCAGAACTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTa                         <  2:2217895/100‑1 (MQ=255)                                                                             gCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTa                         >  2:2447357/1‑100 (MQ=255)                                                                             gCAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTa                         >  1:2355712/1‑100 (MQ=25)                                                                             gCAGAAAATTGCGCACTGTGCAAATTTCTGCACAGACGCTCGCTCTCCTTATAAATACCAATCAAATTTATCCCATCATATTCAATTAGTTAAATAACTa                         >  2:1401273/1‑100 (MQ=16)                                                                              cAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTaa                        >  1:2008839/1‑100 (MQ=255)                                                                              cAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTaa                        >  2:239752/1‑100 (MQ=37)                                                                              cAGAAAATTGCGCACTGTGCAAATTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTaa                        >  1:466249/1‑100 (MQ=37)                                                                               aGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTaaa                       <  1:1806524/100‑1 (MQ=255)                                                                                  aaTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATcc                    >  2:156908/1‑100 (MQ=255)                                                                                  aaTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATcc                    <  2:1225468/100‑1 (MQ=255)                                                                                  aaTTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATcc                    <  1:880199/100‑1 (MQ=255)                                                                                     ttGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCaat                 <  1:2158550/100‑1 (MQ=255)                                                                                     ttGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCaat                 <  2:1711158/100‑1 (MQ=255)                                                                                     ttGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCaat                 <  1:434575/100‑1 (MQ=255)                                                                                        cacaGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCaataat              <  2:1969266/100‑1 (MQ=255)                                                                                         acaGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAAtc             >  1:2119784/1‑100 (MQ=255)                                                                                            gTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCa          <  1:1140838/100‑1 (MQ=255)                                                                                               cgcAATTTTCTGCATAGCCGCTGATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTAAtt        >  2:1484473/1‑99 (MQ=255)                                                                                                gcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCt      >  2:1752598/1‑100 (MQ=255)                                                                                                gcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCt      >  2:373487/1‑100 (MQ=255)                                                                                                 cAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTg     <  1:1639361/100‑1 (MQ=255)                                                                                                   aTTTTCTGCATAGCCGCTCATTCTCCTTATCAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGc   >  2:2338373/1‑100 (MQ=255)                                                                                                    ttttCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCa  <  1:928179/100‑1 (MQ=255)                                                                                                    |                                                                                                    AGTCGCCGTGCATTGATGTATAAACTCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCA  >  NC_000913/2323192‑2323390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑