Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,449,615 C→G 19.6% G145G (GGG→GGC) ‡ yfcO ← DUF2544 family putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,449,6150CG19.6% 252.6 / 61.5 107G145G (GGG→GGCyfcODUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (47/39);  new base G (9/12);  total (56/51)
Fisher's exact test for biased strand distribution p-value = 3.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.76e-02

Only 100 of 112 total aligned reads displayed.

CAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACC  >  NC_000913/2449516‑2449714
                                                                                                    |                                                                                                   
cAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGc                                                                                                     <  1:1697510/100‑1 (MQ=255)
 aGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGcc                                                                                                    <  1:67606/100‑1 (MQ=255)
       aGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGACCCCTGAt                                                                                              >  2:366911/1‑100 (MQ=255)
          aGTTTTTACTTACTGACATAATTCGCATTCCATGAGACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACg                                                                                           <  1:1472487/100‑1 (MQ=255)
          aGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATag                                                                                            <  2:1239365/100‑2 (MQ=255)
          aGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACg                                                                                           <  2:1462340/100‑1 (MQ=255)
           gTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTTATACGt                                                                                          >  2:2087525/1‑100 (MQ=255)
            ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATagtc                                                                                          <  1:2198840/100‑4 (MQ=38)
            ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTc                                                                                         <  2:579604/100‑1 (MQ=255)
            ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTc                                                                                         <  1:2548551/100‑1 (MQ=255)
             tttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATagtca                                                                                         <  1:900328/100‑5 (MQ=38)
               tGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACg                                                                                      <  1:849901/100‑1 (MQ=255)
                 aCTTACTGACATAATTCTCATTCCATGAAACGTCATACGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGcc                                                                                    >  1:270163/1‑100 (MQ=255)
                  cTTACTGACATAATTGTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgaca                                                                                   <  1:1582688/100‑4 (MQ=25)
                   ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacac                                                                                  <  1:1511023/100‑5 (MQ=25)
                   ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacac                                                                                  <  1:1184045/100‑5 (MQ=25)
                   ttACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacac                                                                                  <  2:444922/100‑5 (MQ=37)
                    tACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGccccc                                                                                 <  2:2522114/100‑1 (MQ=255)
                     aCTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCt                                                                                >  1:2229503/1‑100 (MQ=255)
                      cTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTg                                                                               <  1:935663/100‑1 (MQ=255)
                        gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTCACTATCAGGCCCCTGATACGTCACGCCCCCTGga                                                                             <  2:2552084/100‑1 (MQ=255)
                         aCATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACGTATCAGGGGCCTGATAGTCACgacacgatttg                                                                            <  2:1940078/100‑11 (MQ=21)
                          cATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGgaga                                                                           >  1:2114237/1‑100 (MQ=255)
                           aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGagag                                                                          >  1:771113/1‑100 (MQ=255)
                           aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGagag                                                                          <  1:988917/100‑1 (MQ=255)
                            tAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAgg                                                                         <  1:2338705/100‑1 (MQ=255)
                               ttCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCtt                                                                      >  1:1132391/1‑100 (MQ=255)
                               ttCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCtt                                                                      >  1:1165312/1‑100 (MQ=255)
                                 ctcATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCa                                                                    <  2:1701709/100‑1 (MQ=255)
                                  tcATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCag                                                                   <  1:2310292/100‑1 (MQ=255)
                                    aTTCCATGAAACGTCATAAGGTTGAACAGAAACTTATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                 <  2:627969/100‑1 (MQ=255)
                                    aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                 <  1:895163/100‑1 (MQ=255)
                                    aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCagag                                                                 >  2:225790/1‑100 (MQ=255)
                                    aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCag                                                                   >  1:640378/1‑98 (MQ=255)
                                     ttCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGt                                                                >  1:1314239/1‑100 (MQ=255)
                                     ttCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATATGGCGCCTGATACGTCACGCCCCCTGAAGAGCCATCaga                                                                  >  2:801806/1‑98 (MQ=255)
                                      tCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGCTACGTCACGCCCCCTGGAGAGGCTTCAGAGt                                                                >  2:703993/1‑99 (MQ=255)
                                       ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtca                                                              >  1:1538678/1‑100 (MQ=255)
                                       ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtca                                                              >  1:2250036/1‑100 (MQ=255)
                                       ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtca                                                              >  1:13988/1‑100 (MQ=255)
                                        cATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcat                                                             >  1:1738622/1‑100 (MQ=255)
                                        cATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcat                                                             >  2:1687271/1‑100 (MQ=255)
                                         aTGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcatt                                                            <  1:249851/100‑1 (MQ=255)
                                          tGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcattc                                                           <  2:610079/100‑1 (MQ=255)
                                            aaaCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGtcattcat                                                         <  1:1228467/100‑1 (MQ=255)
                                              aCGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataa                                                       <  1:225792/100‑1 (MQ=255)
                                                gTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCataata                                                     <  2:502123/100‑1 (MQ=255)
                                                 tCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGAGTATAAGGCCCCTGATCAGTAACGCCACCTGGAGAGGCTTCACAGTCATCGAtaataa                                                    >  2:758236/1‑100 (MQ=255)
                                                   aTAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaacc                                                  <  2:446188/100‑1 (MQ=255)
                                                   aTAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaacc                                                  <  2:447405/100‑1 (MQ=255)
                                                       ggTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaaccaacc                                              >  1:208585/1‑100 (MQ=255)
                                                       ggTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaaccaacc                                              >  1:978355/1‑100 (MQ=255)
                                                         ttGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCAt                                            <  2:2090136/100‑1 (MQ=255)
                                                             aCAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGAc                                        >  2:1303574/1‑100 (MQ=255)
                                                                gAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAg                                     >  2:1066272/1‑100 (MQ=255)
                                                                gAAACGGATGGACAAATCGGGTCGTTACTATCAGGCCCCTGATACGGCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAg                                     >  2:2040063/1‑100 (MQ=255)
                                                                      gATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGt                               <  2:2001881/100‑1 (MQ=255)
                                                                       aTGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTa                              <  1:580623/100‑1 (MQ=255)
                                                                        tGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTaa                             >  1:2077457/1‑100 (MQ=255)
                                                                         ggACAAATCGTGTCGTGACTATCAGGCCCCTGATACGACACGCCCCCTGGAGAGGCTCCAGAGTCATTCAAAATAACCAACCATGGACAAGAAAAGTaa                             >  2:1115991/1‑99 (MQ=255)
                                                                          gACAAATCGTGTCGTGATTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGa                           >  1:1572623/1‑100 (MQ=255)
                                                                          gACAAATCGTGTCCTGACTATCAGGCCCCTGGTACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCAGGGACAAGAAAAGTAAGa                           >  1:1725512/1‑100 (MQ=255)
                                                                           aCAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAt                          >  1:982301/1‑100 (MQ=255)
                                                                           aCAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAt                          <  2:1098496/100‑1 (MQ=255)
                                                                            cAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAtt                         >  2:1898912/1‑100 (MQ=255)
                                                                             aaaTCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGTCCAAGAAAAGTAAg                            >  1:443008/1‑96 (MQ=255)
                                                                              aaTCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCa                       >  1:2036447/1‑100 (MQ=255)
                                                                              aaTCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCa                       >  1:2036819/1‑100 (MQ=255)
                                                                                tcgtgtcgtgACTATCAGGCCCCTGATGCGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGAATCCAt                     >  2:113805/1‑100 (MQ=255)
                                                                                 gggggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtc                    >  1:630516/6‑100 (MQ=25)
                                                                                 gggggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtc                    >  2:450929/6‑100 (MQ=25)
                                                                                   gggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAAtctc                  >  2:366289/4‑100 (MQ=37)
                                                                                    gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGACAAGTAAGATTCAATctct                 >  2:2209606/1‑100 (MQ=255)
                                                                                    gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGagccgaatca                  >  2:360058/1‑90 (MQ=255)
                                                                                    ggcgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATctct                 >  1:1403256/3‑100 (MQ=37)
                                                                                     tcgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTg                <  1:2020752/100‑1 (MQ=255)
                                                                                      cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc               >  1:429311/1‑100 (MQ=38)
                                                                                      cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc               >  1:1413950/1‑100 (MQ=38)
                                                                                      cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc               >  1:683753/1‑100 (MQ=38)
                                                                                      cgtgACGTATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc               >  2:2202790/1‑100 (MQ=38)
                                                                                      cgtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc               >  2:867610/1‑100 (MQ=255)
                                                                                       gtgacgtATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa              <  2:630509/94‑1 (MQ=38)
                                                                                       gtgacgtATCAGGGGCCTGATAGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa              >  1:156223/7‑100 (MQ=38)
                                                                                       gtgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa              >  2:1235169/1‑100 (MQ=255)
                                                                                        tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag             <  1:1097809/100‑1 (MQ=255)
                                                                                        tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag             <  1:486285/100‑1 (MQ=255)
                                                                                        tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag             <  1:310254/100‑1 (MQ=255)
                                                                                        tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCag             <  1:2136581/100‑1 (MQ=255)
                                                                                        tgACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCa              >  2:464052/1‑99 (MQ=255)
                                                                                         gACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCaga            >  1:1697477/1‑100 (MQ=255)
                                                                                         gACTATCAGGCCCCTGATACCTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCaga            <  2:2467262/100‑1 (MQ=255)
                                                                                          aCTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCagag           <  2:1165304/100‑1 (MQ=255)
                                                                                          aCTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCagag           <  1:606492/100‑1 (MQ=255)
                                                                                             tATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGAc         <  2:460642/100‑1 (MQ=255)
                                                                                               tCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACaa       >  1:1588234/1‑100 (MQ=255)
                                                                                                 aGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATa     <  2:443004/100‑1 (MQ=255)
                                                                                                  ggCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATaa    >  1:145700/1‑100 (MQ=255)
                                                                                                    ccccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAcc  >  1:293986/1‑100 (MQ=255)
                                                                                                    ccccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAcc  <  1:1804935/100‑1 (MQ=255)
                                                                                                    ccccTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAAcc  <  1:2412908/100‑1 (MQ=255)
                                                                                                    |                                                                                                   
CAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACC  >  NC_000913/2449516‑2449714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: