| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,495,173 | T→A | 100% | A40A (GCA→GCT) | yfdY ← | DUF2545 family putative inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,495,173 | 0 | T | A | 96.6% | 278.4 / 0.4 | 88 | A40A (GCA→GCT) | yfdY | DUF2545 family putative inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base A (48/37); total (49/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01 | |||||||||||
AGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGC > NC_000913/2495076‑2495268 | aGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGc < 2:874940/100‑1 (MQ=255) cGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAg > 1:498004/1‑100 (MQ=255) catcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCg < 2:2478272/97‑1 (MQ=255) catcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAgg < 1:1819059/100‑1 (MQ=255) catcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAgg < 2:397084/100‑1 (MQ=255) catcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAgg > 2:710611/1‑100 (MQ=255) atcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGa > 1:988513/1‑100 (MQ=255) atcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGa > 1:1451861/1‑100 (MQ=255) tcaAGCCACAAGCCGGGGCAAGAAACATAAATATCCGCGTCACTAGTTCATTACCGCCAGTTAACAACATGGTGAAGTAATAAATGAGAGCAGCGAGGa > 2:1704166/1‑99 (MQ=255) caAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATc > 1:1270468/1‑100 (MQ=255) caAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATc > 2:1246066/1‑100 (MQ=255) aGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCAc > 1:2303098/1‑100 (MQ=255) aGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCAc > 2:1811013/1‑100 (MQ=255) ccACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCa < 1:1508910/100‑1 (MQ=255) ccACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCa < 1:364360/100‑1 (MQ=255) ccACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCa > 1:1096987/1‑100 (MQ=255) cacaAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGCAGTAATAAATGAGAGCAGCGAGGATCACCAt > 1:1941473/1‑100 (MQ=255) cacaAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCAt > 1:954489/1‑100 (MQ=255) cacaAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGCAATAAATGAGAGCAGCGAGGATCACCAt > 2:2013998/1‑100 (MQ=255) aGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCg < 1:1336498/100‑1 (MQ=255) aGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCg > 1:1064884/1‑100 (MQ=255) ccGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCgaa > 2:668464/1‑100 (MQ=255) cGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGCGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCgaag > 1:919328/1‑100 (MQ=255) tgggCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGCTAACCACATGGTGAAGTAATAAACGAGAGCAGCGAGGATCACCATGCCgaag > 2:2192730/2‑99 (MQ=255) ggCAAGAAATATAAATATCCCCGTCACTAGTTTATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGaagaaa < 1:1792488/100‑1 (MQ=255) cAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGaagaaaga > 1:2144726/1‑100 (MQ=255) cAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGaagaaaga > 2:823536/1‑100 (MQ=255) cAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGaagaaaga > 1:127909/1‑100 (MQ=255) aGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAaagaag < 1:2010939/100‑1 (MQ=255) aGAAACATAAATATCCCCGTCACTAGGTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAagaaagaa > 2:887047/1‑99 (MQ=255) aaCATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAgaga > 1:697352/1‑100 (MQ=255) aaCATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAgaga > 1:618278/1‑100 (MQ=255) cATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGAcc > 2:1933700/1‑100 (MQ=255) cATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGAcc < 1:1927700/100‑1 (MQ=255) tAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGAccgc > 1:710208/1‑100 (MQ=255) aaaTATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGAccgcc < 2:33771/100‑1 (MQ=255) aaTATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGAccgccg < 1:1466931/100‑1 (MQ=255) atatCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGTTCACCATGCCGAAGAAAGAAGAGACCGCCGa < 2:2152796/100‑1 (MQ=255) atCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATa > 1:2560706/1‑100 (MQ=255) ccccGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATAcc > 2:1449328/1‑100 (MQ=255) cccGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCa < 2:437989/100‑1 (MQ=255) ccGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCAc > 1:833727/1‑100 (MQ=255) ccGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCAc < 2:2293675/100‑1 (MQ=255) cGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACg < 2:992942/100‑1 (MQ=255) cGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACg > 1:1779703/1‑100 (MQ=255) tCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGtt > 2:1562246/1‑100 (MQ=255) cACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTc < 2:2041743/100‑1 (MQ=255) aCTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCa < 1:2245068/100‑1 (MQ=255) aCTAGTTCATTACCGCCAGTTAACCACACGGTGAAGTAATAAATGAGAGCAGCGAGGATCCTCATGCCGAAAAAAGAAGAGAGCGCTGATACCa < 2:1343231/94‑1 (MQ=255) aGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTa > 1:2348700/1‑100 (MQ=255) aGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTa < 2:168040/100‑1 (MQ=255) gTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTAc > 1:2231360/1‑100 (MQ=255) gTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTAc > 1:1194394/1‑100 (MQ=255) gTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTAc < 2:1403744/100‑1 (MQ=255) ttCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTAcn < 2:618271/100‑2 (MQ=255) ttCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTAcc > 2:817269/1‑100 (MQ=255) cATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTg < 2:1194386/100‑1 (MQ=255) aCCGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCg < 1:1325960/100‑1 (MQ=255) ccGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGa > 1:2002457/1‑100 (MQ=255) ccGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGa > 1:462398/1‑100 (MQ=255) ccGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGa < 1:1045074/100‑1 (MQ=255) ccGCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGACCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGa > 2:1513092/1‑100 (MQ=255) gCCAGTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATg < 2:127907/100‑1 (MQ=255) gTTAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGc < 1:1792883/100‑1 (MQ=255) tAACCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATAACACGTTCAGTACCTGACCGATGTAGCCg > 1:2137893/1‑100 (MQ=255) aaCCACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGccac > 2:1762260/1‑98 (MQ=255) ccACATGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCtt > 1:1570171/1‑100 (MQ=255) cacaTGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTa < 1:2255678/100‑1 (MQ=255) cacaTGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTa > 1:1872737/1‑100 (MQ=255) cacaTGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTa > 1:191133/1‑100 (MQ=255) acaTGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTAc < 2:833720/100‑1 (MQ=255) acaTGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGCTGTAGCCGCtt > 2:189144/1‑98 (MQ=255) acaTGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTAc > 1:855629/1‑100 (MQ=255) acaTGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTAc < 2:1735442/100‑1 (MQ=255) tGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCa < 2:2033511/100‑1 (MQ=255) tGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCa > 1:2457888/1‑100 (MQ=255) tGGTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCa < 1:1292583/100‑1 (MQ=255) ggTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAg < 1:1074928/100‑1 (MQ=255) gTGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACAACGTTCCGTACCTGACCGATGTAGCCGCTTACGCAGa > 2:1993994/1‑100 (MQ=255) tGAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGAc > 2:1651074/1‑100 (MQ=255) gAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGAc < 1:459829/99‑1 (MQ=255) gAAGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACa > 1:950325/1‑100 (MQ=255) gAAGTAATAAAGGAGAGCAGCGAGGATAACCATGCCGAAGAAAGAAGATACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACa < 2:248369/100‑1 (MQ=255) aaGTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACaa < 2:2002236/100‑1 (MQ=255) gTAATAAATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATa < 1:2379651/100‑1 (MQ=255) aataaATGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATaca > 2:950728/1‑100 (MQ=255) aaaTGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAg < 2:1225513/100‑1 (MQ=255) tGAGAGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGc < 2:1096979/100‑1 (MQ=255) | AGCGAATCATCAAGCCACAAGCCGGGGCAAGAAACATAAATATCCCCGTCACTAGTTCATTACCGCCAGTTAACCACATGGTGAAGTAATAAATGAGTGCAGCGAGGATCACCATGCCGAAGAAAGAAGAGACCGCCGATACCACGTTCAGTACCTGACCGATGTAGCCGCTTACGCAGACAATACACAGGGC > NC_000913/2495076‑2495268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |