Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,530,925	T→C	100%	Q103Q (CAA→CAG)	zipA ←	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,530,925	0	T	C	98.9%	318.8 / ‑4.8	92	Q103Q (CAA→CAG)	zipA	FtsZ stabilizer
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (50/41);  total (51/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

TCAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATGATTC  >  NC_000913/2530830‑2531021                                                                                                |                                                                                                 tCAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGAc                                                                                              >  2:476090/1‑100 (MQ=255)    ggCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGAcgcg                                                                                           <  1:1047648/100‑1 (MQ=255)      cTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGc                                                                                         <  2:573067/100‑1 (MQ=255)      cTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGc                                                                                         <  2:2047320/100‑1 (MQ=255)         cGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTgc                                                                                      <  1:2281485/100‑1 (MQ=255)          ggCTGATGGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTg                                                                                       <  2:597380/98‑1 (MQ=255)          ggCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTgcg                                                                                     <  2:2469105/100‑1 (MQ=255)          ggCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTgcg                                                                                     >  1:2259698/1‑100 (MQ=255)          ggCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTgcg                                                                                     <  1:1892335/100‑1 (MQ=255)           gCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTgcgc                                                                                    <  2:1606103/100‑1 (MQ=255)           gCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTgcgc                                                                                    >  2:1433777/1‑100 (MQ=255)            cTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCa                                                                                   >  2:1252712/1‑100 (MQ=255)               aTAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGAc                                                                                >  2:506023/1‑100 (MQ=255)                tAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACg                                                                               >  2:1156786/1‑100 (MQ=255)                tAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACg                                                                               <  1:1411699/100‑1 (MQ=255)                  ggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCa                                                                             >  2:9469/1‑100 (MQ=255)                  ggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCa                                                                             >  2:831759/1‑100 (MQ=255)                  ggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCa                                                                             >  1:2104420/1‑100 (MQ=255)                  ggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCa                                                                             >  1:2104649/1‑100 (MQ=255)                  ggcaggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCa                                                                             >  1:274499/1‑100 (MQ=255)                      ggcTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAg                                                                         >  1:377288/1‑100 (MQ=255)                          gctgcACCGGCTGCGCTGGATGAGGAGCATGATGCGGCGGTACCTGCGCCTCCGGCGACTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATCAGGCgg                                                                     >  2:1795061/1‑100 (MQ=255)                           ctgcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCgg                                                                     >  2:2534715/1‑99 (MQ=255)                           ctgcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGt                                                                    <  2:2491266/100‑1 (MQ=255)                             gcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTg                                                                  <  1:151274/100‑1 (MQ=255)                             gcACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTCCGCAGACGCATCAGGCgg                                                                     >  2:2218484/1‑97 (MQ=255)                             gcACCGGCTGCGCTGGATGCGGAGCATGCTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGCCGCGGCTGCGCAGACGCATAAGGCGGTTg                                                                  >  2:2116512/1‑100 (MQ=255)                               aCCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGt                                                                <  1:2199565/100‑1 (MQ=255)                               aCCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGt                                                                <  2:1217700/100‑1 (MQ=255)                                ccGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGGGGTTGGTa                                                               >  1:922709/1‑100 (MQ=255)                                 cGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTAc                                                              >  2:651123/1‑100 (MQ=255)                                 cGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTAc                                                              <  2:1089236/100‑1 (MQ=255)                                    cTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTgg                                                           <  1:1257347/100‑1 (MQ=255)                                    cTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTgg                                                           <  1:552208/100‑1 (MQ=255)                                     tGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGgt                                                          <  2:37914/100‑1 (MQ=255)                                      gcgcTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGgtg                                                         >  1:1456705/1‑100 (MQ=255)                                      gcgcTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGgtg                                                         <  1:1005110/100‑1 (MQ=255)                                        gcTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGtt                                                       >  1:1085385/1‑100 (MQ=255)                                        gcTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGtt                                                       >  2:1782002/1‑100 (MQ=255)                                         cTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTg                                                      <  2:1628625/100‑1 (MQ=255)                                         cTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTg                                                      >  1:375831/1‑100 (MQ=255)                                         cTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTg                                                      >  1:1410773/1‑100 (MQ=255)                                             aTGCGGAGCATTGTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcggc                                                  <  2:1791827/100‑1 (MQ=255)                                             aTGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcggc                                                  >  1:27831/1‑100 (MQ=255)                                             aTGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcggc                                                  >  2:1062753/1‑100 (MQ=255)                                              tGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGGCGCGGCTGCGCAGACGCATAAGCCGGTAGGTACCGGTGTTgcggcg                                                 >  1:2556741/1‑100 (MQ=255)                                              tGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcggcg                                                 >  2:2411066/1‑100 (MQ=255)                                              tGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGAGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTgcggcg                                                 >  2:1641285/1‑100 (MQ=255)                                                cGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgac                                               <  1:430148/100‑1 (MQ=255)                                                cGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgac                                               >  1:308893/1‑100 (MQ=255)                                                  gAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacgg                                             <  2:1964761/100‑1 (MQ=255)                                                   aGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacgga                                            <  1:1644070/100‑1 (MQ=255)                                                    gCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacggac                                           <  2:483516/100‑1 (MQ=255)                                                      aTGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCgacggacg                                          >  2:564234/1‑99 (MQ=255)                                                       tGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGag                                        <  2:1911757/100‑1 (MQ=255)                                                        gTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGagc                                       <  1:1127289/100‑1 (MQ=255)                                                          tGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGagcag                                     <  1:1864769/100‑1 (MQ=255)                                                          tGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGagcag                                     >  2:1284660/1‑100 (MQ=255)                                                            cggcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGcc                                   >  2:2542092/1‑100 (MQ=255)                                                             ggcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCt                                  <  1:92087/100‑1 (MQ=255)                                                              gcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATACGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTc                                 >  2:1479588/1‑100 (MQ=255)                                                              gcggTACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTc                                 >  1:639290/1‑100 (MQ=255)                                                                  tACCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGc                             >  1:985161/1‑100 (MQ=255)                                                                   aCCTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCt                            >  2:1691057/1‑100 (MQ=255)                                                                    ccggcgcTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTGGGGACTGGTGTTGCGGGGACGGACGAGCAGCCTCATGCTc                           >  2:700441/4‑100 (MQ=255)                                                                    ccTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCt                            <  1:1660812/99‑1 (MQ=255)                                                                    ccTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTc                           <  2:239027/100‑1 (MQ=255)                                                                    ccTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTc                           >  1:765121/1‑100 (MQ=255)                                                                     cTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTcc                          >  2:908177/1‑100 (MQ=255)                                                                     cTGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTcc                          <  1:992287/100‑1 (MQ=255)                                                                      tGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCt                         >  1:921036/1‑100 (MQ=255)                                                                      tGCGCTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCt                         >  2:76035/1‑100 (MQ=255)                                                                        cgcTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGa                       >  1:2085373/1‑100 (MQ=255)                                                                         gcTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAg                      >  2:72548/1‑100 (MQ=255)                                                                         gcTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCAGAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAg                      <  2:532435/100‑1 (MQ=255)                                                                          cTTCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGc                     <  2:158619/100‑1 (MQ=255)                                                                            tCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGt                   <  2:1538777/100‑1 (MQ=255)                                                                            tCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGt                   <  1:600390/100‑1 (MQ=255)                                                                            tCAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGt                   >  2:1790941/1‑100 (MQ=255)                                                                             cAGGCGGCTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGtt                  >  1:341554/1‑100 (MQ=255)                                                                               ggcggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATCAGGCGGGTGGTACTGGTGTTGCGGCGAAGGAGGAGCAGACTCATGCTCCTGAGCGTTAg                >  2:2497813/1‑100 (MQ=255)                                                                               ggcggcTGCTGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAg                >  2:87454/1‑100 (MQ=255)                                                                                 cggcTGCTGTACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACCGGTGTTGCGGCGACGTACGAGCAGCCTCATGCTCCTGAGCGTTAGcc              <  2:2437893/100‑1 (MQ=255)                                                                                   gctgctGGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCgg            >  1:165762/1‑100 (MQ=255)                                                                                    ctgctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCggg           <  1:66684/100‑1 (MQ=255)                                                                                    ctgctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCggg           <  1:983300/100‑1 (MQ=255)                                                                                    ctgctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCggg           <  2:1783990/100‑1 (MQ=255)                                                                                      gctgGACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGc         <  1:601870/100‑1 (MQ=255)                                                                                         ggACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATg      <  1:651130/100‑1 (MQ=255)                                                                                         ggACCGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCCCCTGAGCGTTAGCCGGGGCATg      >  2:1793906/1‑100 (MQ=255)                                                                                            ccGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATGAtt   >  2:1708101/1‑100 (MQ=255)                                                                                             cGGCTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATGATTc  >  1:2106225/1‑100 (MQ=255)                                                                                                |                                                                                                 TCAGGCTGCGGCTGATAGGCAGGCTGCTGCACCGGCTGCGCTGGATGCGGAGCATGTTGCGGCGGTACCTGCGCTTCAGGCGGCTGCTGGACCGGTTGACGCGGCTGCGCAGACGCATAAGGCGGTTGGTACTGGTGTTGCGGCGACGGACGAGCAGCCTCATGCTCCTGAGCGTTAGCCGGGGCATGATTC  >  NC_000913/2530830‑2531021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑