Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,534,206 T→G 100% R47R (CGT→CGG)  ptsI → PEP‑protein phosphotransferase of PTS system (enzyme I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,534,2060TG96.7% 348.8 / ‑1.4 102R47R (CGT→CGG) ptsIPEP‑protein phosphotransferase of PTS system (enzyme I)
Reads supporting (aligned to +/- strand):  ref base T (2/2);  new base G (43/55);  total (45/57)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

AAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTG  >  NC_000913/2534109‑2534302
                                                                                                 |                                                                                                
aaGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGc                                                                                                <  1:1470863/100‑1 (MQ=255)
 aGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGc                                                                                                >  2:404475/1‑99 (MQ=255)
    tctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAg                                                                                            <  2:2466201/100‑1 (MQ=255)
    tctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGG‑AAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAg                                                                                            <  2:404054/99‑1 (MQ=255)
     ctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAgg                                                                                           >  1:1792312/1‑100 (MQ=255)
     ctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAgg                                                                                           <  2:2059383/100‑1 (MQ=255)
     ctGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAgg                                                                                           <  1:2467903/100‑1 (MQ=255)
      tGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGc                                                                                          <  1:1991445/100‑1 (MQ=255)
       gCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCa                                                                                         >  1:1077601/1‑100 (MQ=255)
        cTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCAt                                                                                        >  1:504861/1‑100 (MQ=255)
         ttCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATc                                                                                       <  1:925044/100‑1 (MQ=255)
           cTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACTTTTTCTTAGCGGTCGTGCCAAGGCATCAg                                                                                     <  2:1260561/100‑1 (MQ=255)
           cTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAg                                                                                     <  1:354947/100‑1 (MQ=255)
             gAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGcc                                                                                   <  1:962711/100‑1 (MQ=255)
             gAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGcc                                                                                   >  2:1327304/1‑100 (MQ=255)
              aaaGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGccc                                                                                  <  1:1550639/100‑1 (MQ=255)
              aaaGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGccc                                                                                  <  2:1973830/100‑1 (MQ=255)
               aagaagACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCa                                                                                 >  1:1560180/1‑100 (MQ=255)
                   ngaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGAGGTCGTGCTAAGGCATCAGCCCAGCTg                                                                             >  2:1502134/2‑100 (MQ=255)
                   agaCGAAATTGTCATTGACCGGAACAAAATTTCTGCCGACCAGGTTGATAAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCAAAGCTg                                                                             >  2:939026/1‑100 (MQ=255)
                   agaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTg                                                                             <  1:2143800/100‑1 (MQ=255)
                   agaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTg                                                                             <  2:2181385/100‑1 (MQ=255)
                   agaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTg                                                                             <  1:665184/100‑1 (MQ=255)
                    gaCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTgg                                                                            >  1:2442109/1‑100 (MQ=255)
                     aCGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGa                                                                           <  2:1629194/100‑1 (MQ=255)
                        aaaTTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAAc                                                                        >  2:1676050/1‑100 (MQ=255)
                        aaaTTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAAc                                                                        <  1:1270064/100‑1 (MQ=255)
                         aatttgcATTGAACGGAAAAAAATTTCTGTCGACCAGGGTGATCAGCAAGTTGAACGTTTTCTGAGCGGTCAGGACATGGCATATGCCCAGCTGGAAACg                                                                       <  2:111200/94‑1 (MQ=255)
                           ttGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGAt                                                                     <  2:311300/100‑1 (MQ=255)
                           ttGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGAt                                                                     <  1:1529946/100‑1 (MQ=255)
                            tGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATc                                                                    <  1:2460338/100‑1 (MQ=255)
                            tGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCAGCAGCCCAGCTGGAAACGATc                                                                    <  2:1932845/100‑1 (MQ=255)
                              tCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCaa                                                                  >  2:1719155/1‑100 (MQ=255)
                               cATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCaaa                                                                 >  2:222829/1‑100 (MQ=255)
                               cATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCaaa                                                                 <  1:1050544/100‑1 (MQ=255)
                               cATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCaaa                                                                 <  2:603770/100‑1 (MQ=255)
                                 ttGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAAc                                                               <  2:897232/100‑1 (MQ=255)
                                    aCCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGaa                                                            <  1:3149/100‑1 (MQ=255)
                                     ccGGAAAAAAATTTCTGCCTACCATGTTGATCTGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGTTGGAAACGATCaa                                                                  <  2:2410588/93‑1 (MQ=255)
                                     ccGGAAAAAAATTTCTGCCGACCAGGTTGATCATGAAGTTGAACGTTTTCTGAGCGGTCGGCCCAAGGCATCCGCCCAGCTGGAAACGATCAAAACGaaa                                                           >  2:618303/1‑100 (MQ=255)
                                     ccGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGaaa                                                           >  2:1832444/1‑100 (MQ=255)
                                      cGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAg                                                          <  1:2493627/100‑1 (MQ=255)
                                      cGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAg                                                          >  1:2495004/1‑100 (MQ=255)
                                       ggAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGc                                                         <  1:388329/100‑1 (MQ=255)
                                       ggAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGc                                                         >  1:874553/1‑100 (MQ=255)
                                       ggAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGc                                                         >  1:875993/1‑100 (MQ=255)
                                          aaaaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTgg                                                      <  2:616625/100‑1 (MQ=255)
                                           aaaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGt                                                     >  2:1675390/1‑100 (MQ=255)
                                            aaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTg                                                    >  2:2265059/1‑100 (MQ=255)
                                            aaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTg                                                    >  1:700552/1‑100 (MQ=255)
                                            aaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTg                                                    <  2:2451594/100‑1 (MQ=255)
                                            aaaaTTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTg                                                    <  2:652206/100‑1 (MQ=255)
                                                tttCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAAc                                                <  1:2366179/100‑1 (MQ=255)
                                                tttCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAAc                                                >  1:81538/1‑100 (MQ=255)
                                                 ttCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACg                                               <  1:663918/100‑1 (MQ=255)
                                                 ttCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACg                                               <  1:113115/100‑1 (MQ=255)
                                                    tGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTc                                            <  1:1327313/100‑1 (MQ=255)
                                                    tGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTc                                            <  2:301414/100‑1 (MQ=255)
                                                      ccGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCgg                                          <  1:287980/100‑1 (MQ=255)
                                                       cGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGt                                         <  1:1584100/100‑1 (MQ=255)
                                                        gACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGt                                         <  1:1766044/99‑1 (MQ=255)
                                                        gACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTg                                        <  1:249447/100‑1 (MQ=255)
                                                         aCCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGtaa                                       <  1:1739537/100‑3 (MQ=255)
                                                           cAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaag                                     <  2:2193636/100‑1 (MQ=255)
                                                           cAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGGgaag                                     >  2:112888/1‑100 (MQ=255)
                                                            aGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaaga                                    >  2:928274/1‑100 (MQ=255)
                                                            aGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaaga                                    >  1:186835/1‑100 (MQ=255)
                                                            aGGTTGATCAGGAAGTTGAACGTTTTCTCAGCGGGCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaaga                                    <  1:1012764/100‑1 (MQ=255)
                                                             gggtGATCAGGAAGTTGTACGGTTTCTGAGCGGTCGGGCCAAGGCATAAGCACAGCTGGAAACGGTCAGAACGAAAGCTGGTGAAAGCTTGGGTgaagaa                                   >  2:2272451/4‑100 (MQ=255)
                                                             ggTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTgaagaa                                   >  1:717902/1‑100 (MQ=255)
                                                              gTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGaaa                                  <  1:244306/100‑1 (MQ=255)
                                                                  agcAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaaga                              <  1:1136865/98‑1 (MQ=255)
                                                                  aTCAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaaga                              <  1:307738/100‑1 (MQ=255)
                                                                    cAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaagaag                            >  1:1163614/1‑100 (MQ=255)
                                                                    cAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaagaag                            >  2:344344/1‑100 (MQ=255)
                                                                    cAGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAaagaag                            >  2:839895/1‑100 (MQ=255)
                                                                     aGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGc                           >  1:1676187/1‑100 (MQ=255)
                                                                     aGGAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGCGAAACGTTCGGTGAAGAAAAAGAAGc                           <  1:1562327/100‑1 (MQ=255)
                                                                      ggAAGTTGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGcc                          >  1:2162766/1‑100 (MQ=255)
                                                                           ttGAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCtt                     <  2:1676175/100‑1 (MQ=255)
                                                                             gAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTg                   >  2:744103/1‑100 (MQ=255)
                                                                             gAACGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTg                   >  1:2045745/1‑100 (MQ=255)
                                                                              aaCGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGa                  <  2:1980244/100‑1 (MQ=255)
                                                                              aaCGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGa                  >  1:1683152/1‑100 (MQ=255)
                                                                                cGTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAg                <  1:1675402/100‑1 (MQ=255)
                                                                                 gTTTTCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAgg               >  1:909783/1‑100 (MQ=255)
                                                                                   tttCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGc             <  1:1170907/100‑1 (MQ=255)
                                                                                    ttCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCa            >  1:95452/1‑100 (MQ=255)
                                                                                     tCTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCat           >  1:199361/1‑100 (MQ=255)
                                                                                      cTGAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCata          <  1:2265073/100‑1 (MQ=255)
                                                                                        gAGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatt        >  2:1208896/1‑100 (MQ=255)
                                                                                         aGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatta       >  2:2381366/1‑100 (MQ=255)
                                                                                         aGCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtatta       >  1:668685/1‑100 (MQ=255)
                                                                                          gCGGTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCAtattat      <  1:1230830/100‑1 (MQ=255)
                                                                                            ggTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc    <  1:1003422/100‑1 (MQ=255)
                                                                                            ggTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc    >  2:501449/1‑100 (MQ=255)
                                                                                            ggTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc    <  2:2318073/100‑1 (MQ=255)
                                                                                            ggTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgc    >  1:760602/1‑100 (MQ=255)
                                                                                             gTCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgct   >  2:1438693/1‑100 (MQ=255)
                                                                                              tCGGGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTAtgctg  >  1:539513/1‑100 (MQ=255)
                                                                                                 |                                                                                                
AAGCTCTGCTTCTGAAAGAAGACGAAATTGTCATTGACCGGAAAAAAATTTCTGCCGACCAGGTTGATCAGGAAGTTGAACGTTTTCTGAGCGGTCGTGCCAAGGCATCAGCCCAGCTGGAAACGATCAAAACGAAAGCTGGTGAAACGTTCGGTGAAGAAAAAGAAGCCATCTTTGAAGGGCATATTATGCTG  >  NC_000913/2534109‑2534302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑