Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,600,288 C→T 100% S128L (TCG→TTG)  gcvR → transcriptional repressor, regulatory protein accessory to GcvA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,600,2880CT98.3% 301.3 / ‑4.2 92S128L (TCG→TTG) gcvRtranscriptional repressor, regulatory protein accessory to GcvA
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (32/58);  total (33/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTCGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAG  >  NC_000913/2600192‑2600382
                                                                                                |                                                                                              
tctgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTtgcg                                                                                             <  1:2247585/100‑1 (MQ=255)
tctgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTtgcg                                                                                             <  2:1898614/100‑1 (MQ=255)
tctgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTtgcg                                                                                             <  2:2028639/100‑1 (MQ=255)
 ctgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTgcgc                                                                                            <  2:1241799/100‑1 (MQ=255)
 ctgGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTgcgc                                                                                            >  2:1631069/1‑100 (MQ=255)
  tgGGTTCAGGTCGATGTGGCAGGCTCCCCGCATTTAATTGAACGCTTCACAGCACGTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgca                                                                                           <  2:522502/100‑1 (MQ=255)
   gggTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcac                                                                                          >  2:1043341/1‑100 (MQ=255)
   gggTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcac                                                                                          >  1:256715/1‑100 (MQ=255)
    ggTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcacg                                                                                         <  1:667401/100‑1 (MQ=255)
     gTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcacgc                                                                                        >  2:2365313/1‑100 (MQ=255)
     gTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGcgcacgc                                                                                        >  2:1178292/1‑100 (MQ=255)
        cAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAAc                                                                                     <  1:744843/100‑1 (MQ=255)
        cAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTCGCGCACGCAAc                                                                                     >  1:2002189/1‑100 (MQ=255)
         aGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAAcc                                                                                    >  1:564076/1‑100 (MQ=255)
         aGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAAcc                                                                                    >  1:2402083/1‑100 (MQ=255)
          ggTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACct                                                                                   <  1:60119/100‑1 (MQ=255)
           gTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGTGCGCAAcacc                                                                                  <  2:1441956/100‑4 (MQ=255)
            tCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACctgc                                                                                 >  2:633432/1‑100 (MQ=255)
            tCGATGTGGCAGACTCCCCGCATTTAATTAAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACctgc                                                                                 <  2:255292/100‑1 (MQ=255)
             cGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACctgct                                                                                <  1:1149400/100‑1 (MQ=255)
             cGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACctgct                                                                                >  1:2186692/1‑100 (MQ=255)
             cGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACctgct                                                                                <  1:1754685/100‑1 (MQ=255)
              ggtgtgGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGTGCGCAAcaccagc                                                                               <  2:853912/98‑7 (MQ=255)
              gATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGTGCGCAAcaccagc                                                                               <  2:138080/100‑7 (MQ=255)
              gATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGTGCGCAAcaccagc                                                                               <  2:138112/100‑7 (MQ=255)
              gATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGTGCGCAAcaccagc                                                                               <  2:1416650/100‑7 (MQ=255)
                tgtgGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGaa                                                                             <  1:720676/100‑1 (MQ=255)
                atctgCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGaa                                                                             <  2:1249640/96‑1 (MQ=255)
                 gtgGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGaaa                                                                            <  1:1757891/100‑1 (MQ=255)
                  tgGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGaaaa                                                                           >  2:2057134/1‑100 (MQ=255)
                  tgGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGaaaa                                                                           >  1:2275571/1‑100 (MQ=255)
                  nggCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGaaa                                                                            >  2:1562871/2‑99 (MQ=255)
                   ggCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGCACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAAt                                                                          <  2:213275/100‑1 (MQ=255)
                   ggCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAAt                                                                          >  1:1392789/1‑100 (MQ=255)
                   ggCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAAt                                                                          <  2:2284508/100‑1 (MQ=255)
                   ggCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAAt                                                                          <  1:632065/100‑1 (MQ=255)
                    gCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGaaagat                                                                         >  1:1249648/1‑97 (MQ=255)
                    gCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATg                                                                         <  1:1981134/100‑1 (MQ=255)
                       gACTCCCCGCATTTAATTGAACGCTTCATAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAAc                                                                      <  1:2492821/100‑1 (MQ=255)
                           ccccGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGc                                                                  <  1:1439251/100‑1 (MQ=255)
                            cccGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCt                                                                 >  1:1482830/1‑100 (MQ=255)
                             ccGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTg                                                                <  1:1159145/100‑1 (MQ=255)
                              cGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTgc                                                               <  1:590156/100‑1 (MQ=255)
                               gCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGAAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTgcg                                                              <  1:1909287/100‑1 (MQ=255)
                                cATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTgcgc                                                             <  2:620473/100‑1 (MQ=255)
                                    tAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGt                                                          >  2:1459017/1‑99 (MQ=255)
                                     aaTTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTCGCGCACGCAACCTGCTGAAAATGAACGGGCTGTGCAGTTg                                                        <  1:1348825/100‑1 (MQ=255)
                                       ttGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCa                                                      <  1:1819976/100‑1 (MQ=255)
                                       ttGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCa                                                      <  2:2447742/100‑1 (MQ=255)
                                       ttGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCa                                                      <  1:2443681/100‑1 (MQ=255)
                                        tGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCat                                                     >  1:529152/1‑100 (MQ=255)
                                         gAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCata                                                    <  2:1648448/100‑1 (MQ=255)
                                         gAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCata                                                    >  2:998423/1‑100 (MQ=255)
                                          aaCGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCatat                                                   >  1:289280/1‑100 (MQ=255)
                                            cGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATAttn                                                 <  2:2515297/100‑2 (MQ=255)
                                              cTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAg                                               >  2:298816/1‑100 (MQ=255)
                                                tCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATCTTCAGAt                                             >  2:1482490/1‑100 (MQ=255)
                                                tCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGAt                                             >  1:1304406/1‑100 (MQ=255)
                                                 cacaGCACTTTTCGCCGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATa                                            >  1:2082621/1‑100 (MQ=255)
                                                   caGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAAc                                          >  1:2039679/1‑100 (MQ=255)
                                                       aCTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGcc                                      <  1:672169/100‑1 (MQ=255)
                                                       aCTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGcc                                      <  1:1406727/100‑1 (MQ=255)
                                                        cTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGccc                                     <  2:1700955/100‑1 (MQ=255)
                                                        cTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGccc                                     <  1:1043291/100‑1 (MQ=255)
                                                         ttttCGACGCGCATCATATGAACATTGCGGAGCTTGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGccc                                     >  2:1235636/1‑99 (MQ=255)
                                                          tttCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCcac                                   <  2:276930/100‑1 (MQ=255)
                                                          tttCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCcac                                   <  2:2561148/100‑1 (MQ=255)
                                                            tCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAg                                 <  1:2426325/100‑1 (MQ=255)
                                                            tCGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAg                                 <  1:1300772/100‑1 (MQ=255)
                                                             cGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGc                                <  2:1748773/100‑1 (MQ=255)
                                                             cGACGCGCATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGc                                >  2:636110/1‑100 (MQ=255)
                                                              gACGCGCTGCATATGAACATTTCGGAGCTTGTGTTGCGCACGCAACCGGCTGAAAATGAACGGGCTGAGCAGATGCATCTTCTGATAACCGCCCACAGcc                               >  2:247083/1‑100 (MQ=255)
                                                                  cgcATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGc                           >  2:2528230/1‑100 (MQ=255)
                                                                   gcATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCa                          <  2:2426744/100‑1 (MQ=255)
                                                                   gcATCATATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCa                          <  2:798751/100‑1 (MQ=255)
                                                                     atcatATGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATc                        <  2:1805005/100‑1 (MQ=255)
                                                                        atatGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGc                     <  2:1482819/100‑1 (MQ=255)
                                                                         tatGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCg                    <  1:1125815/100‑1 (MQ=255)
                                                                         tatGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCg                    <  2:1765954/100‑1 (MQ=255)
                                                                         tatGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCg                    <  1:177657/100‑1 (MQ=255)
                                                                         tatGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCCCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCg                    >  2:1802881/1‑100 (MQ=255)
                                                                          atGAACATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCGCCGCATCTGCgg                   <  1:1688359/100‑1 (MQ=255)
                                                                             aaCATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACg                <  1:1178300/100‑1 (MQ=255)
                                                                               cATTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACgca              <  1:2044156/100‑1 (MQ=255)
                                                                                aTTGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACgcag             >  1:699180/1‑100 (MQ=255)
                                                                                 ttGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACgcagc            >  1:147290/1‑100 (MQ=255)
                                                                                  tGCGGAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACgcagca           >  1:2236175/1‑100 (MQ=255)
                                                                                     ggAGCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAat        <  1:2314966/100‑1 (MQ=255)
                                                                                        gCTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATAtt     <  1:260756/100‑1 (MQ=255)
                                                                                         cTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCCGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTg    <  2:1639329/100‑1 (MQ=255)
                                                                                         cTGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTg    <  1:2459288/100‑1 (MQ=255)
                                                                                          tGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGa   <  1:2118263/100‑1 (MQ=255)
                                                                                          tGGTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGa   <  2:715565/100‑1 (MQ=255)
                                                                                           ggTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAg  <  1:2544079/100‑1 (MQ=255)
                                                                                           ggTGTTGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAg  <  2:1899329/100‑1 (MQ=255)
                                                                                                |                                                                                              
TCTGGGTTCAGGTCGATGTGGCAGACTCCCCGCATTTAATTGAACGCTTCACAGCACTTTTCGACGCGCATCATATGAACATTGCGGAGCTGGTGTCGCGCACGCAACCTGCTGAAAATGAACGGGCTGCGCAGTTGCATATTCAGATAACCGCCCACAGCCCCGCATCTGCGGACGCAGCAAATATTGAG  >  NC_000913/2600192‑2600382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: