Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,716,030 T→G 15.6% intergenic (+20/+36) eamB → / ← grcA cysteine and O‑acetylserine exporter/autonomous glycyl radical cofactor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,716,0300TG15.6% 266.6 / 40.0 114intergenic (+20/+36)eamB/grcAcysteine and O‑acetylserine exporter/autonomous glycyl radical cofactor
Reads supporting (aligned to +/- strand):  ref base T (51/45);  new base G (11/7);  total (62/52)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

TTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTA  >  NC_000913/2715934‑2716129
                                                                                                |                                                                                                   
ttCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGc                                                                                                  >  1:2431083/1‑100 (MQ=255)
  cGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGccc                                                                                                >  1:2316352/1‑100 (MQ=255)
  cGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATATTCTATTAACGAAAAAAAGCGGAAGAGGTCGccc                                                                                                >  2:1343928/1‑100 (MQ=255)
  cGCAAGTATGGTCGCCAGTTAATTATCGTGCTTCCCCTGTTGCTGATCTTTTGCGCGCTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGacc                                                                                                <  1:300778/100‑1 (MQ=255)
    ctaGTATAGTCACCAGTTAAATATCGGACATTCCCAGTAGTTAGTCTATTTCCAAGTAAGCATTTTCTAATAACAAAAAAATGCGGAAGAGTTCg                                                                                                   <  2:231249/93‑1 (MQ=255)
    ccAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAACAGCGGAAGAGGTCCCCctc                                                                                              >  2:2475959/1‑100 (MQ=255)
     cAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCctct                                                                                             <  1:141679/100‑1 (MQ=255)
       gTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTCACGAAAAAAAGCGGAAGAGGTCGCCCTCTTc                                                                                           <  1:618472/100‑1 (MQ=255)
         aTGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGTTGGTCTAGTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGGCCTCTTCCg                                                                                         <  2:1062078/100‑1 (MQ=255)
         aTGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCg                                                                                         <  1:1865392/100‑1 (MQ=255)
         aTGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGACCTCTTCCg                                                                                         <  1:35841/100‑1 (MQ=255)
          tGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGc                                                                                        >  2:2493606/1‑100 (MQ=255)
          tGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGc                                                                                        >  2:1917338/1‑100 (MQ=255)
          tGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGc                                                                                        <  1:1402790/100‑1 (MQ=255)
            gTCGCAAGTTAAATATCGTGCTTGCCATGTTGCTGGTGTATTGCGCGGTACGCATAATAAATAAAAAAAAAAAAGCGGAAGAGGGCGACCTCTTCCGc                                                                                        <  2:1428399/98‑1 (MQ=19)
             tCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTa                                                                                     >  2:665434/1‑100 (MQ=255)
              cGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAg                                                                                    >  2:1816482/1‑100 (MQ=255)
               gCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGt                                                                                   >  1:1756908/1‑100 (MQ=255)
                ccAGTTAAATATCGTGTTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTa                                                                                  <  2:1500317/100‑1 (MQ=255)
                ccAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTa                                                                                  >  2:1807987/1‑100 (MQ=255)
                   gTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACt                                                                               >  1:2189855/1‑100 (MQ=255)
                   gTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGACCTCTTCCGCtttttttcg                                                                               <  1:397866/100‑8 (MQ=37)
                    ttAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACtt                                                                              >  2:364339/1‑100 (MQ=255)
                    ttAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACtt                                                                              <  2:1253993/100‑1 (MQ=255)
                     tAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTg                                                                             >  1:1862183/1‑100 (MQ=255)
                     tAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGACCTCTTCCGCtttttttc                                                                                <  2:2071145/97‑7 (MQ=37)
                      aaaTATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGc                                                                            <  2:1629735/100‑1 (MQ=255)
                       aaTATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCt                                                                           <  1:846463/100‑1 (MQ=255)
                          atCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACt                                                                        >  1:67709/1‑100 (MQ=255)
                           tCGTGCTTGCCCTGTTGCTGGTCTATTGCGAGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGGCGCCCTCTTCCGCTTAGTAACGTGATAc                                                                         <  2:64693/98‑1 (MQ=39)
                               gCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGc                                                                   <  1:1603921/100‑1 (MQ=255)
                                  tGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCtt                                                                >  1:505475/1‑100 (MQ=255)
                                   gCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTa                                                               <  1:66400/100‑1 (MQ=255)
                                    cccTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTAc                                                              >  1:1154861/1‑100 (MQ=255)
                                       tGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAgg                                                           <  2:1111498/100‑1 (MQ=255)
                                        gTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGc                                                          <  1:633453/100‑1 (MQ=255)
                                          tGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCtt                                                        >  2:766956/1‑100 (MQ=255)
                                           gCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCttt                                                       >  2:849298/1‑100 (MQ=255)
                                            cTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTc                                                      <  2:2535685/100‑1 (MQ=255)
                                            cTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTc                                                      >  1:1071159/1‑100 (MQ=255)
                                            cTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTc                                                      >  1:315683/1‑100 (MQ=255)
                                             tgttCTATTGCGCGTTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACGTGCTTCTTAAGCCTTACAGGCTTTCa                                                     <  2:863581/97‑1 (MQ=255)
                                              ggTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCTCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAg                                                    <  2:1368869/100‑1 (MQ=255)
                                              ggTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAg                                                    >  2:725070/1‑100 (MQ=255)
                                              ggTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAg                                                    <  1:2328186/100‑1 (MQ=255)
                                               gTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTGTAAGGCttaagt                                                    <  2:725509/99‑5 (MQ=255)
                                                tCTATTGCGCGGTACGCATTTTCTATTAACGCAACAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAATCCTTACAGGGTTTCa                                                     >  2:1119328/1‑97 (MQ=255)
                                                  tATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTaaa                                                <  2:265455/100‑1 (MQ=255)
                                                  tATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTaaa                                                <  1:1249144/100‑1 (MQ=255)
                                                  tATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTaaa                                                <  1:2438566/100‑1 (MQ=255)
                                                   aTTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAg                                               <  2:1756895/100‑1 (MQ=255)
                                                   aTTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAg                                               >  1:1952414/1‑100 (MQ=255)
                                                    gatctcgGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAgg                                              <  2:2222472/95‑1 (MQ=255)
                                                    gatctcgGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAgg                                              <  2:2189060/95‑1 (MQ=255)
                                                     tGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAgat                                             >  2:1557347/1‑98 (MQ=255)
                                                      gcgcGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTa                                            >  2:2351098/1‑100 (MQ=255)
                                                         cgGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAggggat                                          >  1:2189075/1‑95 (MQ=255)
                                                         cgGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAggagatc                                         >  1:2222486/1‑95 (MQ=255)
                                                          ggTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAg                                        >  2:1943183/1‑100 (MQ=255)
                                                             aCGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGa                                     >  1:1203312/1‑100 (MQ=255)
                                                              cGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGAt                                    >  2:321506/1‑100 (MQ=255)
                                                              cGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGAt                                    >  1:990949/1‑100 (MQ=255)
                                                                   tttCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTCCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGt                               <  2:2385837/100‑1 (MQ=255)
                                                                      cTATTCACGAAAAAAAGCGGAAGATGTCGCTCTCTGCCGCTTATTAACGTGCCACTTAAGCCTTACAAGCTTTCAGTAAAGGTACGAGCGATAACGTctc                            <  2:1772978/100‑3 (MQ=255)
                                                                         ttAACGAAAAGAAGCGGAAGACGTCGCCCTCTTCCGCTTAGTAAATTGCTACTTAACCCTTACAGGCTGTCAGTAACGGTACGAGAGATAACCTCGCgct                         >  2:1409277/1‑100 (MQ=255)
                                                                          tAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgct                         >  2:1464017/1‑99 (MQ=255)
                                                                          gcaagttactaaGCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgctg                        >  2:854365/11‑100 (MQ=37)
                                                                            aCGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgctgct                      <  2:370576/100‑1 (MQ=255)
                                                                            aCGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgctgct                      >  1:1407144/1‑100 (MQ=255)
                                                                            aCGAAAAAAAGCGGAAGAGGTCACCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCgctgct                      >  2:636088/1‑100 (MQ=255)
                                                                             cGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGctgctg                     >  2:1717609/1‑100 (MQ=255)
                                                                              gAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGt                    >  2:2115120/1‑100 (MQ=255)
                                                                                aaaaaaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTc                  >  2:2361804/1‑100 (MQ=255)
                                                                                aaaaaaGCGGAAGAGGTCGCCCTCTCCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTc                  <  2:1195979/100‑1 (MQ=255)
                                                                                 actaaGCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTcc                 >  1:1404469/4‑100 (MQ=38)
                                                                                 aaaaaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTcc                 >  1:1199066/1‑100 (MQ=255)
                                                                                   taaGCGGAAGAAGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGGTcc                 >  2:1834206/2‑98 (MQ=39)
                                                                                   aaaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCgg               <  1:1550257/100‑1 (MQ=255)
                                                                                   aaaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCgg               <  1:134359/100‑1 (MQ=255)
                                                                                   aaaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCgg               <  2:67708/100‑1 (MQ=255)
                                                                                    aaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGa              >  1:1678542/1‑100 (MQ=255)
                                                                                    aaGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGa              <  1:2494315/100‑1 (MQ=255)
                                                                                    aaGCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGCCACGAGCGATAACGTCGCGCTGCTGTTCCgg               >  2:867303/1‑99 (MQ=38)
                                                                                     aGCGGAAGAGTTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGCTATGAGCGATAACGTCGCGCTGCTGTTCCGGAg             <  2:1681139/100‑1 (MQ=255)
                                                                                     aGCGGAAGAGGGCAACCTCTTCCGCCTAGTCACTTGCTAGTTAAGACATGAAGGCGTTAAGTCAAGGTACGAGAGGTAACGTCGCGCTGCTGTTCCg                >  2:1068227/1‑97 (MQ=255)
                                                                                      gCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGt            <  1:1394367/100‑1 (MQ=255)
                                                                                      gCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTCAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGCTCCggc              >  2:1964101/1‑97 (MQ=39)
                                                                                      gCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGt            >  1:1163229/1‑100 (MQ=255)
                                                                                      gCGGAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGt            >  1:1097955/1‑100 (MQ=255)
                                                                                       cGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTc           >  2:300203/1‑100 (MQ=255)
                                                                                        ggAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAATGTACGGGCGATAACGTCGCGCTGCTGTTCCGGAGTCa          >  2:1724448/1‑100 (MQ=255)
                                                                                        ggAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGGGTCa          >  2:845801/1‑100 (MQ=255)
                                                                                        ggAAGAGGGCGACCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCa          >  2:1543069/1‑100 (MQ=255)
                                                                                         gAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCag         <  2:1328511/100‑1 (MQ=255)
                                                                                           agagGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCagag       >  2:643783/1‑100 (MQ=255)
                                                                                           agagGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCagag       <  1:2288238/100‑1 (MQ=255)
                                                                                             agGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCagagag     >  1:525170/1‑100 (MQ=255)
                                                                                              ggTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGt    >  1:603530/1‑100 (MQ=255)
                                                                                              ggTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGt    >  1:1483280/1‑100 (MQ=255)
                                                                                                tCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGCGCGATAACGTCGCCCTGCTGTTACGGAGTCAGAGAGTTa  >  2:394898/1‑100 (MQ=255)
                                                                                                |                                                                                                   
TTCGCCAGTATGGTCGCCAGTTAAATATCGTGCTTGCCCTGTTGCTGGTCTATTGCGCGGTACGCATTTTCTATTAACGAAAAAAAGCGGAAGAGGTCGCCCTCTTCCGCTTAGTAACTTGCTACTTAAGCCTTACAGGCTTTCAGTAAAGGTACGAGCGATAACGTCGCGCTGCTGTTCCGGAGTCAGAGAGTTA  >  NC_000913/2715934‑2716129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: