Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,772,237 A→G 16.8% pseudogene (830/900 nt) yfjV ← pseudogene, CP4‑57 prophage;Phage or Prophage Related; putative pump protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,772,2370AG16.8% 253.7 / 41.3 113pseudogene (830/900 nt)yfjVpseudogene, CP4‑57 prophage;Phage or Prophage Related; putative pump protein
Reads supporting (aligned to +/- strand):  ref base A (46/48);  new base G (9/10);  total (55/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 114 total aligned reads displayed.

TGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTT  >  NC_000913/2772139‑2772336
                                                                                                  |                                                                                                   
tGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAAt                                                                                                    >  2:2125187/1‑100 (MQ=255)
     aTGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCgg                                                                                               <  1:1267976/100‑1 (MQ=255)
     aTGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCgg                                                                                               <  2:822912/100‑1 (MQ=255)
       gTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTa                                                                                             >  2:2104398/1‑100 (MQ=255)
        ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGCGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTAc                                                                                            <  2:1460742/100‑1 (MQ=255)
        ttGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgc                                                                                            <  2:1476285/100‑3 (MQ=25)
        ctGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgc                                                                                            <  2:2049118/99‑3 (MQ=25)
         tGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgct                                                                                           <  1:1171512/100‑4 (MQ=25)
         tGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgct                                                                                           <  2:489831/100‑4 (MQ=25)
          gCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgctg                                                                                          <  1:1389681/100‑5 (MQ=25)
          gCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgctg                                                                                          <  1:657193/100‑5 (MQ=25)
          gCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgcag                                                                                          >  1:2049132/1‑96 (MQ=25)
          gCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAAAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACgg                                                                                          >  2:1850398/1‑100 (MQ=255)
             cATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgctgttt                                                                                       <  1:694019/100‑8 (MQ=25)
             cATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAg                                                                                       <  1:1089472/100‑1 (MQ=255)
             cATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAg                                                                                       >  2:1908026/1‑100 (MQ=255)
             cATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAg                                                                                       >  1:1441867/1‑100 (MQ=255)
              atatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCATTGTCATGACTGTGCCGGtgctgtttg                                                                                      <  1:319670/100‑9 (MQ=25)
               tatCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTa                                                                                     >  2:2199703/1‑100 (MQ=255)
                atCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTaa                                                                                    >  2:1887470/1‑100 (MQ=255)
                atCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTaa                                                                                    <  1:1410087/100‑1 (MQ=255)
                  cAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAata                                                                                  >  1:2317038/1‑100 (MQ=255)
                  cAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAata                                                                                  <  1:1101772/100‑1 (MQ=255)
                  cAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGACCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAata                                                                                  <  2:337756/100‑1 (MQ=255)
                   aGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAata                                                                                  <  2:49616/99‑1 (MQ=255)
                    gTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATc                                                                                >  2:2453056/1‑100 (MQ=255)
                      aTCTACTTACAAAGTGACATACAGCCGCCCCGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATccc                                                                              >  2:39006/1‑100 (MQ=37)
                       tCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATcccc                                                                             <  1:745447/100‑1 (MQ=255)
                        cTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCATCGGCACAGTCATGACAATGCCGGCACTGAAGTAATATCCCCa                                                                            <  2:1729460/100‑1 (MQ=255)
                        cTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCa                                                                            <  1:1485030/100‑1 (MQ=255)
                         tAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCACCaa                                                                           >  2:1170426/1‑100 (MQ=255)
                           aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGt                                                                         >  2:562188/1‑100 (MQ=255)
                           aTTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGt                                                                         <  2:2040674/100‑1 (MQ=255)
                            ttACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTg                                                                        <  1:1780791/100‑1 (MQ=255)
                             tACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGa                                                                       >  1:365781/1‑100 (MQ=255)
                             tACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGa                                                                       >  2:2111228/1‑100 (MQ=255)
                             tACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGa                                                                       <  1:1304110/100‑1 (MQ=255)
                             tACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGa                                                                       >  1:1208405/1‑100 (MQ=255)
                              aCAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAt                                                                      <  1:149796/100‑1 (MQ=255)
                              aCAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAt                                                                      >  1:1871251/1‑100 (MQ=255)
                               cAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGAtt                                                                     >  2:519675/1‑100 (MQ=255)
                                   gTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCa                                                                 >  2:320192/1‑100 (MQ=255)
                                      aCAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCatat                                                              >  1:307396/1‑100 (MQ=255)
                                        agagagCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttt                                                            >  1:1627577/1‑100 (MQ=255)
                                         gagagCCGCCACGCGAGCGGGGCCAGAGTACTAAATAGCACCGTCACAGTAATGATAATGCCGGTACGGAAGTAATATCCCCGAGTGATTGTCATAtttt                                                           <  2:1521556/100‑1 (MQ=25)
                                         gagagCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAtttt                                                           >  1:407079/1‑100 (MQ=255)
                                           gagCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATTACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTc                                                         <  2:845935/100‑1 (MQ=255)
                                           gagCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATAttttta                                                         >  2:1046151/1‑99 (MQ=255)
                                           gagCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTc                                                         <  1:1506338/100‑1 (MQ=255)
                                           gagCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTc                                                         >  2:242348/1‑100 (MQ=255)
                                            agCCGCCACGCCAGCGCGGCCAGATTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTc                                                         <  2:884671/99‑1 (MQ=255)
                                            agCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCt                                                        <  2:86931/100‑1 (MQ=255)
                                             gccgccACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTg                                                       <  1:1743251/100‑1 (MQ=255)
                                             gccgccACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTg                                                       >  2:2151209/1‑100 (MQ=255)
                                              ccgccACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTgg                                                      <  1:1511768/100‑1 (MQ=255)
                                                gccacgccaGCGCGGCCAGAGTGACAAACAGCAGCGGCACAGTCATGACAATGCCGGTACGGAAGAAATATCCCCAAGTGATTGTCATATTTTTCTggg                                                     <  2:1113092/99‑1 (MQ=255)
                                                gccacgccaGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGc                                                    <  1:679659/100‑1 (MQ=255)
                                                 ccacgccaGCGCGGCCAGAGTGACAAACAGCACCGTCACAGTCATTACAATGCCGGTACGTAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGc                                                    <  2:2326336/99‑1 (MQ=255)
                                                 ccacgccaGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCa                                                   >  1:1887062/1‑100 (MQ=255)
                                                  cacgccaGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTggtc                                                    >  2:301075/1‑96 (MQ=255)
                                                  cacgccaGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCaa                                                  >  1:632537/1‑100 (MQ=255)
                                                    cgccaGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGc                                                <  1:1785095/100‑1 (MQ=255)
                                                    cgccaGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGc                                                <  1:767430/100‑1 (MQ=255)
                                                     gccaGCGCGGCCAGAGTGACAAATAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGca                                               >  1:1021103/1‑100 (MQ=255)
                                                     gccaGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGca                                               <  2:2317024/100‑1 (MQ=255)
                                                         gcgcGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATg                                           <  2:307392/100‑1 (MQ=255)
                                                           gcgGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCa                                         <  2:257380/100‑1 (MQ=255)
                                                              gCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGcc                                      <  2:2149908/100‑1 (MQ=255)
                                                              gCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGcc                                      >  1:2348395/1‑100 (MQ=255)
                                                               ccAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCca                                     >  1:127181/1‑100 (MQ=255)
                                                                      gACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCAcagcag                               <  2:729188/99‑1 (MQ=255)
                                                                      gACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCaga                              >  1:729195/1‑100 (MQ=255)
                                                                      gACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCaga                              <  2:365777/100‑1 (MQ=255)
                                                                      gACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCaga                              >  1:451230/1‑100 (MQ=255)
                                                                       aCAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCagag                             <  2:434398/100‑1 (MQ=255)
                                                                        cAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCaga                              >  2:60123/1‑98 (MQ=255)
                                                                          ttccgtaCCGGCATTGTCATGACGGGGCCGGTACGGAAGTCATATCTACAAGTGATTGTCATATTTTTCTGGGCAAGCCCCAGCAGCCACCACAGAGTTg                          >  2:2331291/7‑100 (MQ=16)
                                                                            cAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGcc                        <  1:202865/100‑1 (MQ=255)
                                                                             cgtaCCGGCATTGTCATGACTGTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCa                       >  1:191525/4‑100 (MQ=38)
                                                                             cgtaCCGGCATTGTCATGACTGTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTGTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCa                       >  1:1800888/4‑100 (MQ=37)
                                                                              gtaCCGGCATTGTCATGACTGTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAg                      >  2:274545/3‑100 (MQ=38)
                                                                              gtaCCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAg                      >  1:1608214/3‑100 (MQ=255)
                                                                                aCCGGCATTGTCATGACTGTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGAc                    >  2:2203749/1‑100 (MQ=38)
                                                                                aCCGGCATTGTCATGACTGTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGAc                    >  1:2114409/1‑100 (MQ=38)
                                                                                 ccggcattgTCATGACTGTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACt                   >  1:2057550/9‑100 (MQ=38)
                                                                                  cGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTg                  <  1:2135036/100‑1 (MQ=255)
                                                                                  cGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTg                  <  2:787437/100‑1 (MQ=255)
                                                                                  cGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTg                  <  1:2135075/100‑1 (MQ=255)
                                                                                   ggCACAGTCATGACAATGCCTGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTTGGCAAGCACATGCAGCCACAGCAGGGTTGCCAGACTGc                 <  2:1582430/100‑1 (MQ=255)
                                                                                   ggCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACttc                 <  1:1730325/100‑3 (MQ=255)
                                                                                   ggCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGc                 <  1:2151223/100‑1 (MQ=255)
                                                                                    gCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGcc                <  1:1957238/100‑1 (MQ=255)
                                                                                     cacaGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCa               >  1:394352/1‑100 (MQ=255)
                                                                                     cacaGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCa               <  2:1761620/100‑1 (MQ=255)
                                                                                          tCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAATCgg          >  1:253314/1‑100 (MQ=255)
                                                                                           cATGACTGTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAATCggg         <  2:191523/100‑1 (MQ=255)
                                                                                           cATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAATCgg          >  2:1844359/1‑99 (MQ=255)
                                                                                                cAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGAt    >  1:687169/1‑100 (MQ=255)
                                                                                                cAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGAt    >  1:689269/1‑100 (MQ=255)
                                                                                                  aTGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGAGGTGAttt  >  2:766506/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
TGGTAATGTTGCTCATATCAGTATCTAATTACAAAGTGACAGAGAGCCGCCACGCCAGCGCGGCCAGAGTGACAAACAGCACCGGCACAGTCATGACAATGCCGGTACGGAAGTAATATCCCCAAGTGATTGTCATATTTTTCTGGGCAAGCACATGCAGCCACAGCAGAGTTGCCAGACTGCCAATCGGGGTGATTT  >  NC_000913/2772139‑2772336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: