Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,807,095 G→A 18.1% intergenic (+21/‑37) proW → / → proX glycine betaine/proline ABC transporter permease/glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,807,0950GA18.1% 221.0 / 39.0 104intergenic (+21/‑37)proW/proXglycine betaine/proline ABC transporter permease/glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
Reads supporting (aligned to +/- strand):  ref base G (43/42);  new base A (2/17);  total (45/59)
Fisher's exact test for biased strand distribution p-value = 1.61e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

Only 100 of 104 total aligned reads displayed.

ACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGC  >  NC_000913/2806998‑2807193
                                                                                                 |                                                                                                  
aCTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGcc                                                                                                  >  2:2447253/1‑100 (MQ=255)
  tCACGCAGTCGCGGCAACCGTCGCTGGTAGACTGATGGCCCTGTTGGTCTGCTGACCCGGCCATTCATTAAGTCCCTCTGCACTTGCCCGGTGACGc                                                                                                   >  2:828718/1‑97 (MQ=255)
   cACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCggg                                                                                               >  2:1844868/1‑100 (MQ=255)
   cACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTAACCCGCCCATCCATTAAGTACCTCTGCACTTGCCCGGTGACGCCggg                                                                                               <  2:1584030/100‑1 (MQ=255)
     cGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCa                                                                                             <  1:797899/100‑1 (MQ=255)
     cGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCa                                                                                             <  2:1777856/100‑1 (MQ=255)
     cGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCa                                                                                             <  1:2211292/100‑1 (MQ=255)
     cGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCa                                                                                             <  2:1283271/100‑1 (MQ=255)
      gCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCAt                                                                                            >  2:1284637/1‑100 (MQ=255)
      gCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCAt                                                                                            >  1:2106587/1‑100 (MQ=255)
       ctgTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTGGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaag                                                                                           <  2:1007526/98‑3 (MQ=255)
       cAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaag                                                                                           <  1:2549721/100‑3 (MQ=255)
       cAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaag                                                                                           <  1:410224/100‑3 (MQ=255)
       cAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaag                                                                                           <  1:459905/100‑3 (MQ=255)
       cAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaag                                                                                           <  1:1129956/100‑3 (MQ=255)
       cAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaag                                                                                           <  2:806933/100‑3 (MQ=255)
       cAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaag                                                                                           <  1:1368457/100‑3 (MQ=255)
        aGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTa                                                                                          <  1:614889/100‑1 (MQ=255)
           cgcgGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCa                                                                                       <  1:552051/100‑1 (MQ=255)
           cgcgGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaagtgca                                                                                       <  1:2456679/100‑7 (MQ=255)
            gcgGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCAc                                                                                      <  1:1997023/100‑1 (MQ=255)
            gcgGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCAc                                                                                      <  2:603845/100‑1 (MQ=255)
             cgGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGACGCCGGGCATTATCAcc                                                                                     >  1:2411450/1‑100 (MQ=255)
              ggCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGTCCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaagtgcagag                                                                                    <  1:113216/100‑10 (MQ=37)
              ggCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaagtgcagag                                                                                    <  1:790049/100‑10 (MQ=38)
              ggCAACCGTCGCTGGTACACCACTGGCCCTGTTGGCCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaagtgcagag                                                                                    <  1:2276296/100‑10 (MQ=37)
                 aaCCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGc                                                                                 <  1:1809105/100‑1 (MQ=255)
                    cGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCaa                                                                              >  1:1338213/1‑100 (MQ=255)
                        gCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCaaaaaa                                                                          >  2:2203341/1‑100 (MQ=255)
                          tagTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGTCATTATCACCAAAACAAAAAAAg                                                                        <  2:1993006/98‑1 (MQ=255)
                          tGGTACACCACTGGCCCTGTTTGTCTGCTGACCCGCCCATTCATTACGTAACTCTCCACTTGCCCGTTGACGCCGGGCATTATCACCCTGCCaaa                                                                             <  2:1057083/95‑1 (MQ=255)
                          tGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAg                                                                        <  1:616678/100‑1 (MQ=255)
                          tGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAg                                                                        <  2:1078120/100‑1 (MQ=255)
                           ngTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAgg                                                                       >  2:1948361/2‑100 (MQ=255)
                           ggTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAgg                                                                       <  1:1090288/100‑1 (MQ=255)
                               caccacTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATa                                                                   >  1:2529817/1‑100 (MQ=255)
                               caccacTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATa                                                                   >  1:2221509/1‑100 (MQ=255)
                                accacTGGCCCTGTTGGTCTGCTGACCCGACCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATaa                                                                  <  1:2163110/100‑1 (MQ=255)
                                 ccacTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAAc                                                                 >  2:1805676/1‑100 (MQ=255)
                                 ccacTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAAc                                                                 >  2:504674/1‑100 (MQ=255)
                                 ccacTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAAc                                                                 >  1:806919/1‑100 (MQ=255)
                                 ccacTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAAc                                                                 <  1:1380786/100‑1 (MQ=255)
                                      ggCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGc                                                            >  2:1716563/1‑100 (MQ=255)
                                      ggCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATAACCCTAAAAAAAAAAGGAATAACAATGc                                                            <  2:806181/100‑1 (MQ=255)
                                        cccTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGa                                                          <  1:2148526/100‑1 (MQ=255)
                                         ccTGTTGGTCTGGTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCAAAATAACAAAAAAAGGAATAACAATGCGAc                                                         <  2:2544356/100‑1 (MQ=255)
                                         ccTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGAc                                                         <  1:2057513/100‑1 (MQ=255)
                                           tGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACAt                                                       <  1:907604/100‑1 (MQ=255)
                                              tGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGc                                                    >  2:859690/1‑100 (MQ=255)
                                               ggTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTCTCACCCAGCCAAAAAAAGGAATAACAATGCGACATAGCg                                                   >  2:1801707/1‑100 (MQ=255)
                                                gTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGt                                                  <  1:737841/100‑1 (MQ=255)
                                                 tCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACGTAGCGTa                                                 <  2:1706026/100‑1 (MQ=255)
                                                  ctgctgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTAc                                                <  1:614429/100‑1 (MQ=255)
                                                  ctgctgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTAc                                                >  2:1952851/1‑100 (MQ=255)
                                                   tgctgACCCGCCCATTCATTAAGTAACTCTGCACTTTCCCGGTGAAGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTAc                                                >  2:513357/1‑99 (MQ=255)
                                                   tgctgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACt                                               >  2:1763654/1‑100 (MQ=255)
                                                   tgctgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCCGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACt                                               <  1:1200003/100‑1 (MQ=255)
                                                    gctgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACtt                                              >  1:1889211/1‑100 (MQ=255)
                                                       gACCCGCCCATTCATTAAGTAACTCTGCACTAGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACttttt                                           >  2:2465033/1‑100 (MQ=255)
                                                        aCCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTg                                          >  2:1321691/1‑100 (MQ=255)
                                                         cccGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGc                                         >  2:1018302/1‑100 (MQ=255)
                                                           cGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCg                                        <  2:1133230/99‑1 (MQ=255)
                                                            gCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAATAGGAATAACAATGCGACATAGCGTACTTTTTGCGAc                                      <  1:1801721/100‑1 (MQ=255)
                                                            gCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGAc                                      <  1:2054458/100‑1 (MQ=255)
                                                            gCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGAc                                      <  2:498036/100‑1 (MQ=255)
                                                            gCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGAc                                      >  1:1031723/1‑100 (MQ=255)
                                                              ccATTCATTAATTAACTCTGCACTTGCTCGGTGACAACAGGCATTATGACCATGCCAAAAATATGAATAACATTACGATAATGCGTAATTTTTGCAACa                                     >  2:483604/1‑99 (MQ=255)
                                                                 ttcattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGt                                 >  1:489557/1‑100 (MQ=255)
                                                                      tAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGcc                            <  1:2328935/100‑1 (MQ=255)
                                                                       aaGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCa                           >  1:1763189/1‑100 (MQ=255)
                                                                          tAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGACACGc                        >  2:1020749/1‑100 (MQ=255)
                                                                           aaCTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCt                       <  1:2303592/100‑1 (MQ=255)
                                                                           aaCTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCt                       <  1:1530955/100‑1 (MQ=255)
                                                                           aaCTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCt                       >  2:730113/1‑100 (MQ=255)
                                                                           aaCTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCt                       <  2:483477/100‑1 (MQ=255)
                                                                           aaCTCTGCACTTACCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCt                       >  2:2533901/1‑100 (MQ=255)
                                                                             ctctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTa                     >  1:2092206/1‑100 (MQ=255)
                                                                             ctctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTa                     <  1:1940689/100‑1 (MQ=255)
                                                                              tctGCACTTGCCGGGTGACGCAGGGCATTATTACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTa                     <  2:2388798/99‑1 (MQ=255)
                                                                              tctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTAt                    >  1:2153612/1‑100 (MQ=255)
                                                                              tctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTAt                    >  1:515837/1‑100 (MQ=255)
                                                                              tctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTAt                    >  1:112508/1‑100 (MQ=255)
                                                                              tctGCACTTGCCCGGTGACGCCGGGCAGTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTa                     <  2:2411436/99‑1 (MQ=255)
                                                                               ctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTAtc                   >  1:2167736/1‑100 (MQ=255)
                                                                               ctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTAtc                   <  1:599637/100‑1 (MQ=255)
                                                                               ctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTAtc                   <  2:650148/100‑1 (MQ=255)
                                                                                tgataatGCCCGGCGTCACCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTAtct                  >  2:609487/7‑100 (MQ=255)
                                                                                 gCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTAtctc                 <  1:2186273/100‑1 (MQ=255)
                                                                                   aCTTGCCCGGTGGCGCCGGGCATTATCACCCTACCAGAAAAAGGAATAACCATGCGACACAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTCTCTCTa               >  2:2491170/1‑100 (MQ=255)
                                                                                     ttGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTaca             <  2:489552/100‑1 (MQ=255)
                                                                                      tGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTacac            >  2:2286257/1‑100 (MQ=255)
                                                                                       gCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGTGTTAGTGTTTGCCACGCTTATCTCTAcaca           >  2:157567/1‑100 (MQ=255)
                                                                                       gCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTAcaca           <  1:805570/100‑1 (MQ=255)
                                                                                        cccGGTGACGCCGAGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACaa          >  2:1210500/1‑100 (MQ=255)
                                                                                         ncGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACaaa         >  1:72073/2‑100 (MQ=255)
                                                                                           ggTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACt       >  2:1516439/1‑100 (MQ=255)
                                                                                           ggTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCCACAGCGTTTGCCACGCTTATCTCTACACAAACt       <  2:1692453/100‑1 (MQ=255)
                                                                                            gTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACtt      >  2:2411423/1‑100 (MQ=255)
                                                                                             tGACGCCGGGCATTATCACCCTGCCAAAAAAAGTAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACttt     <  1:297093/100‑1 (MQ=255)
                                                                                                cGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtgc  >  2:2553985/1‑100 (MQ=255)
                                                                                                 |                                                                                                  
ACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGC  >  NC_000913/2806998‑2807193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: