BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,150,120	T→C	100%	F153F (TTT→TTC)	yghA →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,150,120	0	T	C	98.2%	366.8 / ‑2.9	108	F153F (TTT→TTC)	yghA	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (42/64); total (43/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

Only 100 of 109 total aligned reads displayed.

AAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTC  >  NC_000913/3150023‑3150218

aaGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCa                                                                                                  <  1:1313533/100‑1 (MQ=255)

  gcgcACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCaaa                                                                                                <  1:838866/100‑1 (MQ=255)

   cgcACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCaaaa                                                                                               >  1:2544000/1‑100 (MQ=255)

     cacaAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGa                                                                                             >  2:1114733/1‑100 (MQ=255)

           gCGTTAGGCGGGCTGGATATTATGGCGCTGGGCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTg                                                                                       <  2:1717795/100‑1 (MQ=255)

            cGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGc                                                                                      >  1:1053647/1‑100 (MQ=255)

            cGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGc                                                                                      <  2:1403571/100‑1 (MQ=255)

            cGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGc                                                                                      >  1:1252641/1‑100 (MQ=255)

             gTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGcc                                                                                     >  2:1628209/1‑100 (MQ=255)

               tAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCAt                                                                                   >  1:2307036/1‑100 (MQ=255)

               tAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCAt                                                                                   >  2:2276300/1‑100 (MQ=255)

                 ggCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTa                                                                                 >  2:275600/1‑100 (MQ=255)

                 ggCGGGCTGGATATTACGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCACAAGACCTTTGCCATTa                                                                                 >  2:476907/1‑100 (MQ=255)

                  gCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTaa                                                                                <  2:1720668/100‑1 (MQ=255)

                    gggCTGGATATTATGGCGCTGGTCGCCGGGAAAGAGGTTGCTCTTCCGGATATTGCAGACCTCAACAGCGAACAGTTCAAAAAGACCTTTGCAATTAACg                                                                              >  2:1046656/1‑100 (MQ=255)

                        tGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGtttt                                                                          >  2:1127048/1‑100 (MQ=255)

                         ggATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTc                                                                         >  2:2525641/1‑100 (MQ=255)

                         ggATATTATGGCGCTGGTCGCCGGGAAACAGGGTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTc                                                                         >  2:2514113/1‑100 (MQ=255)

                           atatTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTcgc                                                                       <  1:1883119/100‑1 (MQ=255)

                           atatTATGGCGCTGGTCGCCGGGAAACAGGGTGACATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTGACGTTTTcgc                                                                       >  2:1685371/1‑100 (MQ=255)

                            tattatGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTcgcg                                                                      <  2:1294778/100‑1 (MQ=255)

                               tatGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTg                                                                   <  1:1466376/100‑1 (MQ=255)

                               tatGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTg                                                                   <  1:23388/100‑1 (MQ=255)

                                 tGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGtt                                                                 >  1:1463324/1‑100 (MQ=255)

                                   gcgcTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCt                                                               <  2:685094/100‑1 (MQ=255)

                                   gcgcTGGTCGACGGGAAACAGGTTGCCGTTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAACAGTCCTTTGCCATTAACGTTTTCGCGCTGTTCt                                                               <  2:1683263/100‑1 (MQ=255)

                                     gcTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTgg                                                             <  2:489996/100‑1 (MQ=255)

                                     gcTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTgg                                                             <  1:1296722/100‑1 (MQ=255)

                                      cTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGc                                                            <  1:651812/100‑1 (MQ=255)

                                      cTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGc                                                            <  2:18751/100‑1 (MQ=255)

                                      cTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGc                                                            <  1:1498597/100‑1 (MQ=255)

                                       tGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCt                                                           <  2:1776047/100‑1 (MQ=255)

                                       tGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCAATTAACGTTTTCGCGCTGTTCTGGCt                                                           <  1:1216993/100‑1 (MQ=255)

                                        ggTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTa                                                          >  2:829366/1‑100 (MQ=255)

                                        ggTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTa                                                          <  2:735444/100‑1 (MQ=255)

                                         gTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTaa                                                         >  2:2251333/1‑100 (MQ=255)

                                           cGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAAcc                                                       <  1:794880/100‑1 (MQ=255)

                                           cGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAAcc                                                       <  2:525190/100‑1 (MQ=255)

                                            gCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAAccc                                                      >  2:1519461/1‑100 (MQ=255)

                                             ccGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCa                                                     <  1:2074408/100‑1 (MQ=255)

                                              cGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAg                                                    <  1:329050/100‑1 (MQ=255)

                                              cGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAg                                                    <  1:275335/100‑1 (MQ=255)

                                              cGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAg                                                    <  1:512817/100‑1 (MQ=255)

                                                 gAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGaa                                                 >  1:476383/1‑100 (MQ=255)

                                                   aaCAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGc                                               <  1:1169510/100‑1 (MQ=255)

                                                   aaCAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGc                                               <  1:90651/100‑1 (MQ=255)

                                                    aCAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCg                                              <  1:983235/100‑1 (MQ=255)

                                                    aCAGGTTGCCATTCCGGATATTGAAGACCTCACCGGCGAAAAGTTCCAAAAGAACTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCTAGGAAGCg                                              <  1:1114741/100‑1 (MQ=255)

                                                     cAGGTTGCCATTCCGGATATTGCATACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGa                                             <  2:2526740/100‑1 (MQ=255)

                                                     cAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGa                                             <  1:1655609/100‑1 (MQ=255)

                                                      aGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGAt                                            <  1:2435755/100‑1 (MQ=255)

                                                      aGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGAt                                            <  1:1004592/100‑1 (MQ=255)

                                                        gTTGCCATTCCGGATATTG‑AGACCTCACCAGTGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATcn                                          <  2:1799026/99‑2 (MQ=255)

                                                         ttGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATccc                                         <  2:2186313/100‑1 (MQ=255)

                                                         ttGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCACTGTTCTGGCTAACGCAGGAAGCGATccc                                         >  1:443403/1‑100 (MQ=255)

                                                          tGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATcccc                                        >  1:359805/1‑100 (MQ=255)

                                                           gCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTCTCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATccccc                                       <  2:430137/100‑1 (MQ=255)

                                                            ccATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCt                                      >  1:384459/1‑100 (MQ=255)

                                                             cATTCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTg                                     <  2:1766450/100‑1 (MQ=255)

                                                               ttCCGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCt                                   >  2:2287846/1‑100 (MQ=255)

                                                                tCCGGAAATTCCCCACCTCACCAGCGAACAGTTCCAAATGACCTTCGCCATTAACGGTTTCGCGCGGGTCTGGCTGACGCAGGAAGCGATGCTCCTGCt                                   >  2:2394385/1‑99 (MQ=255)

                                                                 ccGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTAc                                 >  2:2499371/1‑100 (MQ=255)

                                                                  cGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTAcc                                <  2:1785135/100‑1 (MQ=255)

                                                                  cGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTAcc                                <  2:1822609/100‑1 (MQ=255)

                                                                  cGGATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTAcc                                >  1:2223540/1‑100 (MQ=255)

                                                                   ggATATTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCg                               <  1:1166569/100‑1 (MQ=255)

                                                                   ggATATTGCAGACCTCACCAGCGAACAGTGCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTAcc                                <  2:699476/99‑1 (MQ=255)

                                                                      tatTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGaaa                            <  1:829620/100‑1 (MQ=255)

                                                                       atTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGaaa                            <  1:1449011/99‑1 (MQ=255)

                                                                       atTGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAg                           <  2:1926600/100‑1 (MQ=255)

                                                                        ttGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAgg                          <  1:2422547/100‑1 (MQ=255)

                                                                        ttGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAgg                          >  1:424504/1‑100 (MQ=255)

                                                                        ttGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAgg                          <  1:630629/100‑1 (MQ=255)

                                                                         tGCAGACCTCACTAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACTCAGGAGGCGGTCCCCGTGCTACCGAAAgg                          >  2:844251/1‑99 (MQ=255)

                                                                         tGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGt                         <  1:943997/100‑1 (MQ=255)

                                                                         tGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAgg                          >  2:1175841/1‑99 (MQ=255)

                                                                         tGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGt                         >  2:1114041/1‑100 (MQ=255)

                                                                         tGCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGt                         >  2:691520/1‑100 (MQ=255)

                                                                          gCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTg                        >  1:2096919/1‑100 (MQ=255)

                                                                          gCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTg                        <  2:532273/100‑1 (MQ=255)

                                                                          gCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTg                        >  1:1386281/1‑100 (MQ=255)

                                                                          gCAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTg                        >  1:364987/1‑100 (MQ=255)

                                                                           cAGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGc                       <  1:2233114/100‑1 (MQ=255)

                                                                            aGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCa                      <  1:2333895/100‑1 (MQ=255)

                                                                            aGACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCa                      <  2:1489091/100‑1 (MQ=255)

                                                                             gACCTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCaa                     >  2:187089/1‑100 (MQ=255)

                                                                               ccTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAg                    <  2:413216/99‑1 (MQ=255)

                                                                               ccTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGt                   >  1:2544258/1‑100 (MQ=255)

                                                                               ccTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGt                   <  2:2026677/100‑1 (MQ=255)

                                                                               ccTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGt                   <  2:189568/100‑1 (MQ=255)

                                                                                cTCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTa                  >  1:2031532/1‑100 (MQ=255)

                                                                                 tCACCAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTat                 >  2:2347008/1‑100 (MQ=255)

                                                                                     cAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc             <  1:275604/100‑1 (MQ=255)

                                                                                     cAGCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTatcatc             <  1:907878/100‑1 (MQ=255)

                                                                                       gCGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTAtcatca            <  2:1693467/99‑1 (MQ=255)

                                                                                        cGAACAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATcacn          <  1:1253094/100‑2 (MQ=255)

                                                                                            cAGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACtt      >  1:1456570/1‑100 (MQ=255)

                                                                                             aGTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTc     <  2:1659341/100‑1 (MQ=255)

                                                                                              gTTCCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCg    <  1:1133367/100‑1 (MQ=255)

                                                                                                ttCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTc  <  1:1073323/100‑1 (MQ=255)

AAGCGCACAAGGCGTTAGGCGGGCTGGATATTATGGCGCTGGTCGCCGGGAAACAGGTTGCCATTCCGGATATTGCAGACCTCACCAGCGAACAGTTTCAAAAGACCTTTGCCATTAACGTTTTCGCGCTGTTCTGGCTAACCCAGGAAGCGATCCCCCTGCTACCGAAAGGTGCAAGTATCATCACCACTTCGTC  >  NC_000913/3150023‑3150218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`