Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,165,015	T→A	100%	D320V (GAT→GTT)	parC ←	DNA topoisomerase IV, subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,165,015	0	T	A	99.4%	305.2 / ‑6.3	98	D320V (GAT→GTT)	parC	DNA topoisomerase IV, subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (59/38);  minor base C (1/0);  total (60/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

ACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAT  >  NC_000913/3164919‑3165110                                                                                                 |                                                                                                acCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCTCCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACgg                                                                                              <  1:1799277/100‑1 (MQ=255) acCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACgg                                                                                              <  1:2165808/100‑1 (MQ=255)   cAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACGATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtg                                                                                            >  2:2342146/1‑100 (MQ=255)   cAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtg                                                                                            >  1:1391619/1‑100 (MQ=255)   cAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtg                                                                                            <  1:1505911/100‑1 (MQ=255)    aGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtgg                                                                                           <  2:757175/100‑1 (MQ=255)     gCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtggt                                                                                          <  1:680959/100‑1 (MQ=255)     gCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtggt                                                                                          <  2:49139/100‑1 (MQ=255)     gCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACggtggt                                                                                          <  1:787831/100‑1 (MQ=255)       cATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAg                                                                                        >  2:2070569/1‑100 (MQ=255)        aTTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGc                                                                                       <  2:1486605/100‑1 (MQ=255)        aTTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGc                                                                                       <  2:801933/100‑1 (MQ=255)         ttCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCg                                                                                      <  2:964436/100‑1 (MQ=255)          tCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGa                                                                                     <  2:2450499/100‑1 (MQ=255)            ggAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAag                                                                                   >  1:1657613/1‑100 (MQ=255)            ggAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAag                                                                                   <  1:821334/100‑1 (MQ=255)             gagaGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAaga                                                                                  >  1:1425810/1‑100 (MQ=255)               gagGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAgg                                                                                <  1:58934/100‑1 (MQ=255)                agGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggt                                                                               <  1:761630/100‑1 (MQ=255)                 ggATTTCCAGCAGGTTTTTCACCGCTGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGCGGCAGc                                                                                       >  2:861947/1‑91 (MQ=255)                 ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtg                                                                              >  1:1221199/1‑100 (MQ=255)                 ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtg                                                                              >  1:2264820/1‑100 (MQ=255)                 ggATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATAATATTACGGTTAATACGATAGCTCTTTTCCAGACCGGTGCTAGCAAAGAggt                                                                               >  1:2138435/1‑99 (MQ=255)                   aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtggt                                                                            >  1:331859/1‑100 (MQ=255)                   aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtggt                                                                            <  2:1430010/100‑1 (MQ=255)                   aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAggtggt                                                                            >  1:2023315/1‑100 (MQ=255)                    tttCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGtt                                                                           >  2:1945337/1‑100 (MQ=255)                      tCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATAGTAAGGTTAATACGCTAGCTCCTTTCCAGAACGGTGGTAACGAagag                                                                                 >  2:2051195/1‑92 (MQ=255)                       ccAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcat                                                                        >  1:1248975/1‑100 (MQ=255)                         agcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcatca                                                                      >  1:33653/1‑100 (MQ=255)                         agcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTtcatc                                                                       >  1:2134843/1‑99 (MQ=255)                         agcagGTTTTTCACCGCAGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCAAGAACGGTGGTAGCGAAGAGGTGGTtcataa                                                                      >  2:1650231/1‑98 (MQ=255)                          gcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAc                                                                     >  2:1164172/1‑100 (MQ=255)                          gcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAc                                                                     >  1:272048/1‑100 (MQ=255)                           cagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCAcc                                                                    <  1:1219220/100‑1 (MQ=255)                             ggTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTg                                                                  >  1:72315/1‑100 (MQ=255)                             ggTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTg                                                                  >  2:576336/1‑100 (MQ=255)                             ggTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTg                                                                  >  2:871359/1‑100 (MQ=255)                              gTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCACAACGTTGGTACCGAAGAGGTGGGTCATCACCTGa                                                                 >  2:2313727/1‑100 (MQ=255)                               tttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGAt                                                                >  1:2549253/1‑100 (MQ=255)                               tttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGGTAATACGATAGCTCTTTTCCAGAACGCTGGTCGCGCAGAGGTGGTTCCTCAACtca                                                                 >  2:1323217/1‑97 (MQ=255)                                ttttCACCTCCTGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATc                                                               >  2:2156077/1‑100 (MQ=255)                                  ttCACCGCCGGACGACCATCCATACCGATCATATTAAGGCTCATACGATAGCTCTTTCCCAGAACGGAGGTAGCGAACAGGTGGTACATCCCCCGa                                                                 >  2:2215656/1‑96 (MQ=255)                                    caccggcaaacgacCACCCAGACCCATCATATTAAGGTTAATACGAGAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATcc                                                              <  2:1883243/88‑1 (MQ=255)                                     aCCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTGTTTTCCAGAACGGTGGTAGCGCAGAGGTGGTTCATCACCTGAACCatat                                                          >  2:1691199/1‑100 (MQ=255)                                      ccgccgGACGAACATCCAGACCGATCATATTAAGGTTAAGACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGCGGTGGTGCATCGCCTGATCCATATc                                                         >  2:473418/1‑100 (MQ=255)                                       cgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATcc                                                        >  1:8876/1‑100 (MQ=255)                                       cgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATcc                                                        <  1:696176/100‑1 (MQ=255)                                         ccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCAc                                                      <  2:2550633/100‑1 (MQ=255)                                         ccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCAc                                                      >  1:1869643/1‑100 (MQ=255)                                           ggACGACCATCCAGACCGATCATATTAAGGTTAAGACGAGAGCTCTTGTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCAcgc                                                    <  2:2146390/100‑1 (MQ=255)                                              cgacCATCCAGACCGATCATATTAAGGTTAACACGATAGCTCTTTTCCAGAACGGTGGTAGCGAACAGCACGTCCATCACCTGATCCATATCCCCGCgg                                                  >  2:1595362/1‑99 (MQ=255)                                                acCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTg                                               >  1:613752/1‑100 (MQ=255)                                                 ccATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTgg                                              >  2:2111270/1‑100 (MQ=255)                                                  cATCCAGACCGATCATATTAAGGTTAATACGATAGCTTTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGa                                             >  2:1363849/1‑100 (MQ=255)                                                  cATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGa                                             >  1:1663057/1‑100 (MQ=255)                                                  cATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGa                                             >  2:2004936/1‑100 (MQ=255)                                                   aTCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGaa                                            >  1:1992679/1‑100 (MQ=255)                                                    tCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAc                                           <  1:81746/100‑1 (MQ=255)                                                      cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAcgc                                         >  2:8619/1‑100 (MQ=255)                                                      cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAAcg                                          >  2:1471284/1‑99 (MQ=255)                                                        gtccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCgg                                       <  2:1463808/98‑1 (MQ=255)                                                         aCCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGc                                      <  2:2084612/100‑1 (MQ=255)                                                          ccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGca                                     <  2:1854860/100‑1 (MQ=255)                                                          ccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGca                                     <  1:2315809/100‑1 (MQ=255)                                                            gATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGcaca                                   >  2:415924/1‑100 (MQ=255)                                                               cATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCATCTGATCCATATCCACGCGGTTGGAACGCGGCACAATc                                <  2:1376282/100‑1 (MQ=255)                                                               cATATTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATc                                <  2:270101/100‑1 (MQ=255)                                                                atatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCa                               <  2:591588/100‑1 (MQ=255)                                                                 tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                              >  1:935018/1‑100 (MQ=255)                                                                 tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                              >  2:1195639/1‑100 (MQ=255)                                                                 tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCAc                              >  2:1861656/1‑100 (MQ=255)                                                                 tatTAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATAGCCACGCGGTTGGAACGCGGCACAATCAc                              >  2:2043322/1‑100 (MQ=255)                                                                   ttAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCa                            <  1:1307450/100‑1 (MQ=255)                                                                    tAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAg                           >  2:2499669/1‑100 (MQ=255)                                                                    tAAGGTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACAAg                           >  2:580975/1‑100 (MQ=255)                                                                       ggTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcg                        >  1:2271610/1‑100 (MQ=255)                                                                       ggTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcg                        >  1:738879/1‑100 (MQ=255)                                                                       ggTTAATACGATAGCTCTTGTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcg                        <  2:2256769/100‑1 (MQ=255)                                                                        gTTAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgg                       >  2:1872592/1‑100 (MQ=255)                                                                        gTTAATACGATAGCTCTTTTACAGAACGGTGGTAGCGAAGAGGTGGTTCATCACTTGATCCATATACACGCGGTTGGAACGCGGGACAATCACCAGGcgg                       >  2:1124018/1‑100 (MQ=255)                                                                         ttAATACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcggg                      <  2:879515/100‑1 (MQ=255)                                                                            aTACGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcg                   >  2:1302470/1‑100 (MQ=255)                                                                              aCGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcggg                 <  1:1861670/100‑1 (MQ=255)                                                                              aCGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggccggg                 >  2:395480/1‑100 (MQ=255)                                                                               cGATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggtcgggt                <  1:335378/100‑1 (MQ=255)                                                                                gATAGCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGcgggccgggt                >  1:763558/1‑99 (MQ=255)                                                                                    gCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCg           <  1:871367/100‑1 (MQ=255)                                                                                    gCTCTTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCg           >  1:891283/1‑100 (MQ=255)                                                                                     ctctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGt          >  1:1630329/1‑100 (MQ=255)                                                                                      tctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTg         <  2:2409896/100‑1 (MQ=255)                                                                                       ctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTgg        >  1:1425034/1‑100 (MQ=255)                                                                                       ctTTTCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTgg        <  2:1571020/100‑1 (MQ=255)                                                                                        ttttCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGt       <  1:899585/100‑1 (MQ=255)                                                                                           tCCAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAg    >  2:973158/1‑100 (MQ=255)                                                                                            ccAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGa   <  1:1447032/100‑1 (MQ=255)                                                                                             cAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCGCAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAt  >  2:808483/1‑100 (MQ=255)                                                                                             cAGAACGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAt  >  2:271065/1‑100 (MQ=255)                                                                                                 |                                                                                                ACCAGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCACGCGGTTGGAACGCGGCACAATCACCAGGCGGGTCGGGTTCTCGTGGTCAGAT  >  NC_000913/3164919‑3165110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑