Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,171,857 A→T 15.5% intergenic (+24/+22) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,171,8570AT15.5% 332.1 / 47.1 128intergenic (+24/+22)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (65/43);  new base T (11/9);  total (76/52)
Fisher's exact test for biased strand distribution p-value = 8.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.44e-01

Only 100 of 134 total aligned reads displayed.

CGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATA  >  NC_000913/3171758‑3171953
                                                                                                   |                                                                                                
cGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTa                                                                                                  >  2:980437/1‑100 (MQ=255)
 gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaa                                                                                                 >  1:1002275/1‑100 (MQ=255)
 gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaa                                                                                                 >  2:2307487/1‑100 (MQ=255)
 gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaa                                                                                                 <  2:83770/100‑1 (MQ=255)
 gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaa                                                                                                 >  1:1431050/1‑100 (MQ=255)
 gCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTaa                                                                                                 >  1:160202/1‑100 (MQ=255)
     aaTTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAAc                                                                                              <  2:2143705/99‑1 (MQ=255)
        tGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGAct                                                                                          <  2:1961685/100‑1 (MQ=255)
          catggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGActct                                                                                        >  1:2401495/1‑100 (MQ=255)
          catggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGActct                                                                                        <  2:1479530/100‑1 (MQ=255)
          catggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGActct                                                                                        >  1:1554228/1‑100 (MQ=255)
          catggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGActct                                                                                        >  1:1554134/1‑100 (MQ=255)
          catggcatgAATGTTGAATTTGGGAATGCGGAACAAGCTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGActct                                                                                        >  2:1364635/1‑100 (MQ=255)
            tggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCa                                                                                      <  2:1938317/100‑1 (MQ=255)
             ggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCAt                                                                                     <  2:1163366/100‑1 (MQ=38)
             ggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCAt                                                                                     >  1:568435/1‑100 (MQ=255)
             ggcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCAt                                                                                     <  1:354125/100‑1 (MQ=255)
              gcatgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATa                                                                                    <  2:1436259/100‑1 (MQ=38)
               catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAg                                                                                   <  1:788369/100‑1 (MQ=38)
               catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAg                                                                                   >  2:2382755/1‑100 (MQ=255)
               catgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAg                                                                                   >  1:2175068/1‑100 (MQ=255)
                atgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGTCGTTTTAACGGCTCTCATAGa                                                                                  <  1:491072/100‑1 (MQ=38)
                  gAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGAtt                                                                                <  1:746444/100‑1 (MQ=255)
                    aTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGATTCTCATAGAtttt                                                                              <  1:1115190/100‑1 (MQ=255)
                      gTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTAc                                                                            >  1:2134829/1‑100 (MQ=255)
                       ttGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACt                                                                           <  1:2048746/100‑1 (MQ=255)
                       ttGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACt                                                                           <  1:1856543/100‑1 (MQ=255)
                         gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                         >  2:1651894/1‑100 (MQ=255)
                            tttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTGACGTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATa                                                                       >  2:919501/1‑99 (MQ=255)
                              tGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                    >  1:2297625/1‑100 (MQ=255)
                                 gAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaaaa                                                                 <  2:2160593/100‑1 (MQ=255)
                                  aaTGCGGAACAAGGTGGGTTTGAGGCGAAGATAAGCTGGTAACTTCTGTGTCTATGAGAGCCGTTAAAACGACGCTCATAGATTTTACTAATAGCaaaa                                                                 <  2:2386098/99‑1 (MQ=255)
                                   aTGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAata                                                               >  1:116184/1‑100 (MQ=255)
                                     gCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAAtata                                                             >  1:836810/1‑100 (MQ=255)
                                      cGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATaa                                                            >  2:1740211/1‑100 (MQ=255)
                                          aCAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCg                                                        >  2:232821/1‑100 (MQ=255)
                                          aCAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCg                                                        >  1:2361758/1‑100 (MQ=255)
                                          aCAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCg                                                        >  1:1518230/1‑100 (MQ=255)
                                             aGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCTAAATATAAACCGTcc                                                     >  1:2518619/1‑100 (MQ=255)
                                              ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTccc                                                    >  1:1454917/1‑100 (MQ=255)
                                                 ggATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaa                                                 >  2:1770015/1‑100 (MQ=255)
                                                     ttGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCaaaaaa                                             >  1:1782932/1‑100 (MQ=255)
                                                       gAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAg                                            <  1:2447413/99‑1 (MQ=255)
                                                       gAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGc                                           <  1:978485/100‑1 (MQ=255)
                                                        aGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcc                                          >  2:744139/1‑100 (MQ=255)
                                                         ggCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca                                         >  2:1132880/1‑100 (MQ=255)
                                                         ggCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca                                         >  1:957126/1‑100 (MQ=255)
                                                              aGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaac                                    >  1:1044946/1‑100 (MQ=255)
                                                               ggTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacc                                   <  1:2441758/100‑1 (MQ=255)
                                                               ggTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacc                                   <  2:1304245/100‑1 (MQ=255)
                                                                gTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacca                                  >  1:711463/1‑100 (MQ=255)
                                                                gTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacca                                  >  1:566647/1‑100 (MQ=255)
                                                                 tAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                 >  2:81980/1‑100 (MQ=255)
                                                                  aaGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcaca                                <  2:227427/100‑1 (MQ=255)
                                                                    gCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACaaa                              >  2:1484962/1‑100 (MQ=255)
                                                                    gCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACaaa                              >  1:2075727/1‑100 (MQ=255)
                                                                    gCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACaaa                              >  2:2561039/1‑100 (MQ=255)
                                                                    gCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACAGTCACCAAACAAGCCACCACCCACaaa                              <  2:161525/100‑1 (MQ=255)
                                                                     cTGGTAACTTCTCTGTCTATGAGAGCCTTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                             <  2:1214388/100‑1 (MQ=255)
                                                                      tGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAcc                            >  1:280481/1‑100 (MQ=255)
                                                                          aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAc                        >  1:847355/1‑100 (MQ=255)
                                                                          aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAc                        <  2:1804486/100‑1 (MQ=255)
                                                                          aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGAc                        <  1:2175301/100‑1 (MQ=255)
                                                                           aCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACa                       <  2:1014338/100‑1 (MQ=255)
                                                                              tctcTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAAt                    <  1:350093/100‑1 (MQ=255)
                                                                              tctcTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAAt                    >  2:176060/1‑100 (MQ=255)
                                                                               tcttGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATa                   <  1:1965474/97‑1 (MQ=255)
                                                                                tctGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATAc                  >  1:800519/1‑100 (MQ=255)
                                                                                  tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATaaga                >  2:1965460/1‑97 (MQ=255)
                                                                                  tGTCTATGAGAACCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATaaga                >  2:1967623/1‑97 (MQ=255)
                                                                                    tCTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag              >  1:1033964/1‑100 (MQ=38)
                                                                                    tCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag              <  1:1386550/100‑1 (MQ=255)
                                                                                    tCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAACAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag              >  1:1097754/1‑100 (MQ=255)
                                                                                     cTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc             >  1:1824572/1‑100 (MQ=38)
                                                                                     cTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc             >  1:1691144/1‑100 (MQ=38)
                                                                                     cTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc             >  1:822604/1‑100 (MQ=38)
                                                                                     cTATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga               >  2:852089/1‑98 (MQ=38)
                                                                                     cTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc             >  2:1296925/1‑100 (MQ=255)
                                                                                     cTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc             >  1:386343/1‑100 (MQ=255)
                                                                                      tATGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGCCCCCAAAAAAGCCACCAACCACAAACCACACAAAAACctagc             >  2:719675/1‑99 (MQ=37)
                                                                                      tATGAGAGTCGTTTTAACGGCTCTCATAGATCTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcga               >  2:1956655/1‑97 (MQ=38)
                                                                                      tATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg            <  1:1917757/100‑1 (MQ=255)
                                                                                      tATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag              >  2:1820707/1‑98 (MQ=255)
                                                                                      tATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACAAACCACAAACCAGACAAATACcgagcg            >  2:1471913/1‑100 (MQ=255)
                                                                                      tATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGACACCAACCACAAACCAGACAAATACcgagcg            >  2:1735692/1‑100 (MQ=255)
                                                                                       aTGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga           >  1:1341330/1‑100 (MQ=38)
                                                                                       aTGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcga           >  2:268571/1‑100 (MQ=38)
                                                                                       aTGAGAGTCGTTTTAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag              >  2:1397442/1‑97 (MQ=38)
                                                                                         gaggGCCGTTAAAACGACTCTGATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCgaga         <  1:2412755/100‑1 (MQ=255)
                                                                                         gagaGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAAACAAATACCGAGCgaga         >  2:2093492/1‑100 (MQ=255)
                                                                                          agagCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa        >  1:684287/1‑100 (MQ=255)
                                                                                            agCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATa      <  2:1504043/100‑1 (MQ=255)
                                                                                            agCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATa      <  2:571766/100‑1 (MQ=255)
                                                                                             gCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATAc     >  1:1499743/1‑100 (MQ=255)
                                                                                             gCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGCCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATAc     >  2:355132/1‑100 (MQ=255)
                                                                                              ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACa    <  1:2057142/100‑1 (MQ=255)
                                                                                              ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACa    <  1:2307501/100‑1 (MQ=255)
                                                                                                gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata  >  2:2238154/1‑100 (MQ=255)
                                                                                                gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata  >  1:924072/1‑100 (MQ=255)
                                                                                                 ttATAACAACACTCATACTTTTTACAAAACGCAAAATATAAACCTTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata  <  2:244158/99‑1 (MQ=255)
                                                                                                   |                                                                                                
CGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATA  >  NC_000913/3171758‑3171953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: