Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,202,003 G→C 18.3% G38A (GGC→GCC)  cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,202,0030GC18.3% 221.5 / 41.9 87G38A (GGC→GCC) ccafused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
Reads supporting (aligned to +/- strand):  ref base G (39/32);  new base C (14/2);  total (53/34)
Fisher's exact test for biased strand distribution p-value = 2.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

TGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTA  >  NC_000913/3201904‑3202102
                                                                                                   |                                                                                                   
tGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCggg                                                                                                     >  1:618448/1‑100 (MQ=255)
      gtggtgCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTAcc                                                                                                <  2:1232617/99‑1 (MQ=255)
         gtgCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACcagca                                                                                            <  1:244098/100‑1 (MQ=255)
         gtgCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACcagca                                                                                            <  2:263307/100‑1 (MQ=255)
          tgCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACcagcag                                                                                           >  1:2152249/1‑100 (MQ=255)
           gCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAAACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAgg                                                                                          >  1:1807775/1‑100 (MQ=255)
             tGTTCGGGATGCATTGTTAGAGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGCGATGCTCGACGCGGGCTACCAGAAGGTa                                                                                        >  2:981572/1‑100 (MQ=255)
              gTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAg                                                                                       >  1:2488289/1‑100 (MQ=255)
              gTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAg                                                                                       >  2:334834/1‑100 (MQ=255)
                tCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGc                                                                                     <  2:568825/100‑1 (MQ=255)
                 cGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGcc                                                                                    <  2:338773/100‑1 (MQ=255)
                   ggATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCcgc                                                                                  <  2:984835/100‑1 (MQ=255)
                    gATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCcgcg                                                                                 >  1:1461403/1‑100 (MQ=255)
                      tGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGAt                                                                               <  1:296686/100‑1 (MQ=255)
                      tGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGAt                                                                               <  1:2491928/100‑1 (MQ=255)
                      tGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGCCTACCTGCTGGTAGCCcgcgtc                                                                               <  2:2557758/100‑3 (MQ=38)
                      tGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGGGGTCGGCAGTACGCCACAGGAGATGTTCGACGCGGGCTACCAGCAGGTAGGCCGCGAt                                                                               <  2:538141/100‑1 (MQ=255)
                        ttccgatctggCTACCGGTCAATGACAGAGACTGGGTCGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGAttt                                                                             <  2:1727315/91‑1 (MQ=255)
                         aTTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGAtttt                                                                            >  1:2402652/1‑100 (MQ=255)
                           tGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGTCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTcc                                                                          <  2:1804369/100‑1 (MQ=255)
                           tGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTcc                                                                          >  1:1804383/1‑100 (MQ=255)
                           tGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTcc                                                                          <  2:1767734/100‑1 (MQ=255)
                           tGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTcc                                                                          <  2:618441/100‑1 (MQ=255)
                            gTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCt                                                                         >  2:2384975/1‑100 (MQ=255)
                              tAGGGCTACCGGTCAAAGACAGAGATTGGGTGTTGGTCGGCAGTACGCCACACGAGATGCACGACGCGGCCTACCAGCAGCTCGCCCGCGAt                                                                               >  2:1976215/1‑92 (MQ=255)
                                 ggCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGtt                                                                    >  1:1888540/1‑100 (MQ=255)
                                  gCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGttt                                                                   >  2:1065744/1‑100 (MQ=255)
                                     aCCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTg                                                                <  1:835308/100‑1 (MQ=255)
                                     aCCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTg                                                                <  1:59037/100‑1 (MQ=255)
                                        ggTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCAt                                                             >  1:1071788/1‑100 (MQ=255)
                                         gTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATc                                                            <  2:67138/100‑1 (MQ=255)
                                          tCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATcc                                                           <  2:262404/100‑1 (MQ=255)
                                           cAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCg                                                          >  2:2110798/1‑100 (MQ=255)
                                               gACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCaaa                                                      >  2:2501331/1‑100 (MQ=255)
                                               gACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCaaa                                                      <  1:1065752/100‑1 (MQ=255)
                                               gACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCaaa                                                      >  2:1097426/1‑100 (MQ=255)
                                                 cAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACg                                                    >  1:2312845/1‑100 (MQ=255)
                                                   gagaTTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCa                                                  >  2:361131/1‑100 (MQ=255)
                                                   gagaTTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCa                                                  <  1:918031/100‑1 (MQ=255)
                                                     gaTTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATg                                                >  1:42796/1‑100 (MQ=255)
                                                     gaTTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATg                                                <  2:1468461/100‑1 (MQ=255)
                                                      aTTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGa                                               <  1:334838/100‑1 (MQ=255)
                                                        tGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAag                                             >  2:582680/1‑100 (MQ=255)
                                                         gggTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAaga                                            >  1:2117578/1‑100 (MQ=255)
                                                           gtggtggtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGt                                          >  2:1378647/1‑100 (MQ=255)
                                                            tggtggtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTa                                         <  2:776917/100‑1 (MQ=255)
                                                            tggtggtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTa                                         <  1:767398/100‑1 (MQ=255)
                                                               tggtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATgc                                      <  1:2183148/100‑1 (MQ=255)
                                                                ggtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATgcg                                     >  2:200492/1‑100 (MQ=255)
                                                                ggtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATgc                                      >  2:1475785/1‑99 (MQ=255)
                                                                 gtCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATgcgc                                    <  2:2038532/100‑1 (MQ=255)
                                                                  tCGGCAGTACGCCACATGAGATGCTCGACGCGCGCTACCAGCAGGTAGGCCGCGATTTTCCTCTCTTTCTTCATCCTCAAACGCATGAAGAGTATGCGCt                                   <  2:2389746/100‑1 (MQ=255)
                                                                   cGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTACAGTGTTTCTGCATCCGCAAACGAATGAAGAGTAttagct                                   >  2:744157/1‑94 (MQ=255)
                                                                    ggCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTgg                                 <  2:111763/100‑1 (MQ=255)
                                                                    ggCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTgg                                 >  1:306833/1‑100 (MQ=255)
                                                                        gTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACg                             >  2:1461721/1‑100 (MQ=255)
                                                                           cGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTAc                          >  1:682664/1‑100 (MQ=255)
                                                                            gCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTAcc                         >  1:657859/1‑100 (MQ=255)
                                                                             ccACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCg                        >  1:680250/1‑100 (MQ=255)
                                                                             ccACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCg                        >  1:1554959/1‑100 (MQ=255)
                                                                                  ggAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACgg                   >  1:1349686/1‑100 (MQ=255)
                                                                                  ggAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACgg                   >  2:1047495/1‑100 (MQ=255)
                                                                                   gagaTGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGa                  <  1:35459/100‑1 (MQ=255)
                                                                                     caggaaaatcgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACTGATGAAGAGTATGCGCTGGCACGTACCGAACGGaaa                >  2:1829955/10‑100 (MQ=21)
                                                                                      aTGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAt               <  1:200494/100‑1 (MQ=255)
                                                                                       tGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtc              >  2:444108/1‑100 (MQ=255)
                                                                                       tGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtc              >  2:1967776/1‑100 (MQ=255)
                                                                                       ggaaaatcgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtc              >  2:2098419/8‑100 (MQ=25)
                                                                                        gcgggACGCGGGCTACCAGCAGGTAGGCCGCGATGTTCCTGGGTGTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAgcc             <  2:1701667/96‑1 (MQ=255)
                                                                                         cTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccg            >  1:1451101/1‑100 (MQ=255)
                                                                                         aaaatcgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccg            >  2:549786/6‑100 (MQ=37)
                                                                                         aaaatcgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccg            >  1:1428768/6‑100 (MQ=37)
                                                                                         aaaatcgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccg            >  1:826403/6‑100 (MQ=37)
                                                                                          tCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTTTTTTTTTTTTTCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccgg           >  2:2484657/1‑100 (MQ=255)
                                                                                          aaatcgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccgg           >  2:710129/5‑100 (MQ=37)
                                                                                           cGACGTGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggt          <  2:2038222/100‑1 (MQ=255)
                                                                                           aatcgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggt          >  2:523151/4‑100 (MQ=38)
                                                                                           aatcgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggt          >  1:2002893/4‑100 (MQ=38)
                                                                                           aatcgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggt          <  1:549793/97‑1 (MQ=38)
                                                                                            atcgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTATCGAAAGGAAAtccgg           >  2:858532/3‑98 (MQ=37)
                                                                                            atcgcgGCCTACCTGCTGGTAGCCCGCGACTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggtt         >  2:1030840/3‑100 (MQ=38)
                                                                                             tcgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttc        >  1:62632/2‑100 (MQ=38)
                                                                                              cgcgGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCCTGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttcc       >  1:995253/1‑100 (MQ=255)
                                                                                              cgcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttcc       >  1:2183517/1‑100 (MQ=38)
                                                                                               gcgGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttccg      <  2:51748/100‑1 (MQ=255)
                                                                                               gcgGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttccg      >  1:1359134/1‑100 (MQ=255)
                                                                                               gcgGCCTACCTGCTGGTAGCCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttccg      >  1:1204819/1‑100 (MQ=255)
                                                                                                cgGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttccgg     >  2:1845567/1‑100 (MQ=255)
                                                                                                 gggCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttccggt    <  1:1311117/100‑1 (MQ=255)
                                                                                                  ggCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATccggttccggtt   <  1:1633852/100‑1 (MQ=255)
                                                                                                   gCTCCCAGCAGTTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAAtccggttccgg     <  1:1616036/97‑1 (MQ=255)
                                                                                                   gCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTa  >  2:128747/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
TGGTCGGTGGTGCTGTTCGGGATGCATTGTTAGGGCTACCGGTCAAAGACAGAGATTGGGTGGTGGTCGGCAGTACGCCACAGGAGATGCTCGACGCGGGCTACCAGCAGGTAGGCCGCGATTTTCCTGTGTTTCTGCATCCGCAAACGCATGAAGAGTATGCGCTGGCACGTACCGAACGGAAATCCGGTTCCGGTTA  >  NC_000913/3201904‑3202102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: