Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,350,648 T→A 100% intergenic (‑189/+41) elbB ← / ← arcB isoprenoid biosynthesis protein with amidotransferase‑like domain/aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,350,6480TA96.2% 340.3 / 1.9 105intergenic (‑189/+41)elbB/arcBisoprenoid biosynthesis protein with amidotransferase‑like domain/aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base A (49/52);  total (52/53)
Fisher's exact test for biased strand distribution p-value = 3.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 105 total aligned reads displayed.

ATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCC  >  NC_000913/3350552‑3350744
                                                                                                |                                                                                                 
aTTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGgt                                                                                                >  1:721353/1‑100 (MQ=255)
aTTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGgt                                                                                                >  1:745525/1‑100 (MQ=255)
aTTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGgt                                                                                                >  2:1105702/1‑100 (MQ=255)
 tttAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGgtg                                                                                               <  1:1039817/100‑1 (MQ=255)
 tttAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGgtg                                                                                               >  2:422054/1‑100 (MQ=255)
  ttAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGgtgt                                                                                              <  1:226507/100‑1 (MQ=255)
  ttAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCATGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGgtgt                                                                                              <  2:2303939/100‑1 (MQ=255)
     aGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTgg                                                                                           <  2:378610/100‑1 (MQ=255)
     aGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTgg                                                                                           >  2:2447316/1‑100 (MQ=255)
        tttGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGgcgc                                                                                        <  1:2056722/100‑1 (MQ=255)
        tttGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGgcgc                                                                                        <  1:1388293/100‑1 (MQ=255)
         ttGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCa                                                                                       <  1:582359/100‑1 (MQ=255)
         ttGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCa                                                                                       <  1:1881092/100‑1 (MQ=255)
         ttGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCa                                                                                       <  1:171104/100‑1 (MQ=255)
          tGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAg                                                                                      <  1:882415/100‑1 (MQ=255)
           gCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGt                                                                                     >  1:406879/1‑100 (MQ=255)
             tATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTAt                                                                                   >  2:1608054/1‑100 (MQ=255)
               tgtttatagcgtgcgGCCTTAAAAATAGTGCTTTTCCCTTGTAACTCACCCTATTAATTTACGGCTCAGCCCCGCTTTCCCAAGTGTTGGCGCAGTa                                                                                    <  2:2078812/89‑1 (MQ=255)
               tCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTc                                                                                 >  1:1751074/1‑100 (MQ=255)
                  tAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTcgcg                                                                              <  1:469597/100‑1 (MQ=255)
                   aaCTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCgcgc                                                                             <  1:498/100‑1 (MQ=255)
                      tgcgtgcgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCAcc                                                                          <  2:1504591/100‑1 (MQ=255)
                       gcgtgcgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCAccc                                                                         >  1:1098846/1‑100 (MQ=255)
                       gcgtgcgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCAccc                                                                         >  1:1693179/1‑100 (MQ=255)
                        cgtgcgGACTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCAcccc                                                                        >  1:1340704/1‑100 (MQ=255)
                           gcgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCACGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGt                                                                     >  2:1817470/1‑100 (MQ=255)
                              gCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTa                                                                  >  1:1193688/1‑100 (MQ=255)
                              gCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTa                                                                  >  1:2262338/1‑100 (MQ=255)
                               ccTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAg                                                                 <  1:1653981/100‑1 (MQ=255)
                                cTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGc                                                               >  2:2520642/1‑100 (MQ=255)
                                cTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGATGTTGGCGCAGTATTCGCGCACCCCGGTCTAGc                                                               <  2:1693174/100‑1 (MQ=255)
                                cTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGc                                                               <  1:102446/100‑1 (MQ=255)
                                cTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGc                                                               >  2:1840458/1‑100 (MQ=255)
                                 tGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGcc                                                              >  2:830177/1‑100 (MQ=255)
                                  gAAAAACAGTGCTGTGCCCTTGTAACTCATCATCATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCg                                                             >  2:1688541/1‑100 (MQ=255)
                                  gAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCg                                                             >  1:1403253/1‑100 (MQ=255)
                                  gAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCg                                                             >  1:980977/1‑100 (MQ=255)
                                   aaaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGcc                                                              <  2:1551082/98‑1 (MQ=255)
                                   aaaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCgg                                                            <  1:993011/100‑1 (MQ=255)
                                   aaaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCg                                                             <  2:1642040/99‑1 (MQ=255)
                                   aaaaaCAGTGCTGTGCCCTTGTAACTCATCAGAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTGGGCGCAGTATTCGCGCACCCCGGTCTAGCCgg                                                            <  2:637819/100‑1 (MQ=255)
                                    aaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCggg                                                           >  2:2411221/1‑100 (MQ=255)
                                    aaaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCggg                                                           <  1:2076104/100‑1 (MQ=255)
                                     aaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCgggg                                                          >  1:370848/1‑100 (MQ=255)
                                     aaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCgggg                                                          <  1:453146/100‑1 (MQ=255)
                                     aaaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCgggg                                                          >  1:1469535/1‑100 (MQ=255)
                                      aaCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGt                                                         <  1:2180570/100‑1 (MQ=255)
                                       aCAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTc                                                        >  1:717008/1‑100 (MQ=255)
                                        cAGTGCTGTGCCCTTGTAACTCATCAGAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGTCTAGACCGGGGtg                                                        <  2:1587011/99‑2 (MQ=255)
                                         aGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGCTAGACCGGGGtgcg                                                      <  1:1767827/100‑4 (MQ=255)
                                         aGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGCTAGACCGGGGtgcg                                                      <  1:1379255/100‑4 (MQ=255)
                                           tGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGCTAGACCGGGGtgcgcg                                                    <  1:1608065/100‑6 (MQ=255)
                                              atctcccTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCAtttttt                                                 <  2:554092/96‑1 (MQ=255)
                                               gtgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTa                                                >  2:1649372/1‑100 (MQ=255)
                                               gtgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTa                                                >  2:1942951/1‑100 (MQ=255)
                                                tgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAg                                               <  1:488997/100‑1 (MQ=255)
                                                tgCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAg                                               <  2:1403242/100‑1 (MQ=255)
                                                  cccTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAgat                                             >  1:554099/1‑98 (MQ=255)
                                                  cccTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTg                                             >  1:1471504/1‑100 (MQ=255)
                                                        tAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCtttt                                       <  1:544555/100‑1 (MQ=255)
                                                          aCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGc                                     <  2:804737/100‑1 (MQ=255)
                                                          aCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGc                                     <  1:26401/100‑1 (MQ=255)
                                                          aCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGc                                     >  2:1872419/1‑100 (MQ=255)
                                                          aCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGc                                     <  1:1942965/100‑1 (MQ=255)
                                                           cTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCtttagat                                    >  2:348790/1‑96 (MQ=255)
                                                           cTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGcc                                    <  1:224275/100‑1 (MQ=255)
                                                           cTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGAAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTg                                      <  2:191632/98‑1 (MQ=255)
                                                             catcatAATAATTTACGGCGCAGCCAAGCTTTCCCAGGTGTTGGCGCAGTATTCGCGCACACCGGTCTAGCCGGGGACATTGTTTAGTGGCTTTTGcc                                    >  2:1298925/1‑98 (MQ=255)
                                                              atcatAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccacc                                 <  2:1132308/100‑1 (MQ=255)
                                                              atcatAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccacc                                 >  2:1087233/1‑100 (MQ=255)
                                                               tcatAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccaccc                                >  2:505123/1‑100 (MQ=255)
                                                               tcatAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccaccc                                >  2:965608/1‑100 (MQ=255)
                                                               tcatAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccaccc                                >  1:1782511/1‑100 (MQ=255)
                                                                catAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCTCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccaccca                               <  1:620063/100‑1 (MQ=255)
                                                                catAATAATTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccaccc                                >  2:1503343/1‑99 (MQ=255)
                                                                  taataaTTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACg                             >  1:2437860/1‑100 (MQ=255)
                                                                  taataaTTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACg                             >  1:1818716/1‑100 (MQ=255)
                                                                  taataaTTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACg                             >  2:1613761/1‑100 (MQ=255)
                                                                   aataatTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGc                            >  1:1366462/1‑100 (MQ=255)
                                                                    ataatTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCt                           >  1:2005174/1‑100 (MQ=255)
                                                                    ataatTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCt                           >  1:1460874/1‑100 (MQ=255)
                                                                    ataatTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCt                           >  2:2436909/1‑100 (MQ=255)
                                                                       atTTGCGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTc                        <  1:210218/100‑1 (MQ=255)
                                                                       atTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTc                        <  2:643411/100‑1 (MQ=255)
                                                                       atTTACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTc                        >  1:2285523/1‑100 (MQ=255)
                                                                        tttACGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCa                       <  1:1131886/100‑1 (MQ=255)
                                                                           aCGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGc                     <  1:2456630/99‑1 (MQ=255)
                                                                           aCGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCa                    <  2:751124/100‑1 (MQ=255)
                                                                            cGGCGCAGCCAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCAc                   <  1:1094927/100‑1 (MQ=255)
                                                                                    ccAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcgt           >  1:1193624/1‑100 (MQ=255)
                                                                                     naaGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcgtc          >  2:1829064/2‑100 (MQ=255)
                                                                                     cAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcgtc          >  1:2387620/1‑100 (MQ=255)
                                                                                     cAAGATTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcgtc          >  1:1951/1‑100 (MQ=255)
                                                                                         aTTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCAGCCACGCTTTCAGCACTTCTACGTCGTg       <  2:1378936/99‑1 (MQ=255)
                                                                                         aTTTCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGa      <  2:2100985/100‑1 (MQ=255)
                                                                                          tttCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGAc     <  2:1189357/100‑1 (MQ=255)
                                                                                            tCCCAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGc   >  1:2102111/1‑100 (MQ=255)
                                                                                             cccAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGcc  >  2:1591445/1‑100 (MQ=255)
                                                                                             cccAGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGcc  <  2:1690904/100‑1 (MQ=255)
                                                                                             cccAGGTGTTGGCGCAGTATTCGCGCACCCCGGACTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCCCGCTTTCAGCACTTCTACGTCGTGACGcc  >  2:1540379/1‑100 (MQ=255)
                                                                                                |                                                                                                 
ATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCC  >  NC_000913/3350552‑3350744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: