Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,443,086 T→A 100% N338Y (AAC→TAC)  secY ← preprotein translocase membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,0860TA98.0% 338.8 / ‑1.9 101N338Y (AAC→TAC) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (49/50);  total (49/52)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

Only 100 of 101 total aligned reads displayed.

ATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAA  >  NC_000913/3442989‑3443180
                                                                                                 |                                                                                              
aTTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGa                                                                                              >  2:2175291/1‑100 (MQ=255)
  tACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGaaa                                                                                            <  1:888555/100‑1 (MQ=255)
     tttATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAAcc                                                                                         <  1:177136/100‑1 (MQ=255)
      ttATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCa                                                                                        <  2:1295057/100‑1 (MQ=255)
      ttATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCa                                                                                        >  2:1997669/1‑100 (MQ=255)
       tATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCaa                                                                                       <  1:956366/100‑1 (MQ=255)
         tCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACg                                                                                     >  1:779616/1‑100 (MQ=255)
         tCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACg                                                                                     <  2:2120105/100‑1 (MQ=255)
          cggtataCTTCGCCGTTGGCCCTCTCGTTCGAATTCCTGGTACAAATGCACCGGAGTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAAc                                                                                          <  2:842496/91‑1 (MQ=255)
          cGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGc                                                                                    >  2:1087662/1‑100 (MQ=255)
          cGATATACTTCGCCGTTTGCTCTCACGGACAAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAAAGc                                                                                    >  2:1608590/1‑100 (MQ=255)
           gATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGcc                                                                                   <  1:2350139/100‑1 (MQ=255)
           gATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCGGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGcc                                                                                   <  1:520763/100‑1 (MQ=255)
             tataCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAGTGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCgt                                                                                 <  2:1803722/100‑1 (MQ=255)
             tataCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCgt                                                                                 >  1:402242/1‑100 (MQ=255)
               taCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCgtgt                                                                               >  1:427414/1‑100 (MQ=255)
               taCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCgtgt                                                                               <  2:2229339/100‑1 (MQ=255)
               taCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCgtgt                                                                               <  1:2145529/100‑1 (MQ=255)
                aCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTa                                                                              <  2:202198/100‑1 (MQ=255)
                aCTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTa                                                                              >  1:2057173/1‑100 (MQ=255)
                 cTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTag                                                                             <  1:806522/100‑1 (MQ=255)
                  ttCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTaga                                                                            >  2:1188422/1‑100 (MQ=255)
                   tCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaa                                                                           >  1:2025823/1‑100 (MQ=255)
                    cGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaag                                                                          <  1:1853473/100‑1 (MQ=255)
                    cGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaag                                                                          <  2:1319770/100‑1 (MQ=255)
                    cGCCGTTTGCTCTCCCCGACGAATTCCTGGCAGAAATGCACCTGACTTCTGCAGGTTATCTGCTGTGTCACGCGGGTAGAAAACCAACGCCGTGTagaag                                                                          <  2:1257868/100‑1 (MQ=255)
                     gCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaaga                                                                         <  1:2077687/100‑1 (MQ=255)
                     gCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaaga                                                                         <  1:1308142/100‑1 (MQ=255)
                     gCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaaga                                                                         <  2:798599/100‑1 (MQ=255)
                       cGTCTGCTCGCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTGATCTGGTGTTTCACGCGGGTAGAAAACCAACGCCGTGTagaa                                                                           <  2:289277/96‑1 (MQ=255)
                        gTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAAc                                                                      <  1:1505652/100‑1 (MQ=255)
                         tttGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACa                                                                     <  1:338081/100‑1 (MQ=255)
                                 cccGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaagaaga                                                             <  2:322233/100‑1 (MQ=255)
                                 cccGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACagaagaaga                                                             >  1:1174737/1‑100 (MQ=255)
                                  ccGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAgat                                                            <  1:218180/100‑1 (MQ=255)
                                    ggACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAgatga                                                          >  1:1579897/1‑100 (MQ=255)
                                    ggACGAATTCCTGGTACAAACGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAgatga                                                          >  2:1562426/1‑100 (MQ=255)
                                     gACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAGACCAACGCCGTGTAGAAGAAACAGAAGAAgatgat                                                         >  2:596574/1‑100 (MQ=255)
                                     gACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAgatgat                                                         <  1:1818191/100‑1 (MQ=255)
                                       cGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTg                                                       <  1:2194103/100‑1 (MQ=255)
                                       cGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAAAGCCGTGTAGAAGAAACAGAAGAAgatgat                                                         >  2:2130683/1‑98 (MQ=255)
                                       cGAATTCCTGGTACAAATGCACCGGACATCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTg                                                       >  2:791722/1‑100 (MQ=255)
                                         aaTTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCa                                                     <  2:308975/100‑1 (MQ=255)
                                          aTTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAg                                                    <  1:2500509/100‑1 (MQ=255)
                                          aTTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAg                                                    <  1:788320/100‑1 (MQ=255)
                                             ccTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACg                                                 >  1:221668/1‑100 (MQ=255)
                                              cTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGc                                                >  1:1240347/1‑100 (MQ=255)
                                              cTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGc                                                >  1:2140747/1‑100 (MQ=255)
                                                ggTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCa                                               <  1:940105/99‑1 (MQ=255)
                                                  tACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATag                                            <  2:1360590/100‑1 (MQ=255)
                                                  tACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATag                                            >  1:1789258/1‑100 (MQ=255)
                                                    cAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATagag                                          <  1:2417233/100‑1 (MQ=255)
                                                      aaTGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTa                                        >  1:731450/1‑100 (MQ=255)
                                                      aaTGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTa                                        >  1:1838628/1‑100 (MQ=255)
                                                      aaTGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTa                                        >  1:979399/1‑100 (MQ=255)
                                                      aaTGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTa                                        <  1:1452388/100‑1 (MQ=255)
                                                       aTGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTaa                                       <  2:1520574/100‑1 (MQ=255)
                                                          cACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAacac                                    >  1:2362669/1‑100 (MQ=255)
                                                          cACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAacac                                    >  1:15445/1‑100 (MQ=255)
                                                            ccGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACAt                                  >  1:1489105/1‑100 (MQ=255)
                                                            ccGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACAt                                  >  1:430255/1‑100 (MQ=255)
                                                              ggACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATaa                                >  2:1139112/1‑100 (MQ=255)
                                                                aCTTCTTCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAg                              <  1:632512/100‑1 (MQ=255)
                                                                 cttcttCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGc                             >  2:1722479/1‑100 (MQ=255)
                                                                 cttcttCAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGc                             >  2:2490757/1‑100 (MQ=255)
                                                                   tcttcAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCgg                           >  2:1818196/1‑100 (MQ=255)
                                                                   tcttcAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCgg                           >  1:506347/1‑100 (MQ=255)
                                                                   tattcAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCgg                           <  2:437718/98‑1 (MQ=255)
                                                                    cttcAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGt                          >  2:972498/1‑100 (MQ=255)
                                                                      tcAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTg                        >  1:1709046/1‑100 (MQ=255)
                                                                      tcAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTg                        <  1:2306881/100‑1 (MQ=255)
                                                                       cAGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGc                       <  1:620366/100‑1 (MQ=255)
                                                                        aGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGcc                      <  2:776650/100‑1 (MQ=255)
                                                                        aGGTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGcc                      <  1:966343/100‑1 (MQ=255)
                                                                         ggTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGccc                     >  2:2413583/1‑100 (MQ=255)
                                                                          gTTATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCa                    >  2:1547174/1‑100 (MQ=255)
                                                                           ttATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAg                   <  2:1695022/100‑1 (MQ=255)
                                                                           ttATCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAg                   <  2:1297906/100‑1 (MQ=255)
                                                                           tgatatgctgTTTCACGCGGGTAGAAGACCACCGCCGTGTAGTAGAAACAGAAGAAGAGGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAg                   <  2:1805696/95‑1 (MQ=255)
                                                                              tCTGCTGTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCt                <  1:1889589/100‑1 (MQ=255)
                                                                               ctgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTg               <  1:806865/100‑1 (MQ=255)
                                                                               ctgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTg               >  1:2341412/1‑100 (MQ=255)
                                                                                tgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGc              <  1:1051352/100‑1 (MQ=255)
                                                                                tgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGc              >  2:846971/1‑100 (MQ=255)
                                                                                tgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGc              >  2:118468/1‑100 (MQ=255)
                                                                                tgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAAGCTg               >  2:841624/1‑99 (MQ=255)
                                                                                tgctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAAGCTGc              >  2:775580/1‑100 (MQ=255)
                                                                                 gctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCa             >  1:1427732/1‑100 (MQ=255)
                                                                                 gctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCa             <  1:125824/100‑1 (MQ=255)
                                                                                  ctgTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTACaa            <  1:1774031/100‑1 (MQ=255)
                                                                                    gTTTCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAAt          >  1:1053051/1‑100 (MQ=255)
                                                                                     tttCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATa         >  2:1872117/1‑100 (MQ=255)
                                                                                      ttCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATAc        >  2:1097338/1‑100 (MQ=255)
                                                                                      ttCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATAc        >  2:1147041/1‑100 (MQ=255)
                                                                                      ttCACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATAc        >  2:825438/1‑100 (MQ=255)
                                                                                        cACGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTCCAGACGCATAGAGTAACACATAACGCGGTTGCCCAGGCTGCAAATACAg      >  2:1267496/1‑100 (MQ=255)
                                                                                         aCGCGGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGc     <  1:672555/100‑1 (MQ=255)
                                                                                          cgcgGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCg    <  1:2150449/100‑1 (MQ=255)
                                                                                            cgGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGaa  <  2:800036/100‑1 (MQ=255)
                                                                                            cgGGTAGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGaa  >  2:1457127/1‑100 (MQ=255)
                                                                                                 |                                                                                              
ATTACTTTATCGATATACTTCGCCGTTTGCTCTCCCGGACGAATTCCTGGTACAAATGCACCGGACTTCTTCAGGTTATCTGCTGTTTCACGCGGGTTGAAAACCAACGCCGTGTAGAAGAAACAGAAGAAGATGATTGCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAA  >  NC_000913/3442989‑3443180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: