Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,477,434 T→C 100% intergenic (‑15/‑206) fkpA ← / → slyX FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)/phi X174 lysis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,477,4340TC99.1% 384.8 / ‑4.7 111intergenic (‑15/‑206)fkpA/slyXFKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)/phi X174 lysis protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (43/67);  total (44/67)
Fisher's exact test for biased strand distribution p-value = 3.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01

Only 100 of 111 total aligned reads displayed.

CTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATT  >  NC_000913/3477338‑3477530
                                                                                                |                                                                                                
cTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCgg                                                                                               <  2:926837/100‑1 (MQ=255)
 ttCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCggg                                                                                              <  2:1324400/100‑1 (MQ=255)
  tCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCgggg                                                                                             <  2:893881/100‑1 (MQ=255)
  tCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCgggg                                                                                             <  1:1389008/100‑1 (MQ=255)
  tCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCgggg                                                                                             <  1:167690/100‑1 (MQ=255)
     gcagcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAt                                                                                          >  1:1638827/1‑100 (MQ=255)
     gcagcaAAAGTGATTGGTGCATGCAAGGCAACTTCCATTGTGGTCGCCACCAGCGTTACTTTAAACAGTGATTTCTTCCATATCTCCAGGACCgggg                                                                                             <  2:2548863/97‑1 (MQ=255)
      cagcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtc                                                                                         >  2:209346/1‑100 (MQ=255)
        gcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtctc                                                                                       >  1:94876/1‑100 (MQ=255)
        gcaAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCAtctc                                                                                       <  1:2006209/100‑1 (MQ=255)
          aaaaGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAc                                                                                     >  1:1113716/1‑100 (MQ=255)
          aaaaGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAc                                                                                     <  1:2280215/100‑1 (MQ=255)
           aaaGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAcc                                                                                    <  1:385437/100‑1 (MQ=255)
           aaaGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAcc                                                                                    >  2:284324/1‑100 (MQ=255)
             aGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAccct                                                                                  >  1:2182216/1‑99 (MQ=255)
             aGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCAcccc                                                                                  <  2:100687/100‑1 (MQ=255)
               tGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAg                                                                                >  1:1099503/1‑100 (MQ=255)
                 aTTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAggg                                                                              <  2:1464091/100‑1 (MQ=255)
                    ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTa                                                                           >  2:2176962/1‑100 (MQ=255)
                    ggTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTGTAAACAATGATTTCATACATATCTCCAGGCCCGGGGGATCTCACCCCAGGGTTa                                                                           >  2:2336225/1‑100 (MQ=255)
                      tgcatgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAAc                                                                         >  1:628008/1‑100 (MQ=255)
                         atgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtat                                                                      >  1:53957/1‑100 (MQ=255)
                         atgcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtat                                                                      >  1:230824/1‑100 (MQ=255)
                           gcaGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtatta                                                                    >  2:2441516/1‑100 (MQ=255)
                            caGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtattat                                                                   >  1:2104787/1‑100 (MQ=255)
                            caGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACtatt                                                                     >  2:1854461/1‑98 (MQ=255)
                             aGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCATGACCGGGGCATCTCACCCCAGGGTTAACtatta                                                                    <  2:1152336/98‑1 (MQ=255)
                             aGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATc                                                                  <  1:2265588/100‑1 (MQ=255)
                             aGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCACAGGGTTAACTATTATc                                                                  <  2:1141500/100‑1 (MQ=255)
                              gggatccGGTCATTGTGATCGCCAGCTGCGGTACTTTAAACCGTGACTTCATACATAACTCCAGGACCGGGGCAACTCACCTCAGGGTTAACtattataa                                                                 <  2:2148268/94‑3 (MQ=255)
                              gggCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCa                                                                 <  2:577562/100‑1 (MQ=255)
                               ggCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAg                                                                <  1:935011/100‑1 (MQ=255)
                                  aaCGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAg                                                             <  1:2347024/100‑1 (MQ=255)
                                      gCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCATAAGGGTa                                                         >  2:894773/1‑100 (MQ=255)
                                       ccATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTCTCAGAAGGGTAc                                                        <  1:1687826/100‑1 (MQ=255)
                                        cATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACACCAGGGTTAACTATTATCAGAAGGGTACt                                                       >  2:2441371/1‑100 (MQ=255)
                                         aTTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCATGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACta                                                      >  2:1014150/1‑100 (MQ=255)
                                          ttGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACtat                                                     <  1:1502426/100‑1 (MQ=255)
                                           tgtgGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACtata                                                    <  2:1638815/100‑1 (MQ=255)
                                              ggTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAg                                                 <  1:913634/100‑1 (MQ=255)
                                               gTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGc                                                <  1:1207069/100‑1 (MQ=255)
                                               gTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACCATAAAGc                                                <  1:1346843/100‑1 (MQ=255)
                                                 cGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGt                                              <  1:1613431/100‑1 (MQ=255)
                                                 cGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGt                                              <  2:2104773/100‑1 (MQ=255)
                                                    cagcagCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTg                                            >  1:2135126/1‑99 (MQ=255)
                                                     agcagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTc                                          >  1:77680/1‑100 (MQ=255)
                                                     agcagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTc                                          <  1:1693414/100‑1 (MQ=255)
                                                      gcagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCg                                         <  2:671922/100‑1 (MQ=255)
                                                      gcagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCg                                         <  2:1631210/100‑1 (MQ=255)
                                                       cagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGa                                        <  1:1252777/100‑1 (MQ=255)
                                                       cagcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGa                                        <  1:652730/100‑1 (MQ=255)
                                                         gcGTTTCTTTAAACAGTGATCTCATCCATATCTACAGGACCGGGGCATCTCACCCCACGGTTAACTATTACCAGAAGGGTACTATAAAGCGTTGCCGAAc                                      <  2:2517275/100‑1 (MQ=255)
                                                         gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCg                                         <  2:945817/97‑1 (MQ=255)
                                                         gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                      <  2:2367142/100‑1 (MQ=255)
                                                         gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                      <  1:1270578/100‑1 (MQ=255)
                                                         gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                      <  2:2367164/100‑1 (MQ=255)
                                                         gcGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAAc                                      <  1:603435/100‑1 (MQ=255)
                                                          cGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACa                                     <  1:2441530/100‑1 (MQ=255)
                                                               cTTTAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCt                                >  2:2533960/1‑100 (MQ=255)
                                                                tttAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCta                               <  2:112052/100‑1 (MQ=255)
                                                                tttAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCta                               <  2:631966/100‑1 (MQ=255)
                                                                 ttAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtac                              <  1:1992196/100‑1 (MQ=255)
                                                                 ttAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtac                              >  1:2361385/1‑100 (MQ=255)
                                                                 ttAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtac                              <  2:815211/100‑1 (MQ=255)
                                                                 ttAAACAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtac                              <  1:1704660/100‑1 (MQ=255)
                                                                   aaaCAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacat                            <  1:291884/100‑1 (MQ=255)
                                                                   aaaCAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacat                            <  2:367751/100‑1 (MQ=255)
                                                                    aaCAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacata                           <  1:286604/100‑1 (MQ=255)
                                                                    aaCAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacata                           <  2:1822619/100‑1 (MQ=255)
                                                                     aCAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGCGTTAACTATTATCAGCAGGGTACTATAAAGCGTTGTCGAACAAATCtacatac                          >  2:2274401/1‑100 (MQ=255)
                                                                      cAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCGGGGTTAACTATTCTCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacataca                         >  2:1489189/1‑100 (MQ=255)
                                                                      cAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacataca                         >  1:1979965/1‑100 (MQ=255)
                                                                      cAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCtacataca                         <  2:1471299/100‑1 (MQ=255)
                                                                      cAGTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTCGTCGAACAAATCtacataca                         <  2:1568801/100‑1 (MQ=255)
                                                                        gTGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGa                       <  1:2154063/100‑1 (MQ=255)
                                                                         tGATTTCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAACCGCGGTCGAACAAATCTACATACAGac                      <  2:559382/100‑1 (MQ=255)
                                                                            tttCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACg                   <  1:1669595/100‑1 (MQ=255)
                                                                              tCATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGcc                 >  1:1615832/1‑100 (MQ=255)
                                                                               cATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACg                   >  2:231728/1‑97 (MQ=255)
                                                                               cATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGccc                <  2:1804589/100‑1 (MQ=255)
                                                                               cATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGccc                >  2:1383031/1‑100 (MQ=255)
                                                                               cATCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGccc                >  1:824771/1‑100 (MQ=255)
                                                                                aTCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGccc                >  1:565422/1‑99 (MQ=255)
                                                                                aTCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCt               <  2:688611/100‑1 (MQ=255)
                                                                                aTCCATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCt               >  2:59499/1‑100 (MQ=255)
                                                                                  ccATAACTCCAGGACCGGGGCATCTCACCCCAGGGTTAACCATTATCAGAAGGGTACTATCCCCCGTTGTCGAACAACTCTACATACAGACACGccc                <  2:2511726/97‑1 (MQ=255)
                                                                                   cATATCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCAtatt            >  1:1180348/1‑100 (MQ=255)
                                                                                    atatCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtatta           >  1:466451/1‑100 (MQ=255)
                                                                                     tatCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtattat          >  2:19363/1‑100 (MQ=255)
                                                                                      atCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatc         <  2:61525/100‑1 (MQ=255)
                                                                                      atCTCCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatc         <  1:552371/100‑1 (MQ=255)
                                                                                      atCTCCAGGACCGGGGCATCGCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCtattac          >  2:1559533/1‑98 (MQ=255)
                                                                                       tctcCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTAAAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatca        <  2:1866106/100‑1 (MQ=255)
                                                                                        ctcCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcat       >  1:186152/1‑100 (MQ=255)
                                                                                        ctcCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatca        <  2:109505/99‑1 (MQ=255)
                                                                                         tcCAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTatcatc      <  1:2508053/100‑1 (MQ=255)
                                                                                          ccAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCt     <  1:1356704/100‑1 (MQ=255)
                                                                                           cAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTa    >  1:1003158/1‑100 (MQ=255)
                                                                                           cAGGACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTa    >  2:2560082/1‑100 (MQ=255)
                                                                                             ggACCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCAACATACAGACACGCCCTATTATCATCTAtt  >  2:456122/1‑100 (MQ=255)
                                                                                                |                                                                                                
CTTCAGCAGCAAAAGTGATTGGTGCATGCAGGGCAACGGCCATTGTGGTCGCCAGCAGCGTTACTTTAAACAGTGATTTCATCCATATCTCCAGGATCGGGGCATCTCACCCCAGGGTTAACTATTATCAGAAGGGTACTATAAAGCGTTGTCGAACAAATCTACATACAGACACGCCCTATTATCATCTATT  >  NC_000913/3477338‑3477530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: