Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,524,413 A→G 100% I515V (ATT→GTT)  mrcA → penicillin‑binding protein 1a, murein transglycosylase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,524,4130AG97.1% 330.4 / ‑0.8 102I515V (ATT→GTT) mrcApenicillin‑binding protein 1a, murein transglycosylase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (3/0);  new base G (55/44);  total (58/44)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 102 total aligned reads displayed.

GGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAAC  >  NC_000913/3524316‑3524509
                                                                                                 |                                                                                                
ggCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTCCTGACTGGCGGCCGAGGAACTCACGACCGCAGTATGCTGGTCCAGtt                                                                                                >  2:1727542/1‑100 (MQ=255)
   aaGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGc                                                                                             <  2:126855/100‑1 (MQ=255)
    aGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCt                                                                                            <  2:1318526/100‑1 (MQ=255)
    aGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCt                                                                                            <  1:2450374/100‑1 (MQ=255)
     gTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCtt                                                                                           >  1:479183/1‑100 (MQ=255)
        tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACg                                                                                        >  1:362989/1‑100 (MQ=255)
        tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACg                                                                                        >  1:1650467/1‑100 (MQ=255)
        tGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACg                                                                                        >  1:339768/1‑100 (MQ=255)
         gTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGt                                                                                       >  2:167947/1‑100 (MQ=255)
         gTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGt                                                                                       >  1:2155413/1‑100 (MQ=255)
          ttGAACGGTGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACTACCGCAGTATGCTGGTCCAGTTCGCTTACGTc                                                                                      >  2:2198314/1‑100 (MQ=255)
          ttGAACGATGTGCCAATTTCTCGCTGGGCTGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCATTATGCTGGTCCAGTTCGCTTACGTc                                                                                      >  2:1671452/1‑100 (MQ=255)
           tGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCa                                                                                     >  1:438134/1‑100 (MQ=255)
           tGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCa                                                                                     >  1:751899/1‑100 (MQ=255)
           tGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCa                                                                                     <  1:2348804/100‑1 (MQ=255)
           tGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCa                                                                                     >  2:84368/1‑100 (MQ=255)
            gAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAg                                                                                    >  1:2190930/1‑100 (MQ=255)
            gAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAg                                                                                    >  2:1840009/1‑100 (MQ=255)
             aaCGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAgg                                                                                   >  2:2096368/1‑100 (MQ=255)
              aCGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAggg                                                                                  <  1:1974849/100‑1 (MQ=255)
              aCGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAggg                                                                                  <  1:134980/100‑1 (MQ=255)
               cGATGTGCCAATTTCTCGCTGTGATGCAAGCGCCGGCTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAggg                                                                                  <  2:1727570/99‑1 (MQ=255)
                  tgtgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTg                                                                              <  1:1017665/100‑1 (MQ=255)
                   gtgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTgg                                                                             >  2:897468/1‑100 (MQ=255)
                    tgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGc                                                                                >  2:401175/1‑96 (MQ=255)
                    tgCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGCTCGCTTACGCCCGGGGCTgg                                                                             >  2:108928/1‑99 (MQ=255)
                    atctaaTTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTggg                                                                            <  2:2049764/96‑1 (MQ=255)
                     gCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGt                                                                           >  2:1268508/1‑100 (MQ=255)
                     gCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGt                                                                           <  1:144438/100‑1 (MQ=255)
                      ccAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTc                                                                          <  2:154314/100‑1 (MQ=255)
                      ccAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTc                                                                          <  1:360790/100‑1 (MQ=255)
                      ccAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTc                                                                          >  1:1961424/1‑100 (MQ=255)
                      ccAATTTCGCGCTGGGATGCAAGTGCCGGTTTTGACTGGCGGCCGAAGAACTCACCACTGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTc                                                                          <  2:1397421/100‑1 (MQ=255)
                       cAATTTCTCTCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTc                                                                          <  2:1239461/99‑1 (MQ=255)
                       cAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCa                                                                         >  1:2234647/1‑100 (MQ=255)
                        aaTTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTgggagat                                                                        >  1:2049778/1‑96 (MQ=255)
                             ctcGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGaa                                                                   >  1:969817/1‑100 (MQ=255)
                              tcGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGaaa                                                                  <  2:54947/100‑1 (MQ=255)
                              tcGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGaaa                                                                  <  1:2554226/100‑1 (MQ=255)
                              tcGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGaaa                                                                  <  2:2146692/100‑1 (MQ=255)
                               cGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGaaaa                                                                 <  2:324323/100‑1 (MQ=255)
                               cGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGaaaa                                                                 <  2:333773/100‑1 (MQ=255)
                                   gggATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgt                                                             <  1:801167/100‑1 (MQ=255)
                                   gggATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgt                                                             >  2:2512088/1‑100 (MQ=255)
                                   gggATGCAAGTGCCGGTTCTGACTGGAAGCGGAAGAACTCACTAGAGGAGTATGCTGGTCCAGTTGGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgt                                                             >  2:2130969/1‑100 (MQ=255)
                                    ggATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtg                                                            >  2:1999741/1‑100 (MQ=255)
                                     gATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtgg                                                           >  1:2288425/1‑100 (MQ=255)
                                      aTGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtggt                                                          <  1:696881/100‑1 (MQ=255)
                                       tGCAAGTGCGGGTGCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGATAAGAAAGAAAAAAAACgtggtg                                                         <  2:769521/100‑1 (MQ=255)
                                       tGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtggtg                                                         <  1:2308043/100‑1 (MQ=255)
                                        gCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGa                                                        >  1:211625/1‑100 (MQ=255)
                                         cAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtggtg                                                         >  1:2063740/1‑98 (MQ=255)
                                          aaGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATg                                                      <  1:2000988/100‑1 (MQ=255)
                                          aaGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATg                                                      <  1:1998270/100‑1 (MQ=255)
                                          aaGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGGCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACgtgg                                                           <  2:1201562/95‑1 (MQ=255)
                                            gTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGt                                                    >  1:579517/1‑100 (MQ=255)
                                             tGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTa                                                   <  1:2533172/100‑1 (MQ=255)
                                              gCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGt                                                    <  2:1732134/98‑1 (MQ=255)
                                                  ggtCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGATCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACgcgc                                              >  1:1734386/3‑100 (MQ=255)
                                                  gTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACgcgc                                              >  2:906046/1‑100 (MQ=255)
                                                    tCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCaa                                            >  1:1606592/1‑100 (MQ=255)
                                                      tGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATg                                          >  2:468177/1‑100 (MQ=255)
                                                      tGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATg                                          >  2:351727/1‑100 (MQ=255)
                                                       gACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGc                                         >  2:723025/1‑100 (MQ=255)
                                                       gACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGc                                         >  1:580825/1‑100 (MQ=255)
                                                       gACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGc                                         >  2:2104732/1‑100 (MQ=255)
                                                        aCTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCg                                        <  2:2234633/100‑1 (MQ=255)
                                                         cTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCgg                                       <  1:474983/100‑1 (MQ=255)
                                                         cTGGCAGCCGAAGAACTCACCACAGTAGCATGCTGGTCCAGTTCGCTTCCCTCAGGGGCCGGGTCAGTCGAAAAACGTGGTGATGGTCCgcgc                                              >  2:1618942/1‑93 (MQ=255)
                                                           ggCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGc                                     <  1:1819452/100‑1 (MQ=255)
                                                            gCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCg                                    <  1:1176817/100‑1 (MQ=255)
                                                             cAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCg                                    >  1:2329089/1‑99 (MQ=255)
                                                             cAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGa                                   >  1:861111/1‑100 (MQ=255)
                                                                 cGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATggg                               >  2:1023788/1‑100 (MQ=255)
                                                                  gaagaaCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGc                              >  2:751864/1‑100 (MQ=255)
                                                                     gaaCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTc                           >  1:784836/1‑100 (MQ=255)
                                                                      aaCTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGTAATGCGGGCGATGGGCGTCg                          <  2:1630021/100‑1 (MQ=255)
                                                                        cTCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGAc                        >  1:35405/1‑100 (MQ=255)
                                                                         tCACCACCGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGCCATGGTACGCCCAACGCGGGCgc                                   >  2:2341858/1‑87 (MQ=255)
                                                                           accaccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTAc                     <  2:235919/100‑1 (MQ=255)
                                                                            ccaccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTAca                    >  1:737802/1‑99 (MQ=255)
                                                                            ccaccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACg                    >  2:696523/1‑100 (MQ=255)
                                                                             caccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGc                   <  1:1465308/100‑1 (MQ=255)
                                                                             caccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCGGGGTCAGGCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGGCGACTACGc                   >  2:1014154/1‑100 (MQ=255)
                                                                              accGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCt                  <  1:1410876/100‑1 (MQ=255)
                                                                               ccGCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTg                 >  2:672707/1‑100 (MQ=255)
                                                                                 gCAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCa               <  1:751871/100‑1 (MQ=255)
                                                                                  cAGTATGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAg              <  1:2337723/100‑1 (MQ=255)
                                                                                  cAGTATGCTGGTCCAGTTCGCTTACGTCAGCGGCTGCGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGTCGATGGGCGTCGACGACGCTGc                <  2:850440/98‑1 (MQ=255)
                                                                                      aTGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAata          <  2:1919106/100‑1 (MQ=255)
                                                                                      aTGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAata          >  2:179266/1‑100 (MQ=255)
                                                                                       tGCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAatat         >  2:624592/1‑100 (MQ=255)
                                                                                        gCTGGTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATc        >  2:150070/1‑100 (MQ=255)
                                                                                           ggTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGc     <  1:2441744/100‑1 (MQ=255)
                                                                                            gTCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGCCTACGCTGCAGAATATCTGCa    >  2:1945162/1‑100 (MQ=255)
                                                                                             tCCAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGcga   >  2:609782/1‑98 (MQ=255)
                                                                                              ccAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAAc  >  2:1967161/1‑100 (MQ=255)
                                                                                              ccAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAAc  <  1:624664/100‑1 (MQ=255)
                                                                                              ccAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAAc  <  1:334621/100‑1 (MQ=255)
                                                                                              ccAGTTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCCTCGGCTACGCTGCAGAATATCTGCAAc  >  2:2293492/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GGCAAGTATGTTGAACGATGTGCCAATTTCTCGCTGGGATGCAAGTGCCGGTTCTGACTGGCAGCCGAAGAACTCACCACCGCAGTATGCTGGTCCAATTCGCTTACGTCAGGGGCTGGGTCAGTCGAAAAACGTGGTGATGGTACGCGCAATGCGGGCGATGGGCGTCGACTACGCTGCAGAATATCTGCAAC  >  NC_000913/3524316‑3524509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: