Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,648,042 T→C 100% intergenic (‑254/‑486) dinQ ← / → arsR UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system/arsenical resistance operon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,648,0420TC97.9% 325.1 / ‑2.5 95intergenic (‑254/‑486)dinQ/arsRUV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system/arsenical resistance operon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (51/42);  total (53/42)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01

TATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGG  >  NC_000913/3647945‑3648137
                                                                                                 |                                                                                               
tATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCTGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCAt                                                                                               >  2:1802133/1‑100 (MQ=255)
   gcgcATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGt                                                                                            <  2:16713/100‑1 (MQ=255)
      cATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTAtt                                                                                         <  1:864770/100‑1 (MQ=255)
       aTTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATTATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTa                                                                                        <  1:1800751/100‑1 (MQ=255)
       aTTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTa                                                                                        <  1:1119076/100‑1 (MQ=255)
       aTTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTa                                                                                        <  1:1435527/100‑1 (MQ=255)
          gCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCa                                                                                     >  2:1742011/1‑100 (MQ=255)
          gCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCa                                                                                     >  1:1218150/1‑100 (MQ=255)
           ccAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCAt                                                                                    <  2:1416362/100‑1 (MQ=255)
           ccAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCAt                                                                                    >  2:2466236/1‑100 (MQ=255)
           ccAGAGGGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCa                                                                                     <  2:2471626/99‑1 (MQ=255)
              gagTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGa                                                                                 >  1:398929/1‑100 (MQ=255)
              gagTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGa                                                                                 >  2:2533247/1‑100 (MQ=255)
              gagTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGGTTCAGCATGGTAGTAGCAGTg                                                                                  >  1:1759329/1‑99 (MQ=255)
              gagTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGCTTCAGCATGGTATTAGCATTGa                                                                                 >  2:684032/1‑100 (MQ=255)
               agTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGAt                                                                                >  1:545656/1‑100 (MQ=255)
                gTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATg                                                                               <  1:1055542/100‑1 (MQ=255)
                  gAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCg                                                                             <  1:39843/100‑1 (MQ=255)
                  gAAAATGCCAAAGAGCTAACTGGATAATCATACGGGACATGTAGGTTATAAAATCAGAGCGTCCTTGCAATAGTTTCAGCATGTTATTAGCATTGATg                                                                               <  2:2289685/98‑1 (MQ=255)
                     aaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTa                                                                          >  1:2540909/1‑100 (MQ=255)
                     aaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTa                                                                          <  1:1979304/100‑1 (MQ=255)
                     aaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTa                                                                          <  1:1062770/100‑1 (MQ=255)
                     aaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTa                                                                          <  1:1188653/100‑1 (MQ=255)
                     aaTGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTa                                                                          <  2:2523691/100‑1 (MQ=255)
                        gCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAgat                                                                       <  2:536954/100‑1 (MQ=255)
                            cAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGatgatg                                                                   >  2:2006811/1‑100 (MQ=255)
                             agagCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATgg                                                                  <  1:175841/100‑1 (MQ=255)
                                ncTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTa                                                               >  1:1242280/2‑100 (MQ=255)
                                gCTAACTGGATAATCATACAGTACATGCAGGTTATAAGACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTa                                                               <  2:625833/100‑1 (MQ=255)
                                gCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCCGCACGTCCTTACAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTa                                                               >  1:1103781/1‑100 (MQ=255)
                                gCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTa                                                               >  2:191797/1‑100 (MQ=255)
                                gCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTa                                                               <  2:1447698/100‑1 (MQ=255)
                                gCTAACTGCTTATGCATTCAGTACATGCAGGTTATAACACCATCACGTCCTTGTAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTa                                                               <  2:821677/100‑1 (MQ=255)
                                  tAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTAtc                                                             >  1:2146464/1‑100 (MQ=255)
                                  tAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTAtc                                                             >  2:507548/1‑100 (MQ=255)
                                  tAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTAtc                                                             >  1:249809/1‑100 (MQ=255)
                                   aaCTGGATAATCCTACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTAtct                                                            <  2:677556/100‑1 (MQ=255)
                                   aaCTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTAtct                                                            >  2:13818/1‑100 (MQ=255)
                                    aCTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTAtctc                                                           <  1:1027325/100‑1 (MQ=255)
                                      tGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCAc                                                         >  2:775355/1‑100 (MQ=255)
                                      tGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCAc                                                         <  2:1065592/100‑1 (MQ=255)
                                      tGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCAc                                                         <  1:775362/100‑1 (MQ=255)
                                       ggATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACt                                                        >  2:1906854/1‑100 (MQ=255)
                                         aTAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTcc                                                      <  1:2533262/100‑1 (MQ=255)
                                         aTAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTcc                                                      <  1:1630671/100‑1 (MQ=255)
                                         aTAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGCTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTcc                                                      <  1:684039/100‑1 (MQ=255)
                                          tAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCa                                                     >  1:2089898/1‑100 (MQ=255)
                                           aaTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAg                                                    <  2:1128706/100‑1 (MQ=255)
                                           aaTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAg                                                    <  1:941890/100‑1 (MQ=255)
                                            aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGt                                                   >  1:2023868/1‑100 (MQ=255)
                                            aTCATACAGTACATGCAGGTTATAAAACCAGCACGACCTTGCAATAGATTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGt                                                   >  2:1455847/1‑100 (MQ=255)
                                              cATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCa                                                 <  1:1270856/100‑1 (MQ=255)
                                               aTACAGTACATGCAGGTTATAAACCCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCag                                                <  1:509445/100‑1 (MQ=255)
                                               aTACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCag                                                >  2:2532315/1‑100 (MQ=255)
                                               aTACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCag                                                >  2:1519885/1‑100 (MQ=255)
                                                tACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCaga                                               >  1:437879/1‑100 (MQ=255)
                                                  cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGc                                             >  1:1182300/1‑100 (MQ=255)
                                                   aGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGcc                                            <  1:1363275/100‑1 (MQ=255)
                                                      aCATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGccacc                                         >  1:821600/1‑100 (MQ=255)
                                                       cATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGccacca                                        >  1:2101443/1‑100 (MQ=255)
                                                       cATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGccacca                                        >  1:2204741/1‑100 (MQ=255)
                                                       cATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGccacca                                        >  1:998922/1‑100 (MQ=255)
                                                         tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAAc                                      >  1:10114/1‑100 (MQ=255)
                                                         tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAAc                                      <  1:2398402/100‑1 (MQ=255)
                                                         tGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAAc                                      >  1:2329069/1‑100 (MQ=255)
                                                          gCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACt                                     <  2:1906758/100‑1 (MQ=255)
                                                            aGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCa                                   >  1:1184760/1‑100 (MQ=255)
                                                              gTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAgg                                 >  1:1984409/1‑100 (MQ=255)
                                                              gTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAgg                                 <  1:2359966/100‑1 (MQ=255)
                                                                tataAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGc                               >  1:1491037/1‑100 (MQ=255)
                                                                tataAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGc                               >  1:2000923/1‑100 (MQ=255)
                                                                tataAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGc                               <  1:325274/100‑1 (MQ=255)
                                                                  taAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTg                             >  1:972019/1‑100 (MQ=255)
                                                                  taAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTg                             >  2:1208356/1‑100 (MQ=255)
                                                                  taAAACCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATATCACTCCAGTCAGAGCCACCAACTCAGGGCTg                             >  1:1550129/1‑100 (MQ=255)
                                                                    aaaCCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGa                           <  1:1391440/100‑1 (MQ=255)
                                                                      aCCAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGaaa                         >  2:918272/1‑100 (MQ=255)
                                                                       ccAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAg                        <  1:162753/100‑1 (MQ=255)
                                                                       ccAGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAg                        <  1:1742023/100‑1 (MQ=255)
                                                                         aGCACGTCCTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTa                      <  2:545649/100‑1 (MQ=255)
                                                                                ccTTGCACTAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAAcc               >  1:1344809/1‑100 (MQ=255)
                                                                                ccTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAAcc               >  1:1213714/1‑100 (MQ=255)
                                                                                 cTTGGAAGAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCAATCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCg              <  2:1335629/100‑1 (MQ=37)
                                                                                 cTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCg              >  2:622871/1‑100 (MQ=255)
                                                                                 cTTGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCg              >  2:626227/1‑100 (MQ=255)
                                                                                   tGCAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGAc            <  1:2315081/100‑1 (MQ=255)
                                                                                     cAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGc          >  1:380777/1‑100 (MQ=255)
                                                                                     cAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGc          >  2:1827962/1‑100 (MQ=255)
                                                                                     cAATAGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGc          >  1:1455737/1‑100 (MQ=255)
                                                                                         aGTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCa         >  2:1003255/1‑97 (MQ=255)
                                                                                          gTTTCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGt     >  1:1694863/1‑100 (MQ=255)
                                                                                           tttCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTc    >  1:1053060/1‑100 (MQ=255)
                                                                                            ttCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCg   >  2:159345/1‑100 (MQ=255)
                                                                                             tCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCgg  <  1:2197384/100‑1 (MQ=255)
                                                                                             tCAGCATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGACACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCgg  >  2:1894979/1‑100 (MQ=255)
                                                                                                 |                                                                                               
TATGCGCATTGCCAGAGTGAAAATGCCTCAGAGCTAACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAACCGACGCAAAGTCGG  >  NC_000913/3647945‑3648137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: