Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,657,599 T→A 16.1% intergenic (+32/‑767) hdeD → / → gadE acid‑resistance membrane protein/gad regulon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,657,5990TA16.1% 231.9 / 28.8 98intergenic (+32/‑767)hdeD/gadEacid‑resistance membrane protein/gad regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (41/40);  new base A (5/11);  total (47/51)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

GAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTT  >  NC_000913/3657500‑3657696
                                                                                                   |                                                                                                 
gAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACt                                                                                                   >  1:2066644/1‑100 (MQ=255)
gAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACt                                                                                                   >  1:1472810/1‑100 (MQ=255)
 aaTTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACtt                                                                                                  <  2:1328150/100‑1 (MQ=255)
  aTTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTg                                                                                                 <  1:1225603/100‑1 (MQ=255)
   ttgtccTGATATTTAGCGCCGCGAGCCTGTTCAGCTGCGCCAGTTTGTGCATTAAGCAGCAATAATGACCCCGGTTGTCACCCGGATCATAGTCACTTg                                                                                                 <  2:130795/94‑1 (MQ=255)
   ttGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGa                                                                                                <  2:1336699/100‑1 (MQ=255)
    tGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAt                                                                                               <  2:155855/100‑1 (MQ=255)
     gAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtg                                                                                              <  2:566878/100‑1 (MQ=255)
     gAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtg                                                                                              <  2:1759980/100‑1 (MQ=255)
     gAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAta                                                                                              >  1:155857/1‑99 (MQ=255)
      aaCTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtg                                                                                              <  2:1118194/99‑1 (MQ=255)
       aCTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtgtg                                                                                            <  2:1482743/100‑1 (MQ=255)
         tGATATTTAGGGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGAc                                                                                          <  2:2047722/100‑1 (MQ=255)
         tGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGAc                                                                                          >  1:1563931/1‑100 (MQ=255)
         tGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGAc                                                                                          >  1:1800333/1‑100 (MQ=255)
          gATAATTAGCGCCACCAGCCGGTTCAGCTTCGACAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACGCGGTGCATAGGCACGAGATGTGACt                                                                                         >  2:646383/1‑100 (MQ=255)
            tatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTAt                                                                                       <  1:2188321/100‑1 (MQ=255)
            tatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTAt                                                                                       <  2:245511/100‑1 (MQ=255)
            tatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTAt                                                                                       <  1:1426167/100‑1 (MQ=39)
            tatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTAt                                                                                       <  2:529012/100‑1 (MQ=39)
             atTTAGCTTCGCCAGCCTATTTAGCTTCGCTTGTTTTTTTGTTAAGCAGCAATAATTATCCCGGTTGTTGCCCGGATCATAGTCACATCAAGTGATTATg                                                                                      <  2:298734/100‑1 (MQ=255)
             atTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATg                                                                                      <  1:639450/100‑1 (MQ=39)
             atTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATg                                                                                      <  1:553187/100‑1 (MQ=39)
              tttAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGa                                                                                     <  1:437228/100‑1 (MQ=38)
               ttAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGAt                                                                                    <  2:651387/100‑1 (MQ=255)
               ttAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGAt                                                                                    <  1:687596/100‑1 (MQ=255)
                 aGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATcc                                                                                  <  1:1946645/100‑1 (MQ=255)
                 aGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGATcc                                                                                  <  2:2371991/100‑1 (MQ=255)
                  gcgcCGCCTGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGATcc                                                                                  <  1:1251778/99‑1 (MQ=38)
                  gcgcCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCg                                                                                 >  2:606432/1‑100 (MQ=255)
                  gcgcCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGATCCg                                                                                 <  1:1240109/100‑1 (MQ=38)
                   cgccgcCAGCCTGTTCCGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAACATAGTCACTTGATGTGACTATGATCCGa                                                                                >  2:698665/1‑100 (MQ=255)
                   cgccgcCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAGCATAGTCACTTGATGTGACTATGATCCg                                                                                 >  1:2160794/1‑99 (MQ=255)
                    gccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCg                                                                                 >  2:753183/1‑98 (MQ=255)
                     ccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGAtt                                                                              <  1:1123195/100‑1 (MQ=255)
                     ccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGAtt                                                                              <  1:1123181/100‑1 (MQ=255)
                       gccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGCTTa                                                                             >  2:467068/1‑99 (MQ=255)
                          aGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATAc                                                                         <  2:572262/100‑1 (MQ=255)
                          aGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAGCATAGTCACTTGATGTGACTATGATCCGATTAATAc                                                                         >  1:1032495/1‑100 (MQ=255)
                           gCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAACATAGTCACTTGATGTGACTATGATCCGATTAATAct                                                                        >  1:338344/1‑100 (MQ=255)
                            ccTGTTCAGCTTCTCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActc                                                                       <  1:1490962/100‑1 (MQ=255)
                            ccTGTTCAGCTTCACCCGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTCGTCACCCGGACCATAGTCACTTGATGTGACTATGATCCGATTAATAc                                                                         >  2:1148935/1‑98 (MQ=255)
                              tGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActctc                                                                     <  2:1645373/100‑1 (MQ=255)
                                 tCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTcc                                                                  <  2:714177/100‑1 (MQ=255)
                                    gCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCt                                                               >  1:297717/1‑100 (MQ=255)
                                    gCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCt                                                               >  1:298986/1‑100 (MQ=255)
                                    gCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCt                                                               >  2:114351/1‑100 (MQ=255)
                                       tCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTAcn                                                            <  2:830005/100‑2 (MQ=255)
                                       tCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACg                                                            <  2:338340/100‑1 (MQ=255)
                                         gCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGCTACGATGAATACTCTCTCCGCTACGc                                                           >  2:598617/1‑99 (MQ=255)
                                         gCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCa                                                          >  1:353430/1‑100 (MQ=255)
                                         gCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCa                                                          >  1:1580631/1‑100 (MQ=255)
                                         gCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCAGCTACGCa                                                          >  2:576666/1‑100 (MQ=255)
                                         gCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAACATAGTGAATTGATGTGACTATGATCCGATTAATACTCTCTCTGCTACg                                                            >  2:1030630/1‑98 (MQ=255)
                                          ccAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAg                                                         >  1:2495822/1‑100 (MQ=255)
                                          ccAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAg                                                         >  1:878101/1‑100 (MQ=255)
                                             gTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAgtgt                                                      <  1:1167753/100‑1 (MQ=255)
                                              tttGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgtt                                                     <  2:316672/100‑1 (MQ=255)
                                                tGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgttgt                                                   <  1:1112770/100‑1 (MQ=255)
                                                 gTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTa                                                  <  1:395121/100‑1 (MQ=255)
                                                 gTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTa                                                  <  2:468981/100‑1 (MQ=255)
                                                  ttCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAg                                                 >  2:2149483/1‑100 (MQ=255)
                                                  ttCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAg                                                 >  2:1901299/1‑100 (MQ=255)
                                                   tCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGa                                                >  2:1019429/1‑100 (MQ=255)
                                                    cGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGAt                                               >  1:2242351/1‑100 (MQ=255)
                                                    cGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAGCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGAt                                               >  2:1542298/1‑100 (MQ=255)
                                                    cGTTAAGCAGCAATAATTACCCCGGTTGTCACACGGATAATAGTCACTTGATGTGACTATGATCCGATTAATACTATCTCCGCTACGAAGTGTTGTAGAt                                               >  2:1542269/1‑100 (MQ=255)
                                                     gTTAAGCAGCAATGATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGAGCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATc                                              <  2:68441/100‑1 (MQ=255)
                                                     gTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATc                                              >  1:2048126/1‑100 (MQ=255)
                                                     gTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATc                                              >  1:377648/1‑100 (MQ=255)
                                                     gTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATc                                              <  2:323252/100‑1 (MQ=255)
                                                     gTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGAGCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATc                                              >  2:1010493/1‑100 (MQ=255)
                                                         agcagcAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAAtt                                          >  2:1696877/1‑100 (MQ=255)
                                                         agcagcAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAAtt                                          >  2:779610/1‑100 (MQ=255)
                                                         agcagcAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAAtt                                          >  2:328040/1‑100 (MQ=255)
                                                         accagcaATAATTACCCCGGTTGTCACCCGGGTCATAGTCACATGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAAtt                                          >  1:1734050/3‑100 (MQ=255)
                                                             gcaATACTTACCCCGGTTGTCACCCGGAACATAGTCACTTGATGAGACTATTATCCGACTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTgagc                                      >  2:444516/1‑100 (MQ=255)
                                                             gcaATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTTTGGTAGATCAGGTgcgc                                      >  1:2041626/1‑100 (MQ=255)
                                                              caATAATTACACCGGTTGTCACCCGGATCATAGTCACTTGATGTGAGTATGATCCGTTTAATACTCTCTCCGCTACGAAGTTTTGTAGAGCAATTGCGCa                                     >  2:2459578/1‑100 (MQ=255)
                                                                   atTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATc                                >  1:2384334/1‑100 (MQ=255)
                                                                   atTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATc                                >  1:520799/1‑100 (MQ=255)
                                                                       ccccGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCACACTATCATTg                            <  2:894502/100‑1 (MQ=255)
                                                                        cccGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGa                           <  1:2047820/100‑1 (MQ=255)
                                                                         gcGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTATGCAGTGTTGTAGATCAATTGCGCACTATCATTGaa                          <  2:364829/99‑1 (MQ=255)
                                                                          cGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGaaa                         <  1:101809/100‑1 (MQ=255)
                                                                                 cACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAc                  <  1:1435245/100‑1 (MQ=255)
                                                                                  aCCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAcc                 <  1:435183/100‑1 (MQ=255)
                                                                                  aCCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAcc                 <  1:767798/100‑1 (MQ=255)
                                                                                   cccGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCTGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCt                >  2:1810809/1‑100 (MQ=255)
                                                                                     cGGATCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGCAGATCAATTGCGCACTATCATTGAAATAATTACCTGc              >  1:495381/1‑100 (MQ=37)
                                                                                      ggATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCt             <  1:1667395/100‑1 (MQ=255)
                                                                                      ggAGCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATAGCGCACTATCATTGAAATAATTACCTGCt             >  1:1652673/1‑100 (MQ=38)
                                                                                       gATCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTa            >  1:681570/1‑100 (MQ=38)
                                                                                         tCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGt          <  2:2495807/100‑1 (MQ=255)
                                                                                          cATAGTCGCTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTg         >  1:1340392/1‑100 (MQ=255)
                                                                                           aTAGTCACATCAAGTGACTATCATCCGATTAATACTCTCTCCACTAAGCATTGCTGTAGATCAATTGCGCACCATCACTGAAATAATTAGCTGCTACTGa        >  2:627950/1‑100 (MQ=255)
                                                                                            tAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGat       <  2:1259238/100‑1 (MQ=255)
                                                                                               tctcTTGATGTGTCTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCTCACTATCATTGAAATAATTACCTGCTAGTGattat    <  2:2178269/97‑1 (MQ=255)
                                                                                               tCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGattat    <  2:1832140/100‑1 (MQ=255)
                                                                                                 aCTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTAttt  >  2:2426630/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
GAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTT  >  NC_000913/3657500‑3657696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: