Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,657,601 G→C 15.2% intergenic (+34/‑765) hdeD → / → gadE acid‑resistance membrane protein/gad regulon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,657,6010GC15.2% 258.0 / 36.4 98intergenic (+34/‑765)hdeD/gadEacid‑resistance membrane protein/gad regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base G (45/38);  new base C (4/11);  total (49/49)
Fisher's exact test for biased strand distribution p-value = 8.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

Only 100 of 101 total aligned reads displayed.

ATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAC  >  NC_000913/3657502‑3657700
                                                                                                   |                                                                                                   
aTTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTg                                                                                                     <  1:1225603/100‑1 (MQ=255)
 ttgtccTGATATTTAGCGCCGCGAGCCTGTTCAGCTGCGCCAGTTTGTGCATTAAGCAGCAATAATGACCCCGGTTGTCACCCGGATCATAGTCACTTg                                                                                                     <  2:130795/94‑1 (MQ=255)
 ttGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGa                                                                                                    <  2:1336699/100‑1 (MQ=255)
  tGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAt                                                                                                   <  2:155855/100‑1 (MQ=255)
   gAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtg                                                                                                  <  2:1759980/100‑1 (MQ=255)
   gAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtg                                                                                                  <  2:566878/100‑1 (MQ=255)
   gAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAta                                                                                                  >  1:155857/1‑99 (MQ=255)
    aaCTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtg                                                                                                  <  2:1118194/99‑1 (MQ=255)
     aCTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtgtg                                                                                                <  2:1482743/100‑1 (MQ=255)
       tGATATTTAGGGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGAc                                                                                              <  2:2047722/100‑1 (MQ=255)
       tGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGAc                                                                                              >  1:1800333/1‑100 (MQ=255)
       tGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGAc                                                                                              >  1:1563931/1‑100 (MQ=255)
        gATAATTAGCGCCACCAGCCGGTTCAGCTTCGACAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACGCGGTGCATAGGCACGAGATGTGACt                                                                                             >  2:646383/1‑100 (MQ=255)
          tatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTAt                                                                                           <  1:2188321/100‑1 (MQ=255)
          tatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTAt                                                                                           <  2:245511/100‑1 (MQ=255)
          tatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTAt                                                                                           <  1:1426167/100‑1 (MQ=39)
          tatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTAt                                                                                           <  2:529012/100‑1 (MQ=39)
           atTTAGCTTCGCCAGCCTATTTAGCTTCGCTTGTTTTTTTGTTAAGCAGCAATAATTATCCCGGTTGTTGCCCGGATCATAGTCACATCAAGTGATTATg                                                                                          <  2:298734/100‑1 (MQ=255)
           atTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATg                                                                                          <  1:553187/100‑1 (MQ=39)
           atTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATg                                                                                          <  1:639450/100‑1 (MQ=39)
            tttAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGa                                                                                         <  1:437228/100‑1 (MQ=38)
             ttAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGAt                                                                                        <  2:651387/100‑1 (MQ=255)
             ttAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGAt                                                                                        <  1:687596/100‑1 (MQ=255)
               aGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATcc                                                                                      <  1:1946645/100‑1 (MQ=255)
               aGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGATcc                                                                                      <  2:2371991/100‑1 (MQ=255)
                gcgcCGCCTGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGATcc                                                                                      <  1:1251778/99‑1 (MQ=38)
                gcgcCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCg                                                                                     >  2:606432/1‑100 (MQ=255)
                gcgcCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGATCCg                                                                                     <  1:1240109/100‑1 (MQ=38)
                 cgccgcCAGCCTGTTCCGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAACATAGTCACTTGATGTGACTATGATCCGa                                                                                    >  2:698665/1‑100 (MQ=255)
                 cgccgcCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAGCATAGTCACTTGATGTGACTATGATCCg                                                                                     >  1:2160794/1‑99 (MQ=255)
                  gccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCg                                                                                     >  2:753183/1‑98 (MQ=255)
                   ccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGAtt                                                                                  <  1:1123195/100‑1 (MQ=255)
                   ccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGAtt                                                                                  <  1:1123181/100‑1 (MQ=255)
                     gccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGCTTa                                                                                 >  2:467068/1‑99 (MQ=255)
                        aGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATAc                                                                             <  2:572262/100‑1 (MQ=255)
                        aGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAGCATAGTCACTTGATGTGACTATGATCCGATTAATAc                                                                             >  1:1032495/1‑100 (MQ=255)
                         gCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAACATAGTCACTTGATGTGACTATGATCCGATTAATAct                                                                            >  1:338344/1‑100 (MQ=255)
                          ccTGTTCAGCTTCTCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActc                                                                           <  1:1490962/100‑1 (MQ=255)
                          ccTGTTCAGCTTCACCCGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTCGTCACCCGGACCATAGTCACTTGATGTGACTATGATCCGATTAATAc                                                                             >  2:1148935/1‑98 (MQ=255)
                            tGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActctc                                                                         <  2:1645373/100‑1 (MQ=255)
                               tCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTcc                                                                      <  2:714177/100‑1 (MQ=255)
                                  gCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCt                                                                   >  2:114351/1‑100 (MQ=255)
                                  gCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCt                                                                   >  1:297717/1‑100 (MQ=255)
                                  gCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCt                                                                   >  1:298986/1‑100 (MQ=255)
                                     tCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTAcn                                                                <  2:830005/100‑2 (MQ=255)
                                     tCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACg                                                                <  2:338340/100‑1 (MQ=255)
                                       gCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGCTACGATGAATACTCTCTCCGCTACGc                                                               >  2:598617/1‑99 (MQ=255)
                                       gCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCa                                                              >  1:353430/1‑100 (MQ=255)
                                       gCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCa                                                              >  1:1580631/1‑100 (MQ=255)
                                       gCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCAGCTACGCa                                                              >  2:576666/1‑100 (MQ=255)
                                       gCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAACATAGTGAATTGATGTGACTATGATCCGATTAATACTCTCTCTGCTACg                                                                >  2:1030630/1‑98 (MQ=255)
                                        ccAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAg                                                             >  1:878101/1‑100 (MQ=255)
                                        ccAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAg                                                             >  1:2495822/1‑100 (MQ=255)
                                           gTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAgtgt                                                          <  1:1167753/100‑1 (MQ=255)
                                            tttGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgtt                                                         <  2:316672/100‑1 (MQ=255)
                                              tGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgttgt                                                       <  1:1112770/100‑1 (MQ=255)
                                               gTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTa                                                      <  2:468981/100‑1 (MQ=255)
                                               gTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTa                                                      <  1:395121/100‑1 (MQ=255)
                                                ttCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAg                                                     >  2:1901299/1‑100 (MQ=255)
                                                ttCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAg                                                     >  2:2149483/1‑100 (MQ=255)
                                                 tCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGa                                                    >  2:1019429/1‑100 (MQ=255)
                                                  cGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGAt                                                   >  1:2242351/1‑100 (MQ=255)
                                                  cGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGAGCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGAt                                                   >  2:1542298/1‑100 (MQ=255)
                                                  cGTTAAGCAGCAATAATTACCCCGGTTGTCACACGGATAATAGTCACTTGATGTGACTATGATCCGATTAATACTATCTCCGCTACGAAGTGTTGTAGAt                                                   >  2:1542269/1‑100 (MQ=255)
                                                   gTTAAGCAGCAATGATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGAGCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATc                                                  <  2:68441/100‑1 (MQ=255)
                                                   gTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATc                                                  >  1:377648/1‑100 (MQ=255)
                                                   gTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATc                                                  <  2:323252/100‑1 (MQ=255)
                                                   gTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATc                                                  >  1:2048126/1‑100 (MQ=255)
                                                   gTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGAGCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATc                                                  >  2:1010493/1‑100 (MQ=255)
                                                       agcagcAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAAtt                                              >  2:328040/1‑100 (MQ=255)
                                                       agcagcAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAAtt                                              >  2:1696877/1‑100 (MQ=255)
                                                       agcagcAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAAtt                                              >  2:779610/1‑100 (MQ=255)
                                                       accagcaATAATTACCCCGGTTGTCACCCGGGTCATAGTCACATGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAAtt                                              >  1:1734050/3‑100 (MQ=255)
                                                           gcaATACTTACCCCGGTTGTCACCCGGAACATAGTCACTTGATGAGACTATTATCCGACTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTgagc                                          >  2:444516/1‑100 (MQ=255)
                                                           gcaATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTTTGGTAGATCAGGTgcgc                                          >  1:2041626/1‑100 (MQ=255)
                                                            caATAATTACACCGGTTGTCACCCGGATCATAGTCACTTGATGTGAGTATGATCCGTTTAATACTCTCTCCGCTACGAAGTTTTGTAGAGCAATTGCGCa                                         >  2:2459578/1‑100 (MQ=255)
                                                                 atTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATc                                    >  1:2384334/1‑100 (MQ=255)
                                                                 atTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATc                                    >  1:520799/1‑100 (MQ=255)
                                                                     ccccGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCACACTATCATTg                                <  2:894502/100‑1 (MQ=255)
                                                                      cccGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGa                               <  1:2047820/100‑1 (MQ=255)
                                                                       gcGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTATGCAGTGTTGTAGATCAATTGCGCACTATCATTGaa                              <  2:364829/99‑1 (MQ=255)
                                                                        cGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGaaa                             <  1:101809/100‑1 (MQ=255)
                                                                               cACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAc                      <  1:1435245/100‑1 (MQ=255)
                                                                                aCCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAcc                     <  1:767798/100‑1 (MQ=255)
                                                                                aCCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAcc                     <  1:435183/100‑1 (MQ=255)
                                                                                 cccGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCTGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCt                    >  2:1810809/1‑100 (MQ=255)
                                                                                   cGGATCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGCAGATCAATTGCGCACTATCATTGAAATAATTACCTGc                  >  1:495381/1‑100 (MQ=37)
                                                                                    ggATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCt                 <  1:1667395/100‑1 (MQ=255)
                                                                                    ggAGCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATAGCGCACTATCATTGAAATAATTACCTGCt                 >  1:1652673/1‑100 (MQ=38)
                                                                                     gATCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTa                >  1:681570/1‑100 (MQ=38)
                                                                                       tCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGt              <  2:2495807/100‑1 (MQ=255)
                                                                                        cATAGTCGCTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTg             >  1:1340392/1‑100 (MQ=255)
                                                                                         aTAGTCACATCAAGTGACTATCATCCGATTAATACTCTCTCCACTAAGCATTGCTGTAGATCAATTGCGCACCATCACTGAAATAATTAGCTGCTACTGa            >  2:627950/1‑100 (MQ=255)
                                                                                          tAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGat           <  2:1259238/100‑1 (MQ=255)
                                                                                             tctcTTGATGTGTCTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCTCACTATCATTGAAATAATTACCTGCTAGTGattat        <  2:2178269/97‑1 (MQ=255)
                                                                                             tCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGattat        <  2:1832140/100‑1 (MQ=255)
                                                                                               aCTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTAttt      >  2:2426630/1‑100 (MQ=255)
                                                                                                  tGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCaa   >  1:1496272/1‑100 (MQ=255)
                                                                                                  tGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCaa   >  2:1508931/1‑100 (MQ=255)
                                                                                                   gATGTGACTATGATCCGATTTATACTCTCTCCGCTACGCAGTGTTGTAGTTCAAGTGCGCACTATCGTTGAAATAATTACCTGCTAGTGATTATTTCAAc  <  2:1669308/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
ATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAC  >  NC_000913/3657502‑3657700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: