Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,669,920	A→G	100%	E110G (GAA→GGA)	treF →	cytoplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,669,920	0	A	G	98.4%	323.2 / ‑4.9	95	E110G (GAA→GGA)	treF	cytoplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (42/51);  total (42/53)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCT  >  NC_000913/3669824‑3670016                                                                                                 |                                                                                                 ttCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGgaaa                                                                                               <  1:1608309/100‑1 (MQ=255) ttCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGgaaa                                                                                               <  2:879797/100‑1 (MQ=255)   cccGACTGCGCACCGAAAATGGACCCGCTGGATATCCTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAAcc                                                                                             <  1:1802580/100‑1 (MQ=255)    ccGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCa                                                                                            >  2:889429/1‑100 (MQ=255)          gcgcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTg                                                                                      <  1:2538352/100‑1 (MQ=255)            gcACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGc                                                                                    <  1:1572530/100‑1 (MQ=255)             cACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAGAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCt                                                                                   >  2:1696430/1‑100 (MQ=255)               ccTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGc                                                                                 >  2:1896195/1‑100 (MQ=255)               ccTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGc                                                                                 >  2:393815/1‑100 (MQ=255)               ccTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGc                                                                                 >  1:2338224/1‑100 (MQ=255)               ccTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGc                                                                                 >  2:1756206/1‑100 (MQ=255)                 tAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCg                                                                               >  1:200868/1‑100 (MQ=255)                 tAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTGCTGGCTGCCg                                                                               >  2:1678110/1‑100 (MQ=255)                  aaaaTGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCgg                                                                              >  2:914807/1‑100 (MQ=255)                   aaaTGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGa                                                                             <  1:255286/100‑1 (MQ=255)                      tGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGt                                                                          <  2:813832/100‑1 (MQ=255)                       ggACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTc                                                                         <  2:315178/100‑1 (MQ=255)                       ggACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTc                                                                         <  2:119670/100‑1 (MQ=255)                       ggACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTc                                                                         <  1:661573/100‑1 (MQ=255)                        gACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCt                                                                        <  1:2438536/100‑1 (MQ=255)                        gACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCt                                                                        <  1:78157/100‑1 (MQ=255)                        gACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCt                                                                        <  2:1138966/100‑1 (MQ=255)                         aCCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCt                                                                        <  1:1180838/99‑1 (MQ=255)                          cccGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTAc                                                                      >  2:2474434/1‑100 (MQ=255)                          cccGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTAc                                                                      >  1:2462238/1‑100 (MQ=255)                           ccGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACt                                                                     >  1:1642814/1‑100 (MQ=255)                           ccGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACt                                                                     >  1:813622/1‑100 (MQ=255)                            cGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTc                                                                    >  1:2024207/1‑100 (MQ=255)                            cGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTc                                                                    >  1:1234055/1‑100 (MQ=255)                             gCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTcc                                                                   <  2:636739/100‑1 (MQ=255)                               tGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAg                                                                 <  1:1113876/100‑1 (MQ=255)                                ggATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGc                                                                >  1:1528501/1‑100 (MQ=255)                                 gATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCg                                                               <  1:929836/100‑1 (MQ=255)                                   tatCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAg                                                             <  2:1528490/100‑1 (MQ=255)                                   tatCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAg                                                             >  1:1667198/1‑100 (MQ=255)                                     tCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgta                                                           >  2:1623697/1‑100 (MQ=255)                                     tCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgta                                                           <  1:1901489/100‑1 (MQ=255)                                     tCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgta                                                           <  2:919104/100‑1 (MQ=255)                                      cTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtat                                                          >  1:1349875/1‑100 (MQ=255)                                       ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAATCACTTCTGGCTGACGGAGGTCTACTCCAGCGAgtatg                                                         <  2:2012194/100‑1 (MQ=255)                                       ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                         >  1:963099/1‑100 (MQ=255)                                       ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                         <  1:1578809/100‑1 (MQ=255)                                       ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                         <  2:1629699/100‑1 (MQ=255)                                       ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                         <  1:61212/100‑1 (MQ=255)                                       ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                         >  1:1213847/1‑100 (MQ=255)                                       ttAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatg                                                         <  2:2094467/100‑1 (MQ=255)                                         aaTCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAgtatgta                                                       <  1:361522/100‑1 (MQ=255)                                           tCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATc                                                     <  1:971822/100‑1 (MQ=255)                                           tCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATc                                                     <  1:2444227/100‑1 (MQ=255)                                            ccGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCg                                                    <  1:1467639/100‑1 (MQ=255)                                             cGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCgg                                                   >  1:1194166/1‑100 (MQ=255)                                                 aCCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGAccc                                               <  2:1384368/100‑1 (MQ=255)                                                  ccGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCg                                              <  1:716613/100‑1 (MQ=255)                                                  ccGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCg                                              >  2:233772/1‑100 (MQ=255)                                                   cGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGc                                             <  1:889437/100‑1 (MQ=255)                                                   cGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGc                                             >  1:1619256/1‑100 (MQ=255)                                                    gTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCa                                            >  1:2540268/1‑100 (MQ=255)                                                            cgcCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTccc                                    <  1:1148840/100‑1 (MQ=255)                                                              cggTCACCGTTATTTAGCCTTGCGCAAGGTTGTTAGAAACCACTTCTAGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTccat                                   <  2:378876/97‑3 (MQ=255)                                                              ccGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTATGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTccc                                    <  2:372820/98‑1 (MQ=255)                                                               cGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGa                                 <  1:2444844/100‑1 (MQ=255)                                                               cGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGa                                 >  1:1071984/1‑100 (MQ=255)                                                               cGTCATCGTGATATTGACTTGCGCAAGTTTGTTAGAAACCAATTCTAGCTGCCGGAGCTCTACTGCAGCGAGTACGGATCGAAACCGCAAAAtaacct                                   <  2:506601/98‑6 (MQ=255)                                                                gTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGaa                                <  1:2538921/100‑1 (MQ=255)                                                                gTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGaa                                <  1:2305130/100‑1 (MQ=255)                                                                gTCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGaa                                <  2:1825097/100‑1 (MQ=255)                                                                 tCATCGTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGaaa                               >  2:1946912/1‑100 (MQ=255)                                                                      gTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGCAAGAGCa                          >  2:424597/1‑100 (MQ=255)                                                                      gTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGc                           >  2:959600/1‑99 (MQ=255)                                                                      gTGATTTTGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCa                          <  1:146173/100‑1 (MQ=255)                                                                      gTGATTTTGACGTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGACGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGc                           <  2:2226302/99‑1 (MQ=255)                                                                          ttttGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATc                      <  1:1989477/100‑1 (MQ=255)                                                                          ttttGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGCTCTACTCCCGCGAGTATGTAGCGGAGCCGCAAAATTCCCTGAAAGAGCATATc                      >  2:1468413/1‑100 (MQ=255)                                                                            ttGACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCgc                    <  2:1807530/100‑2 (MQ=255)                                                                              gACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGAcc                  <  1:1696442/100‑1 (MQ=255)                                                                              gACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGAcc                  >  2:545411/1‑100 (MQ=255)                                                                              gACTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGAcc                  <  1:236609/100‑1 (MQ=255)                                                                               aCTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCa                 <  2:1927310/100‑1 (MQ=255)                                                                                cTTGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAg                >  2:1050807/1‑100 (MQ=255)                                                                                 ttGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGc               <  2:123441/100‑1 (MQ=255)                                                                                 ttGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGc               <  2:123420/100‑1 (MQ=255)                                                                                  tGCGCAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCt              >  2:411467/1‑100 (MQ=255)                                                                                    cgcAAGTTTGTTGGAAACCACTTCTGGCTGCAGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCtg             <  2:63580/99‑1 (MQ=255)                                                                                     gcAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCtgtg           <  1:235694/100‑1 (MQ=255)                                                                                      cAAGTTTGTTGGAAACCACTTTTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTgg          >  1:1956820/1‑100 (MQ=255)                                                                                      cAAGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTgg          >  1:173976/1‑100 (MQ=255)                                                                                        aGTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGcc        >  1:398315/1‑100 (MQ=255)                                                                                         gTTTGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCg       >  2:2164442/1‑100 (MQ=255)                                                                                          tttGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCgg      >  1:831186/1‑100 (MQ=255)                                                                                          tttGTTGGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCgg      <  1:959608/100‑1 (MQ=255)                                                                                           ttgttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCgg      <  2:1634180/99‑1 (MQ=255)                                                                                           ttgttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGt     <  2:686206/100‑1 (MQ=255)                                                                                            tgttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTg    >  2:1849687/1‑100 (MQ=255)                                                                                            tgttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTg    >  2:45530/1‑100 (MQ=255)                                                                                            tgttgGAAACCAATTCTCGCTGCCGGAGTTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTg    <  2:165580/100‑1 (MQ=255)                                                                                              ttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGCCCCGCAAAATTCCCTGAAAGAGCCTATCGACCAGCTGTGGCCGGTGCt  >  2:1138380/1‑100 (MQ=255)                                                                                              ttgGAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCt  >  1:1466478/1‑100 (MQ=255)                                                                                                 |                                                                                                 TTCCCGACTGCGCACCTAAAATGGACCCGCTGGATATCTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAAGTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGCATATCGACCAGCTGTGGCCGGTGCT  >  NC_000913/3669824‑3670016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑