Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,708,410 T→A 100% intergenic (‑705/+206) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,708,4100TA93.1% 240.4 / 6.1 84intergenic (‑705/+206)dppA/proKdipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base A (31/47);  total (33/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTG  >  NC_000913/3708314‑3708504
                                                                                                |                                                                                              
gCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCa                                                                                             >  1:2304925/1‑100 (MQ=255)
  acaTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCaaa                                                                                           >  1:924342/1‑100 (MQ=255)
   caTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCaaaa                                                                                          >  2:387368/1‑100 (MQ=255)
   caTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCaaaa                                                                                          <  2:2326089/100‑1 (MQ=255)
   caTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCaaaa                                                                                          >  2:2091399/1‑100 (MQ=255)
     tttGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCaaaaaa                                                                                        <  1:810236/100‑1 (MQ=255)
      ttGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAg                                                                                       <  1:427524/100‑1 (MQ=255)
       tGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGc                                                                                      >  1:963207/1‑100 (MQ=255)
        gCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGcc                                                                                     <  1:2469282/100‑1 (MQ=255)
         ccTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGccn                                                                                    <  1:2467681/100‑2 (MQ=255)
          cTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGACCCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTg                                                                                   >  2:1616370/1‑100 (MQ=255)
           tGATGCGACGCTGACGCGTATTATCAGGCCTACGTCGAGTCGAACCTCCCTGACCCCGGAGGTTTTCATCCTCATGAGCTGCAGAAGAAAAAAAGGCTg                                                                                   >  1:511266/1‑99 (MQ=21)
            gATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCt                                                                                 <  2:345742/100‑1 (MQ=255)
              tGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCg                                                                               >  1:2125501/1‑100 (MQ=255)
              tGCGACGATGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCg                                                                               >  1:1346548/1‑100 (MQ=255)
                 gACGCTGACGCGTCTTATCAGGCCTACGTCGCGTCGAACCTCCCTGCCCCCGGAGGTTCTCCTCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTg                                                                            <  1:2559689/100‑1 (MQ=255)
                 gACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTg                                                                            <  1:462982/100‑1 (MQ=255)
                 gACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTg                                                                            >  1:2070174/1‑100 (MQ=255)
                  aCGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAAAAACAAAAAAGCCTGCTCGTTGa                                                                           <  2:74487/100‑1 (MQ=255)
                   cGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAg                                                                          <  1:1190089/100‑1 (MQ=255)
                   cGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAg                                                                          >  1:868465/1‑100 (MQ=255)
                   cGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAg                                                                          >  1:81275/1‑100 (MQ=255)
                    gCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGc                                                                         <  1:1569377/100‑1 (MQ=255)
                     cTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCa                                                                        >  2:160523/1‑100 (MQ=255)
                      gtcagcgTCGCATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCCTGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAg                                                                       <  2:1364274/96‑1 (MQ=14)
                         cgcgTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCt                                                                    <  2:996942/100‑1 (MQ=255)
                         cgcgTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCAACGAGCAGGCt                                                                    <  1:1080719/100‑1 (MQ=17)
                           cgccTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCttt                                                                  <  2:1361779/97‑1 (MQ=255)
                            gTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCtttt                                                                 >  2:62015/1‑100 (MQ=255)
                             tattATCAGGCCTACGTCGAGTCGAACTTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCAACGAGCAGGCt                                                                    <  2:1056902/94‑1 (MQ=14)
                             tCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTc                                                                <  1:121582/100‑1 (MQ=255)
                              cTTATCAGGCCTACGTCGAGTTGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCg                                                               <  1:1934901/100‑1 (MQ=255)
                               ttATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGa                                                              <  1:1681026/100‑1 (MQ=255)
                                tATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGaa                                                             >  1:640373/1‑100 (MQ=255)
                                tATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCAACGAGCAGGCttttttgc                                                             <  1:2341285/100‑5 (MQ=14)
                                tATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCAACGAGCAGGCttttttg                                                              <  2:893524/99‑4 (MQ=14)
                                 aTCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAt                                                            >  2:2019917/1‑100 (MQ=255)
                                 aTCAGGCCTACGTCGACTCGAACCTCCCTGCCCCCAGAGGTTCTCATCCTCATGAGCGGCAGAAGCAAAAAAGCCTGCTCAACGAGCAGGCttttttgct                                                            <  2:1172537/100‑6 (MQ=14)
                                  tCAGGCCTACGTCGATTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAtt                                                           <  2:1762932/100‑1 (MQ=255)
                                    aGGCGTATGTCGAGTCGAACCTCCCTGCCCATGTAGGTTCTCATCCTCATGAGCTGTAGAAGAAAAATAGCATTCTCGTTGCGCAGGCTTTTCGAAtt                                                           <  2:1901493/98‑1 (MQ=11)
                                    aGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTg                                                         >  1:1063932/1‑100 (MQ=255)
                                    aGGCCTACGTCGAGTCGAACCTCCCTGCACCCGAAGTTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCAACGAGCAGGCttttttgcttct                                                         <  2:914396/100‑9 (MQ=14)
                                     ggCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTgg                                                        <  2:19444/100‑1 (MQ=255)
                                      gCCTAGGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTgg                                                        <  2:2304911/99‑1 (MQ=18)
                                      ccttccGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCAACCTCATCAGCTGCATATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCCAATTTg                                                         <  2:511260/93‑1 (MQ=11)
                                       ccTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTc                                                      >  2:1950737/1‑100 (MQ=25)
                                       ccTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTc                                                      <  1:936358/100‑1 (MQ=25)
                                        cTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCg                                                     >  1:1125235/1‑100 (MQ=25)
                                        cTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCg                                                     <  1:2385339/100‑1 (MQ=25)
                                           cGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTg                                                  <  2:1383585/100‑1 (MQ=14)
                                            gtcgagtcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGa                                                 <  2:2538713/100‑1 (MQ=11)
                                            gtcgagtcgaACCTCACTCCCCCCGGAGGTTCTCATCCCCATGCGCTGCAGAAGCACAAACGTCTTCTCGTTGAGCACGCTTTTCGAATTTGGTctg                                                    >  2:2423296/1‑95 (MQ=11)
                                                         ncccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTc                                    >  1:2538571/2‑100 (MQ=18)
                                                           cccgCCCCCGGAGGTTCTCATCCTCATGAGCTACAAATGCAAAAAAGCCTGCTCGCTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGc                                  >  1:155171/4‑100 (MQ=11)
                                                            cTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGcc                                 <  1:1060463/100‑1 (MQ=255)
                                                            cTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGcc                                 <  1:1842037/100‑1 (MQ=255)
                                                              gCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCAc                               <  1:1396575/100‑1 (MQ=255)
                                                                  ccGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCg                           >  1:598482/1‑100 (MQ=255)
                                                                   cGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGt                          <  1:589404/100‑1 (MQ=255)
                                                                   cGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGt                          <  2:307262/100‑1 (MQ=255)
                                                                     gAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTgg                        <  1:2091413/100‑1 (MQ=255)
                                                                     gAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTgg                        <  1:1333546/100‑1 (MQ=255)
                                                                          tctcATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGc                   <  1:1839935/100‑1 (MQ=255)
                                                                            tcATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTAGTCGCTACTTCGTGTCTCGc                   >  2:89809/1‑98 (MQ=14)
                                                                            tcATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGccc                 <  2:1763265/100‑1 (MQ=255)
                                                                            tcATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGccc                 <  1:362392/100‑1 (MQ=255)
                                                                            tcATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGcc                  <  1:362923/99‑1 (MQ=255)
                                                                             cATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCt                >  2:248385/1‑100 (MQ=255)
                                                                             cATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCt                >  2:374930/1‑100 (MQ=255)
                                                                               tCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGc              >  2:2358467/1‑100 (MQ=255)
                                                                                 cTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCgg            >  1:131136/1‑100 (MQ=255)
                                                                                  tCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCggg           >  1:1531560/1‑100 (MQ=255)
                                                                                   cATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCggtc          <  1:2375652/100‑3 (MQ=255)
                                                                                   cATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGc          >  2:1910578/1‑100 (MQ=255)
                                                                                    aTGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGcc         <  1:1310525/100‑1 (MQ=255)
                                                                                    aTGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGcc         <  1:825820/100‑1 (MQ=255)
                                                                                     tGAGCTGGAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCg        <  2:1753346/100‑1 (MQ=255)
                                                                                     tGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTg               >  2:282142/1‑93 (MQ=255)
                                                                                     tGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCg        <  1:1661348/100‑1 (MQ=255)
                                                                                     tGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCg        <  1:1616381/100‑1 (MQ=255)
                                                                                      gAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGt       <  1:1142846/100‑1 (MQ=255)
                                                                                      gAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGt       <  1:2358482/100‑1 (MQ=255)
                                                                                           gCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTg  >  1:101557/1‑100 (MQ=255)
                                                                                           gCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTg  >  1:1568069/1‑100 (MQ=255)
                                                                                                |                                                                                              
GCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTG  >  NC_000913/3708314‑3708504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑