Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,708,427 G→A 16.6% intergenic (‑722/+189) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,708,4270GA16.6% 220.5 / 31.5 96intergenic (‑722/+189)dppA/proKdipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base G (37/43);  new base A (10/6);  total (47/49)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCG  >  NC_000913/3708328‑3708525
                                                                                                   |                                                                                                  
tGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCg                                                                                                    >  1:2125501/1‑100 (MQ=255)
tGCGACGATGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCg                                                                                                    >  1:1346548/1‑100 (MQ=255)
   gACGCTGACGCGTCTTATCAGGCCTACGTCGCGTCGAACCTCCCTGCCCCCGGAGGTTCTCCTCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTg                                                                                                 <  1:2559689/100‑1 (MQ=255)
   gACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTg                                                                                                 >  1:2070174/1‑100 (MQ=255)
   gACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTg                                                                                                 <  1:462982/100‑1 (MQ=255)
    aCGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAAAAACAAAAAAGCCTGCTCGTTGa                                                                                                <  2:74487/100‑1 (MQ=255)
     cGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAg                                                                                               <  1:1190089/100‑1 (MQ=255)
     cGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAg                                                                                               >  1:81275/1‑100 (MQ=255)
     cGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAg                                                                                               >  1:868465/1‑100 (MQ=255)
      gCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGc                                                                                              <  1:1569377/100‑1 (MQ=255)
       cTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCa                                                                                             >  2:160523/1‑100 (MQ=255)
        gtcagcgTCGCATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCCTGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAg                                                                                            <  2:1364274/96‑1 (MQ=14)
           cgcgTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCt                                                                                         <  2:996942/100‑1 (MQ=255)
           cgcgTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCAACGAGCAGGCt                                                                                         <  1:1080719/100‑1 (MQ=17)
             cgccTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCttt                                                                                       <  2:1361779/97‑1 (MQ=255)
              gTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCtttt                                                                                      >  2:62015/1‑100 (MQ=255)
               tattATCAGGCCTACGTCGAGTCGAACTTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCAACGAGCAGGCt                                                                                         <  2:1056902/94‑1 (MQ=14)
               tCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTc                                                                                     <  1:121582/100‑1 (MQ=255)
                cTTATCAGGCCTACGTCGAGTTGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCg                                                                                    <  1:1934901/100‑1 (MQ=255)
                 ttATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGa                                                                                   <  1:1681026/100‑1 (MQ=255)
                  tATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGaa                                                                                  >  1:640373/1‑100 (MQ=255)
                  tATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCAACGAGCAGGCttttttgc                                                                                  <  1:2341285/100‑5 (MQ=14)
                  tATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCAACGAGCAGGCttttttg                                                                                   <  2:893524/99‑4 (MQ=14)
                   aTCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAt                                                                                 >  2:2019917/1‑100 (MQ=255)
                   aTCAGGCCTACGTCGACTCGAACCTCCCTGCCCCCAGAGGTTCTCATCCTCATGAGCGGCAGAAGCAAAAAAGCCTGCTCAACGAGCAGGCttttttgct                                                                                 <  2:1172537/100‑6 (MQ=14)
                    tCAGGCCTACGTCGATTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAtt                                                                                <  2:1762932/100‑1 (MQ=255)
                      aGGCGTATGTCGAGTCGAACCTCCCTGCCCATGTAGGTTCTCATCCTCATGAGCTGTAGAAGAAAAATAGCATTCTCGTTGCGCAGGCTTTTCGAAtt                                                                                <  2:1901493/98‑1 (MQ=11)
                      aGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTg                                                                              >  1:1063932/1‑100 (MQ=255)
                      aGGCCTACGTCGAGTCGAACCTCCCTGCACCCGAAGTTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCAACGAGCAGGCttttttgcttct                                                                              <  2:914396/100‑9 (MQ=14)
                       ggCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTgg                                                                             <  2:19444/100‑1 (MQ=255)
                        gCCTAGGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTgg                                                                             <  2:2304911/99‑1 (MQ=18)
                        ccttccGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCAACCTCATCAGCTGCATATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCCAATTTg                                                                              <  2:511260/93‑1 (MQ=11)
                         ccTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTc                                                                           <  1:936358/100‑1 (MQ=25)
                         ccTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTc                                                                           >  2:1950737/1‑100 (MQ=25)
                          cTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCg                                                                          >  1:1125235/1‑100 (MQ=25)
                          cTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCg                                                                          <  1:2385339/100‑1 (MQ=25)
                             cGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTg                                                                       <  2:1383585/100‑1 (MQ=14)
                              gtcgagtcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGa                                                                      <  2:2538713/100‑1 (MQ=11)
                              gtcgagtcgaACCTCACTCCCCCCGGAGGTTCTCATCCCCATGCGCTGCAGAAGCACAAACGTCTTCTCGTTGAGCACGCTTTTCGAATTTGGTctg                                                                         >  2:2423296/1‑95 (MQ=11)
                                           ncccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTc                                                         >  1:2538571/2‑100 (MQ=18)
                                             cccgCCCCCGGAGGTTCTCATCCTCATGAGCTACAAATGCAAAAAAGCCTGCTCGCTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGc                                                       >  1:155171/4‑100 (MQ=11)
                                              cTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGcc                                                      <  1:1842037/100‑1 (MQ=255)
                                              cTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGcc                                                      <  1:1060463/100‑1 (MQ=255)
                                                gCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCAc                                                    <  1:1396575/100‑1 (MQ=255)
                                                    ccGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCg                                                >  1:598482/1‑100 (MQ=255)
                                                     cGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGt                                               <  2:307262/100‑1 (MQ=255)
                                                     cGGAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGt                                               <  1:589404/100‑1 (MQ=255)
                                                       gAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTgg                                             <  1:2091413/100‑1 (MQ=255)
                                                       gAGGTTCTCATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTgg                                             <  1:1333546/100‑1 (MQ=255)
                                                            tctcATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGc                                        <  1:1839935/100‑1 (MQ=255)
                                                              tcATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTAGTCGCTACTTCGTGTCTCGc                                        >  2:89809/1‑98 (MQ=14)
                                                              tcATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGccc                                      <  1:362392/100‑1 (MQ=255)
                                                              tcATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGccc                                      <  2:1763265/100‑1 (MQ=255)
                                                              tcATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGcc                                       <  1:362923/99‑1 (MQ=255)
                                                               cATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCt                                     >  2:248385/1‑100 (MQ=255)
                                                               cATCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCt                                     >  2:374930/1‑100 (MQ=255)
                                                                 tCCTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGc                                   >  2:2358467/1‑100 (MQ=255)
                                                                   cTCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCgg                                 >  1:131136/1‑100 (MQ=255)
                                                                    tCATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCggg                                >  1:1531560/1‑100 (MQ=255)
                                                                     cATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCggtc                               <  1:2375652/100‑3 (MQ=255)
                                                                     cATGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGc                               >  2:1910578/1‑100 (MQ=255)
                                                                      aTGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGcc                              <  1:825820/100‑1 (MQ=255)
                                                                      aTGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGcc                              <  1:1310525/100‑1 (MQ=255)
                                                                       tGAGCTGGAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCg                             <  2:1753346/100‑1 (MQ=255)
                                                                       tGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTg                                    >  2:282142/1‑93 (MQ=255)
                                                                       tGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCg                             <  1:1661348/100‑1 (MQ=255)
                                                                       tGAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCg                             <  1:1616381/100‑1 (MQ=255)
                                                                        gAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGt                            <  1:1142846/100‑1 (MQ=255)
                                                                        gAGCTGCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGt                            <  1:2358482/100‑1 (MQ=255)
                                                                             gCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTg                       >  1:1568069/1‑100 (MQ=255)
                                                                             gCAGAAGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTg                       >  1:101557/1‑100 (MQ=255)
                                                                                gaagCAAGAAACCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCGCTGCGGGCCGTTGCTg                       >  2:1340291/4‑97 (MQ=21)
                                                                                gaagCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGgcg                    <  2:1979025/97‑1 (MQ=255)
                                                                                 aattcgaaaaGCCTGCTCAACGAGCAGGCTTGTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCTTTGCTGgc                     >  1:1271710/7‑98 (MQ=25)
                                                                                 aagCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGAGAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGGGGGCGGTTGcggtcg                    >  2:1209198/3‑94 (MQ=21)
                                                                                   ttcgaaaaGCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCaa                 >  1:342584/5‑100 (MQ=25)
                                                                                    tcgaaaaGCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGGGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAAc                >  2:1707345/4‑100 (MQ=37)
                                                                                    cAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCTACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGgc                     >  2:1960491/1‑95 (MQ=255)
                                                                                    cAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCCAc                >  1:2513643/1‑100 (MQ=255)
                                                                                     cgaaaaGCCTGCTCAACGAGCAGGCTTTTTGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGTCGCAACg               >  2:41853/3‑100 (MQ=25)
                                                                                     cgaaaaGCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACg               >  1:2544699/3‑100 (MQ=37)
                                                                                     cgaaaaGCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTAGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACg               >  1:2087993/3‑100 (MQ=25)
                                                                                     cgaaaaGCCTGCTCAACGAGAAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACg               >  2:2432097/3‑100 (MQ=37)
                                                                                     aaaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACg               >  1:1531819/1‑100 (MQ=255)
                                                                                     aaaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTGGCTGGCGCAACg               >  1:607300/1‑100 (MQ=255)
                                                                                      gaaaaGCCTGCTCAACGAGCAGGCTTTTCGACTTTGGTCGGTGATAGCGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGc                         >  2:1957546/2‑89 (MQ=25)
                                                                                      gaaaaGCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGt              >  2:1992617/2‑100 (MQ=37)
                                                                                      aaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGt              >  1:96953/1‑100 (MQ=255)
                                                                                        aaaGCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTtc            >  2:910456/1‑100 (MQ=37)
                                                                                           gCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGGGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTtctct         <  1:155234/100‑1 (MQ=255)
                                                                                           gCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTtctct         >  1:670230/1‑100 (MQ=255)
                                                                                            ccTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTAGTCGCTACTACGTGGGTGGCCCTGCGGGCGGTGGCTGGCGCACGGTtatctc        >  2:2467069/1‑100 (MQ=18)
                                                                                              tGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcgc      <  1:374934/100‑1 (MQ=255)
                                                                                              tGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcgc      >  1:1957771/1‑100 (MQ=255)
                                                                                              tGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcgc      >  2:858673/1‑100 (MQ=255)
                                                                                              tGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcgc      >  1:1910245/1‑100 (MQ=255)
                                                                                               gCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcgct     >  1:1435221/1‑100 (MQ=255)
                                                                                                  cGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcgcttcg  <  2:963199/100‑1 (MQ=255)
                                                                                                   |                                                                                                  
TGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCG  >  NC_000913/3708328‑3708525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: