Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,729,408 C→G 15.7% intergenic (‑37/+35) xylB ← / ← xylA xylulokinase/D‑xylose isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,729,4080CG15.7% 336.6 / 50.4 121intergenic (‑37/+35)xylB/xylAxylulokinase/D‑xylose isomerase
Reads supporting (aligned to +/- strand):  ref base C (59/42);  new base G (6/13);  total (66/55)
Fisher's exact test for biased strand distribution p-value = 4.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 128 total aligned reads displayed.

CCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCA  >  NC_000913/3729310‑3729507
                                                                                                   |                                                                                                   
cccTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTAcn                                                                                                    <  2:305074/100‑2 (MQ=255)
cccTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTAcc                                                                                                    >  2:2260197/1‑100 (MQ=255)
cccTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTAcc                                                                                                    <  2:1386397/100‑1 (MQ=255)
  cTGCTCGTTGAGCAAAATAACTTTTACGCCCGTGGTGCCAAGATGTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGa                                                                                                  >  1:921999/1‑100 (MQ=255)
   tGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGAt                                                                                                 <  1:667524/100‑1 (MQ=255)
       cGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAAcc                                                                                             >  1:1495584/1‑100 (MQ=255)
         ttGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCTCTACCGATAAcc                                                                                             >  2:117159/1‑98 (MQ=255)
          tgcgctaaaTTATTTTTACGAACAAGAAGCCAAGATCTATCCCGATATACATATCGATCGCCCCTTAAAAAAATGACCGGTATCCATAACGATAACCg                                                                                            >  2:51369/7‑98 (MQ=255)
          tGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTATCGGTAGCGATACCggg                                                                                          <  1:2445126/100‑1 (MQ=38)
          tGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTATCGGTAGCGATACCggg                                                                                          <  2:782319/100‑1 (MQ=38)
          tGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTATCGGTAGCGATACCggg                                                                                          <  1:748045/100‑1 (MQ=38)
          tGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCggg                                                                                          <  1:268887/100‑1 (MQ=255)
          tGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCggg                                                                                          <  2:2152652/100‑1 (MQ=255)
           gAGCAAAATAGCTTTTACGCCCGAGGTGCCAAGATCTAGCCCGAGATACATATCGATCGTTCCTTAGAAGAATGCCCGGTATCGCTACCGATAACCGGGc                                                                                         <  2:1689989/100‑1 (MQ=255)
            aGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTATCGGTAGCGATACCGGGca                                                                                        <  1:1875990/100‑2 (MQ=38)
            aGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTATCGGTAGCGATACCGGGc                                                                                         <  1:2520692/99‑1 (MQ=38)
            aGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGcc                                                                                        <  1:2389846/100‑1 (MQ=255)
            aGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGcc                                                                                        <  1:2153361/100‑1 (MQ=255)
            aGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGcc                                                                                        <  1:2124205/100‑1 (MQ=255)
             gCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTATCGGTAGCGATACCGGGcat                                                                                       <  1:2292775/100‑3 (MQ=37)
             gCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTATCGGTAGCGATACCGGGcat                                                                                       <  2:922512/100‑3 (MQ=37)
               aaaaTAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATCCCCGGTATCGCTACCGATAACCGGGCCAAc                                                                                     >  2:308000/1‑100 (MQ=255)
                ataTAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTATCGGTAGCGATACCGGGcatttt                                                                                    <  1:1964962/98‑6 (MQ=25)
                  aTAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTATCGGTAGCGATACCGGGcatttttt                                                                                  <  1:2500728/100‑8 (MQ=25)
                  aTAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTATCGGTAGCGATACCGGGcatttttt                                                                                  <  1:934044/100‑8 (MQ=25)
                  aTAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGa                                                                                  >  1:99762/1‑100 (MQ=255)
                    aaCTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACt                                                                                <  1:2378076/100‑1 (MQ=255)
                     aCTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTTATCGGTAGCGATACCGGGcatttttttaa                                                                               <  1:1417469/100‑11 (MQ=21)
                      cTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGc                                                                              >  1:1129685/1‑100 (MQ=255)
                      cTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGc                                                                              >  2:1955111/1‑100 (MQ=255)
                          tACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAg                                                                          <  2:2560335/100‑1 (MQ=255)
                           aCGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGt                                                                         >  1:2536236/1‑100 (MQ=255)
                              cccGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTa                                                                       >  2:1470544/1‑99 (MQ=255)
                              cccGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAg                                                                      >  2:1455198/1‑100 (MQ=255)
                                cGAGGTGCCAAAATCTATTCCGATATACATATCGATCGTTCATGAAAAAAATAGACGATATCGCTAGCGATAACCAAGCCAACGGACTGAACAgat                                                                        >  2:2557748/1‑94 (MQ=255)
                                cGAGGTGCAAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAg                                                                          <  2:2349272/94‑1 (MQ=255)
                                      gCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCCCAGTTAGCCGTTAtt                                                              >  2:439281/1‑100 (MQ=255)
                                      gCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAtt                                                              <  2:2497149/100‑1 (MQ=255)
                                      gCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAtt                                                              >  2:443771/1‑100 (MQ=255)
                                       ccAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAttt                                                             >  2:1720657/1‑100 (MQ=255)
                                        cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTAtt                                                              >  1:1201005/1‑98 (MQ=255)
                                        cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTg                                                            >  2:250587/1‑100 (MQ=255)
                                        cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTg                                                            >  2:1476511/1‑100 (MQ=255)
                                        cAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAAAGGACGGCACAGTTCGCCGTTACTTg                                                            >  2:2179233/1‑100 (MQ=255)
                                          aGATCTATCCCGATATACATATCGATCCTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTc                                                          <  1:439200/100‑1 (MQ=255)
                                           gATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCg                                                         <  2:2503037/100‑1 (MQ=255)
                                            aTCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGa                                                        >  1:1385578/1‑100 (MQ=255)
                                             tccctCCCGCTATACATATCGATCGATCCATAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCg                                                         <  2:23122/94‑1 (MQ=255)
                                               tATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTCCCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTccca                                                       >  2:448862/1‑95 (MQ=255)
                                               tATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACa                                                     >  1:1015604/1‑100 (MQ=255)
                                               tATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCAAACGGACTGCACAGTTAGCCGTTATTTGTCGAACa                                                     <  2:1051938/100‑1 (MQ=255)
                                                aTCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAg                                                    <  2:1674262/100‑1 (MQ=255)
                                                        ataCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGt                                            <  2:1877365/100‑1 (MQ=255)
                                                         taCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGtt                                           >  1:1119300/1‑100 (MQ=255)
                                                         taCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGtt                                           >  2:1181774/1‑100 (MQ=255)
                                                         taCATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGt                                            >  2:282149/1‑99 (MQ=255)
                                                           cATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGttt                                          >  2:2420299/1‑99 (MQ=255)
                                                           cATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTa                                         >  1:129046/1‑100 (MQ=255)
                                                           cATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCATTATTTGTCGAACAGATAATGGTTTa                                         >  1:1561231/1‑100 (MQ=255)
                                                             tatCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTAcc                                       >  1:1935097/1‑100 (MQ=255)
                                                             tatCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTAcc                                       >  2:2108803/1‑100 (MQ=255)
                                                              atcgatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCa                                      <  1:946924/100‑1 (MQ=255)
                                                               tcgatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAg                                     >  1:2329946/1‑100 (MQ=255)
                                                                cgatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGa                                    >  1:1677955/1‑100 (MQ=255)
                                                                 gatcgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGAt                                   >  1:2088630/1‑100 (MQ=255)
                                                                    cgTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGAttt                                 <  2:648078/99‑1 (MQ=255)
                                                                       tCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCa                             >  1:636495/1‑100 (MQ=255)
                                                                       tCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCa                             <  1:2561106/100‑1 (MQ=255)
                                                                        ccTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAg                            >  1:1010413/1‑100 (MQ=255)
                                                                         cTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAgt                           >  2:1339111/1‑100 (MQ=255)
                                                                         cTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTAACCAGATTTTCCAgt                           >  1:2517489/1‑100 (MQ=255)
                                                                          ttAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAgtt                          >  2:1381712/1‑100 (MQ=255)
                                                                           tAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAgttg                         <  2:2088616/100‑1 (MQ=255)
                                                                            aaaaaaaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAgttgt                        >  1:900486/1‑100 (MQ=255)
                                                                             aaaaaaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAgttgtt                       <  2:2537545/100‑1 (MQ=255)
                                                                             aaaaaaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAgttgtt                       >  1:1490918/1‑100 (MQ=255)
                                                                              aaaaaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTc                      >  1:837693/1‑100 (MQ=255)
                                                                                 ttggCCCGGTTATCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTg                   >  1:299015/4‑100 (MQ=37)
                                                                                 aaTGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTg                   <  2:1304362/100‑1 (MQ=255)
                                                                                  tggCCCGGTTATCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTgg                  >  1:713860/3‑100 (MQ=37)
                                                                                   tGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGc                 >  1:615140/1‑100 (MQ=255)
                                                                                    gCCCGGTTATCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCg                >  1:2274910/1‑100 (MQ=38)
                                                                                    gCCCGGTTATCGGTAGCGATACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCg                >  2:1424305/1‑100 (MQ=38)
                                                                                    gCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGca                >  2:2034024/1‑99 (MQ=255)
                                                                                    gCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGc                 <  1:2034038/99‑1 (MQ=255)
                                                                                       cGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGAcc             <  1:1381721/100‑1 (MQ=255)
                                                                                       cGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGAcc             <  1:503875/100‑1 (MQ=255)
                                                                                           tATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCAct          >  2:2064105/1‑100 (MQ=255)
                                                                                             tCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCActct        >  1:1389244/1‑100 (MQ=255)
                                                                                              cGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTg       >  1:1658127/1‑100 (MQ=255)
                                                                                              cGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTg       >  1:1375314/1‑100 (MQ=255)
                                                                                               gatACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGa      <  2:232108/98‑1 (MQ=255)
                                                                                               gCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGa      <  1:168578/100‑1 (MQ=255)
                                                                                               gCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGa      <  1:2029804/100‑1 (MQ=255)
                                                                                                cTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGAt     <  1:1925683/100‑1 (MQ=255)
                                                                                                cTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGAt     <  2:2001639/100‑1 (MQ=255)
                                                                                                 tACCGATAACCGGGCCAACGGACTGGACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGAt     <  2:384359/99‑1 (MQ=255)
                                                                                                 tACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATg    >  1:1231427/1‑100 (MQ=255)
                                                                                                  aCCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGc   <  2:1200997/100‑1 (MQ=255)
                                                                                                   ccGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCa  >  2:2237766/1‑100 (MQ=255)
                                                                                                   |                                                                                                   
CCCTGCTCGTTGAGCAAAATAACTTTTACGCCCGAGGTGCCAAGATCTATCCCGATATACATATCGATCGTTCCTTAAAAAAATGCCCGGTATCGCTACCGATAACCGGGCCAACGGACTGCACAGTTAGCCGTTATTTGTCGAACAGATAATGGTTTACCAGATTTTCCAGTTGTTCCTGGCGACCACTCTGATGCA  >  NC_000913/3729310‑3729507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: