BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	281,581	G→A	100%	R135W (CGG→TGG)	yagA ←	CP4‑6 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	281,581	0	G	A	100.0%	427.1 / NA	131	R135W (CGG→TGG)	yagA	CP4‑6 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (58/73); total (58/73)

Only 100 of 131 total aligned reads displayed.

TCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCC  >  NC_000913/281484‑281675

tcCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGc                                                                                              <  2:1819286/100‑1 (MQ=255)

tcCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTGAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTCCGAACCAGc                                                                                              >  2:140257/1‑100 (MQ=255)

 ccAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGc                                                                                              <  2:536558/99‑1 (MQ=255)

 ccAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGc                                                                                              <  1:1664153/99‑1 (MQ=255)

  cagcagGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTAGGCGCGTCGTGTTGGAACCAGc                                                                                              >  2:1144898/1‑98 (MQ=255)

  cagcagGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGGGCGGCGCGTCGTGTTCGAACCAGccc                                                                                            >  2:292186/1‑100 (MQ=255)

    gcagGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGTCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGt                                                                                          >  1:2538434/1‑100 (MQ=255)

    gcagGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGt                                                                                          <  2:1861120/100‑1 (MQ=255)

     cagGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTg                                                                                         <  2:1703732/100‑1 (MQ=255)

         gTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCgg                                                                                     >  2:1129137/1‑100 (MQ=255)

         gTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGGGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCgg                                                                                     >  2:1439031/1‑100 (MQ=255)

           gAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGa                                                                                   >  1:2194185/1‑100 (MQ=255)

             gCGGATGGCAGCGTCCACCGCCAAAAGGAAGGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCggg                                                                                    <  2:2019594/97‑1 (MQ=255)

             gCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAAt                                                                                 >  1:656980/1‑100 (MQ=255)

              cGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATg                                                                                >  1:2332417/1‑100 (MQ=255)

              cGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATg                                                                                <  2:574414/100‑1 (MQ=255)

                gATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCTATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGcc                                                                              <  2:1641769/100‑1 (MQ=255)

                    gCAGCGTCCACCGCCAAAAGGAACGTGGCCCTTCAAATCCATCTGCCAGCGGCGGTTCGGCGCGTCGTGTTCGCACCAGCCCGTGGCGGGAATGCCCg                                                                            >  2:1911311/1‑98 (MQ=255)

                    gCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGt                                                                          >  2:1245662/1‑100 (MQ=255)

                    gCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGt                                                                          <  2:2353623/100‑1 (MQ=255)

                      aGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCTAACCAGCCCGTGGCGGGAATGCCCGGTGa                                                                        <  2:807582/100‑1 (MQ=255)

                      aGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTAGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGa                                                                        >  2:1993282/1‑100 (MQ=255)

                        cGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAg                                                                      <  2:1022980/100‑1 (MQ=255)

                        cGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGCGGCGGGAATGCCCGGTGAAg                                                                      <  2:1561881/100‑1 (MQ=255)

                          tCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAgcg                                                                    >  1:691970/1‑100 (MQ=255)

                           ccgccGCCACAAGGATAGTGTCCCTAAAAATCCATCTGCCAAACGCGGTTCGGCGCGTCGTGTTCGATCCAGCCCGAGGCGGACATGCCCGGtcacgc                                                                     <  2:1751202/95‑6 (MQ=255)

                            cACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGcc                                                                  >  1:1944870/1‑100 (MQ=255)

                            cACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGcc                                                                  >  1:2396620/1‑100 (MQ=255)

                            cACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGcc                                                                  >  1:2453985/1‑100 (MQ=255)

                               cGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCgg                                                               >  1:1535059/1‑100 (MQ=255)

                                gCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgc                                                              <  1:112236/100‑1 (MQ=255)

                                gCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgc                                                              <  1:600998/100‑1 (MQ=255)

                                gCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgc                                                              >  2:922129/1‑100 (MQ=255)

                                 ccAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgca                                                             >  1:925895/1‑100 (MQ=255)

                                   aaaaGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgcagc                                                           <  2:925887/100‑1 (MQ=255)

                                   aaaaGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgcagc                                                           >  1:1295619/1‑100 (MQ=255)

                                    aaaGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgcagca                                                          <  1:560935/100‑1 (MQ=255)

                                    aaaGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGgcagca                                                          >  1:1506408/1‑100 (MQ=255)

                                     aaGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGTCCGGTGAAGCGCCCGGcagcag                                                         <  2:1484403/100‑1 (MQ=255)

                                      aGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAgg                                                        <  1:1180690/100‑1 (MQ=255)

                                       ggAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGc                                                       >  2:796109/1‑100 (MQ=255)

                                       ggAAAGTGGCCCTTAAAATCCATCTGCCAGAGACGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGc                                                       >  1:2427867/1‑100 (MQ=255)

                                        gAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGcc                                                      <  1:1778109/100‑1 (MQ=255)

                                               gCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCgg                                               <  1:1144550/100‑1 (MQ=255)

                                                cccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCggg                                              <  2:1924273/100‑1 (MQ=255)

                                                cccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCggg                                              <  1:837576/100‑1 (MQ=255)

                                                 ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                             >  1:1901478/1‑100 (MQ=255)

                                                 ccTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGc                                             <  1:1688210/100‑1 (MQ=255)

                                                 ccTTAAAAGCCATCTGCCAGAGGCGGTCGGGCGCGGCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCggg                                              <  2:2341947/99‑1 (MQ=255)

                                                  cTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGcc                                            <  1:451173/100‑1 (MQ=255)

                                                   ttAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGTATGCCCGGTGATGCGCCCGGCAGCAGGCCATGGCGGGc                                             <  2:33633/98‑1 (MQ=255)

                                                   ttAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCa                                           <  1:1838159/100‑1 (MQ=255)

                                                    tAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCAt                                          >  1:1822112/1‑100 (MQ=255)

                                                      aaaTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCa                                        <  2:1602016/100‑1 (MQ=255)

                                                      aaaTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCa                                        >  2:1551681/1‑100 (MQ=255)

                                                       aaTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAg                                       >  2:1906507/1‑100 (MQ=255)

                                                        aTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAgg                                      <  1:1781033/100‑1 (MQ=255)

                                                        aTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAgg                                      >  1:2357860/1‑100 (MQ=255)

                                                        aTCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAgg                                      <  2:2332403/100‑1 (MQ=255)

                                                          ccATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGtt                                    <  2:448505/100‑1 (MQ=255)

                                                          ccATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGtt                                    <  2:168580/100‑1 (MQ=255)

                                                            aTCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTAt                                  >  2:1355358/1‑100 (MQ=255)

                                                             tCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATg                                 <  1:988957/100‑1 (MQ=255)

                                                             tCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATg                                 <  2:2501220/100‑1 (MQ=255)

                                                               tGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATg                                 >  2:424879/1‑98 (MQ=255)

                                                               tGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGa                               <  1:52704/100‑1 (MQ=255)

                                                                gCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGAc                              >  2:942013/1‑100 (MQ=255)

                                                                gCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGAc                              >  1:2127254/1‑100 (MQ=255)

                                                                  cAGAGGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACgg                            >  1:2159492/1‑100 (MQ=255)

                                                                     agGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGTACGGTGc                         <  1:1350096/100‑1 (MQ=255)

                                                                     agGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGc                         <  1:771939/100‑1 (MQ=255)

                                                                     agGCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGc                         <  1:2177133/100‑1 (MQ=255)

                                                                       gCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTg                       <  1:2321159/100‑1 (MQ=255)

                                                                       gCGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTg                       <  1:2328322/100‑1 (MQ=255)

                                                                        cGGTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGa                      <  2:1306091/100‑1 (MQ=255)

                                                                          ggtCGGCGCGTCGGGTTCGAACCAGCCCGTGGCGGGAATGCCGGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAg                    >  1:1732851/3‑100 (MQ=255)

                                                                          gTTCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAg                    <  2:2185249/100‑1 (MQ=255)

                                                                           ttCGGCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAgg                   <  1:919849/100‑1 (MQ=255)

                                                                              ggCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGGGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCgg                >  2:1690429/1‑100 (MQ=255)

                                                                              ggCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCgg                <  2:732679/100‑1 (MQ=255)

                                                                              ggCGCGTCGTGTTCGAACCAGCCCGTGGCGGGAATGCAAGGTGACGCGACCGGCAGCAAGCCATGGCGGACCATCGGGCAATGGGCGATGCTGAAGGCgg                >  1:1492207/1‑100 (MQ=255)

                                                                              ggCGCGTCGTGTTCGAACCAGCCCGGGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCgg                >  1:732288/1‑100 (MQ=255)

                                                                                cgcgTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGc              <  1:2132355/100‑1 (MQ=255)

                                                                                 gcgTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCa             <  2:2208884/100‑1 (MQ=255)

                                                                                 gcgTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCa             <  1:63762/100‑1 (MQ=255)

                                                                                 gcgTCGTGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTGATGGACGGTGCTGAAGGCGGGCa             >  2:1019272/1‑100 (MQ=255)

                                                                                  cgtcgtGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCAt            <  2:1916288/100‑1 (MQ=255)

                                                                                    tcgtGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATgg          <  1:599275/100‑1 (MQ=255)

                                                                                    tcgtGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATgg          <  2:566037/100‑1 (MQ=255)

                                                                                    tcgtGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATgg          <  1:2094236/100‑1 (MQ=255)

                                                                                    tcgtGTTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCAGCAGGTTATGGACGGTGCTGAAGGCGGGCATgg          <  2:566266/100‑1 (MQ=255)

                                                                                     cgtGTTCGAACCAGCCCGTGGCGGAAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCGATCAGGTCATGGTCGGTGCTGAAGGGGGGCa             >  2:263124/1‑96 (MQ=255)

                                                                                      gtgtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtg        <  1:2207746/100‑1 (MQ=255)

                                                                                      gtgtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtg        <  1:2499723/100‑1 (MQ=255)

                                                                                      gtgtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGgtg        <  1:2504533/100‑1 (MQ=255)

                                                                                        gtTCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGtgtg      >  1:1290004/1‑100 (MQ=255)

                                                                                         ttCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGc     >  2:1987679/1‑100 (MQ=255)

                                                                                         ttCGAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGc     >  2:1706355/1‑100 (MQ=255)

                                                                                            gAACCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGcccc  >  1:1014536/1‑100 (MQ=255)

                                                                                              aCCAGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTGTGGACGGTGCTGAAGGCGGGCATGGTGTGcccc  >  2:2301412/1‑98 (MQ=255)

TCCAGCAGGGTGAGCGGATGGCAGCGTCCACCGCCAAAAGGAAAGTGGCCCTTAAAATCCATCTGCCAGAGGCGGTTCGGCGCGTCGTGTTCGAACCGGCCCGTGGCGGGAATGCCCGGTGAAGCGCCCGGCAGCAGGCCATGGCGGGCCATCAGGTTATGGACGGTGCTGAAGGCGGGCATGGTGTGCCCC  >  NC_000913/281484‑281675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`