Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,853,909	A→G	100%	intergenic (+267/‑13)	tisB → / → emrD	toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,853,909	0	A	G	100.0%	324.3 / NA	94	intergenic (+267/‑13)	tisB/emrD	toxic membrane persister formation peptide, LexA‑regulated/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (55/39);  total (55/39)

CTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATAT  >  NC_000913/3853815‑3854004                                                                                               |                                                                                                cTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATc                                                                                            >  1:2545673/1‑100 (MQ=255)   ggCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCg                                                                                          >  2:218313/1‑100 (MQ=255)     cGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCg                                                                                          >  2:1554738/1‑98 (MQ=255)       tataTCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGAt                                                                                      <  1:1457616/100‑1 (MQ=255)        atatCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATa                                                                                     <  2:1984691/100‑1 (MQ=255)          atCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAAt                                                                                   <  2:741092/100‑1 (MQ=255)           tCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATGAAGGCATCCGTGATAATg                                                                                  <  2:2116305/100‑1 (MQ=255)           tCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATg                                                                                  >  1:990421/1‑100 (MQ=255)           tCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATg                                                                                  >  1:1213978/1‑100 (MQ=255)           tCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATg                                                                                  >  2:1299784/1‑100 (MQ=255)            cTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGa                                                                                 <  1:620222/100‑1 (MQ=255)              ggCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGaaa                                                                               >  1:1745997/1‑100 (MQ=255)               gCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGaaaa                                                                              <  1:1762069/100‑1 (MQ=255)                cTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAg                                                                             >  1:2364195/1‑100 (MQ=255)                cTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAg                                                                             >  1:986408/1‑100 (MQ=255)                 tAATATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGAAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAgg                                                                            <  1:432349/100‑1 (MQ=255)                  aaCATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGc                                                                           <  1:1903053/100‑1 (MQ=255)                    cATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCaa                                                                         >  1:1579821/1‑100 (MQ=255)                     aTTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCaaa                                                                        <  2:1790333/100‑1 (MQ=255)                     aTTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCaaa                                                                        <  2:1305032/100‑1 (MQ=255)                      ttCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAg                                                                       >  1:54271/1‑100 (MQ=255)                       tcatcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTCTTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGa                                                                      <  2:1572829/100‑1 (MQ=255)                        catcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGaa                                                                     >  1:1576238/1‑100 (MQ=255)                        catcaATGGGATAGATTCCGCTCCCGCTTTTATGTGAATGCGTAGTGACTTCTTCTAATTATTTTTATAAAGGCATCCGTGATATTGAAAAGGcgacaaa                                                                     >  2:1261421/1‑94 (MQ=255)                         atcaATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGaaa                                                                    >  2:2419034/1‑100 (MQ=255)                           caATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACg                                                                  <  1:697222/100‑1 (MQ=255)                           caATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACg                                                                  <  2:1576227/100‑1 (MQ=255)                           caATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACg                                                                  >  1:2338468/1‑100 (MQ=255)                            aaTGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGCAACg                                                                  >  2:501885/1‑99 (MQ=255)                             aTGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTc                                                                <  1:1132212/100‑1 (MQ=255)                               gtgATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCGTCCGTGATAATGAAAAGGCAAAGAAACGTCaa                                                              >  1:552437/1‑100 (MQ=255)                               gtgATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCaa                                                              >  2:528966/1‑100 (MQ=255)                               gtgATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCaa                                                              <  1:2329780/100‑1 (MQ=255)                                tgATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAAt                                                             <  1:1039086/100‑1 (MQ=255)                                 gATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAAtt                                                            >  1:332335/1‑100 (MQ=255)                                 gATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACGTATTCTAATTATTTTTATAAAGGCATCCGTGATAAAGAAAAGGCAAAGAAACGGCAAtt                                                            >  1:757401/1‑100 (MQ=255)                                  aTAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAAttt                                                           <  2:278467/100‑1 (MQ=255)                                   tAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTg                                                          >  2:1249654/1‑100 (MQ=255)                                      aTTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTa                                                       >  1:326760/1‑100 (MQ=255)                                       ttCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTAt                                                      >  1:1482469/1‑100 (MQ=255)                                        tCCTCTCCCGCATTTAAGGGAATGCGTAGTGACTTATTTTAATTATTTTTATAAAGGCATCCGGGATAATGAAAAGGCAAAGAAACGTCAATTTGGTAtt                                                     >  1:633530/1‑100 (MQ=255)                                         ccTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTg                                                    >  1:2017136/1‑100 (MQ=255)                                          ctctCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGa                                                   >  1:1008254/1‑100 (MQ=255)                                          ctctCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGa                                                   >  1:491280/1‑100 (MQ=255)                                              cccGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGtt                                               <  1:2261089/100‑1 (MQ=255)                                              cccGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGtt                                               >  1:1778728/1‑100 (MQ=255)                                               ccGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTg                                              >  2:359005/1‑100 (MQ=255)                                                cGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTgg                                             >  2:2321691/1‑100 (MQ=255)                                                cGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTgg                                             >  1:2208017/1‑100 (MQ=255)                                                cGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTg                                              >  2:3589/1‑99 (MQ=255)                                                 gCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGt                                            >  2:1296892/1‑100 (MQ=255)                                                   aTTTATGGGAATGCGTTTTGACTTATTTTAATTATTTTTATATTGTTATTCTTTATAATTAAAAGGGTATGAAACGTTAATTTGTTATTGATg                                                 <  2:480727/93‑1 (MQ=255)                                                   aTTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTAt                                          <  1:1245241/100‑1 (MQ=255)                                                       aTGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGGTAATGAAAAGGCAAAGAAATGACAATTTGTTAATTATGATAGTAt                                          >  2:2439250/1‑96 (MQ=255)                                                           gAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTg                                  <  1:1723339/100‑1 (MQ=255)                                                           gAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTg                                  <  1:1724155/100‑1 (MQ=255)                                                           gAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTg                                  <  2:326756/100‑1 (MQ=255)                                                           gAATGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTg                                  <  1:1336763/100‑1 (MQ=255)                                                            aaTGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTgg                                 <  2:162551/100‑1 (MQ=255)                                                             aTGCGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGc                                <  1:262436/100‑1 (MQ=255)                                                                cGTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGt                             <  1:808611/100‑1 (MQ=255)                                                                 gTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTc                            <  1:1569958/100‑1 (MQ=255)                                                                 gTAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTc                            <  2:2295692/100‑1 (MQ=255)                                                                  tAGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAAATTGTTATTGATGTTGGTATTACTCGTGGCCGTCg                           >  1:150659/1‑100 (MQ=255)                                                                   aGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCgg                          <  2:413891/100‑1 (MQ=255)                                                                   aGTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCgg                          <  2:2118967/100‑1 (MQ=255)                                                                    gTGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGt                         >  2:2316810/1‑100 (MQ=255)                                                                     tGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTc                        <  2:1219016/100‑1 (MQ=255)                                                                     tGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTc                        <  2:2336031/100‑1 (MQ=255)                                                                     tGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTc                        <  2:1282320/100‑1 (MQ=255)                                                                     tGACTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATCGATGTTGGTATTACTCGTGGCCGTCGGTc                        >  1:2126639/1‑100 (MQ=255)                                                                        cTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCTGTCAGa                     >  2:404997/1‑100 (MQ=255)                                                                        cTTATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGa                     >  1:1142535/1‑100 (MQ=255)                                                                         ttATTCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGAt                    <  1:58382/100‑1 (MQ=255)                                                                             tCTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGCATTACTCTTGGTCGTCGGTCAGATGgc                 >  1:1336737/1‑99 (MQ=255)                                                                              cTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAATGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgcgc               >  2:313807/1‑100 (MQ=255)                                                                              cTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGgcgc               >  1:1925594/1‑100 (MQ=255)                                                                              cTAATTATTTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCATCGGTCAGATGgcgc               >  1:1425633/1‑100 (MQ=255)                                                                                 attattTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCaaa            >  1:1698869/1‑100 (MQ=255)                                                                                  ttattTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAAc           <  1:1504141/100‑1 (MQ=255)                                                                                   tattTTTATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAAcc          >  1:183047/1‑100 (MQ=255)                                                                                     tttttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCAt        >  2:2390916/1‑100 (MQ=255)                                                                                     tttttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCAt        >  2:1020223/1‑100 (MQ=255)                                                                                     tttttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCAt        >  1:2448949/1‑100 (MQ=255)                                                                                     tttttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATGTGTGATTGATGTGGGTATAACTGGTGGCCGTAGGTCAGATGGCGCAAAcc          >  2:2553766/1‑98 (MQ=255)                                                                                     tttttAGAAAGGCATTCTTGATAATGAAAAGGCATTGAAATTTAGATTTGTTATTGATGGTGGTATTACTCGTGGCTGTCGGTCAGATGGCGCaa             >  2:477806/1‑95 (MQ=255)                                                                                       tttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCAttt      >  2:1295886/1‑100 (MQ=255)                                                                                        ttATAAAGGCATTCGTGATAATGAAAAGGCAAAGAAACGTCAATTGGTTATTGATTTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTta     <  2:743376/100‑1 (MQ=255)                                                                                        ttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTta     <  1:1562434/100‑1 (MQ=255)                                                                                        ttATAAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTta     <  1:1562453/100‑1 (MQ=255)                                                                                         tataAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTtat    >  1:1433516/1‑100 (MQ=255)                                                                                         tataAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTtat    >  1:1049145/1‑100 (MQ=255)                                                                                           taAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTatat  >  2:2350501/1‑100 (MQ=255)                                                                                           taAAGGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTatat  >  1:2083240/1‑100 (MQ=255)                                                                                               |                                                                                                CTGGCGTATATCTGGCTAACATTCATCAATGTGATAGATTCCTCTCCCGCATTTATGGGAATGCGTAGTGACTTATTCTAATTATTTTTATAAAAGCATCCGTGATAATGAAAAGGCAAAGAAACGTCAATTTGTTATTGATGTTGGTATTACTCGTGGCCGTCGGTCAGATGGCGCAAACCATTTATAT  >  NC_000913/3853815‑3854004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑