Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,925 A→T 15.5% intergenic (‑112/+87) ppiC ← / ← yifN peptidyl‑prolyl cis‑trans isomerase C (rotamase C)/PemK toxin family pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,9250AT15.5% 258.2 / 30.0 101intergenic (‑112/+87)ppiC/yifNpeptidyl‑prolyl cis‑trans isomerase C (rotamase C)/PemK toxin family pseudogene
Reads supporting (aligned to +/- strand):  ref base A (44/41);  new base T (5/11);  total (49/52)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

GTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGC  >  NC_000913/3959827‑3960024
                                                                                                  |                                                                                                   
gTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGaaaa                                                                                                    >  1:1611244/1‑100 (MQ=255)
    ataatTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCg                                                                                                >  2:1163338/1‑100 (MQ=255)
     taatTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCgg                                                                                               >  2:2513046/1‑100 (MQ=255)
      aatTGTCAGTCGGCGATTTTAGAATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACTTATATGGCAAAAGCCCGGAAAATCCggg                                                                                              <  2:826927/100‑1 (MQ=255)
      aatTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTTGCTGGTACATATATGGCAAAAGCCCGGAAAATCCggg                                                                                              <  2:1368467/100‑1 (MQ=255)
         tGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCtt                                                                                           <  1:2510854/100‑1 (MQ=255)
         tGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCt                                                                                            >  2:1529817/1‑99 (MQ=255)
          gTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGCGTGGCTGGTACAAATATGGCAAAAGCCCGGCAAATCCGGGCTTc                                                                                          >  2:1258837/1‑100 (MQ=255)
           tCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCtttt                                                                                         <  2:1679186/100‑3 (MQ=255)
            cAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCttttg                                                                                        <  2:1223161/100‑4 (MQ=255)
            cAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCttttg                                                                                        <  1:722620/100‑4 (MQ=255)
            cAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCttttg                                                                                        <  1:2530172/100‑4 (MQ=255)
            cAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGc                                                                                        >  1:977032/1‑100 (MQ=255)
            cAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGc                                                                                        <  2:1749597/100‑1 (MQ=255)
             aGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCttttgc                                                                                       <  2:2046702/100‑5 (MQ=255)
             aGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCttttgc                                                                                       <  1:1291899/100‑5 (MQ=255)
             aGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCa                                                                                       >  2:2070711/1‑100 (MQ=255)
             aGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCa                                                                                       <  1:389482/100‑1 (MQ=255)
             aGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCa                                                                                       <  1:1822479/100‑1 (MQ=255)
             aGGCGGCGATTTTAGCATGATCCGCAGACGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGATTTTCCGGGCttttgc                                                                                       <  1:912725/100‑5 (MQ=255)
              ggcggcGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAg                                                                                      >  2:1783542/1‑100 (MQ=255)
                cggcgATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGt                                                                                    >  1:1396525/1‑100 (MQ=255)
                cggcgATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGt                                                                                    <  2:1343199/100‑1 (MQ=255)
                 ngcgATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTc                                                                                   >  1:1275619/2‑100 (MQ=255)
                 ggcgATTTTACCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTc                                                                                   >  2:1455416/1‑100 (MQ=255)
                   cgAGTTTAGCATGAGCCGTAGATTCTAACTTAAGTGGCAGGCACTGACTGGCTGGGTCATATATGGCAAAAGCCCGGATTTGCCGGGCttttgc                                                                                       <  2:2048871/94‑5 (MQ=255)
                    gATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCt                                                                                >  1:578025/1‑100 (MQ=255)
                         tAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAg                                                                            >  2:695659/1‑99 (MQ=255)
                          aGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAggg                                                                          <  1:2397378/100‑1 (MQ=255)
                          aGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAggg                                                                          <  1:2120886/100‑1 (MQ=255)
                          aGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAggg                                                                          <  1:1977026/100‑1 (MQ=255)
                           tcttGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAgggg                                                                         <  1:660439/97‑1 (MQ=255)
                           gCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAgggg                                                                         >  2:1828333/1‑100 (MQ=255)
                           gCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGAGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAgggg                                                                         >  1:1807345/1‑100 (MQ=255)
                             aTGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGga                                                                        <  2:609480/99‑1 (MQ=255)
                              tGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGgaga                                                                      >  2:660432/1‑100 (MQ=255)
                               gATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaa                                                                     >  2:819087/1‑100 (MQ=255)
                                aTCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGaaa                                                                    <  1:1478284/100‑1 (MQ=255)
                                  ccGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAAc                                                                  >  2:2292286/1‑100 (MQ=255)
                                    gCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACtt                                                                <  2:39906/100‑1 (MQ=255)
                                     cAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGTCTTCGCAGGTCCCTCAATGGGAGAAAACTTa                                                               <  1:1725432/100‑1 (MQ=255)
                                     cAGATGCTAACGTAATTGTCAGTCAGCGAGTGGCTGGTACATATATGGCAAAAGACCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTa                                                               <  1:2041350/100‑1 (MQ=255)
                                       gacgCTCACGTAATTGTCAGGCGTGGAGTGGCTGGTACATGTACGCCAAAAGGCCGGAATAACCGGGCTGCGGAGAGCCCTCAAGGCTAGAAAACTTat                                                              <  2:1983018/96‑2 (MQ=255)
                                         tGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGc                                                           <  2:1466305/100‑1 (MQ=255)
                                          gCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGcc                                                          <  2:2463950/100‑1 (MQ=255)
                                           cTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCct                                                         >  1:1154169/1‑100 (MQ=255)
                                            tAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCctc                                                        >  2:2236090/1‑100 (MQ=255)
                                             aaCGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCctct                                                       >  1:174509/1‑100 (MQ=255)
                                                gTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATg                                                    <  1:204068/100‑1 (MQ=255)
                                                 tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATgg                                                   <  2:1904776/100‑1 (MQ=255)
                                                   aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                                 <  1:1376645/100‑1 (MQ=255)
                                                   aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                                 <  1:459836/100‑1 (MQ=255)
                                                   aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAAAAAACTTAGTGCCTCTATggcc                                                 >  2:1694642/1‑98 (MQ=255)
                                                     atctagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTa                                               <  2:1739333/96‑1 (MQ=255)
                                                      gtcagtcaGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                                <  2:862497/98‑1 (MQ=255)
                                                      gtcagtcaGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                              <  2:1173136/100‑1 (MQ=255)
                                                        cagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                                 <  2:1873651/95‑1 (MQ=255)
                                                        cagtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaa                                            <  1:2164239/100‑1 (MQ=255)
                                                         aatcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTaagat                                           >  1:1739345/3‑96 (MQ=255)
                                                          gtcagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAg                                          <  2:373020/100‑1 (MQ=255)
                                                            cagGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTc                                        >  2:1100748/1‑100 (MQ=255)
                                                             agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCa                                       <  1:611407/100‑1 (MQ=255)
                                                             agGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCa                                       <  1:1448003/100‑1 (MQ=255)
                                                                gAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTa                                    >  2:1653568/1‑100 (MQ=255)
                                                                  gTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATc                                  >  2:1905886/1‑100 (MQ=255)
                                                                    ngCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgg                                >  2:117608/2‑100 (MQ=255)
                                                                    ggCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgg                                >  1:2551229/1‑100 (MQ=255)
                                                                    ggCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgg                                <  1:651257/100‑1 (MQ=255)
                                                                     gCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                               <  1:1905900/100‑1 (MQ=255)
                                                                      cTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                              <  1:877490/100‑1 (MQ=255)
                                                                       tGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                             >  2:393642/1‑100 (MQ=255)
                                                                        ggTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAt                            >  2:711811/1‑100 (MQ=255)
                                                                          tACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATgg                          >  2:87259/1‑100 (MQ=255)
                                                                              tatatGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttat                      >  1:324194/1‑100 (MQ=255)
                                                                               atatGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt                     >  1:319459/1‑100 (MQ=255)
                                                                                tatGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattt                    >  1:895929/1‑100 (MQ=255)
                                                                                gacctgcgaaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattt                    >  2:100503/9‑100 (MQ=38)
                                                                                 atGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttt                   >  1:2252935/1‑100 (MQ=255)
                                                                                  tGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta                  >  1:1703681/1‑100 (MQ=255)
                                                                                   ggCAAAAGCCCGGAAAATCCGGGCTTCTCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATAGGCTACAAAGTCATTATCGGGGATGGttattttat                 <  2:2283273/100‑1 (MQ=255)
                                                                                    tgcgaaGCCCGGATTTTCCGGGCTTTGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttatt                >  2:1917906/5‑100 (MQ=38)
                                                                                     cAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta                  >  2:611271/1‑97 (MQ=255)
                                                                                     cAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg               <  1:2380406/100‑1 (MQ=255)
                                                                                     cAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg               >  2:654859/1‑100 (MQ=255)
                                                                                     cAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTg               >  1:431919/1‑100 (MQ=255)
                                                                                     cAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAAATTAGGGCCTCTATGGGATACAAAGTCATTATCGGGGATGGTTATTTTATTg               >  2:612248/1‑100 (MQ=255)
                                                                                      cgaaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAAATTAGGGCCTCTATGGGCTACAAAGACATTATCGGAGACGGTTATTTTATTg               >  2:2443684/3‑99 (MQ=255)
                                                                                       gaaGCCCGGATTTTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAATCATTAACGGGGATGGATCTTTTATTGt              >  2:2221345/2‑99 (MQ=255)
                                                                                       aaaGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTc             <  1:1302410/100‑1 (MQ=255)
                                                                                         aGCCCGGATTTTCAGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTCTGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCaa           >  2:948697/1‑100 (MQ=255)
                                                                                          gCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAAt          <  2:1904396/100‑1 (MQ=255)
                                                                                            ccGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAAttt        >  1:1202975/1‑100 (MQ=255)
                                                                                            ccGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAAttt        >  2:326944/1‑100 (MQ=255)
                                                                                            ccGGAAAATCCGGGCTTCGCAGGACCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGATATTTCATTGTCaa           >  2:881977/1‑97 (MQ=255)
                                                                                             cGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTa       <  1:193524/100‑1 (MQ=255)
                                                                                              ggAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGTGGATGGTTATTTTATTGTCAATTTAt      >  2:971327/1‑100 (MQ=255)
                                                                                              ggAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTAt      <  2:1600990/100‑1 (MQ=255)
                                                                                               gAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATg     >  2:672822/1‑100 (MQ=255)
                                                                                               gAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATg     <  2:654572/100‑1 (MQ=255)
                                                                                                  aaTCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGc  >  2:2449495/1‑100 (MQ=255)
                                                                                                  |                                                                                                   
GTGAATAATTGTCAGGCGGCGATTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAAGCCCGGAAAATCCGGGCTTCGCAGGTCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATTTATGAGC  >  NC_000913/3959827‑3960024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: