Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,051,259 G→T 15.2% intergenic (‑494/‑88) yihA ← / → yihI cell division GTP‑binding protein/activator of Der GTPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,051,2590GT15.2% 285.6 / 32.8 104intergenic (‑494/‑88)yihA/yihIcell division GTP‑binding protein/activator of Der GTPase
Reads supporting (aligned to +/- strand):  ref base G (52/35);  new base T (3/13);  total (56/48)
Fisher's exact test for biased strand distribution p-value = 5.06e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

CACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCAT  >  NC_000913/4051160‑4051356
                                                                                                   |                                                                                                 
cacGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACagag                                                                                                   >  1:2100713/1‑100 (MQ=255)
cacGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACagag                                                                                                   >  2:135280/1‑100 (MQ=255)
 acGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGt                                                                                                  >  1:1975367/1‑100 (MQ=255)
 acGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGt                                                                                                  >  1:1674110/1‑100 (MQ=255)
  cGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTa                                                                                                 >  2:138965/1‑100 (MQ=255)
  cGGATTGGTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACagag                                                                                                   <  2:1380444/98‑1 (MQ=255)
    gATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTaa                                                                                                <  2:435290/99‑1 (MQ=255)
    gATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAAt                                                                                               <  1:2189161/100‑1 (MQ=255)
     aTTGTGCCGGCTAAAGGAAAAACAGGGTGTCTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATc                                                                                              <  2:2542174/100‑1 (MQ=255)
        gTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGt                                                                                           >  1:2491979/1‑100 (MQ=255)
        gTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGt                                                                                           >  1:110764/1‑100 (MQ=255)
        attCCGGCTAAAGGAAAAACAGGGGGTGTTGGCGGCCTGCAAGGATGGTAAGACGCGTTACGGGTCATGAGTAAGGAAAAAAGGCGAAAGAATa                                                                                                 >  2:2155418/2‑94 (MQ=255)
           ccGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGc                                                                                        >  2:339454/1‑100 (MQ=255)
            cGGCTAAAGGAAAAGCAGGGGTTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATTAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGc                                                                                        <  2:846646/99‑1 (MQ=38)
            cGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGcc                                                                                       <  1:2031349/100‑1 (MQ=255)
            cGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGcc                                                                                       <  2:588198/100‑1 (MQ=255)
             ggCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGcc                                                                                       <  1:240925/99‑1 (MQ=255)
             ggCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCt                                                                                      >  1:1337939/1‑100 (MQ=255)
              gCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCtt                                                                                     <  1:1009951/100‑1 (MQ=255)
              gCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCtt                                                                                     <  1:1997293/100‑1 (MQ=255)
              gCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCtt                                                                                     <  1:2330473/100‑1 (MQ=255)
              gCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCt                                                                                      <  1:2466691/99‑1 (MQ=255)
               cTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCttt                                                                                    <  2:641439/100‑1 (MQ=255)
                tAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCtttt                                                                                   <  2:1145881/100‑1 (MQ=255)
                tAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCtttt                                                                                   >  1:1423631/1‑100 (MQ=255)
                  aaGGAAAAGCAAGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTGTGGGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCtgttt                                                                                  >  2:850661/1‑99 (MQ=38)
                  aaGGAAAAACAGGGTGTGTTGGCTGTCTGCAAGGATTGTAGGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCtttttt                                                                                 <  2:1273453/100‑1 (MQ=37)
                  aaGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCtttttt                                                                                 >  1:773634/1‑100 (MQ=255)
                    ggAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCt                                                                               >  1:568373/1‑100 (MQ=255)
                     gAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCtn                                                                              <  2:48458/100‑2 (MQ=255)
                        aagcAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGATTACTCTGTCGCCTTTTTTcctgac                                                                           <  2:1623769/97‑6 (MQ=25)
                             gggTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTtgcttgct                                                                      >  1:675010/1‑100 (MQ=255)
                              ggTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACATAGTAATCTGTCGCCTTTTTTCTTTgcttgctt                                                                     <  1:2426221/100‑1 (MQ=255)
                                  tgtTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGACAACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCt                                                                  >  2:2340727/1‑99 (MQ=255)
                                   gtTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGt                                                                <  2:194537/100‑1 (MQ=255)
                                    ttGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGtt                                                               <  1:556258/100‑1 (MQ=255)
                                    ttGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGtt                                                               <  1:581559/100‑1 (MQ=255)
                                     tGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTa                                                              <  1:1107192/100‑1 (MQ=255)
                                     tGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTa                                                              <  2:1195544/100‑1 (MQ=255)
                                      ggcggcCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAg                                                             >  2:67030/1‑100 (MQ=255)
                                       gcggcCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGa                                                            >  1:550261/1‑100 (MQ=255)
                                              gCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgcc                                                     <  1:488070/100‑1 (MQ=255)
                                              gCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgcc                                                     >  1:872330/1‑100 (MQ=255)
                                               cAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTccgccg                                                    >  1:1268398/1‑100 (MQ=255)
                                                aaGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCcgccgc                                                   >  1:714722/1‑100 (MQ=255)
                                                  ggATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCaa                                                 >  2:2493108/1‑100 (MQ=255)
                                                  ggATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCaa                                                 >  2:1203795/1‑100 (MQ=255)
                                                   atctGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCaaa                                                <  1:127743/97‑1 (MQ=255)
                                                    aTTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAAt                                               >  2:1438358/1‑100 (MQ=255)
                                                       gTAAGACCCGTTAAGTGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCta                                            >  2:754737/1‑100 (MQ=255)
                                                       gTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCt                                             >  1:1526186/1‑99 (MQ=255)
                                                           gACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACt                                        <  2:302678/100‑1 (MQ=255)
                                                            aCCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTg                                       <  1:942155/100‑1 (MQ=255)
                                                             cccGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGa                                      >  1:857844/1‑100 (MQ=255)
                                                             cccGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGa                                      >  1:1773328/1‑100 (MQ=255)
                                                             cccGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGa                                      >  1:728335/1‑100 (MQ=255)
                                                             cacGTTAAGGGTTATGAGTCAGGAAACAAGGCGACAGAGTATTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTg                                       <  2:672237/97‑1 (MQ=255)
                                                              ccGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGaa                                     <  1:1698608/100‑1 (MQ=255)
                                                                   aaGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCATTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATaaaa                                >  2:2265052/1‑100 (MQ=255)
                                                                    aGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAAc                               >  2:67735/1‑100 (MQ=255)
                                                                    aGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAAc                               <  2:1023480/100‑1 (MQ=255)
                                                                    aGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAAc                               >  1:305439/1‑100 (MQ=255)
                                                                    aGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAAc                               <  1:1006401/100‑1 (MQ=255)
                                                                     ggtttATGAGTCATGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAAc                               <  2:723014/96‑1 (MQ=255)
                                                                     gggTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAAc                               <  1:380790/99‑1 (MQ=255)
                                                                      ggTTATGAGTCAGGAAAAAAGGCGACAGATTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACgg                             <  2:305435/100‑1 (MQ=255)
                                                                      ggTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCATTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACgg                             >  2:652785/1‑100 (MQ=255)
                                                                       gTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGGATAAAACGGc                            >  2:1690085/1‑100 (MQ=255)
                                                                          aTGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTaa                         >  1:1065886/1‑100 (MQ=255)
                                                                           tGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTTTACTGAATAAAACGGCTaaa                        <  2:1561350/100‑1 (MQ=255)
                                                                           tGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTaaa                        >  2:2497107/1‑100 (MQ=255)
                                                                            gAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAGTCTATACTGAATAAAACGGCTaaa                        >  1:829806/1‑99 (MQ=255)
                                                                             aGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAg                      >  2:2520864/1‑100 (MQ=255)
                                                                              gTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTTAATAAAACGGCTaaaaaa                     >  2:1775367/1‑98 (MQ=255)
                                                                               tCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGAc                    <  2:1657030/100‑1 (MQ=255)
                                                                                   gAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAAc                >  1:1379492/1‑100 (MQ=255)
                                                                                       aaaGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCAtt            >  2:2366175/1‑100 (MQ=255)
                                                                                         aGGCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTAt          >  2:1473344/1‑100 (MQ=255)
                                                                                         aGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTAt          >  1:1109046/1‑100 (MQ=255)
                                                                                         aGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTAt          <  2:911990/100‑1 (MQ=255)
                                                                                          ggCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATg         >  1:767838/1‑100 (MQ=255)
                                                                                          ggCGACAGATTACTCTGTCGCCTTTTTTCTTTGCTTGCTTTCTATTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATg         >  2:2002371/1‑100 (MQ=39)
                                                                                          ggCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAACTCTATACTGAATAAAACGGCTAAAAGACGAACCATTATg         >  2:2318671/1‑100 (MQ=255)
                                                                                          ggCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATg         <  1:453878/100‑1 (MQ=255)
                                                                                          ggCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATg         <  2:639735/100‑1 (MQ=255)
                                                                                           gCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGa        <  1:67736/100‑1 (MQ=255)
                                                                                            cGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGaa       >  1:765677/1‑100 (MQ=255)
                                                                                            cGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGaa       >  1:1247590/1‑100 (MQ=255)
                                                                                            cGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGaa       <  1:185493/100‑1 (MQ=255)
                                                                                             gACAGAGTAATCTGTCGCCTTTTTTTTTTGCTTTCTTTCTGTTAGATTCCGCCGCAAATCTATACTTAATAAAACGGCTAAAAGACGAACCATTATGaaa      >  2:1236512/1‑100 (MQ=255)
                                                                                             gACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGaaa      >  1:2284882/1‑100 (MQ=255)
                                                                                             gACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGaaa      >  1:1872117/1‑100 (MQ=255)
                                                                                              aCAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAAc     <  2:1398816/100‑1 (MQ=255)
                                                                                              aCAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAAc     <  2:2415197/100‑1 (MQ=255)
                                                                                              aCAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAAc     <  2:265867/100‑1 (MQ=255)
                                                                                              aCAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGCTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAAc     <  1:2340741/100‑1 (MQ=255)
                                                                                              aCAGAGGAATCTGTCGCCGTTTTTCTTTACTTGCTTTCTGTTAGATTCCGTCGCAAATCTATACTGACTAAACCCGATAAAAGACGAACCATTATGAAAc     <  2:2512239/100‑1 (MQ=255)
                                                                                               cAGAGTAATCTGTCGCCTTTTTTTTTTTTTTTTTTTCTATAAGATTCCGCCGCAAATCTATACTGAATAAAACGTCTAAAAGACGCACCATTATGaaa      >  2:402138/1‑98 (MQ=255)
                                                                                                 gagTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTCGATTCCGCAGCAAATCTATACTTAACAAAAAGGCTAACAGACGAACCATTCTGAAAc     >  2:1671437/1‑97 (MQ=255)
                                                                                                 gagTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACcat  >  1:851632/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
CACGGATTGTTCCGGCTAAAGGAAAAACAGGGTGTGTTGGCGGCCTGCAAGGATTGTAAGACCCGTTAAGGGTTATGAGTCAGGAAAAAAGGCGACAGAGTAATCTGTCGCCTTTTTTCTTTGCTTGCTTTCTGTTAGATTCCGCCGCAAATCTATACTGAATAAAACGGCTAAAAGACGAACCATTATGAAACCAT  >  NC_000913/4051160‑4051356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: