Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	11,127	C→G	17.6%	S77T (AGT→ACT)	yaaW ←	UPF0174 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	11,127	0	C	G	17.6%	230.9 / 45.0	91	S77T (AGT→ACT)	yaaW	UPF0174 family protein
Reads supporting (aligned to +/- strand):  ref base C (38/37);  new base G (5/11);  total (43/48)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.89e-01

GTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCA  >  NC_000913/11028‑11226                                                                                                    |                                                                                                    ggaGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATAc                                                                                                     <  2:1555226/98‑1 (MQ=255) gTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATAc                                                                                                     <  2:1217759/100‑1 (MQ=255) gTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATAc                                                                                                     <  2:408893/100‑1 (MQ=255)    gACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTAt                                                                                                  <  1:1908754/100‑1 (MQ=255)    gACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTAt                                                                                                  >  2:436807/1‑100 (MQ=255)      cATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAACCATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTG‑TGGCGATAGTATCGc                                                                                               <  2:570482/100‑1 (MQ=255)      cATCTCTTTGTCGGCTCTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTAACGTGTACGCGCAGTTTGCTGGCGCTACTATCg                                                                                                <  2:1493900/100‑1 (MQ=255)        tctcTTTTTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATTGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTa                                                                                                   >  2:1300996/1‑95 (MQ=255)        tctcTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGcc                                                                                              <  2:866940/100‑1 (MQ=255)          tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGccac                                                                                            >  1:1973043/1‑100 (MQ=255)          tctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAACTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGccac                                                                                            <  1:790692/100‑1 (MQ=255)           ctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATAGTATCGccaac                                                                                           <  1:2554111/100‑3 (MQ=255)           ctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATAGTATCGccaac                                                                                           <  1:2108000/100‑3 (MQ=255)           ctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATAGTATCGccaac                                                                                           <  1:21076/100‑3 (MQ=255)           ctTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGccacc                                                                                           <  2:522030/100‑1 (MQ=255)            tttGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATAGTATCGccaaca                                                                                          <  1:985180/100‑1 (MQ=255)            tttGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATAGTATCGccaaca                                                                                          <  1:2302398/100‑1 (MQ=255)               gTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATAGTATCGCCAACaaac                                                                                       <  1:646484/100‑2 (MQ=255)               gTCGGCTTTCAGCTTCAATCGCTTTGAAACATCCAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATGGCCACCaaaa                                                                                       >  2:1681111/1‑100 (MQ=255)                 cGGCTTTCAGCTTCAATCGCTTTGTAACATCTAGCATAATGGCTCGAGACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATAGTATCGCCAACAAACTg                                                                                     <  2:988788/100‑1 (MQ=37)                 cGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATg                                                                                     >  1:1563018/1‑100 (MQ=255)                  ggCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGc                                                                                    >  2:2014982/1‑100 (MQ=255)                  ggCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGc                                                                                    >  2:1955376/1‑100 (MQ=255)                  ggCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGc                                                                                    <  1:482779/100‑1 (MQ=255)                   gCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGGTGGCGATAGTATCGCCAACAAACTGcg                                                                                   <  2:762524/100‑2 (MQ=38)                   gCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGGTGGCGATAGTATCGCCAACAAACTGcg                                                                                   <  2:762494/100‑2 (MQ=38)                    cTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTg                                                                                  >  1:420021/1‑100 (MQ=255)                    atctCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGGTGGCGATAGTATCGCCAACAAACTGcgc                                                                                  <  1:1530143/97‑3 (MQ=37)                        cAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGGTACAATGTACCGTGTCCGCGGAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAAt                                                                              <  2:775530/100‑1 (MQ=255)                        cAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAAt                                                                              >  2:267870/1‑100 (MQ=255)                        cAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGGTGGCGATAGTATCGCCAACAAACTGcgcagat                                                                              >  2:1530132/1‑94 (MQ=37)                         aGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAAtt                                                                             >  1:1629389/1‑100 (MQ=255)                          gCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACACTTTCCCGTATCCGCGCAGTTTGTACGCGATACTATCaccacccacctccg                                                                                   >  2:1258112/1‑85 (MQ=255)                             tCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTcc                                                                         <  1:247452/100‑1 (MQ=255)                              cAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCg                                                                        <  1:375107/100‑1 (MQ=255)                               aaTCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCgg                                                                       >  2:924696/1‑100 (MQ=255)                                aTCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTAGCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGc                                                                      >  2:1398600/1‑100 (MQ=255)                                    cTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATc                                                                  <  2:2544665/100‑1 (MQ=255)                                     tttGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCa                                                                 <  1:2249644/100‑1 (MQ=255)                                       tGAAACATCGAGCAAAATAACCCGATACAATTTACCGTATCCGCGCAATTTATTCACGATACTATCGCCACCAAAATGCTGTAATTCTCCAGCAATCa                                                                 >  2:512433/1‑98 (MQ=255)                                         aaaCATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTg                                                             <  2:307313/100‑1 (MQ=255)                                            cATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCa                                                          >  2:1185686/1‑100 (MQ=255)                                             aTCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAg                                                         <  2:2015133/100‑1 (MQ=255)                                              tCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGt                                                        >  1:431578/1‑100 (MQ=255)                                              tCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGt                                                        <  1:1873801/100‑1 (MQ=255)                                                gAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTg                                                      >  1:1319164/1‑100 (MQ=255)                                                  gCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTgcg                                                    <  1:559136/100‑1 (MQ=255)                                                  gCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTgcg                                                    >  2:688291/1‑100 (MQ=255)                                                    aaaaTGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTgcggc                                                  >  1:1021181/1‑100 (MQ=255)                                                    aaaaTGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTgcggc                                                  <  2:396728/100‑1 (MQ=255)                                                     aaaTGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTgcggcg                                                 <  1:993515/100‑1 (MQ=255)                                                      aaTGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTAGCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTgcggcg                                                 <  2:939029/99‑1 (MQ=255)                                                        tGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATg                                              <  1:480533/100‑1 (MQ=255)                                                        tGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTAGCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATg                                              <  2:2084152/100‑1 (MQ=255)                                                          gCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGtt                                            >  1:911412/1‑100 (MQ=255)                                                          gCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGtt                                            >  1:106362/1‑100 (MQ=255)                                                           cccGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTg                                           <  1:1846229/100‑1 (MQ=255)                                                            ccGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGc                                          <  1:530487/100‑1 (MQ=255)                                                            ccGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGc                                          >  1:187198/1‑100 (MQ=255)                                                                 aCAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCggg                                     >  2:1670071/1‑100 (MQ=255)                                                                  cAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGa                                    <  1:1226653/100‑1 (MQ=255)                                                                   atcttACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGAt                                   <  1:868056/97‑1 (MQ=255)                                                                   aaTTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGAt                                   >  1:2110159/1‑100 (MQ=255)                                                                   aaTTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGAt                                   <  1:819032/100‑1 (MQ=255)                                                                   aaTTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGAt                                   <  1:847299/100‑1 (MQ=255)                                                                       tACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGAtagat                               >  2:868048/1‑96 (MQ=255)                                                                       tACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGccc                               <  1:1952663/100‑1 (MQ=255)                                                                       tACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGccc                               <  1:2436153/100‑1 (MQ=255)                                                                        aCCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCt                              <  2:158580/100‑1 (MQ=255)                                                                           gtgtCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTcc                           <  1:660531/100‑1 (MQ=255)                                                                           gtgtCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTcc                           >  1:1854433/1‑100 (MQ=255)                                                                               ccGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATc                        <  2:590717/99‑1 (MQ=255)                                                                               ccGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCg                       >  2:2480359/1‑100 (MQ=255)                                                                               ccGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCg                       >  2:987118/1‑100 (MQ=255)                                                                               ccGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCg                       >  2:1833834/1‑100 (MQ=255)                                                                                acagcattttGGTGGCGATAGTATCGCCAACAAACTGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGa                      >  2:722306/8‑100 (MQ=25)                                                                                acagcATTTTGGTGGCGATAGTATCGCCAACAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCg                       >  1:2237853/4‑99 (MQ=37)                                                                                 gcgcAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGAt                     >  2:1451612/1‑100 (MQ=255)                                                                                  cgcAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGAtt                    <  1:113430/100‑1 (MQ=255)                                                                                   gcATTTTGGTGGCGATAGTATCGCCAACAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGAtt                    >  2:373052/1‑99 (MQ=38)                                                                                   gcAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGAttt                   >  1:1269671/1‑100 (MQ=255)                                                                                    cAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTa                  <  1:790305/100‑1 (MQ=255)                                                                                          gttgGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGt            <  2:318549/100‑1 (MQ=255)                                                                                          ggtgGCGATAGTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGt            >  1:2549185/3‑100 (MQ=255)                                                                                           ttgGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGt            <  2:1732207/99‑1 (MQ=255)                                                                                            tgGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTc          >  2:2245509/1‑100 (MQ=255)                                                                                             ggCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCg         >  1:1061052/1‑100 (MQ=255)                                                                                             ggCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCg         >  1:1312109/1‑100 (MQ=255)                                                                                               cGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGtt       >  2:1414647/1‑100 (MQ=255)                                                                                                gATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTg      >  1:43730/1‑100 (MQ=255)                                                                                                 aTACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTgc     >  1:878291/1‑100 (MQ=255)                                                                                                 aTACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTgc     >  1:878314/1‑100 (MQ=255)                                                                                                  tACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTgcg    <  1:719676/100‑1 (MQ=255)                                                                                                  tACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTgcg    <  1:129653/100‑1 (MQ=255)                                                                                                    cTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCTGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCa  <  2:614541/100‑1 (MQ=255)                                                                                                    cTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCa  <  2:441792/100‑1 (MQ=255)                                                                                                    |                                                                                                    GTAGACATCTCTTTGTCGGCTTTCAGCTTCAATCGCTTTGAAACATCGAGCAAAATGGCCCGATACAATTTACCGTGTCCGCGCAGTTTGTTGGCGATACTATCGCCACCAAAATGCTGTAATTCTCCGGCAATCAGCTGCCAGTTGCGGCGATGTTGCTCGGGATGCCCTTCCATCGATTTAAACAGTTCGTTGCGCA  >  NC_000913/11028‑11226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑