Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,178,296 A→T 100% intergenic (+8/‑151) nusG → / → rplK transcription termination factor/50S ribosomal subunit protein L11

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,178,2960AT96.4% 322.2 / 0.4 102intergenic (+8/‑151)nusG/rplKtranscription termination factor/50S ribosomal subunit protein L11
Reads supporting (aligned to +/- strand):  ref base A (2/2);  new base T (67/30);  total (70/32)
Fisher's exact test for biased strand distribution p-value = 5.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

TACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGA  >  NC_000913/4178199‑4178388
                                                                                                 |                                                                                            
tACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGATGGACTTCAGCCAAGTTGAAAAAGCCTAACCCAGCGTTc                                                                                            >  2:2046674/1‑100 (MQ=255)
 aCGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCa                                                                                           >  2:294117/1‑100 (MQ=255)
 aCGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCa                                                                                           >  1:9200/1‑100 (MQ=255)
  cGAGAATTCTCGTCTGAAACTAACGGTTAGCAGCTTTGGTGATCCGAACCGGGTAGAGCTGGACTGAAACCAGACAAAAAAAGCCTTATCCAGCGATc                                                                                            >  2:804723/1‑98 (MQ=255)
  cGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCaa                                                                                          >  2:384530/1‑100 (MQ=255)
  cGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCaa                                                                                          >  1:1654944/1‑100 (MQ=255)
  cGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCaa                                                                                          >  1:1142699/1‑100 (MQ=255)
  cGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAACTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCaa                                                                                          >  1:43912/1‑100 (MQ=255)
       aaTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAg                                                                                     >  1:829017/1‑100 (MQ=255)
       aaTCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAg                                                                                     >  2:1934480/1‑100 (MQ=255)
         tctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcg                                                                                   <  1:1785788/100‑1 (MQ=255)
          ctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcgg                                                                                  >  1:1498512/1‑100 (MQ=255)
          ctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcgg                                                                                  >  2:571231/1‑100 (MQ=255)
          ctcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACACCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcgg                                                                                  >  2:564814/1‑100 (MQ=255)
           tcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcggc                                                                                 <  1:1157740/100‑1 (MQ=255)
              tCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAACAAGCGGCGAt                                                                              >  2:1719295/1‑100 (MQ=255)
               cTGAAAGTGTCTGTTTCTATCTTCTGTCGTGCGACCCCTGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAgcggcg                                                                                <  2:1404580/97‑1 (MQ=255)
               cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGAtt                                                                             <  2:1118061/100‑1 (MQ=255)
               cTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGAtt                                                                             <  2:1246249/100‑1 (MQ=255)
                tGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAATCCTAACCCAGCGTTCAATAAAGCGGCGAttt                                                                            <  1:1726649/100‑1 (MQ=255)
                  aaaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTaa                                                                          >  1:1205833/1‑100 (MQ=255)
                    aGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATc                                                                        >  1:387954/1‑100 (MQ=255)
                    aGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATAAAAAAAGCGGCGATTTAATc                                                                        <  2:2332581/100‑1 (MQ=255)
                     gtgtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCg                                                                       >  2:2085981/1‑100 (MQ=255)
                       gtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGtt                                                                     >  2:2510962/1‑100 (MQ=255)
                       gtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGtt                                                                     >  1:201964/1‑100 (MQ=255)
                       gtCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGtt                                                                     >  1:1825074/1‑100 (MQ=255)
                        tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTg                                                                    <  1:1995485/100‑1 (MQ=255)
                          tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGca                                                                  >  1:839187/1‑100 (MQ=255)
                          tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGca                                                                  >  2:1817936/1‑100 (MQ=255)
                          tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGca                                                                  >  1:76476/1‑100 (MQ=255)
                          tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGca                                                                  >  1:1280787/1‑100 (MQ=255)
                           gTTTCTATCTTCGGTCGTGCGACCCCGGGAGAGCTGGACTTCAGCCAGAGTGAACACGCCTAACCCGGCGGTCAACAAAGCGGCGATTTAATCGTTCcac                                                                 >  2:635891/1‑100 (MQ=255)
                            tttCTATCCTCGGTCGTGCGACCCCGGTAGAGCTGGATTTAAAAAAAAAAAAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGcaca                                                                <  2:1309013/100‑1 (MQ=255)
                             ttCTATCTTCGGTCGTGCGACCCCGCTAGAGCGGGACTTCACCCAAGTTGAAAAAGCCTAACCCAGCATTCAAAAAACCGGCGAATTAATCGTTGCACaa                                                               >  2:2435083/1‑100 (MQ=255)
                              tCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAg                                                              <  2:1162673/100‑1 (MQ=255)
                               cTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAgg                                                             >  1:898599/1‑100 (MQ=255)
                                 aTCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCg                                                           >  2:276743/1‑100 (MQ=255)
                                   cTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                                         >  2:1123785/1‑100 (MQ=255)
                                    ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTga                                                        <  2:118808/100‑1 (MQ=255)
                                     tCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                                                       <  2:1654932/100‑1 (MQ=255)
                                     tCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTgag                                                       >  2:143359/1‑100 (MQ=255)
                                       ggTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAt                                                     >  1:579146/1‑100 (MQ=255)
                                       ggTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAt                                                     >  1:2249811/1‑100 (MQ=255)
                                       ggTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAt                                                     >  2:1694084/1‑100 (MQ=255)
                                        gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAtt                                                    <  1:1483048/100‑1 (MQ=255)
                                           gTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGa                                                 >  1:2433140/1‑100 (MQ=255)
                                            tGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGaa                                                <  2:2126576/100‑1 (MQ=255)
                                              cGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATa                                              >  2:2198044/1‑100 (MQ=255)
                                               gACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATAc                                             >  2:2152575/1‑100 (MQ=255)
                                                 ccccGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACaa                                           >  1:1145244/1‑100 (MQ=255)
                                                 ccccGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACaa                                           >  1:264040/1‑100 (MQ=255)
                                                 ccccGGTAGAGCTGGACTTCAGCCAGGCTGAAACAGCCTAACCCAGCGTTCAAAAAAGCGGCGCTTTAATCGTTGCAAAAGGCATGAGATTGGAATACaa                                           >  2:1039584/1‑100 (MQ=255)
                                                  cccGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGTGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAt                                          <  1:2069413/100‑1 (MQ=255)
                                                   ccGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAtt                                         >  2:1033545/1‑100 (MQ=255)
                                                   ccGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAtt                                         >  1:2244472/1‑100 (MQ=255)
                                                   ccGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAt                                          <  2:1857534/99‑1 (MQ=255)
                                                    cGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAAttt                                        >  2:1696287/1‑100 (MQ=255)
                                                     ggTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTc                                       >  1:2530920/1‑100 (MQ=255)
                                                        agagCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcgcg                                    <  2:2230714/100‑1 (MQ=255)
                                                          agCTGGACTTCAGCCAGGTTGAAAAAGCCTATCCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGcc                                  <  1:823983/100‑1 (MQ=255)
                                                          agCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCgcgc                                   >  1:755954/1‑99 (MQ=255)
                                                            ntGGACTTCAGCCAGGTTGAAACAGCATAAACCAGCGTTCAAGAAAGCGGCGCTTTAATAACAGCACAAGTCGTGAGATTGGACTACAACTTCGCGCCt                                 >  2:2502810/2‑99 (MQ=255)
                                                            cTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTAGCGCCtt                                >  1:519487/1‑100 (MQ=255)
                                                                aCTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGt                            >  2:639930/1‑100 (MQ=255)
                                                                 cTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtt                           >  1:2450186/1‑100 (MQ=255)
                                                                 cTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtt                           >  1:230021/1‑100 (MQ=255)
                                                                  ttCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttt                          <  1:230331/100‑1 (MQ=255)
                                                                   tCAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtttt                         >  2:4634/1‑100 (MQ=255)
                                                                    cAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttttt                        >  1:1555595/1‑100 (MQ=255)
                                                                    cAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttttt                        <  2:2334509/100‑1 (MQ=255)
                                                                    cAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttttt                        >  1:1818880/1‑100 (MQ=255)
                                                                    cAGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttttt                        >  1:2367206/1‑100 (MQ=255)
                                                                     aGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGCGATTGGAATACAATTTCGCGCCTTTTGttttt                        >  2:1705820/1‑99 (MQ=255)
                                                                     aGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTa                       >  2:2489983/1‑100 (MQ=255)
                                                                     aGCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTa                       <  1:1836607/100‑1 (MQ=255)
                                                                      gCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAt                      <  1:40357/100‑1 (MQ=255)
                                                                      gCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAt                      >  2:18297/1‑100 (MQ=255)
                                                                      gCCAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAt                      >  1:2473223/1‑100 (MQ=255)
                                                                        cAGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATgg                    <  2:1135002/100‑1 (MQ=255)
                                                                         aGGTTGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATggg                   <  2:1448359/100‑1 (MQ=255)
                                                                           gTTGAAAAAGGCTAACCCAGCGTGCAAAAAAGCGGCGATGTAATCGTTGCACAAGGCGTGAGATGGGAATACAATTTCGCGCCTTGTGTTTTTATggg                   <  2:447000/98‑1 (MQ=255)
                                                                            ttGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCt                >  2:1186931/1‑100 (MQ=255)
                                                                             tGAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCtt               >  1:2121544/1‑100 (MQ=255)
                                                                              gAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg              >  1:5146/1‑100 (MQ=255)
                                                                              gAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg              >  1:740446/1‑100 (MQ=255)
                                                                              gAAAAAGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg              >  2:1171481/1‑100 (MQ=255)
                                                                               aaaaaGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGGTTTTATGGGCCTTGc             >  1:2517776/1‑100 (MQ=255)
                                                                                  aaGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCg          <  1:344910/100‑1 (MQ=255)
                                                                                  aaGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCg          <  1:854287/100‑1 (MQ=255)
                                                                                  aaGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGAACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCg          <  2:886321/100‑1 (MQ=255)
                                                                                   aGCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGt         <  2:75171/100‑1 (MQ=255)
                                                                                   aGCCTAACCCAACGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCg          <  2:437651/99‑1 (MQ=255)
                                                                                    gCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTa        <  1:1694096/100‑1 (MQ=255)
                                                                                    gCCTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTa        >  1:1020731/1‑100 (MQ=255)
                                                                                      cTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaa      >  2:1433389/1‑100 (MQ=255)
                                                                                      cTAACCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaa      >  1:1758450/1‑100 (MQ=255)
                                                                                        aaCCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAAc    >  1:1179114/1‑100 (MQ=255)
                                                                                         aCCCAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACg   >  1:1132340/1‑100 (MQ=255)
                                                                                          cccAGCGTTCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACg   <  2:1318019/99‑1 (MQ=255)
                                                                                                 |                                                                                            
TACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGA  >  NC_000913/4178199‑4178388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: