Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,238,726 | A→G | 100% | I365V (ATT→GTT) | yjbH → | DUF940 family extracellular polysaccharide protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,238,726 | 0 | A | G | 97.7% | 282.3 / ‑3.5 | 83 | I365V (ATT→GTT) | yjbH | DUF940 family extracellular polysaccharide protein |
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (42/39); total (42/41) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 |
GCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCATTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCACGAAA > NC_000913/4238631‑4238822 | gCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCTTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTAc < 2:2310399/100‑1 (MQ=255) cGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACg < 1:20567/100‑1 (MQ=255) gAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACgg < 1:2301942/100‑1 (MQ=255) gAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACgg < 2:1776663/100‑1 (MQ=255) gCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGaa > 1:595931/1‑100 (MQ=255) ggTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAAt > 1:1594831/1‑100 (MQ=255) gTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATc < 1:405735/100‑1 (MQ=255) gTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATc > 1:348849/1‑100 (MQ=255) tGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCg > 1:1231191/1‑100 (MQ=255) gAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGc > 1:934446/1‑100 (MQ=255) aaaTATCGTGATTCGCTCGAATGGATCATCCGTGCGAATCTGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCg < 2:632159/98‑1 (MQ=255) aaaTATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGcc < 1:2361016/100‑1 (MQ=255) aaaGATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGcc < 2:585791/100‑1 (MQ=255) aaTATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCt < 1:1181421/100‑1 (MQ=255) atatCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCtt < 2:597843/100‑1 (MQ=255) tatCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTa > 2:1165505/1‑100 (MQ=255) tatCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTa > 2:798372/1‑100 (MQ=255) atCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGCGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTaa < 2:1794886/100‑1 (MQ=255) gTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCATAACGATCCGCGTTACGGAAAATCGCCTTAACAt > 2:1027874/1‑100 (MQ=255) gTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACa > 2:1023716/1‑99 (MQ=255) gTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACAt > 2:2037709/1‑100 (MQ=255) gTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACAt > 2:1370305/1‑100 (MQ=255) gTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACAt > 2:719653/1‑100 (MQ=255) gTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCATTACGGAAAATCGCCTTAAc < 2:2198956/98‑1 (MQ=255) ttCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCg > 1:2250608/1‑100 (MQ=255) tCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGc < 2:340843/100‑1 (MQ=255) gcgcgAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAg > 1:1425018/1‑100 (MQ=255) cgcgAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAgg > 1:486589/1‑100 (MQ=255) cgcgAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAgg < 1:931078/100‑1 (MQ=255) gAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCCAAACGATCCGCGTTACTGAAAATCGCCTTAACATGCCGCAGGTga > 2:2293488/1‑100 (MQ=255) gAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTga > 2:1956536/1‑100 (MQ=255) aaGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCCCAGGTg > 2:2172469/1‑98 (MQ=255) aaGGGATCATCCGTGCGAATCGGATCGTGAAGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTgac < 2:543915/100‑1 (MQ=255) gagATCATCCGTGAGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTgacga > 1:1727960/3‑100 (MQ=255) ggAACATCCGTGCGAATCGGCTCGTGATGACCGACCTGCCGGATGGGATCGAGGCGATCCGTGTTACGGGAAATCGCCTTAACATGCCGCAGGTcacgac < 2:1461153/100‑1 (MQ=255) gATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGacgacg > 1:1729235/1‑100 (MQ=255) atcatcCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACgg < 1:2265246/100‑1 (MQ=255) tcatcCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCATAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGa < 1:1027882/100‑1 (MQ=255) tcatcCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGa < 2:809279/100‑1 (MQ=255) tcatcCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGa < 1:1023724/100‑1 (MQ=255) ccGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAAcc < 1:1470998/100‑1 (MQ=255) ccGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAAcc < 1:275079/100‑1 (MQ=255) ccGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCATTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAAcc < 1:832130/100‑1 (MQ=255) cGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCAGCAGGTGACGACGGAAAcc < 2:1013937/99‑1 (MQ=255) gTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGa < 2:2098939/100‑1 (MQ=255) gTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGa < 2:540787/100‑1 (MQ=255) gTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGa > 2:1200350/1‑100 (MQ=255) tGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGAt > 2:201368/1‑100 (MQ=255) tGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGAt < 1:1509648/100‑1 (MQ=255) gAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTc > 1:364348/1‑100 (MQ=255) aaTCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCg > 1:156480/1‑100 (MQ=255) aaTCGGATCGTGATGAACGATCTCCCGGATGGGATCAAAACGAGCCGCGTTACGGAAACTCGCCTTAACAGGCCGCAGGTGACGGGGGAAACCGGTg > 2:2365601/1‑97 (MQ=255) gATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGc > 2:1216147/1‑100 (MQ=255) aTCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGcc > 1:668309/1‑100 (MQ=255) aTCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGcc < 2:2250594/100‑1 (MQ=255) aTCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGcc < 2:1738015/100‑1 (MQ=255) aTCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGc > 2:753665/1‑99 (MQ=255) tgatgaACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGaa > 1:1936586/1‑100 (MQ=255) tgaACGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGaaaaa < 1:2203881/100‑1 (MQ=255) aCGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAatca > 1:1553375/1‑100 (MQ=255) aCGATCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAatca < 1:1216155/100‑1 (MQ=255) aTCTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCAtct > 1:891989/1‑100 (MQ=255) cTGCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCg > 2:1511267/1‑100 (MQ=255) cTGCCGGACGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCg > 2:1028792/1‑100 (MQ=255) gCCGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGcc > 1:2481645/1‑100 (MQ=255) ccGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACTGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCg < 2:1369782/100‑1 (MQ=255) ccGGATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCg < 1:1751168/100‑1 (MQ=255) ggATGGGATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGga > 2:1709584/1‑100 (MQ=255) gggATCAAAACGATCCGCGTTACGGAAAAGCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGc < 2:2375300/100‑1 (MQ=255) ggATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGcc > 2:1063133/1‑100 (MQ=255) gATCAAAACGCTCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCg < 2:1684662/100‑1 (MQ=255) gATCAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGCGAGCCg > 2:639655/1‑100 (MQ=255) cAAAACGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTg > 2:2411129/1‑100 (MQ=255) aaaaCGATCCGCGTTACAGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTgg < 1:2471507/100‑1 (MQ=255) aaaCGGTCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTggg < 2:141039/100‑1 (MQ=255) aaaCGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTggg > 2:2471617/1‑100 (MQ=255) aaaCGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTggg < 1:1851323/100‑1 (MQ=255) aaaCGATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTggg < 1:415413/100‑1 (MQ=255) gATCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCCGCCTGAAAAATCATCTCGCCGGCGAGCCGTTGGGCCa > 1:22444/1‑99 (MQ=255) aTCCGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCACg > 1:687543/1‑100 (MQ=255) ccGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCACGaa > 1:650002/1‑100 (MQ=255) ccGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCACGaa < 1:1199172/100‑1 (MQ=255) ccGCGTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCACGaa < 1:2493137/100‑1 (MQ=255) cgcgTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCACGaaa > 2:1199122/1‑100 (MQ=255) cgcgTTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCACGaaa > 2:2484374/1‑100 (MQ=255) | GCGAGCAGGTGAAATATCGTGATTCGCGCGAAGGGATCATCCGTGCGAATCGGATCGTGATGAACGATCTGCCGGATGGGATCAAAACGATCCGCATTACGGAAAATCGCCTTAACATGCCGCAGGTGACGACGGAAACCGATGTCGCCAGCCTGAAAAATCATCTCGCCGGAGAGCCGTTGGGCCACGAAA > NC_000913/4238631‑4238822 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |