Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,279,370 G→T 100% A298S (GCA→TCA)  ghxP → guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,279,3700GT97.0% 325.8 / 1.4 103A298S (GCA→TCA) ghxPguanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (48/51);  total (51/52)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-01

Only 100 of 103 total aligned reads displayed.

GATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCG  >  NC_000913/4279273‑4279465
                                                                                                 |                                                                                               
gatgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATca                                                                                               >  1:1817321/1‑100 (MQ=255)
 atgaCGGCAGTATTTGATGCCACCGGAACTATGCGTGCCATCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcac                                                                                              <  2:1801561/100‑1 (MQ=255)
 atgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcac                                                                                              >  2:274515/1‑100 (MQ=255)
 atgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcac                                                                                              <  2:1700448/100‑1 (MQ=255)
  tgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcact                                                                                             >  1:2471173/1‑100 (MQ=255)
  tgaCGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcact                                                                                             >  1:2266134/1‑100 (MQ=255)
  tgaCGGCAGTATTTGATGCCACCGGAACTAGCCGTGCCGTCGCCGGCCAGGCGAACGTGGTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcac                                                                                              <  2:1783608/99‑1 (MQ=38)
     cGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgac                                                                                          <  1:797081/100‑1 (MQ=255)
      ggCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgacc                                                                                         <  2:1360739/100‑1 (MQ=255)
      ggCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgacc                                                                                         <  2:1360749/100‑1 (MQ=255)
      ggCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgacc                                                                                         >  1:480625/1‑100 (MQ=255)
      ggCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAAcactgaac                                                                                         >  1:2284323/1‑98 (MQ=255)
         aGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccact                                                                                      <  2:1871632/100‑1 (MQ=255)
            aTTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccactgac                                                                                   >  1:1660796/1‑100 (MQ=255)
            aTTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccactgac                                                                                   >  2:1760162/1‑100 (MQ=255)
            aTTTGATGCCACCGGAACTATCCGTGCCGTAGCGGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcacggaccactga                                                                                    >  2:1687309/1‑99 (MQ=255)
             tttGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACt                                                                                  <  2:736277/100‑1 (MQ=255)
              ttGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGTTAAAGATGGGCAGCTCATCGACGGTGGGAAATCACTGACCACTGACTc                                                                                 <  2:1228678/100‑1 (MQ=255)
               tGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATcactgaccact                                                                                      >  2:227820/1‑94 (MQ=255)
               tGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTcc                                                                                >  1:29696/1‑100 (MQ=255)
               tGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTcc                                                                                >  2:1327284/1‑100 (MQ=255)
               tGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTcc                                                                                >  2:1854077/1‑100 (MQ=255)
               tGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTcc                                                                                <  1:732284/100‑1 (MQ=255)
                 aTGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCAt                                                                              <  2:2309450/100‑1 (MQ=255)
                  tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATg                                                                             <  1:1383939/100‑1 (MQ=255)
                  tGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATg                                                                             <  2:657379/100‑1 (MQ=255)
                   gCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGa                                                                            <  1:335141/100‑1 (MQ=255)
                   gCCACCGGAACTATCCGTGCAGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCAt                                                                              >  2:200393/1‑98 (MQ=255)
                    ccACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGag                                                                           <  2:381685/100‑1 (MQ=255)
                     cACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGagc                                                                          <  2:1529796/100‑1 (MQ=255)
                     cACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGagc                                                                          >  1:596426/1‑100 (MQ=255)
                     cACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGagc                                                                          <  1:1854091/100‑1 (MQ=255)
                      aCCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAACATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGagca                                                                         >  2:1322282/1‑100 (MQ=255)
                       ccGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGagcag                                                                        <  2:1488766/100‑1 (MQ=255)
                       ccGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGagcag                                                                        <  2:1632823/100‑1 (MQ=255)
                       ccGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGagcag                                                                        >  2:89047/1‑100 (MQ=255)
                        cGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGagcagc                                                                       >  1:2007264/1‑100 (MQ=255)
                         ggAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCg                                                                      >  1:2446933/1‑100 (MQ=255)
                         ggAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCg                                                                      <  2:1471940/100‑1 (MQ=255)
                              tATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAATATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTtc                                                                 <  2:2467502/100‑1 (MQ=255)
                               aTCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTtct                                                                >  1:2473708/1‑100 (MQ=255)
                                  cGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTg                                                             >  1:1919378/1‑100 (MQ=255)
                                   gTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTgg                                                            <  1:431842/100‑1 (MQ=255)
                                   gTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTgg                                                            >  1:1574098/1‑100 (MQ=255)
                                    tGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGc                                                           >  2:1741749/1‑100 (MQ=255)
                                    tGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGc                                                           <  2:2015067/100‑1 (MQ=255)
                                     gCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGcc                                                          <  2:316937/100‑1 (MQ=255)
                                     gCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGcc                                                          >  2:2508407/1‑100 (MQ=255)
                                     gCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTAACCACTGACTCCATGAGCAGCGTTTTCTCTGGc                                                           >  2:2111949/1‑99 (MQ=255)
                                           gCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCtggtgg                                                    >  2:2474911/1‑100 (MQ=255)
                                            ccGGCAAGGCGAGCCGGGTGGATAAAGATGGGCGGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTggtggg                                                   <  2:1503309/100‑1 (MQ=255)
                                               gCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGc                                                >  1:2434412/1‑100 (MQ=255)
                                               gCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGc                                                <  2:2113208/100‑1 (MQ=255)
                                                ccAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCg                                               <  1:2404215/100‑1 (MQ=255)
                                                ccAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCg                                               >  2:1528617/1‑100 (MQ=255)
                                                ccAGGCGAACCTGCGTGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCg                                               <  2:506035/100‑1 (MQ=255)
                                                  aGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGc                                             >  1:661763/1‑100 (MQ=255)
                                                  aGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGc                                             >  1:662042/1‑100 (MQ=255)
                                                   ggCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCt                                            >  2:2085088/1‑100 (MQ=255)
                                                     cGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTcc                                          <  1:1064963/100‑1 (MQ=255)
                                                      gAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCg                                         >  2:1136814/1‑100 (MQ=255)
                                                       aaCCTGCTGGACAAAGATGGGCAGATCATCGACGGTGGGAAATCACGGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTGCgg                                        <  2:2202150/100‑1 (MQ=255)
                                                        aCCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGc                                       <  2:1071599/100‑1 (MQ=255)
                                                        aCCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGc                                       <  1:1830293/100‑1 (MQ=255)
                                                          ctgctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAg                                     <  2:273797/100‑1 (MQ=255)
                                                            gctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGGTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCg                                   >  2:1146318/1‑100 (MQ=255)
                                                             ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGc                                       >  2:186155/1‑95 (MQ=255)
                                                             ctgGATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCgg                                  <  2:224757/100‑1 (MQ=255)
                                                              tgGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGt                                 >  1:1454080/1‑100 (MQ=255)
                                                               ggATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGta                                >  2:1409655/1‑100 (MQ=255)
                                                               ggATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGta                                >  1:2358211/1‑100 (MQ=255)
                                                               ggATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGta                                <  1:2509166/100‑1 (MQ=255)
                                                               ggATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGta                                >  1:765330/1‑100 (MQ=255)
                                                               ggATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGACTGGTGGGTGCGGCTCCGGCAGCGGta                                >  2:2471610/1‑100 (MQ=255)
                                                                gATAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGtat                               <  2:1219496/100‑1 (MQ=255)
                                                                 aTAAAGATGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGtata                              <  2:697597/100‑1 (MQ=255)
                                                                        tGGGCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAg                       <  2:2211711/100‑1 (MQ=255)
                                                                          ggCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCCGCGGTATACATCGAGTc                     >  2:1793276/1‑100 (MQ=255)
                                                                          ggCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTc                     <  2:795500/100‑1 (MQ=255)
                                                                          ggCAGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTc                     >  2:2198539/1‑100 (MQ=255)
                                                                            cAGATCATCGTCGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTTCGGCTCCGGCAGCGGTATACATCGAGTCTg                   <  2:1688985/100‑1 (MQ=255)
                                                                             aGATCATCGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGACTgc                  >  1:211619/1‑100 (MQ=255)
                                                                              gATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcg                 <  2:1574087/100‑1 (MQ=255)
                                                                                tcatcGGCGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcggc               <  2:1687270/100‑1 (MQ=255)
                                                                                tcatcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcggc               >  1:776374/1‑100 (MQ=255)
                                                                                tcatcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcggc               <  1:2085102/100‑1 (MQ=255)
                                                                                 catcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTgcggcg              >  1:1023520/1‑100 (MQ=255)
                                                                                  atcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGcggcgg             <  1:836849/100‑1 (MQ=255)
                                                                                  atcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGcggcgg             <  1:2278136/100‑1 (MQ=255)
                                                                                  atcGACGGTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGcggcgg             <  2:2107713/100‑1 (MQ=255)
                                                                                         gTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggc      >  2:458510/1‑100 (MQ=255)
                                                                                         gTGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggc      >  1:1860917/1‑100 (MQ=255)
                                                                                          tGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcg     <  2:1889333/100‑1 (MQ=255)
                                                                                          tGGGAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcg     >  1:2153652/1‑100 (MQ=255)
                                                                                           gggAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcgg    <  2:2334597/100‑1 (MQ=255)
                                                                                           gggAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcgg    <  1:1043262/100‑1 (MQ=255)
                                                                                            ggAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggc   >  1:912940/1‑100 (MQ=255)
                                                                                             gAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggcg  <  2:2446919/100‑1 (MQ=255)
                                                                                             gAAATCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTAGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTAcggcggcn  <  1:141329/100‑2 (MQ=255)
                                                                                             gAAATCACTGACCACTGACTCCATGAGCAGCGTTATCTCTTGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACAACGAGTCTGCGGCGGGTAcggcggcg  >  2:1170727/1‑100 (MQ=255)
                                                                                                 |                                                                                               
GATGACGGCAGTATTTGATGCCACCGGAACTATCCGTGCCGTCGCCGGCCAGGCGAACCTGCTGGATAAAGATGGGCAGATCATCGACGGTGGGAAAGCACTGACCACTGACTCCATGAGCAGCGTTTTCTCTGGCCTGGTGGGTGCGGCTCCGGCAGCGGTATACATCGAGTCTGCGGCGGGTACGGCGGCG  >  NC_000913/4279273‑4279465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: