BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,340,287	G→A	100%	L79F (CTT→TTT)	adiA ←	arginine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,340,287	0	G	A	100.0%	367.1 / ‑6.7	117	L79F (CTT→TTT)	adiA	arginine decarboxylase
Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (51/63); total (53/64)

Only 100 of 117 total aligned reads displayed.

CATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAAT  >  NC_000913/4340191‑4340383

cATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTTTTTTTTTTTCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACtt                                                                                               >  2:1170784/1‑100 (MQ=255)

cATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACtt                                                                                               >  2:395921/1‑100 (MQ=255)

   cGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTAcc                                                                                            <  1:2265184/100‑1 (MQ=255)

   cGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTAcc                                                                                            <  1:2265339/100‑1 (MQ=255)

    gACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCg                                                                                           <  1:1253023/100‑1 (MQ=255)

    gACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCg                                                                                           >  1:1757089/1‑100 (MQ=255)

    gACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCg                                                                                           >  1:1716169/1‑100 (MQ=255)

      cAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGAt                                                                                         <  1:1657986/100‑1 (MQ=255)

      cAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGAt                                                                                         >  1:1774887/1‑100 (MQ=255)

       aaGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATc                                                                                        <  2:1510741/100‑1 (MQ=255)

        aGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCa                                                                                       >  2:2417573/1‑100 (MQ=255)

         gCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTTTTTTTTTTTTTACAGGAAGACCGGCACGTTTTGTTAACGCTTATTAAGCTTACCGATCa                                                                                       >  2:261236/1‑99 (MQ=255)

           tCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAAtt                                                                                    <  1:2438015/100‑1 (MQ=255)

            ccAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTg                                                                                   >  1:923649/1‑100 (MQ=255)

               gcagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCt                                                                                <  1:1634135/100‑1 (MQ=255)

               gcagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCt                                                                                <  2:406361/100‑1 (MQ=255)

               gcagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCt                                                                                <  1:2469670/100‑1 (MQ=255)

                cagGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTg                                                                               <  2:587630/100‑1 (MQ=255)

                cagGTCGCGATCCATTGCGGCGAGGGCTTTTTCACGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTg                                                                               <  2:713710/100‑1 (MQ=255)

                  ggTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGAc                                                                             >  2:1768193/1‑100 (MQ=255)

                  ggTCGCGATCCATGGCGGCCAGGGGTTTTTTCCGGTCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGAc                                                                             <  2:2419041/100‑1 (MQ=255)

                   gTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACg                                                                            >  1:1009527/1‑100 (MQ=255)

                   gTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACg                                                                            >  2:829666/1‑100 (MQ=255)

                       cgATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGtttt                                                                        <  2:2157906/100‑1 (MQ=255)

                        gATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCGAAGAGGAAGACCGGCACGTTTGGTTGGCGGTCATGAAACTTACCGATCAATTGTCTGACGgttt                                                                        <  2:1782800/99‑1 (MQ=255)

                           ccATTGCGGCGAGGGCTTTTTCAAGAGCGCACAACAGGTGGACGGGCACGTTTTGTTAGGGCGCATGAGAGTTGCCGATCAATtgtgtg                                                                               >  2:630813/1‑89 (MQ=255)

                             aTTGCGGAGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGtt                                                                  >  1:1350418/1‑100 (MQ=255)

                               tGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCg                                                                >  2:322358/1‑100 (MQ=255)

                               tGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCg                                                                <  2:149688/100‑1 (MQ=255)

                                  ggcgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCCATTGTCTGACGTTTTGATGTTCGTcc                                                             <  2:277638/100‑1 (MQ=255)

                                  ggcgAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTcc                                                             >  2:1249869/1‑100 (MQ=255)

                                     gAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGa                                                          >  2:1715804/1‑100 (MQ=255)

                                     gAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGa                                                          <  1:720779/100‑1 (MQ=255)

                                        ggCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGt                                                       >  1:581533/1‑100 (MQ=255)

                                          cTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTc                                                     <  2:2318479/100‑1 (MQ=255)

                                          cTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGCTTTGTTGGCGCTCCTGAAACTTACCAATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTc                                                     >  2:141127/1‑100 (MQ=255)

                                           tttttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTcc                                                    <  1:2181937/100‑1 (MQ=255)

                                           tttttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTATGACGTTTTGATGTTCGTACGGATGTTcc                                                    >  2:780081/1‑100 (MQ=255)

                                             tttCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAt                                                  >  2:2008543/1‑100 (MQ=255)

                                              ttCCCGGTCGCCCAACGGGAAGACCGGAACGTTTTGTTGGCGCTCGTGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAtt                                                 <  2:1499649/100‑1 (MQ=255)

                                               tCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAttt                                                <  2:2061393/100‑1 (MQ=255)

                                               tCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAttt                                                <  2:618557/100‑1 (MQ=255)

                                               tCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCAttt                                                <  2:2424496/100‑1 (MQ=255)

                                                cccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTg                                               <  2:650192/100‑1 (MQ=255)

                                                cccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTg                                               <  1:1913577/100‑1 (MQ=255)

                                                 ccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGa                                              <  2:1933802/100‑1 (MQ=255)

                                                 ccGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGa                                              <  1:681975/100‑1 (MQ=255)

                                                  cGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGAt                                             <  1:1157844/100‑1 (MQ=255)

                                                  cGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGAt                                             >  2:327520/1‑100 (MQ=255)

                                                     tCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGc                                          <  2:845872/100‑1 (MQ=255)

                                                      cGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCt                                         >  1:850306/1‑100 (MQ=255)

                                                       gCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTg                                        >  1:110679/1‑100 (MQ=255)

                                                       gCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTg                                        >  2:1758130/1‑100 (MQ=255)

                                                       gCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCATCCGGATGTTCCATTTGATAGc                                          >  2:1456133/1‑98 (MQ=255)

                                                         ccAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGaa                                      <  1:2249673/100‑1 (MQ=255)

                                                          cAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAAc                                     <  2:1772389/100‑1 (MQ=255)

                                                          cAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCAGGATGTTCCATTTGATAGCttcac                                     >  2:2190785/1‑96 (MQ=255)

                                                           aacaataaGACCGGCACGTTTTGTTGTCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGTATGTTCCATTTGATAGCTg                                        <  2:2225259/90‑1 (MQ=255)

                                                           aaCAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACa                                    <  1:1715816/100‑1 (MQ=255)

                                                           aaCAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACGTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACa                                    <  2:193503/100‑1 (MQ=255)

                                                              aGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCa                                 <  1:2028814/100‑1 (MQ=255)

                                                               ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAAGATCAg                                >  2:1239349/1‑100 (MQ=255)

                                                               ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                                <  1:1623579/100‑1 (MQ=255)

                                                               ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                                >  1:2271608/1‑100 (MQ=255)

                                                               ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                                >  1:121987/1‑100 (MQ=255)

                                                               ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                                >  1:1975340/1‑100 (MQ=255)

                                                               ggAAGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTAGTCCGGATGTTCCATTTGATAGCTGAACATCAg                                <  2:1543845/100‑1 (MQ=255)

                                                                 aacaCCGGCACGTTTGGTTGGCGCTCGTGAAACTTACCGATGAATTGTCTGACGTTTTGATGTTCGTCCGGAGGTTCCATTTCATAGCTGAACATCAGGc                              <  2:198855/97‑1 (MQ=255)

                                                                  aGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCa                             <  2:2035593/100‑1 (MQ=255)

                                                                  aGACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTCTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAg                                >  2:2555410/1‑97 (MQ=255)

                                                                   gACCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAg                            <  1:2498967/100‑1 (MQ=255)

                                                                    aCCGGCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGt                           >  2:83910/1‑100 (MQ=255)

                                                                     ccGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTGCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTc                          <  2:1608074/100‑1 (MQ=255)

                                                                       ggCACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCaa                        >  1:236657/1‑100 (MQ=255)

                                                                         cACGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATg                      <  1:902214/100‑1 (MQ=255)

                                                                           cGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGCACATCAGGCCGTCAATGGc                    >  1:699958/1‑100 (MQ=255)

                                                                           cGTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGc                    <  1:549367/100‑1 (MQ=255)

                                                                            gTTTTGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCt                   <  1:170115/100‑1 (MQ=255)

                                                                             ttttGTTGGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCtt                  >  2:54391/1‑100 (MQ=255)

                                                                                tgttgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGt               >  1:5574/1‑100 (MQ=255)

                                                                                 gttgGCGGTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGtt              >  1:1347066/1‑100 (MQ=255)

                                                                                 gttgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGtt              <  1:1889532/100‑1 (MQ=255)

                                                                                 gttgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGtt              >  1:1709859/1‑100 (MQ=255)

                                                                                 gttgGCGCTCATGAAACTTACCGAGCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGtt              <  2:639986/100‑1 (MQ=255)

                                                                                   tgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTg            <  2:1980170/100‑1 (MQ=255)

                                                                                   tgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTg            <  2:1167072/100‑1 (MQ=255)

                                                                                   tgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTg            >  2:150059/1‑100 (MQ=255)

                                                                                   tgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTg            >  2:1574235/1‑100 (MQ=255)

                                                                                   tgGCGCTCATGAAACTTACCGATCAATTGTCTGACGTTTGGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTg            <  2:1166652/100‑1 (MQ=255)

                                                                                      cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCGATGGCTTCGTTTGAag         <  2:1199155/100‑1 (MQ=255)

                                                                                      cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag         <  1:1832452/100‑1 (MQ=255)

                                                                                      cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag         <  1:595096/100‑1 (MQ=255)

                                                                                      cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag         <  1:662548/100‑1 (MQ=255)

                                                                                      cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag         <  1:1179573/100‑1 (MQ=255)

                                                                                      cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag         <  1:1114805/100‑1 (MQ=255)

                                                                                      cgcTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAag         <  1:776165/100‑1 (MQ=255)

                                                                                        cTCATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagag       <  1:2378678/100‑1 (MQ=255)

                                                                                          cATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagagag     >  2:2521247/1‑100 (MQ=255)

                                                                                          cATGAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAagagag     >  2:180433/1‑100 (MQ=255)

                                                                                             gAAACTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAAt  >  2:1849199/1‑100 (MQ=255)

CATCGACAAGCTCCAGCAGGTCGCGATCCATTGCGGCGAGGGCTTTTTCCCGATCGCCCAACAGGAAGACCGGCACGTTTTGTTGGCGCTCATGAAGCTTACCGATCAATTGTCTGACGTTTTGATGTTCGTCCGGATGTTCCATTTGATAGCTGAACATCAGGCAGTCAATGGCTTCGTTTGAAGAGAGAAT  >  NC_000913/4340191‑4340383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`