Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,360,814 T→G 100% Y374S (TAC→TCC)  cadC ← cadBA operon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,360,8140TG97.6% 344.4 / ‑3.6 107Y374S (TAC→TCC) cadCcadBA operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base G (57/45);  total (60/47)
Fisher's exact test for biased strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

GTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGCC  >  NC_000913/4360717‑4360909
                                                                                                 |                                                                                               
gTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAg                                                                                               >  1:1610732/1‑100 (MQ=255)
gTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAg                                                                                               >  1:1623294/1‑100 (MQ=255)
   aTCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTa                                                                                            >  1:765536/1‑100 (MQ=255)
   aTCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTa                                                                                            >  1:1779665/1‑100 (MQ=255)
     cTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTaaa                                                                                          >  2:2064666/1‑100 (MQ=255)
      tATTTCTGTGTTAAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAAt                                                                                         <  2:1930049/100‑1 (MQ=255)
       aTTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAAtt                                                                                        <  2:44945/100‑1 (MQ=255)
       aTTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAAtt                                                                                        >  1:767805/1‑100 (MQ=255)
       aTTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAAtt                                                                                        >  2:2148975/1‑100 (MQ=255)
        tttCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTc                                                                                       >  1:658796/1‑100 (MQ=255)
            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                   <  1:365901/100‑1 (MQ=255)
            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                   <  1:495090/100‑1 (MQ=255)
            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                   >  2:154559/1‑100 (MQ=255)
            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                   <  1:2098954/100‑1 (MQ=255)
            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTAGCGGAGGTAAATTCTggg                                                                                   >  1:273856/1‑100 (MQ=255)
            tgtgTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATAACAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                   <  2:1401266/100‑1 (MQ=255)
             gtgtagaGTGCTTCTAATTGGTTTTTATCTAAATGTTTTTGAGAATGGCTTACGATACCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTggg                                                                                   <  2:1400325/93‑1 (MQ=255)
             gtgtTCAGTGCTGCTAATTTGTTTTCATCTAAAGAATGTTGAGAATGGCGCACGATATAAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTg                                                                                     <  2:1275265/97‑1 (MQ=255)
             gtgtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgggg                                                                                  <  2:2512070/100‑1 (MQ=255)
              tgtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTgggg                                                                                  <  2:581786/99‑1 (MQ=255)
               gtTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGAAAATTCTGGGgat                                                                                >  2:1179278/1‑100 (MQ=255)
                   aGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGAtt                                                                            >  2:1883991/1‑100 (MQ=255)
                     tgctgcTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATCCTTTTTCTGCTCTCGCCTAGGTCTATTCTtgggctcgct                                                                            >  2:2354811/1‑92 (MQ=255)
                      gctgctAATTGTTTTTCCTCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCAGAGGTAAATTCTGGGGATGAtt                                                                            >  2:946290/1‑97 (MQ=255)
                      gctgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGaa                                                                         <  2:2236619/100‑1 (MQ=255)
                      gctgctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGAATATTCTGGGGATGATTGaa                                                                         >  2:865086/1‑100 (MQ=255)
                        agctAATTGTATTTCACCTAAAGGATGTAAAGGATGTCGCACTAGTTATACTCATGCTTTTTCTGCTTGAGCCCAGGTAAATTCTGGGGATGATTGAAc                                                                        <  2:2406883/98‑1 (MQ=255)
                         gctAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACaa                                                                      >  1:1508390/1‑100 (MQ=255)
                           tAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtc                                                                    >  2:997326/1‑100 (MQ=255)
                           tAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAAtc                                                                    <  1:1075134/100‑1 (MQ=255)
                              ttGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGGTTGAACAATCTCa                                                                 <  1:1721119/100‑1 (MQ=255)
                              ttGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCa                                                                 >  1:2508168/1‑100 (MQ=255)
                                gTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATTAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTCGGGATGATTGAACAATCTCAc                                                                >  1:1457438/1‑99 (MQ=255)
                                 tttttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATTTCACCt                                                              >  1:2418911/1‑100 (MQ=255)
                                 tttttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCt                                                              <  1:714759/100‑1 (MQ=255)
                                  ttttCATCTAGAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCt                                                              <  2:1522881/99‑1 (MQ=255)
                                   tttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCtaa                                                            >  1:2399426/1‑100 (MQ=255)
                                   tttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCtaa                                                            >  2:1538562/1‑100 (MQ=255)
                                    ttCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCtaat                                                           <  2:31924/100‑1 (MQ=255)
                                     tCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCtaata                                                          <  1:573565/100‑1 (MQ=255)
                                      cATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCtaataa                                                         >  1:2268619/1‑100 (MQ=255)
                                        tCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAAtt                                                       <  2:1412643/100‑1 (MQ=255)
                                         cTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTc                                                      >  1:2241852/1‑100 (MQ=255)
                                          tAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCa                                                     >  2:787323/1‑100 (MQ=255)
                                          tAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCa                                                     >  1:470267/1‑100 (MQ=255)
                                          tAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCa                                                     >  2:1248153/1‑100 (MQ=255)
                                           aaaGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCAc                                                    <  1:2193732/100‑1 (MQ=255)
                                             aGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTg                                                  <  2:2197963/100‑1 (MQ=255)
                                               gATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGc                                                >  2:1598364/1‑100 (MQ=255)
                                                 tGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCAc                                              <  1:2318832/100‑1 (MQ=255)
                                                 tGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCAc                                              <  2:2026735/100‑1 (MQ=255)
                                                 cttccgatctGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCAc                                              <  1:1378074/91‑1 (MQ=255)
                                                  gTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACg                                             <  1:735/100‑1 (MQ=255)
                                                  gTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACg                                             >  1:2397701/1‑100 (MQ=255)
                                                    tGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGt                                           >  1:1477495/1‑100 (MQ=255)
                                                    tGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGt                                           >  1:2209254/1‑100 (MQ=255)
                                                     gagaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGtt                                          >  1:1073793/1‑100 (MQ=255)
                                                     gagaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGtt                                          >  2:692675/1‑100 (MQ=255)
                                                     gagaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGtt                                          >  2:935708/1‑100 (MQ=255)
                                                      agaATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTc                                         <  1:2042969/100‑1 (MQ=255)
                                                           ggCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCAcagatcggaag                                    >  2:1378065/1‑90 (MQ=255)
                                                              gcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAttt                                 <  1:536405/100‑1 (MQ=255)
                                                              gcACGATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGAttt                                 <  1:2258808/100‑1 (MQ=255)
                                                               cACGATATCAACTAATGCTTTTTTTTTTTTTTTTTAGGTCAATTCTGGGGATGTTTTTATATTCTCACATAATAATTCACTGGCACGGTTCAATGAttt                                 >  2:1689547/1‑99 (MQ=255)
                                                                aagATATCAACTAATGATTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTAAATGAttt                                 <  2:695922/96‑1 (MQ=255)
                                                                  gATATCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTatca                             <  2:1823907/100‑1 (MQ=255)
                                                                     atCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCg                          <  1:687649/100‑1 (MQ=255)
                                                                      tCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCg                          <  2:1224891/99‑1 (MQ=255)
                                                                      tCAACTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTAAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGc                         >  1:920771/1‑100 (MQ=255)
                                                                       cAACTAATGCTTTATCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGcc                        <  2:2053773/100‑1 (MQ=255)
                                                                        aaCTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCa                       >  1:1864051/1‑100 (MQ=255)
                                                                        aaCTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCa                       <  2:470263/100‑1 (MQ=255)
                                                                        aaCTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCa                       >  2:1784228/1‑100 (MQ=255)
                                                                         aCTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCAt                      <  2:2236558/100‑1 (MQ=255)
                                                                         aCTAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCAt                      >  1:108931/1‑100 (MQ=255)
                                                                          cTAATGCTTTTTCTGCTCTCGCGGAGGTCAATTCTAGGGATGATTGAACAATCTCACCTACTAATTCACTAGAACGGGTCAATGATTTATCATCGCCAt                      >  2:995390/1‑99 (MQ=255)
                                                                           tAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGc                    <  1:1925130/100‑1 (MQ=255)
                                                                           tAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGc                    <  1:1604821/100‑1 (MQ=255)
                                                                           tAATGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTACCATCGCCATGc                    <  1:630108/100‑1 (MQ=255)
                                                                            aaTGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGc                    >  2:1988796/1‑99 (MQ=255)
                                                                            aaTGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCa                   >  1:702923/1‑100 (MQ=255)
                                                                            aaTGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCa                   <  1:1838874/100‑1 (MQ=255)
                                                                            aaTGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTAAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCa                   >  2:471958/1‑100 (MQ=255)
                                                                             attcTTTTTCTGCTCTCGGGGATGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGTACGGTTTAATGATTTATCATCGCCATGCa                   <  2:406678/96‑1 (MQ=255)
                                                                             aTGCTTTTTCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAg                  >  2:2253823/1‑100 (MQ=255)
                                                                                 tttttCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTaaa              <  2:1422571/100‑1 (MQ=255)
                                                                                   tttCTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCACTAaata            >  2:639326/1‑100 (MQ=255)
                                                                                    ttCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAaataa           >  2:1426451/1‑100 (MQ=255)
                                                                                      cTGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATc         >  2:1621367/1‑100 (MQ=255)
                                                                                       tGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCa        >  2:1067877/1‑100 (MQ=255)
                                                                                       tGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCa        <  2:658789/100‑1 (MQ=255)
                                                                                       tGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCa        <  2:1779652/100‑1 (MQ=255)
                                                                                       tGCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATAGCCATGCAGTAAATAATCa        >  2:1756181/1‑100 (MQ=255)
                                                                                        gCTCTCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAt       >  2:325491/1‑100 (MQ=255)
                                                                                         ctctCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAtg      <  2:731745/100‑1 (MQ=255)
                                                                                         ctctCGCGGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCAtg      >  1:364368/1‑100 (MQ=255)
                                                                                             cgcgGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGcc  >  1:2283128/1‑100 (MQ=255)
                                                                                             cgcgGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGcc  <  2:733081/100‑1 (MQ=255)
                                                                                             cgcgGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGcc  >  2:1998286/1‑100 (MQ=255)
                                                                                              gcgGAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGcc  <  2:925681/99‑1 (MQ=255)
                                                                                                 |                                                                                               
GTTATCTATTTCTGTGTTCAGTGCTGCTAATTGTTTTTCATCTAAAGGATGTTGAGAATGGCGCACGATATCAACTAATGCTTTTTCTGCTCTCGCGTAGGTAAATTCTGGGGATGATTGAACAATCTCACCTAATAATTCACTGGCACGGTTCAATGATTTATCATCGCCATGCAGTAAATAATCATGTGCC  >  NC_000913/4360717‑4360909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: