Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 400,214 T→C 100% D237G (GAC→GGC)  ddlA ← D‑alanine‑D‑alanine ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913400,2140TC95.7% 262.1 / ‑1.6 83D237G (GAC→GGC) ddlAD‑alanine‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base C (35/43);  total (38/45)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTG  >  NC_000913/400117‑400310
                                                                                                 |                                                                                                
tACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCg                                                                                                <  1:1383696/100‑1 (MQ=255)
 aCCACTTTCGCGCCATCTTCGTCCATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTTGCTTGCGGATTGCCGt                                                                                               >  2:537031/1‑100 (MQ=255)
 aCCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGt                                                                                               >  1:1898777/1‑100 (MQ=255)
 aCCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGt                                                                                               >  1:1426785/1‑100 (MQ=255)
    aCTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGc                                                                                            <  1:1761577/100‑1 (MQ=255)
    aCTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGc                                                                                            <  2:2524846/100‑1 (MQ=255)
     cTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGc                                                                                            <  1:2276719/99‑1 (MQ=255)
      tttCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGccc                                                                                          >  2:1353127/1‑100 (MQ=255)
      tttCGCGCCATCTTCGTCAATGTACTTGCTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTTcc                                                                                           <  2:395608/99‑1 (MQ=255)
        tCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACCGGTGCTGGCTTGCGGATTGCCGTTGCCCAg                                                                                        >  1:1245045/1‑100 (MQ=255)
         cgcgCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGa                                                                                       <  1:1000189/100‑1 (MQ=255)
         cgcgCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGa                                                                                       >  2:770147/1‑100 (MQ=255)
          gcgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGaa                                                                                      >  2:2216622/1‑100 (MQ=255)
          gcgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAgca                                                                                      >  2:2499993/1‑98 (MQ=255)
           cgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAAc                                                                                     <  2:568032/100‑1 (MQ=255)
           cgcCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAAc                                                                                     <  1:930640/100‑1 (MQ=255)
             ccATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATATCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTg                                                                                   >  2:1635863/1‑100 (MQ=255)
              cATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTgc                                                                                  >  1:1509972/1‑100 (MQ=255)
               aTCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGTTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTgcg                                                                                 <  2:2012135/100‑1 (MQ=255)
                 cTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCa                                                                               <  2:248544/100‑1 (MQ=255)
                 cTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCa                                                                               <  1:27322/100‑1 (MQ=255)
                    cGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTc                                                                            >  1:1604090/1‑100 (MQ=255)
                      tCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGa                                                                          <  2:1114024/100‑1 (MQ=255)
                       cAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAt                                                                         >  2:905071/1‑100 (MQ=255)
                         aTGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAtct                                                                       <  2:2148977/100‑1 (MQ=255)
                         aTGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAtct                                                                       <  2:2148704/100‑1 (MQ=255)
                          tGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAtctc                                                                      <  2:2196010/100‑1 (MQ=255)
                          tGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGAtctc                                                                      <  2:1033273/100‑1 (MQ=255)
                           gTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCa                                                                     <  1:1043323/100‑1 (MQ=255)
                           gTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTAGATCTCa                                                                     >  1:2393944/1‑100 (MQ=255)
                            tACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCAc                                                                    >  1:1811362/1‑100 (MQ=255)
                                      cGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGAt                                                          >  1:2237304/1‑100 (MQ=255)
                                      cGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCCATCTCCCGACCttt                                                             >  1:277330/1‑97 (MQ=255)
                                            cATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTg                                                    >  2:922630/1‑100 (MQ=255)
                                            cATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTg                                                    <  1:172449/100‑1 (MQ=255)
                                             aTAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGc                                                   >  1:2003370/1‑100 (MQ=255)
                                              tAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGACCTGAGCATTCGATCTCACGACCTTTGATCCCTTGCt                                                  >  1:1492804/1‑100 (MQ=255)
                                                gAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCa                                                >  1:725528/1‑100 (MQ=255)
                                                 aaaTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCaa                                               <  1:1630078/100‑1 (MQ=255)
                                                  aaTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAAc                                              <  1:1166597/100‑1 (MQ=255)
                                                  aaTCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAAc                                              >  1:899658/1‑100 (MQ=255)
                                                     cGCTGGTGATTACGATCTCTCCACATGTGCTTGCTTGCTGAGTGGCTTTGCCGAGAACTGCGCATTCGATCTCACGACCTTGGAt                                                          <  2:2268876/85‑1 (MQ=255)
                                                      gCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATc                                          <  1:1660542/100‑1 (MQ=255)
                                                      gCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATc                                          <  1:2077749/100‑1 (MQ=255)
                                                      gCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATc                                          <  2:1648153/100‑1 (MQ=255)
                                                       cTGGTGAGTACGATCTCTCCGCAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCa                                         >  2:1561792/1‑100 (MQ=255)
                                                       cTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATc                                          <  1:1402589/99‑1 (MQ=255)
                                                        tGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCAc                                        <  2:2385124/100‑1 (MQ=255)
                                                         ggTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACt                                       <  1:714208/100‑1 (MQ=255)
                                                         ggTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACt                                       <  1:714174/100‑1 (MQ=255)
                                                            gAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTa                                    <  2:1923778/100‑1 (MQ=255)
                                                             aGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTAt                                   <  1:2216636/100‑1 (MQ=255)
                                                             aGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTAt                                   <  1:873120/100‑1 (MQ=255)
                                                             aGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTAt                                   <  1:873568/100‑1 (MQ=255)
                                                              gTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCCGAACTGCGCATTCGATCTCACGCCCTTTGATCCCTTGCTCAACGATCACTTTATg                                  >  1:619359/1‑100 (MQ=255)
                                                              gTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATg                                  >  2:1987276/1‑100 (MQ=255)
                                                              gTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATg                                  >  1:92222/1‑100 (MQ=255)
                                                               tACGATCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGa                                 <  1:2001831/100‑1 (MQ=255)
                                                                aCGATCTCTCCACAGGTGCTGGCTTGCTGATTGCCGTTGCCCCGAGCTGCGCATTCGAGCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTAt                                   <  2:2256112/97‑1 (MQ=255)
                                                                   aTCTCTCCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcga                             >  2:1228277/1‑100 (MQ=255)
                                                                        tcCACAGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcg                        <  1:1571435/100‑1 (MQ=255)
                                                                         ccACAGGTGCTGGCTTACGGATTGTCGTTGACCAGAACGGCGCATTCGATCTCCCGACCTTTAATCCCTTGCTCCACGATCACTTTATGAtcgaactcga                       >  2:193739/1‑100 (MQ=255)
                                                                          cacaGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcgaa                      <  2:206349/100‑1 (MQ=255)
                                                                          cacaGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcgaa                      >  1:1629000/1‑100 (MQ=255)
                                                                           acaGGTGCTTGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGAtcgaactcgaac                     <  2:2084107/100‑1 (MQ=255)
                                                                           acaGGTGCTGGATTGCGGATTGCCGTTGCCCAGAACGTCGCATTCGGTCTGACGGCCATTGGTCACTTGCTCAACGATCACTTTAAGAtctatagcgga                      >  2:2211165/1‑90 (MQ=255)
                                                                            caGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACg                    <  2:1263284/100‑1 (MQ=255)
                                                                             aGGTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGc                   >  1:530503/1‑100 (MQ=255)
                                                                              ggTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGcc                  >  1:1374616/1‑100 (MQ=255)
                                                                              ggTGCTGGCTTGAGGATTGCCGTTGCCCAGAAGTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGcn                  <  2:2345850/100‑2 (MQ=255)
                                                                               gTGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCa                 <  1:1174736/100‑1 (MQ=255)
                                                                                tGCTGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAg                <  1:1635875/100‑1 (MQ=255)
                                                                                   tGGCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATc             <  1:2393030/100‑1 (MQ=255)
                                                                                     gCTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGa           >  2:2515682/1‑100 (MQ=255)
                                                                                      cTTGCGGGTTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGAc          >  1:939809/1‑100 (MQ=255)
                                                                                      cTTGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGAc          >  1:40436/1‑100 (MQ=255)
                                                                                        tGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACgg        >  1:2383796/1‑100 (MQ=255)
                                                                                        tGCGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACgg        <  2:1898763/100‑1 (MQ=255)
                                                                                          cGGATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCa      >  1:289520/1‑100 (MQ=255)
                                                                                           ggATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCaa     <  1:2485692/100‑1 (MQ=255)
                                                                                            gATTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAAt    >  1:853364/1‑100 (MQ=255)
                                                                                             aTTGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAAtt   <  2:2295307/100‑1 (MQ=255)
                                                                                              ttGCCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTg  >  2:354030/1‑100 (MQ=255)
                                                                                                 |                                                                                                
TACCACTTTCGCGCCATCTTCGTCAATGTACTTGGTGTCGTAGGCATAGAAATCGCTGGTGAGTACGATCTCTCCACAGGTGCTGGCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTG  >  NC_000913/400117‑400310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑