BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	428,188	G→T	100%	R181L (CGC→CTC)	secD →	SecYEG protein translocase auxillary subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	428,188	0	G	T	100.0%	391.2 / NA	117	R181L (CGC→CTC)	secD	SecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (53/64); total (53/64)

Only 100 of 117 total aligned reads displayed.

AAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGG  >  NC_000913/428092‑428283

aaaaTATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGa                                                                                              >  1:185753/1‑100 (MQ=255)

  aaTATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATg                                                                                            >  1:1419810/1‑100 (MQ=255)

   atatCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGc                                                                                           <  2:1254030/100‑1 (MQ=255)

     atCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTa                                                                                         >  1:133504/1‑100 (MQ=255)

            gCCTACGCAGTGACCTGCGCGCCAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCCCg                                                                                  >  2:178487/1‑100 (MQ=255)

             ccTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGt                                                                                 <  1:1145974/100‑1 (MQ=255)

              cTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtg                                                                                <  1:1832323/100‑1 (MQ=255)

               tACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtga                                                                               <  2:216051/100‑1 (MQ=255)

               tACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtga                                                                               >  1:2045426/1‑100 (MQ=255)

                  gCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGtgatga                                                                            >  1:1708063/1‑100 (MQ=255)

                   cAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGaa                                                                           >  1:976140/1‑100 (MQ=255)

                    aGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAg                                                                          <  1:1351675/100‑1 (MQ=255)

                     gTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGc                                                                         <  2:2037678/100‑1 (MQ=255)

                     gTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGc                                                                         <  2:433863/100‑1 (MQ=255)

                       gACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCa                                                                       >  2:891900/1‑100 (MQ=255)

                         ccTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAACACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCAtt                                                                     >  1:791981/1‑100 (MQ=255)

                         ccTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCAtt                                                                     <  2:2299043/100‑1 (MQ=255)

                         ccTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCAtt                                                                     >  1:2336050/1‑100 (MQ=255)

                          cTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTg                                                                    <  2:2348778/100‑1 (MQ=255)

                           tGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGc                                                                   >  2:114636/1‑100 (MQ=255)

                            gcgcGAAAAGTGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTTCTCGATGCTAAAGCTCGTGATGAAGCCATTGCg                                                                  <  2:1681389/100‑1 (MQ=255)

                            gcgcGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAACCTCGTGATGAAGCCATTGCg                                                                  <  2:1564665/100‑1 (MQ=255)

                            gcgcAAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCg                                                                  <  2:1051771/100‑1 (MQ=255)

                             cgcgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGt                                                                 >  1:2310999/1‑100 (MQ=255)

                              gcgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTa                                                                <  1:685956/100‑1 (MQ=255)

                               cgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTAt                                                               >  2:90926/1‑100 (MQ=255)

                               cgAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTAt                                                               >  1:244895/1‑100 (MQ=255)

                                gAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATc                                                              >  1:1984653/1‑100 (MQ=255)

                                 aaaaGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCt                                                             >  1:2258923/1‑100 (MQ=255)

                                 aaaaGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCt                                                             <  2:229891/100‑1 (MQ=255)

                                    aGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAg                                                          >  1:1485196/1‑100 (MQ=255)

                                     gggCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGc                                                         >  1:1759140/1‑100 (MQ=255)

                                      ggCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCa                                                        >  2:757113/1‑100 (MQ=255)

                                         aTCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAgc                                                     >  2:192432/1‑100 (MQ=255)

                                          tCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAgcg                                                    <  2:915194/100‑1 (MQ=255)

                                          tCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAgcg                                                    >  2:1219835/1‑100 (MQ=255)

                                          tCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAgcg                                                    >  1:1775068/1‑100 (MQ=255)

                                          tCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAgcg                                                    <  1:2350978/100‑1 (MQ=255)

                                             cgatctccACTGTTCGTAAAGAAAACAACTACGGCCTGGGCATCACTTTCCTCGAGGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAgcgc                                                   <  2:836090/92‑1 (MQ=255)

                                                ataCCACTGTTCGTAAAGAAAACAACTACGGCCTGAGTATCACTTTCGTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATcc                                              <  2:1727468/100‑1 (MQ=255)

                                                ataCCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATcc                                              <  2:2276198/100‑1 (MQ=255)

                                                ataCCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATcc                                              <  2:718722/100‑1 (MQ=255)

                                                ataCCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATcc                                              >  1:274782/1‑100 (MQ=255)

                                                   ccACTGTTCGTAAAGAAAACAACTACGGCCTGGGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAgcgcagatcgg                                            >  1:836097/1‑92 (MQ=255)

                                                   ccACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGa                                           >  2:2449531/1‑100 (MQ=255)

                                                    cACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGAc                                          <  1:1547591/100‑1 (MQ=255)

                                                     aCTGTTCGTAAAGAAAGCAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGAcc                                         <  2:1495506/100‑1 (MQ=255)

                                                     aCTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGAcc                                         <  2:899575/100‑1 (MQ=255)

                                                     aCTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGAcc                                         <  1:739802/100‑1 (MQ=255)

                                                      cTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCt                                        >  1:1931546/1‑100 (MQ=255)

                                                      cTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCt                                        <  2:1435914/100‑1 (MQ=255)

                                                       tGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTTCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTg                                       <  1:259359/100‑1 (MQ=255)

                                                        gTTCGTAAAGAAAACAACTACGGCCTGCGCATCACTTTCCTCGATGCTAAAGCGCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTg                                       <  2:2352441/99‑1 (MQ=255)

                                                        gTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTgg                                      >  1:2552409/1‑100 (MQ=255)

                                                        gTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTgg                                      >  1:2379286/1‑100 (MQ=255)

                                                        gTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTgg                                      >  1:299158/1‑100 (MQ=255)

                                                         ttCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGt                                     <  1:1619691/100‑1 (MQ=255)

                                                         ttCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGt                                     <  2:1911496/100‑1 (MQ=255)

                                                         ttCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGt                                     <  1:854707/100‑1 (MQ=255)

                                                          tCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTg                                    <  2:1535180/100‑1 (MQ=255)

                                                           cGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGa                                   <  1:627109/100‑1 (MQ=255)

                                                           cGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGa                                   >  2:2553138/1‑100 (MQ=255)

                                                           cGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGa                                   <  2:507311/100‑1 (MQ=255)

                                                            gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                  <  2:1619815/100‑1 (MQ=255)

                                                            gTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAt                                  <  2:1646596/100‑1 (MQ=255)

                                                             tAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCGGGACCTGGTGa                                   >  2:1517075/1‑98 (MQ=255)

                                                             tAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAtt                                 >  1:432821/1‑100 (MQ=255)

                                                             tAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAtt                                 <  1:901610/100‑1 (MQ=255)

                                                             tAAAGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGAtt                                 <  2:1052281/100‑1 (MQ=255)

                                                              aaaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTa                                <  1:926704/100‑1 (MQ=255)

                                                               aaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTag                               <  2:1109390/100‑1 (MQ=255)

                                                               aaGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTag                               <  1:1491900/100‑1 (MQ=255)

                                                                aGAAAACAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagc                              <  1:1332949/100‑1 (MQ=255)

                                                                  aagacaacTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagcag                            <  1:942291/97‑1 (MQ=255)

                                                                  aaaaCAACTACGGCCTGAGCATCACTTTGCTCGATGCTAAAGCTCGTGATGAAGCCATTCCGTATCTGAGCAAGAGCCATCCGGACCTGTCGATTagca                             >  2:885950/1‑99 (MQ=255)

                                                                  aaaaCAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagcag                            <  1:631999/100‑1 (MQ=255)

                                                                  aaaaCAACTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTagcag                            <  1:1615278/100‑1 (MQ=255)

                                                                    aacaacTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGcc                          >  1:1251555/1‑100 (MQ=255)

                                                                     acaacTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCa                         >  1:499925/1‑100 (MQ=255)

                                                                        acTACGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAggg                      <  1:954485/100‑1 (MQ=255)

                                                                           aCGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcag                   <  2:332121/100‑1 (MQ=255)

                                                                           aCGGCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcag                   <  1:1391569/100‑1 (MQ=255)

                                                                             ggCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcagca                 >  2:297551/1‑100 (MQ=255)

                                                                             ggCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGgcagca                 <  2:1344822/100‑1 (MQ=255)

                                                                              gCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCaa                >  1:2183713/1‑100 (MQ=255)

                                                                              gCCTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGAAGCCAGGGCAGCaa                >  2:80714/1‑100 (MQ=255)

                                                                                cTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAAcc              >  1:500921/1‑100 (MQ=255)

                                                                                cTGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAAcc              >  1:878985/1‑100 (MQ=255)

                                                                                 tGAGCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCa             <  2:2552393/100‑1 (MQ=255)

                                                                                    gCATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCCGCCAGGGCAGCAACCAGCt          >  2:820411/1‑100 (MQ=255)

                                                                                     cATCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtg         <  1:1469538/100‑1 (MQ=255)

                                                                                      aTCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCTATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgc        >  2:1794227/1‑100 (MQ=255)

                                                                                      aTCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgc        <  1:919306/100‑1 (MQ=255)

                                                                                       tCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcg       >  2:1334713/1‑100 (MQ=255)

                                                                                       tCACTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcg       >  2:2410239/1‑100 (MQ=255)

                                                                                         aCTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtg     <  1:1087767/100‑1 (MQ=255)

                                                                                          cTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtgc    <  1:2224367/100‑1 (MQ=255)

                                                                                          cTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtgc    >  2:1503693/1‑100 (MQ=255)

                                                                                          cTTTCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCtgcgtgc    >  1:1197432/1‑100 (MQ=255)

                                                                                            ttCCTCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCgg  <  1:1885878/100‑1 (MQ=255)

AAAATATCGATAGCCTACGCAGTGACCTGCGCGAAAAGGGCATCCCGTATACCACTGTTCGTAAAGAAAACAACTACGGCCTGAGCATCACTTTCCGCGATGCTAAAGCTCGTGATGAAGCCATTGCGTATCTGAGCAAGCGCCATCCGGACCTGGTGATTAGCAGCCAGGGCAGCAACCAGCTGCGTGCGG  >  NC_000913/428092‑428283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`