Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 53,012 A→G 100% G135G (GGT→GGC)  pdxA ← 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091353,0120AG99.0% 355.5 / ‑4.9 104G135G (GGT→GGC) pdxA4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (44/57);  total (45/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 104 total aligned reads displayed.

GTAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACG  >  NC_000913/52915‑53106
                                                                                                 |                                                                                              
gTAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGcc                                                                                              <  2:547772/100‑1 (MQ=255)
  aaaTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTTTGGCCgg                                                                                            <  1:1349469/100‑1 (MQ=255)
  aaaTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCgg                                                                                            <  2:446537/100‑1 (MQ=255)
  aaaTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCgg                                                                                            <  1:306447/100‑1 (MQ=255)
  aaaTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCgg                                                                                            <  1:306455/100‑1 (MQ=255)
   aaTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGt                                                                                           >  1:1761264/1‑100 (MQ=255)
   aaTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGt                                                                                           <  2:579273/100‑1 (MQ=255)
    aTGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTa                                                                                          <  2:1138095/100‑1 (MQ=255)
     tGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCTTTTTTTTTCGCTCTTCGAAAAACTCGGTATGGCCGGTaa                                                                                         >  2:1549/1‑100 (MQ=255)
     tGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTaa                                                                                         >  2:2541261/1‑100 (MQ=255)
     tGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTaa                                                                                         >  1:2071564/1‑100 (MQ=255)
       cgtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTaaaa                                                                                       <  2:1863522/100‑1 (MQ=255)
        gtcgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCGCTTCGAAAAACTCGGCATGCCCGGTACAAg                                                                                      >  2:463432/1‑100 (MQ=255)
          cgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGa                                                                                    >  1:1830407/1‑100 (MQ=255)
          cgtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGa                                                                                    >  1:1597135/1‑100 (MQ=255)
           gtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGaa                                                                                   >  1:1291305/1‑100 (MQ=255)
           gtTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGaa                                                                                   >  2:11939/1‑100 (MQ=255)
            ttGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAAt                                                                                  <  2:1816298/100‑1 (MQ=255)
             tGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATg                                                                                 <  1:105161/100‑1 (MQ=255)
             tGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATg                                                                                 >  2:276578/1‑100 (MQ=255)
             tGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCCTCGAAAAACTCGGTATGGCCGGTAAAAGGAATg                                                                                 >  2:2296244/1‑100 (MQ=255)
              gCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGc                                                                                >  2:1564947/1‑100 (MQ=255)
               ccAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGcc                                                                               <  2:1994442/100‑1 (MQ=255)
                cAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAACACTCGGTATGGCCGGTAAAAGGAATGCCa                                                                              >  2:1450532/1‑100 (MQ=255)
                  gcgcCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGcc                                                                               <  2:2204709/97‑1 (MQ=255)
                   cgcCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGcg                                                                           <  1:346448/100‑1 (MQ=255)
                   cgcCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGcg                                                                           <  1:633609/100‑1 (MQ=255)
                     ccACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGcgtc                                                                         >  2:2030878/1‑100 (MQ=255)
                      cACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGcgtcg                                                                        >  2:1830338/1‑100 (MQ=255)
                        cgcgAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGtt                                                                      <  1:113725/100‑1 (MQ=255)
                            aaGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTtaata                                                                  <  1:409782/100‑1 (MQ=255)
                            aaGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTtaata                                                                  <  1:1246134/100‑1 (MQ=255)
                             aGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTtaataa                                                                 <  1:1257436/100‑1 (MQ=255)
                             aGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTtaataa                                                                 >  1:2273735/1‑100 (MQ=255)
                              gTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAAc                                                                >  1:972256/1‑100 (MQ=255)
                                tcttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGc                                                              >  1:1586287/1‑100 (MQ=255)
                                tcttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGc                                                              >  1:1951937/1‑100 (MQ=255)
                                 cttcGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGcc                                                             >  1:821693/1‑100 (MQ=255)
                                    cGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCttt                                                          >  1:1455906/1‑100 (MQ=255)
                                    cGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCttt                                                          <  2:1863861/100‑1 (MQ=255)
                                     ggTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa                                                         <  1:2423606/100‑1 (MQ=255)
                                     ggTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa                                                         <  1:2110735/100‑1 (MQ=255)
                                     ggTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTa                                                         >  1:1116285/1‑100 (MQ=255)
                                       tCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATg                                                       <  2:1830393/100‑1 (MQ=255)
                                          ccAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCAc                                                    <  1:508750/100‑1 (MQ=255)
                                           cAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCAcc                                                   <  2:228961/100‑1 (MQ=255)
                                             gCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGCATGCCAGCGTCGTTAATAACGCCTTTATGCACCgg                                                 >  1:8509/1‑100 (MQ=255)
                                             gCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCgg                                                 <  2:1943592/100‑1 (MQ=255)
                                             gCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCgg                                                 <  1:1694445/100‑1 (MQ=255)
                                                tcatcaCTACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGa                                                <  2:886162/98‑1 (MQ=255)
                                                tcatcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGAcc                                              <  1:1641887/100‑1 (MQ=255)
                                                tcatcaCCACCTTTTTCGCCTGCGAACGATCTTCGCAACACTCGGTATGGCCGGTCAAAGGCATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGAc                                               <  2:2430217/99‑1 (MQ=255)
                                                  atcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGAcccg                                            <  2:315796/100‑3 (MQ=255)
                                                  atcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtg                                            >  2:1130399/1‑100 (MQ=255)
                                                  atcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTCTATGCACCGGACCtg                                            >  1:1579987/1‑100 (MQ=255)
                                                  atcaCCACCTTTTACGCCTGCGACCGCTCTTCGAAAAACTCGGTATGGCCGGTAACAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGa                                                <  2:886163/96‑1 (MQ=255)
                                                   tcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgt                                           <  1:1658150/100‑1 (MQ=255)
                                                   tcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgt                                           <  2:1310272/100‑1 (MQ=255)
                                                   tcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgt                                           >  1:2041616/1‑100 (MQ=255)
                                                   tcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgt                                           <  1:1625789/100‑1 (MQ=255)
                                                   tcaCCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCtgt                                           >  1:1479822/1‑100 (MQ=255)
                                                    caccacCCTTTTCGCCTGCGAACGCCCTTCGAAACACTCGCTATGGCCGGTAAAAGCAATCCCAGCGCCGTTAATAACGCCTTTAGgcaccgcacc                                              >  2:1874221/1‑96 (MQ=255)
                                                     accaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATCGCCGGTAAAAGGAATGCCAGCGTCGTTACTAACGCCTTTATGCACCGGACCtgtg                                          >  2:1478881/1‑99 (MQ=255)
                                                      ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                        <  2:137490/100‑1 (MQ=255)
                                                      ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                        >  1:1894557/1‑100 (MQ=255)
                                                      ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                        <  2:2320589/100‑1 (MQ=255)
                                                      ccaccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGAt                                        <  1:325169/100‑1 (MQ=255)
                                                       caccTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATc                                       <  1:1717097/100‑1 (MQ=255)
                                                        accTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGACTGCCAGCGTCGTTAATAACGCCTTTATACACCGGACCTGTGATc                                       >  2:533831/1‑99 (MQ=255)
                                                          cTTTTTCGCCTGCGAACGCTCTTCGAAAGACTCGGTATGTCCGGGAAAAGGGATGCCAGCGTCGTTAATAGCGCCTTTATGCACCGGACCTGTGATCAg                                     <  2:31173/99‑1 (MQ=255)
                                                            ttttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAgcgc                                  <  1:1122482/100‑1 (MQ=255)
                                                            ttttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAgcg                                   <  1:413329/99‑1 (MQ=255)
                                                              ttCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCgg                                <  1:951559/100‑1 (MQ=255)
                                                                cGCCTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCa                              >  1:2327371/1‑100 (MQ=255)
                                                                  ccTGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCaaa                            <  1:1697559/100‑1 (MQ=255)
                                                                    tGCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAAtt                          >  1:2538999/1‑100 (MQ=255)
                                                                     gCGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTc                         <  2:135177/100‑1 (MQ=255)
                                                                      cGAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCg                        <  1:259808/100‑1 (MQ=255)
                                                                       gAACGCTCTTCGAAAAACTCGGTATGGCCTGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCTCCGGACCTGTGATCAGCGCGGCAAATTCg                        <  2:622702/99‑1 (MQ=255)
                                                                       gAACGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGc                       <  1:1407611/100‑1 (MQ=255)
                                                                         aCGCTCTTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCg                     <  1:461584/100‑1 (MQ=255)
                                                                             tctTCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCa                 <  2:363209/100‑1 (MQ=255)
                                                                               ttCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGa               <  2:2446569/100‑1 (MQ=255)
                                                                                tCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGAc              >  2:838013/1‑100 (MQ=255)
                                                                                tCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGAc              >  2:1094721/1‑100 (MQ=255)
                                                                                tCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGAc              >  1:2004333/1‑100 (MQ=255)
                                                                                tCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGAc              <  1:2340709/100‑1 (MQ=255)
                                                                                tCGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGAc              >  1:858967/1‑100 (MQ=255)
                                                                                 cGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACa             >  2:2203415/1‑100 (MQ=255)
                                                                                 cGAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACa             <  1:1419586/100‑1 (MQ=255)
                                                                                  gAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACaa            >  1:1760863/1‑100 (MQ=255)
                                                                                  gAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACaa            >  2:2317637/1‑100 (MQ=255)
                                                                                  gAAAAACTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACaa            >  2:469292/1‑100 (MQ=255)
                                                                                   aaaaaCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAAc           >  2:54525/1‑100 (MQ=255)
                                                                                     aaaCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCa         <  1:1920361/100‑1 (MQ=255)
                                                                                       aCTCGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATc       >  2:1045215/1‑100 (MQ=255)
                                                                                          cGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACGTGAGATCAGAGCGGCAAATTCGCCGTTCAGACAACCATCGCa    >  2:1798711/1‑100 (MQ=255)
                                                                                          cGGTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCa    <  1:1950498/100‑1 (MQ=255)
                                                                                           ggTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCAc   <  2:1293514/100‑1 (MQ=255)
                                                                                            gTATGGCCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACg  <  2:169146/100‑1 (MQ=255)
                                                                                                 |                                                                                              
GTAAATGCGTCGTTGCCAGCGCCACGCGAAGTTCTTCGGTCGCCAGCATCATCACCACCTTTTTCGCCTGCGAACGCTCTTCGAAAAACTCGGTATGACCGGTAAAAGGAATGCCAGCGTCGTTAATAACGCCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGCCGTTCAGACAACCATCGCACG  >  NC_000913/52915‑53106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑