BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	691,390	G→C	16.2%	A132G (GCC→GGC)	ybeX ←	putative ion transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	691,390	0	G	C	16.2%	277.8 / 46.3	105	A132G (GCC→GGC)	ybeX	putative ion transport
Reads supporting (aligned to +/- strand): ref base G (47/41); new base C (9/8); total (56/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.63e-01

Only 100 of 106 total aligned reads displayed.

TGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGG  >  NC_000913/691292‑691489

   ggTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTc                                                                                                 <  1:1045331/100‑1 (MQ=255)

   ggTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTc                                                                                                 >  2:1218433/1‑100 (MQ=255)

     tAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAg                                                                                               >  1:1530637/1‑100 (MQ=255)

      aaCGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGc                                                                                              >  1:1844216/1‑100 (MQ=255)

        cGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCAt                                                                                            >  1:2406335/1‑100 (MQ=255)

         gCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATc                                                                                           <  1:347770/100‑1 (MQ=255)

         gCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAtg                                                                                           <  2:801531/100‑2 (MQ=255)

         gCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAtg                                                                                           <  1:2460537/100‑2 (MQ=255)

         gCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAtg                                                                                           <  1:2535084/100‑2 (MQ=255)

         gCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAtg                                                                                           <  1:181511/100‑2 (MQ=255)

          cTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAtgg                                                                                          <  2:230414/100‑3 (MQ=255)

          cTGAGAGCGAAACTCTTTCAGCATCCGGTCGACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCg                                                                                          <  2:1296039/100‑1 (MQ=255)

          cTGAGAGCGAAACTCGTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTGACACTTTGTCCATGCTGAAGCCTTCAGCAtgg                                                                                          <  2:479499/100‑3 (MQ=255)

           tGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAtgga                                                                                         <  2:2367071/100‑4 (MQ=255)

            gagaGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGCCTTCAGCAtggac                                                                                        <  2:2204750/100‑5 (MQ=255)

                gCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTgcgc                                                                                    <  1:1316199/100‑1 (MQ=255)

                gCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTgcg                                                                                     <  2:2355397/99‑1 (MQ=255)

                 cGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCa                                                                                   <  1:2202023/100‑1 (MQ=255)

                 cGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCa                                                                                   <  2:2101614/100‑1 (MQ=255)

                 cGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCa                                                                                   >  2:1507738/1‑100 (MQ=255)

                 cGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCa                                                                                   >  1:1275146/1‑100 (MQ=255)

                       tctTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACg                                                                             <  1:746928/100‑1 (MQ=255)

                       tctTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACg                                                                             <  1:1872168/100‑1 (MQ=255)

                         tttCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgc                                                                           >  2:564054/1‑100 (MQ=255)

                         tttCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgc                                                                           >  1:1618911/1‑100 (MQ=255)

                          ttCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgca                                                                          >  1:2011502/1‑100 (MQ=255)

                          tacAGCATCCGGTATACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgca                                                                          >  1:1728049/3‑100 (MQ=255)

                           tCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgcag                                                                         >  2:1956569/1‑100 (MQ=255)

                             aGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgcagca                                                                       >  1:2182999/1‑100 (MQ=255)

                             aGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgcagca                                                                       >  2:1266538/1‑100 (MQ=255)

                             aGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGgcagca                                                                       <  2:135036/100‑1 (MQ=255)

                              gCATCCGGTCTACGCGCTTACTTTCAGGAACGAAAACCGCCTGTCGTAACACTTTGTCCATGCTGAAGGCTTCTGCATCGCTGCGCATAAACGGCAGCaa                                                                      <  1:553384/100‑1 (MQ=255)

                                 tCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTc                                                                   >  2:1166607/1‑100 (MQ=255)

                                  ccGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCt                                                                  >  2:1771166/1‑100 (MQ=255)

                                   cGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGGAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCtt                                                                 >  2:346532/1‑100 (MQ=255)

                                    ggTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCttt                                                                >  2:1822281/1‑100 (MQ=255)

                                     gTCTACGCGCTTACTTTCAGGAACGAAAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTc                                                               <  2:385927/100‑1 (MQ=255)

                                      tCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCg                                                              <  2:1806122/100‑1 (MQ=255)

                                      tCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCg                                                              <  2:1972704/100‑1 (MQ=255)

                                      tCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCg                                                              <  1:534652/100‑1 (MQ=255)

                                          cgcgCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCAt                                                          >  1:313541/1‑100 (MQ=255)

                                          cgcgCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCAt                                                          >  1:1516784/1‑100 (MQ=255)

                                            cgcTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCa                                                        <  2:1274089/100‑1 (MQ=255)

                                             gcTTACTTTCAGGATCGACAACCGCCTGACGTAACTCTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAg                                                       <  1:2497616/100‑1 (MQ=255)

                                                 aCTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTCCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATc                                                   <  1:1804457/100‑1 (MQ=255)

                                                  cTTTCCGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGAATTGCTGGGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATcc                                                  >  2:1640724/1‑100 (MQ=255)

                                                  cTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCTATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATc                                                   >  2:1578709/1‑99 (MQ=255)

                                                  cTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATcc                                                  >  2:737753/1‑100 (MQ=255)

                                                  cTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATcc                                                  >  1:140242/1‑100 (MQ=255)

                                                  cTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATcc                                                  >  1:87055/1‑100 (MQ=255)

                                                    ttCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCt                                                >  1:1598785/1‑100 (MQ=255)

                                                     tCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCtt                                               <  1:540364/100‑1 (MQ=255)

                                                      cAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCCTTCGCCATCAGAATCCCTTc                                              <  2:341752/100‑1 (MQ=255)

                                                        ggAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCaa                                            >  1:1927670/1‑100 (MQ=255)

                                                        ggAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCaa                                            >  1:1471047/1‑100 (MQ=255)

                                                         gAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAAt                                           <  1:2551776/100‑1 (MQ=255)

                                                          aaCGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAAGCCCTTCAAtg                                          <  2:61017/100‑1 (MQ=255)

                                                           aCGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAAtgt                                         <  2:2297219/100‑1 (MQ=255)

                                                           aCGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAAtgt                                         >  1:1442888/1‑100 (MQ=255)

                                                            cGACAACCGCCTGACGTAACACTTGGTCCATGCCGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAAtgtg                                        <  2:1248877/100‑1 (MQ=255)

                                                             gACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGa                                       >  1:1715928/1‑100 (MQ=255)

                                                             gACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGa                                       >  1:1529761/1‑100 (MQ=255)

                                                              aCAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGAt                                      <  1:670997/100‑1 (MQ=255)

                                                              aCAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGAAAGTCTTTCGCCATCAGAATCCCTTCAATGTGAt                                      <  2:2517908/100‑1 (MQ=255)

                                                               cAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATc                                     >  2:1424687/1‑100 (MQ=255)

                                                                aaCCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCt                                    >  1:2000315/1‑100 (MQ=255)

                                                                 aCCGCCTGACGTAACACTTTCTTCATGCTGAAGGCTTCCGCATCGCTGCGCATAAACGGCAGCAGGTCTTTCGCCATCAGAGTCCCTTCAATGTGATCtt                                   >  2:1009436/1‑100 (MQ=255)

                                                                   cGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAATCGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTg                                 <  2:196025/100‑1 (MQ=255)

                                                                   cGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTg                                 <  2:109806/100‑1 (MQ=255)

                                                                   cGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTg                                 >  1:1090838/1‑100 (MQ=255)

                                                                       tGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCtt                             <  1:2146046/100‑1 (MQ=255)

                                                                       tGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCtt                             <  1:330434/100‑1 (MQ=255)

                                                                          cGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGc                          <  2:392601/100‑1 (MQ=255)

                                                                          cGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTATTCGc                          >  1:1490934/1‑100 (MQ=255)

                                                                            tAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCt                         <  1:724930/99‑1 (MQ=255)

                                                                             aaCACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTaa                       <  2:1023963/100‑1 (MQ=255)

                                                                              acacTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAAt                      >  1:2470167/1‑100 (MQ=255)

                                                                                 cTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCAc                   >  1:1204754/1‑100 (MQ=255)

                                                                                 cTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCAc                   <  1:61724/100‑1 (MQ=255)

                                                                                 cTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCAc                   >  1:341866/1‑100 (MQ=255)

                                                                                  cgcagcgaTGCTGAAGCCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCAcc                  >  1:1830855/8‑100 (MQ=39)

                                                                                    tGTCCATGCTTAAGGCTTCAGCATCGCTGCTCATGAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTACAATGTTATCTTTGTCTTCGCTAATCACCg                 <  2:2131123/99‑1 (MQ=255)

                                                                                    cagcgaTGCTGAAGCCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCgg                >  1:1779947/6‑100 (MQ=255)

                                                                                     agcgaTGCTGAAGCCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCggg               >  2:1396676/5‑100 (MQ=255)

                                                                                     agcgaTGCTGAAGCCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCggg               >  1:1263812/5‑100 (MQ=255)

                                                                                      gcgaTGCTGAAGCCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGa              >  1:1484848/4‑100 (MQ=255)

                                                                                        gaTGCTGAAGCCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGaaa            >  2:1695473/2‑100 (MQ=255)

                                                                                        gaTGCTGAAGCCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGaaa            >  1:1063837/2‑100 (MQ=255)

                                                                                         aTGCTGAAGCCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAAc           >  2:1682393/1‑100 (MQ=255)

                                                                                          tGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACg          >  1:339051/1‑100 (MQ=255)

                                                                                          tGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACg          >  2:2522542/1‑100 (MQ=255)

                                                                                            cTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTg        <  2:1034083/100‑1 (MQ=255)

                                                                                             tGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGa       <  1:2031483/100‑1 (MQ=255)

                                                                                             tGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGa       >  1:1905129/1‑100 (MQ=255)

                                                                                              gAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGa       >  2:2211712/1‑99 (MQ=255)

                                                                                              gAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAg      >  2:366932/1‑100 (MQ=255)

                                                                                              gAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAg      <  1:887015/100‑1 (MQ=255)

                                                                                              gAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATACCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAg      >  2:198693/1‑100 (MQ=255)

                                                                                                aGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTg    <  1:2197412/100‑1 (MQ=255)

                                                                                                  gCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTggg  >  2:1941859/1‑100 (MQ=255)

TGTGGTAACGCTGAGAGCGAAACTCTTTCAGCATCCGGTCTACGCGCTTACTTTCAGGAACGACAACCGCCTGACGTAACACTTTGTCCATGCTGAAGGCTTCAGCATCGCTGCGCATAAACGGCAGCAAGTCTTTCGCCATCAGAATCCCTTCAATGTGATCTTTGTCTTCGCTAATCACCGGGAAACGTGAGTGGG  >  NC_000913/691292‑691489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`