Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 710,170 C→T 18.8% intergenic (+54/+30) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913710,1700CT18.8% 313.2 / 57.3 142intergenic (+54/+30)chiQ/furchitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (58/57);  new base T (12/15);  total (70/72)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 149 total aligned reads displayed.

TGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGA  >  NC_000913/710075‑710269
                                                                                               |                                                                                                   
tgtgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCa                                                                                                  <  1:2381005/99‑1 (MQ=255)
tgtgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAg                                                                                                 <  1:2031757/100‑1 (MQ=255)
tgtcATAAAGTCTAGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCa                                                                                                  <  2:2491277/99‑1 (MQ=255)
 gtgATAAAGTTTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAg                                                                                                 <  2:553326/99‑1 (MQ=255)
  tgATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGt                                                                                               >  1:1698646/1‑100 (MQ=255)
   gATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGtt                                                                                              >  1:355890/1‑100 (MQ=255)
      agagcctagCAGGAAATACCCACTCATAACCAATCACTGAGAGCTTTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAAccc                                                                                                     >  2:2164554/9‑90 (MQ=255)
      aaaGTCTGGCAGGAAATACGCAGTAATAACAAATAAGGGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGc                                                                                           <  2:2290119/100‑1 (MQ=255)
        aGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGTGGGTTGGCtt                                                                                         <  1:627927/100‑1 (MQ=255)
        aGTCTGGCAAGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCtt                                                                                         <  2:2322365/100‑1 (MQ=255)
           ntGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                      >  2:944354/2‑100 (MQ=255)
           cTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTGCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCtttgc                                                                                      >  1:1016359/1‑98 (MQ=255)
           cTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttt                                                                                      <  2:1823403/100‑2 (MQ=255)
           cTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                      <  1:1386918/100‑1 (MQ=255)
           cTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                      <  1:2284288/100‑1 (MQ=255)
           cTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtc                                                                                      <  2:732336/100‑1 (MQ=255)
            tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct                                                                                     >  1:146376/1‑100 (MQ=255)
            tGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTtct                                                                                     <  1:1446317/100‑1 (MQ=255)
             ggCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCtttttta                                                                                    <  2:355886/100‑4 (MQ=39)
             ggCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCtttttta                                                                                    <  2:1020453/100‑4 (MQ=39)
              gCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttttat                                                                                   <  2:2428809/100‑5 (MQ=38)
               cAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttttatg                                                                                  <  1:297154/100‑6 (MQ=38)
               cAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttttatg                                                                                  <  1:1862932/100‑6 (MQ=38)
               cAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttttatg                                                                                  <  1:1862959/100‑6 (MQ=38)
                aGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGtt                                                                                 <  1:1286403/100‑1 (MQ=255)
                 ggAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTc                                                                                >  2:2180264/1‑100 (MQ=255)
                 ggAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTc                                                                                >  2:1391841/1‑100 (MQ=255)
                   aaaTACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCTGCGGGTTGGCttttttatgcaag                                                                              <  1:617222/100‑10 (MQ=37)
                      tACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggct                                                                           >  1:1327662/1‑100 (MQ=255)
                       aCGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAgactg                                                                          >  2:1824335/1‑100 (MQ=255)
                        cGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctgg                                                                         <  2:2067158/100‑1 (MQ=255)
                        cGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggct                                                                           >  2:802912/1‑98 (MQ=255)
                         gCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctggc                                                                        <  1:691158/100‑1 (MQ=255)
                          cAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctggct                                                                       >  2:1484928/1‑100 (MQ=255)
                          cAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctggct                                                                       <  1:2022226/100‑1 (MQ=255)
                          cAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctggct                                                                       >  2:116936/1‑100 (MQ=255)
                          cAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAggctggct                                                                       >  1:2000422/1‑100 (MQ=255)
                            gTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTa                                                                     <  1:868298/100‑1 (MQ=255)
                              aataaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTAtt                                                                   <  1:2424256/100‑1 (MQ=255)
                                taaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTg                                                                 >  1:1611615/1‑100 (MQ=255)
                                taaCAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCATTGCATAAAAAAGCCAACCCGTAGGTTTGCTTTTCTTGTTAAGGCCGGCCTATTTg                                                                 >  2:1614789/1‑100 (MQ=255)
                                   cAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCt                                                              <  2:96598/100‑1 (MQ=255)
                                      aTAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCg                                                           <  2:1962877/100‑1 (MQ=255)
                                        aaGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTg                                                         >  1:105731/1‑100 (MQ=255)
                                          gTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgcg                                                       <  2:550212/100‑1 (MQ=255)
                                          gTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgcg                                                       <  2:413995/100‑1 (MQ=255)
                                           tGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTgcgc                                                      >  1:1833929/1‑100 (MQ=255)
                                                gCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGtt                                                 <  2:1540185/100‑1 (MQ=255)
                                                gCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGtt                                                 <  2:152213/100‑1 (MQ=255)
                                                 cTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTc                                                >  2:956826/1‑100 (MQ=255)
                                                  tgcaaCTCACTCTTTTCTTATTTCCCTGTCATATAAAATCCAATCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTc                                                <  1:1981079/96‑1 (MQ=255)
                                                  tGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTGGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTcn                                               <  2:1371102/100‑2 (MQ=255)
                                                  tGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCa                                               <  2:1730051/100‑1 (MQ=255)
                                                  tGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCa                                               <  2:1313301/100‑1 (MQ=255)
                                                   gTACCTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCAt                                              <  2:1444232/100‑1 (MQ=255)
                                                    tAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATc                                             <  1:1000486/100‑1 (MQ=255)
                                                       ctctCGCTTGTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCtt                                           <  2:203732/99‑1 (MQ=255)
                                                          tcGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTcgcg                                       <  2:76585/100‑1 (MQ=255)
                                                           cGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCCTGTTCATATTcgcg                                       >  1:2137305/1‑99 (MQ=255)
                                                           cGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCgg                                      >  1:959294/1‑100 (MQ=255)
                                                             cTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCa                                    >  1:2044696/1‑100 (MQ=255)
                                                               tttCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTGCGCGGCAAt                                  >  2:2510931/1‑100 (MQ=255)
                                                                 tCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGACTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCg                                >  2:442073/1‑100 (MQ=255)
                                                                  cTTATTTCCCTTGCATAAAAAAGCCAACCCGCCGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTTCGCATGTTCATCTTCGCGGCAATCGc                               >  2:337185/1‑100 (MQ=255)
                                                                  cTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGc                               >  2:30710/1‑100 (MQ=255)
                                                                      tttCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTc                           >  1:697760/1‑100 (MQ=255)
                                                                      tttCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTc                           <  2:2119085/100‑1 (MQ=255)
                                                                       ttCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCg                          >  1:1928735/1‑100 (MQ=255)
                                                                        tCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCgg                         <  2:10138/100‑1 (MQ=255)
                                                                         cccTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGc                        <  1:248763/100‑1 (MQ=255)
                                                                          ccTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGca                       <  2:32227/100‑1 (MQ=255)
                                                                           cTTGAATAAAAAATCCAACCCGCAGGTTGGCTTTGCTCGTTCAGGCTGGCTTATTTGCCGTCGGGCGCATGTTCATATTCGCGGCAATCGCCTTCGGc                        <  2:1589864/98‑1 (MQ=255)
                                                                            ttGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGcaca                     <  2:1482316/100‑1 (MQ=255)
                                                                             tGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAg                    <  2:105729/100‑1 (MQ=255)
                                                                             tGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAAGCGCCTTCGGCACAg                    <  2:1752883/100‑1 (MQ=255)
                                                                              gCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGt                   >  2:2346950/1‑100 (MQ=255)
                                                                               cATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAggg                  >  1:1320209/1‑98 (MQ=255)
                                                                               cATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTg                  >  1:666105/1‑100 (MQ=255)
                                                                               cATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTg                  >  1:1362451/1‑100 (MQ=255)
                                                                                cgagaaaaGCCAACCTGCGGGTTGGCTTTTTTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGa                 >  1:929322/5‑100 (MQ=38)
                                                                                aTAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGa                 >  1:1230240/1‑100 (MQ=255)
                                                                                 tAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAc                >  1:2170644/1‑100 (MQ=255)
                                                                                 gagaaaaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAc                >  1:1579183/4‑100 (MQ=255)
                                                                                  aaaaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAca               >  1:1750470/1‑99 (MQ=255)
                                                                                  aaaaaaGCCAACCCGCAGGTTGGCTTTTCTCGTACAGGCTGGCTTATTTGCCTTCGTGCTCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGAc                >  2:1020020/1‑99 (MQ=255)
                                                                                   aaaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCg              >  1:847685/1‑100 (MQ=255)
                                                                                   aaaaaGCCAACCCGCAGGTTGGCTTTTCGCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCg              >  2:874964/1‑100 (MQ=255)
                                                                                    aaaaGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGt             >  1:166281/1‑100 (MQ=255)
                                                                                     aaaGCCCACCTGCGGGTTGGCTTTTCTCGTTCAGGCTCGCTTATTTGCCTTCGTGCGCACGCTCCTCTTCGCGGCAATCGCCTTCGGCACAGTGAcc               >  2:720363/1‑97 (MQ=255)
                                                                                     aaaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa            >  2:1137434/1‑100 (MQ=255)
                                                                                     aaaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTa            >  1:2024131/1‑100 (MQ=255)
                                                                                      aaGCCAACCTGCGGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaa           >  1:64482/1‑100 (MQ=255)
                                                                                       aGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTaaa          <  1:2185412/100‑1 (MQ=255)
                                                                                        gCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAg         <  1:1781262/100‑1 (MQ=255)
                                                                                        gCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAg         >  1:1237216/1‑100 (MQ=255)
                                                                                         ccAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGa        >  1:446849/1‑100 (MQ=255)
                                                                                             cccGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATaga    <  1:808601/100‑1 (MQ=255)
                                                                                              ccGCAGGTTGGATTTTCTCGTTCAGGCTGGATTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATagag   <  1:116938/100‑1 (MQ=255)
                                                                                               cGCAGGTTGGTTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAgaga  >  1:1855121/1‑100 (MQ=255)
                                                                                               cGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAgaga  >  1:1941431/1‑100 (MQ=255)
                                                                                               |                                                                                                   
TGTGATAAAGTCTGGCAGGAAATACGCAGTAATAACAAATAAGTGAGAGCTGTAACTCTCGCTTTTCTTATTTCCCTTGCATAAAAAAGCCAACCCGCAGGTTGGCTTTTCTCGTTCAGGCTGGCTTATTTGCCTTCGTGCGCATGTTCATCTTCGCGGCAATCGCCTTCGGCACAGTGACCGTAAAGATAGAGA  >  NC_000913/710075‑710269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: